Mutagenetix > Incidental Mutation

Incidental Mutation 'R5353:Tas2r109'

423899

Institutional Source

Beutler Lab

Gene Symbol

Tas2r109

Ensembl Gene

ENSMUSG00000062528

Gene Name

taste receptor, type 2, member 109

Synonyms

mt2r62, T2R09, mGR09, Tas2r9

MMRRC Submission

042932-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.053) question?

Stock #

R5353 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

132956978-132957928 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132957594 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 112 (V112A)

Ref Sequence

ENSEMBL: ENSMUSP00000069300 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067539]

AlphaFold

Q7M707

Predicted Effect

possibly damaging
Transcript: ENSMUST00000067539
AA Change: V112A

PolyPhen 2 Score 0.606 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000069300
Gene: ENSMUSG00000062528
AA Change: V112A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	9	309	4.3e-81	PFAM

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.6%
20x: 96.2%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ablim3	A	G	18: 61,934,470 (GRCm39)	S649P	probably damaging	Het
Adam22	C	A	5: 8,140,182 (GRCm39)	G202W	probably damaging	Het
Adamts1	T	A	16: 85,599,496 (GRCm39)	M35L	probably benign	Het
Adgrg3	A	T	8: 95,762,556 (GRCm39)	H202L	probably damaging	Het
Anln	G	T	9: 22,271,813 (GRCm39)	R681S	probably damaging	Het
Aprt	A	T	8: 123,302,147 (GRCm39)	M1K	probably null	Het
Arid3b	G	T	9: 57,702,320 (GRCm39)		probably null	Het
Cbl	A	G	9: 44,084,620 (GRCm39)	F172L	probably damaging	Het
Cd109	G	T	9: 78,617,521 (GRCm39)	V1340L	probably damaging	Het
Cdc45	C	T	16: 18,614,647 (GRCm39)	R205H	probably damaging	Het
Chrm3	A	G	13: 9,928,593 (GRCm39)	Y148H	probably damaging	Het
Cog7	A	C	7: 121,540,470 (GRCm39)		probably null	Het
Cpsf1	A	T	15: 76,486,771 (GRCm39)	I255N	probably damaging	Het
Crebzf	G	A	7: 90,092,622 (GRCm39)	G134R	probably damaging	Het
Crybg1	T	C	10: 43,849,661 (GRCm39)	S1705G	probably damaging	Het
Dock6	A	T	9: 21,726,082 (GRCm39)	H1409Q	probably benign	Het
Fank1	A	G	7: 133,478,632 (GRCm39)	D232G	probably damaging	Het
Fat1	G	T	8: 45,489,168 (GRCm39)	V3480L	probably benign	Het
Fchsd1	C	T	18: 38,092,926 (GRCm39)		probably benign	Het
Fmn2	G	T	1: 174,330,572 (GRCm39)	G321W	unknown	Het
Greb1	A	T	12: 16,738,567 (GRCm39)	Y1465*	probably null	Het
Hdac9	G	A	12: 34,443,392 (GRCm39)	Q330*	probably null	Het
Kcnt2	C	T	1: 140,354,639 (GRCm39)	T298I	probably damaging	Het
Knl1	A	G	2: 118,901,464 (GRCm39)	D1055G	probably benign	Het
Mroh2b	T	C	15: 4,946,660 (GRCm39)	S487P	probably damaging	Het
Naa38	G	A	11: 69,287,408 (GRCm39)	V110I	probably benign	Het
Nkd2	T	C	13: 73,969,557 (GRCm39)	H303R	probably damaging	Het
Nr1d2	A	G	14: 18,222,125 (GRCm38)	C49R	probably benign	Het
Or4c111	A	G	2: 88,844,099 (GRCm39)	V103A	probably benign	Het
Or52b2	A	G	7: 104,986,324 (GRCm39)	Y200H	probably damaging	Het
Ovch2	T	A	7: 107,393,631 (GRCm39)	E165V	probably damaging	Het
Phyh	A	T	2: 4,947,012 (GRCm39)		probably benign	Het
Pik3r4	G	A	9: 105,545,137 (GRCm39)		probably null	Het
Ppip5k1	A	C	2: 121,142,201 (GRCm39)	V1416G	probably benign	Het
Ppm1b	T	C	17: 85,301,537 (GRCm39)	V139A	probably benign	Het
Ppp1r12a	T	G	10: 108,097,077 (GRCm39)		probably null	Het
Pramel20	A	G	4: 143,297,807 (GRCm39)	T76A	probably benign	Het
Psmc5	G	T	11: 106,152,327 (GRCm39)	A115S	probably damaging	Het
Ptpn5	T	A	7: 46,731,642 (GRCm39)	E409V	probably benign	Het
Ptprg	T	A	14: 11,554,235 (GRCm38)		probably benign	Het
Qrich1	G	T	9: 108,422,164 (GRCm39)	V593F	probably damaging	Het
Rbbp9	T	C	2: 144,385,741 (GRCm39)	I175V	probably benign	Het
Selenot	T	C	3: 58,493,387 (GRCm39)	F88S	possibly damaging	Het
Sp110	C	T	1: 85,516,841 (GRCm39)	E219K	possibly damaging	Het
Spred2	A	G	11: 19,968,155 (GRCm39)	D208G	possibly damaging	Het
Surf1	T	C	2: 26,804,204 (GRCm39)	T197A	probably benign	Het
Taco1	G	A	11: 105,963,539 (GRCm39)		probably null	Het
Tpr	C	T	1: 150,321,675 (GRCm39)	R3C	probably damaging	Het
Uggt2	A	G	14: 119,319,182 (GRCm39)	I280T	probably benign	Het
Yipf2	A	G	9: 21,503,228 (GRCm39)	Y80H	possibly damaging	Het
Zscan12	T	C	13: 21,548,178 (GRCm39)	V120A	possibly damaging	Het

Other mutations in Tas2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00467:Tas2r109	APN	6	132,956,986 (GRCm39)	missense	probably benign	0.04
IGL01837:Tas2r109	APN	6	132,957,477 (GRCm39)	missense	probably benign	0.27
IGL02094:Tas2r109	APN	6	132,957,202 (GRCm39)	missense	possibly damaging	0.78
R0788:Tas2r109	UTSW	6	132,957,264 (GRCm39)	missense	probably benign	0.01
R0849:Tas2r109	UTSW	6	132,957,856 (GRCm39)	missense	probably benign	0.00
R1542:Tas2r109	UTSW	6	132,957,873 (GRCm39)	missense	possibly damaging	0.93
R1583:Tas2r109	UTSW	6	132,957,389 (GRCm39)	missense	probably benign	0.01
R2035:Tas2r109	UTSW	6	132,957,423 (GRCm39)	missense	probably benign
R3845:Tas2r109	UTSW	6	132,957,766 (GRCm39)	missense	probably damaging	0.99
R4060:Tas2r109	UTSW	6	132,957,148 (GRCm39)	missense	probably damaging	1.00
R4355:Tas2r109	UTSW	6	132,957,144 (GRCm39)	missense	probably benign
R5860:Tas2r109	UTSW	6	132,957,664 (GRCm39)	missense	probably benign	0.06
R6211:Tas2r109	UTSW	6	132,957,587 (GRCm39)	nonsense	probably null
R6378:Tas2r109	UTSW	6	132,957,844 (GRCm39)	missense	probably benign	0.00
R6861:Tas2r109	UTSW	6	132,957,048 (GRCm39)	missense	probably benign	0.43
R7319:Tas2r109	UTSW	6	132,957,663 (GRCm39)	missense	probably benign	0.33
R8553:Tas2r109	UTSW	6	132,957,171 (GRCm39)	missense	probably benign	0.33
R9447:Tas2r109	UTSW	6	132,957,270 (GRCm39)	missense	probably damaging	1.00
Z1088:Tas2r109	UTSW	6	132,957,264 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- CTTCCTAACACCTGAATTTGACAAC -3'
(R):5'- CACCGCTTTGGCAACTTCTAG -3'

Sequencing Primer
(F):5'- CACCTGAATTTGACAACTTAAATGG -3'
(R):5'- GGCAACTTCTAGAATTTTCCTGC -3'

Posted On

2016-08-04