Mutagenetix > Incidental Mutation

Incidental Mutation 'R5351:Vmn2r24'

423798

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r24

Ensembl Gene

ENSMUSG00000072780

Gene Name

vomeronasal 2, receptor 24

Synonyms

EG243628

MMRRC Submission

042930-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R5351 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123755930-123793239 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 123793223 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Methionine at position 850 (K850M)

Ref Sequence

ENSEMBL: ENSMUSP00000074602 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075095]

AlphaFold

D3YUI0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000075095
AA Change: K850M

PolyPhen 2 Score 0.795 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000074602
Gene: ENSMUSG00000072780
AA Change: K850M

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	82	469	1.6e-32	PFAM
Pfam:NCD3G	518	571	1.1e-22	PFAM
Pfam:7tm_3	602	839	1.1e-54	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.7%
20x: 96.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alpk1	T	A	3: 127,522,941 (GRCm39)	S34C	probably damaging	Het
Apol7e	T	G	15: 77,602,511 (GRCm39)	*370G	probably null	Het
Cacna2d4	T	C	6: 119,245,162 (GRCm39)	I290T	probably damaging	Het
Ceacam12	T	A	7: 17,801,159 (GRCm39)	V46D	probably damaging	Het
Ces1d	A	G	8: 93,904,706 (GRCm39)	Y345H	probably damaging	Het
Cirbp	T	C	10: 80,006,136 (GRCm39)		probably benign	Het
Cnot8	T	A	11: 58,006,147 (GRCm39)	H225Q	probably damaging	Het
Fstl1	T	C	16: 37,649,542 (GRCm39)	V252A	probably damaging	Het
H2-T3	T	C	17: 36,500,965 (GRCm39)	Q17R	probably benign	Het
Htt	A	G	5: 34,961,177 (GRCm39)	Y268C	probably damaging	Het
Ildr2	T	C	1: 166,136,478 (GRCm39)	V439A	possibly damaging	Het
Lig1	T	C	7: 13,034,875 (GRCm39)	M557T	probably damaging	Het
Ltbp2	T	C	12: 84,837,132 (GRCm39)	E1229G	possibly damaging	Het
Mynn	C	T	3: 30,661,691 (GRCm39)	R258W	probably benign	Het
Nbas	T	A	12: 13,610,850 (GRCm39)	N2180K	probably damaging	Het
Or8j3c	A	G	2: 86,253,610 (GRCm39)	S137P	probably damaging	Het
Pcdh1	C	T	18: 38,330,819 (GRCm39)	G728D	probably damaging	Het
Pfas	T	C	11: 68,882,217 (GRCm39)	D882G	probably damaging	Het
Prkar2b	C	T	12: 32,022,126 (GRCm39)	G60R	probably damaging	Het
Prkdc	G	C	16: 15,649,176 (GRCm39)	V3717L	probably benign	Het
Prom1	A	T	5: 44,201,697 (GRCm39)	V250E	probably damaging	Het
Slc35d1	A	G	4: 103,047,036 (GRCm39)	L254P	probably damaging	Het
Srrt	T	C	5: 137,296,546 (GRCm39)	*239W	probably null	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Ttn	T	A	2: 76,585,168 (GRCm39)	I22042F	probably damaging	Het
Ttn	A	G	2: 76,773,385 (GRCm39)	V2339A	probably damaging	Het
Unc80	T	A	1: 66,645,672 (GRCm39)	S1449R	possibly damaging	Het
Zfp516	A	G	18: 82,974,876 (GRCm39)	E358G	probably benign	Het

Other mutations in Vmn2r24

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r24	APN	6	123,792,596 (GRCm39)	missense	probably damaging	1.00
IGL01382:Vmn2r24	APN	6	123,763,938 (GRCm39)	missense	possibly damaging	0.62
IGL01592:Vmn2r24	APN	6	123,764,445 (GRCm39)	missense	probably benign	0.30
IGL01754:Vmn2r24	APN	6	123,781,120 (GRCm39)	missense	probably damaging	1.00
IGL01939:Vmn2r24	APN	6	123,764,404 (GRCm39)	missense	probably benign
IGL02140:Vmn2r24	APN	6	123,757,631 (GRCm39)	missense	probably damaging	0.98
IGL02272:Vmn2r24	APN	6	123,763,843 (GRCm39)	missense	possibly damaging	0.94
IGL02568:Vmn2r24	APN	6	123,792,812 (GRCm39)	missense	probably benign	0.36
IGL02748:Vmn2r24	APN	6	123,793,057 (GRCm39)	missense	possibly damaging	0.90
IGL03022:Vmn2r24	APN	6	123,755,967 (GRCm39)	missense	probably damaging	0.99
IGL03343:Vmn2r24	APN	6	123,793,070 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r24	UTSW	6	123,792,369 (GRCm39)	frame shift	probably null
R0453:Vmn2r24	UTSW	6	123,757,350 (GRCm39)	critical splice acceptor site	probably null
R0538:Vmn2r24	UTSW	6	123,793,012 (GRCm39)	missense	probably benign	0.32
R0607:Vmn2r24	UTSW	6	123,763,893 (GRCm39)	missense	probably benign
R1381:Vmn2r24	UTSW	6	123,763,692 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn2r24	UTSW	6	123,783,479 (GRCm39)	splice site	probably benign
R1848:Vmn2r24	UTSW	6	123,793,183 (GRCm39)	missense	probably damaging	1.00
R2035:Vmn2r24	UTSW	6	123,793,019 (GRCm39)	missense	probably damaging	1.00
R2077:Vmn2r24	UTSW	6	123,792,358 (GRCm39)	missense	probably damaging	1.00
R2122:Vmn2r24	UTSW	6	123,792,353 (GRCm39)	missense	possibly damaging	0.81
R2145:Vmn2r24	UTSW	6	123,755,972 (GRCm39)	missense	probably benign
R2483:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R2512:Vmn2r24	UTSW	6	123,763,985 (GRCm39)	missense	probably benign	0.01
R3001:Vmn2r24	UTSW	6	123,781,231 (GRCm39)	missense	probably benign	0.00
R3002:Vmn2r24	UTSW	6	123,781,231 (GRCm39)	missense	probably benign	0.00
R3236:Vmn2r24	UTSW	6	123,755,984 (GRCm39)	nonsense	probably null
R3623:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R3624:Vmn2r24	UTSW	6	123,792,997 (GRCm39)	missense	probably damaging	1.00
R3835:Vmn2r24	UTSW	6	123,764,412 (GRCm39)	missense	probably benign	0.33
R4074:Vmn2r24	UTSW	6	123,764,374 (GRCm39)	missense	possibly damaging	0.92
R4075:Vmn2r24	UTSW	6	123,764,374 (GRCm39)	missense	possibly damaging	0.92
R4812:Vmn2r24	UTSW	6	123,756,144 (GRCm39)	missense	probably benign	0.00
R4825:Vmn2r24	UTSW	6	123,792,739 (GRCm39)	missense	probably benign	0.02
R5665:Vmn2r24	UTSW	6	123,763,938 (GRCm39)	missense	possibly damaging	0.62
R5790:Vmn2r24	UTSW	6	123,792,499 (GRCm39)	missense	probably benign
R5808:Vmn2r24	UTSW	6	123,792,597 (GRCm39)	nonsense	probably null
R5879:Vmn2r24	UTSW	6	123,764,226 (GRCm39)	missense	possibly damaging	0.89
R5923:Vmn2r24	UTSW	6	123,792,751 (GRCm39)	missense	probably damaging	0.96
R5969:Vmn2r24	UTSW	6	123,755,981 (GRCm39)	missense	probably benign	0.00
R6050:Vmn2r24	UTSW	6	123,792,691 (GRCm39)	missense	probably damaging	1.00
R6171:Vmn2r24	UTSW	6	123,764,205 (GRCm39)	missense	probably damaging	0.98
R6174:Vmn2r24	UTSW	6	123,793,236 (GRCm39)	missense	probably benign	0.00
R6356:Vmn2r24	UTSW	6	123,783,368 (GRCm39)	missense	possibly damaging	0.93
R6562:Vmn2r24	UTSW	6	123,757,386 (GRCm39)	missense	probably benign	0.01
R6563:Vmn2r24	UTSW	6	123,781,137 (GRCm39)	missense	possibly damaging	0.86
R6584:Vmn2r24	UTSW	6	123,792,764 (GRCm39)	missense	possibly damaging	0.53
R6630:Vmn2r24	UTSW	6	123,763,981 (GRCm39)	missense	probably benign	0.00
R6803:Vmn2r24	UTSW	6	123,755,960 (GRCm39)	missense	possibly damaging	0.64
R6864:Vmn2r24	UTSW	6	123,756,117 (GRCm39)	missense	possibly damaging	0.89
R7252:Vmn2r24	UTSW	6	123,764,191 (GRCm39)	missense	possibly damaging	0.90
R7369:Vmn2r24	UTSW	6	123,792,638 (GRCm39)	missense	probably damaging	0.99
R7646:Vmn2r24	UTSW	6	123,793,169 (GRCm39)	missense	probably benign	0.20
R7799:Vmn2r24	UTSW	6	123,757,422 (GRCm39)	missense	probably benign	0.00
R7803:Vmn2r24	UTSW	6	123,757,438 (GRCm39)	missense	probably benign	0.00
R7959:Vmn2r24	UTSW	6	123,755,949 (GRCm39)	missense	possibly damaging	0.86
R8215:Vmn2r24	UTSW	6	123,756,077 (GRCm39)	missense	probably benign	0.10
R8796:Vmn2r24	UTSW	6	123,757,500 (GRCm39)	missense	probably benign
R9172:Vmn2r24	UTSW	6	123,783,432 (GRCm39)	missense	probably damaging	1.00
R9300:Vmn2r24	UTSW	6	123,793,030 (GRCm39)	missense	possibly damaging	0.46
R9369:Vmn2r24	UTSW	6	123,792,357 (GRCm39)	missense	probably damaging	1.00
R9375:Vmn2r24	UTSW	6	123,792,542 (GRCm39)	missense	probably damaging	1.00
R9523:Vmn2r24	UTSW	6	123,763,950 (GRCm39)	missense	possibly damaging	0.89
R9546:Vmn2r24	UTSW	6	123,764,266 (GRCm39)	missense	probably damaging	0.98
RF006:Vmn2r24	UTSW	6	123,783,378 (GRCm39)	missense	probably damaging	1.00
RF016:Vmn2r24	UTSW	6	123,781,174 (GRCm39)	missense	probably benign	0.04
X0023:Vmn2r24	UTSW	6	123,764,359 (GRCm39)	missense	probably damaging	0.99
Z1088:Vmn2r24	UTSW	6	123,781,155 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn2r24	UTSW	6	123,763,719 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TCAGCATGCTGGTTTTCTGC -3'
(R):5'- AAGTCACTTCTAGTTGGCAATGATC -3'

Sequencing Primer
(F):5'- GCAGTGTGTGGATTTCTTTCATACCC -3'
(R):5'- GGCAATGATCTAAACTTTGATGGC -3'

Posted On

2016-08-04