Mutagenetix > Incidental Mutations

Incidental Mutation 'R5354:Wnk1'

423969

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

6430573H23Rik, Hsn2, EG406236, Prkwnk1

MMRRC Submission

042933-MU

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R5354 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

119923969-120038672 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 119968523 bp

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 699 (I699V)

Ref Sequence

ENSEMBL: ENSMUSP00000145304 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000161512] [ENSMUST00000177761] [ENSMUST00000203030]

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000035513

Predicted Effect

probably benign
Transcript: ENSMUST00000060043
AA Change: I699V

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: I699V

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088644
AA Change: I699V

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: I699V

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000088646
AA Change: I699V

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: I699V

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159151

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000159397

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000160769

Predicted Effect

unknown
Transcript: ENSMUST00000161048
AA Change: I287V

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962
AA Change: I287V

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000161392

Predicted Effect

probably benign
Transcript: ENSMUST00000161512

SMART Domains

Protein: ENSMUSP00000136086
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	40	51	N/A	INTRINSIC
low complexity region	217	233	N/A	INTRINSIC
low complexity region	348	361	N/A	INTRINSIC
low complexity region	365	374	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000162368
AA Change: I47V

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962
AA Change: I47V

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000177761
AA Change: I699V

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: I699V

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203030
AA Change: I699V

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: I699V

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

100% (80/80)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630010A05Rik	A	G	16: 14,618,671	K196R	probably benign	Het
Ablim1	T	C	19: 57,130,923	E243G	probably benign	Het
Acat1	T	A	9: 53,589,183	E271V	possibly damaging	Het
Aco1	T	C	4: 40,180,290		probably null	Het
Adam22	C	A	5: 8,090,182	G202W	probably damaging	Het
Agbl3	T	A	6: 34,814,752	H596Q	probably benign	Het
Anxa7	A	G	14: 20,464,909	L177P	possibly damaging	Het
Atp11a	A	T	8: 12,806,753	N48I	probably damaging	Het
Bcas1	T	A	2: 170,349,396	N492I	possibly damaging	Het
Bclaf1	A	G	10: 20,333,532	Y498C	probably damaging	Het
Bod1l	A	C	5: 41,831,537	V409G	probably damaging	Het
Ccdc170	G	A	10: 4,534,188	C338Y	probably benign	Het
Cdc45	C	T	16: 18,795,897	R205H	probably damaging	Het
Ckap2	A	C	8: 22,177,565	N93K	probably damaging	Het
Clca3a1	A	T	3: 144,737,005	S759R	possibly damaging	Het
Coro1c	A	T	5: 113,846,165	I347N	possibly damaging	Het
Cpox	A	G	16: 58,670,842	T139A	probably damaging	Het
Cyp4a12b	C	A	4: 115,433,464		probably null	Het
Dctn1	T	G	6: 83,183,126	V116G	possibly damaging	Het
Dhx38	A	G	8: 109,555,746	V683A	probably damaging	Het
Dnah12	G	A	14: 26,774,342		probably null	Het
Dnajb7	T	C	15: 81,408,007	E43G	probably damaging	Het
Dsc1	A	T	18: 20,087,575	V714E	probably damaging	Het
Dupd1	G	A	14: 21,677,023	R186W	probably benign	Het
Egfem1	T	C	3: 29,082,212		probably null	Het
Fchsd1	C	T	18: 37,959,873		probably benign	Het
Fmo1	T	C	1: 162,830,145	T476A	probably benign	Het
Gm10237	T	C	16: 35,920,729		noncoding transcript	Het
Gm14496	A	G	2: 182,000,809	S758G	probably damaging	Het
Gpat2	T	A	2: 127,428,723	L97Q	probably damaging	Het
Gpr162	T	C	6: 124,859,637	D357G	probably benign	Het
Hax1	A	T	3: 89,997,955	D34E	probably damaging	Het
Hmgcr	C	T	13: 96,654,896	V97M	probably benign	Het
Hsd3b2	T	C	3: 98,712,315	T105A	probably benign	Het
Ints1	A	G	5: 139,766,428		probably null	Het
Islr	T	C	9: 58,157,612	E204G	probably damaging	Het
Lrrc36	A	G	8: 105,425,364	N60D	probably damaging	Het
Maf1	T	C	15: 76,353,130		probably benign	Het
Mrgprb4	T	A	7: 48,198,329	R284W	probably benign	Het
Myh4	A	T	11: 67,255,725	N1508I	possibly damaging	Het
Nufip2	A	G	11: 77,686,277	H17R	unknown	Het
Oasl1	A	T	5: 114,936,996	I372L	probably damaging	Het
Olfr290	T	A	7: 84,916,149	Y123*	probably null	Het
Olfr414	T	C	1: 174,430,686	L86P	probably damaging	Het
Pald1	A	T	10: 61,348,661	Y226N	probably damaging	Het
Pcdhb13	G	T	18: 37,444,791	G741C	probably damaging	Het
Pcdhga10	A	G	18: 37,748,206	D340G	probably damaging	Het
Pclo	C	A	5: 14,678,808		probably benign	Het
Pcsk4	G	T	10: 80,323,689	N416K	probably damaging	Het
Pde10a	C	A	17: 8,961,980	R398S	probably damaging	Het
Plin1	T	A	7: 79,725,721	T227S	possibly damaging	Het
Pnpt1	A	G	11: 29,154,166	D542G	probably damaging	Het
Ppp4r3b	T	A	11: 29,211,646	D673E	probably benign	Het
Prr18	C	A	17: 8,341,060	P16Q	probably damaging	Het
Psmc3	T	A	2: 91,059,353	Y440N	probably damaging	Het
Rassf5	T	C	1: 131,180,648	I232V	probably benign	Het
Rims1	T	A	1: 22,538,511	D218V	probably damaging	Het
Skint10	T	C	4: 112,711,593	N309S	possibly damaging	Het
Slc6a1	T	C	6: 114,302,623	M121T	possibly damaging	Het
Slit3	A	G	11: 35,675,913	D1004G	probably damaging	Het
Snap91	C	T	9: 86,835,124	V215I	possibly damaging	Het
Son	T	C	16: 91,655,739	L458S	probably damaging	Het
St18	C	T	1: 6,844,171	A782V	probably damaging	Het
Synpo	A	G	18: 60,602,231		probably null	Het
Thbs3	A	G	3: 89,221,377	D458G	probably damaging	Het
Tpr	C	T	1: 150,445,924	R3C	probably damaging	Het
Trp63	A	G	16: 25,684,355		probably null	Het
Uhrf1bp1	G	A	17: 27,887,515	S1005N	probably benign	Het
Vmn1r27	C	A	6: 58,215,596	R141L	probably benign	Het
Xkr6	A	G	14: 63,818,904	D88G	possibly damaging	Het
Zan	A	G	5: 137,380,788		probably benign	Het
Zbtb7b	T	C	3: 89,379,606		probably benign	Het
Zfp618	A	T	4: 63,080,028	D33V	probably damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119960708	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119937411	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119948397	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119963485	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119949078	unclassified	probably benign
IGL02268:Wnk1	APN	6	119937373	nonsense	probably null
IGL02348:Wnk1	APN	6	119963328	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119963454	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119937862	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119926633	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119954187	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119953895	missense	probably benign	0.41
Actor	UTSW	6	119970978	missense	probably damaging	1.00
Bad	UTSW	6	119965678	intron	probably benign
Blink	UTSW	6	120002273	nonsense	probably null
Knock	UTSW	6	119943878	splice site	probably null
Narrow	UTSW	6	119992408	missense	probably damaging	1.00
nictitate	UTSW	6	120002280	missense	possibly damaging	0.88
opportunity	UTSW	6	119951068	missense	probably damaging	1.00
path	UTSW	6	120037149	missense	probably damaging	0.99
tear	UTSW	6	119963470	missense	probably damaging	0.98
Unforgiving	UTSW	6	119948282	missense	probably damaging	1.00
Window	UTSW	6	119992453	missense	probably damaging	1.00
woke	UTSW	6	119962955	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119944799	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119963367	nonsense	probably null
R0044:Wnk1	UTSW	6	120037149	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119952733	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119928163	intron	probably benign
R0453:Wnk1	UTSW	6	119963151	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119969332	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119962803	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119926564	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119951992	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119926683	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119948505	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119952006	splice site	probably benign
R1188:Wnk1	UTSW	6	119948709	nonsense	probably null
R1245:Wnk1	UTSW	6	119948457	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119950684	splice site	probably benign
R1869:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119951089	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119952923	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119969247	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119934382	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119937578	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119928157	splice site	probably null
R2144:Wnk1	UTSW	6	119948988	unclassified	probably benign
R2186:Wnk1	UTSW	6	119948567	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119963640	splice site	probably null
R2338:Wnk1	UTSW	6	119969534	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119936367	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119992453	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119950043	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119969354	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119949051	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119951068	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119948126	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119949261	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119953895	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119926426	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119951911	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119965744	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119963470	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119952771	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119962438	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119949377	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119965735	intron	probably benign
R5002:Wnk1	UTSW	6	119937963	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119965735	intron	probably benign
R5152:Wnk1	UTSW	6	120002280	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120037188	missense	probably benign	0.00
R5421:Wnk1	UTSW	6	119952818	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119948891	unclassified	probably benign
R5600:Wnk1	UTSW	6	119949358	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119992408	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120037601	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119972997	intron	probably benign
R6128:Wnk1	UTSW	6	119963786	unclassified	probably null
R6237:Wnk1	UTSW	6	119952767	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119948585	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119962955	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119948282	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119948781	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119965678	intron	probably benign
R7024:Wnk1	UTSW	6	119965726	intron	probably benign
R7072:Wnk1	UTSW	6	119937861	missense	unknown
R7087:Wnk1	UTSW	6	120037530	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119948307	missense	unknown
R7134:Wnk1	UTSW	6	119926428	missense	unknown
R7137:Wnk1	UTSW	6	120038212	unclassified	probably benign
R7142:Wnk1	UTSW	6	119949279	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119970978	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119943878	splice site	probably null
R7218:Wnk1	UTSW	6	120002273	nonsense	probably null
R7498:Wnk1	UTSW	6	119927196	missense	unknown
R7599:Wnk1	UTSW	6	119929828	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119932738	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119949176	missense	probably benign	0.04
X0064:Wnk1	UTSW	6	120037032	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119948205	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AGCGTTTCACTATTTAAACACCCC -3'
(R):5'- GCTCTGCAGATGATAACATTCAGTG -3'

Sequencing Primer
(F):5'- ACAATAGATATCGAGACTGACTAGAC -3'
(R):5'- AAATGTGAACAATTTCTTCCTCTTCC -3'

Posted On

2016-08-04