Mutagenetix > Incidental Mutation

Incidental Mutation 'R5290:Spen'

424644

Institutional Source

Beutler Lab

Gene Symbol

Spen

Ensembl Gene

ENSMUSG00000040761

Gene Name

spen family transcription repressor

Synonyms

Mint

MMRRC Submission

042873-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5290 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

141467890-141538597 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 141473816 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 2500 (T2500I)

Ref Sequence

ENSEMBL: ENSMUSP00000101412 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078886] [ENSMUST00000105786]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000078886
AA Change: T2477I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000077925
Gene: ENSMUSG00000040761
AA Change: T2477I

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	617	632	N/A	INTRINSIC
low complexity region	669	691	N/A	INTRINSIC
low complexity region	695	720	N/A	INTRINSIC
low complexity region	749	773	N/A	INTRINSIC
coiled coil region	800	825	N/A	INTRINSIC
low complexity region	830	841	N/A	INTRINSIC
internal_repeat_2	844	954	6.27e-5	PROSPERO
coiled coil region	1494	1522	N/A	INTRINSIC
low complexity region	1587	1627	N/A	INTRINSIC
low complexity region	1635	1641	N/A	INTRINSIC
low complexity region	1642	1671	N/A	INTRINSIC
low complexity region	1747	1758	N/A	INTRINSIC
low complexity region	1810	1823	N/A	INTRINSIC
low complexity region	1888	1903	N/A	INTRINSIC
low complexity region	1940	1955	N/A	INTRINSIC
low complexity region	2003	2012	N/A	INTRINSIC
internal_repeat_2	2015	2115	6.27e-5	PROSPERO
low complexity region	2127	2147	N/A	INTRINSIC
low complexity region	2169	2191	N/A	INTRINSIC
low complexity region	2207	2219	N/A	INTRINSIC
low complexity region	2304	2323	N/A	INTRINSIC
low complexity region	2332	2371	N/A	INTRINSIC
low complexity region	2396	2413	N/A	INTRINSIC
low complexity region	2518	2533	N/A	INTRINSIC
low complexity region	2545	2555	N/A	INTRINSIC
low complexity region	2696	2722	N/A	INTRINSIC
low complexity region	2931	2942	N/A	INTRINSIC
low complexity region	2994	3006	N/A	INTRINSIC
low complexity region	3192	3212	N/A	INTRINSIC
low complexity region	3299	3337	N/A	INTRINSIC
Pfam:SPOC	3465	3586	2.7e-29	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000105786
AA Change: T2500I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000101412
Gene: ENSMUSG00000040761
AA Change: T2500I

Domain	Start	End	E-Value	Type
RRM	7	77	7.77e-12	SMART
low complexity region	109	121	N/A	INTRINSIC
low complexity region	235	257	N/A	INTRINSIC
low complexity region	262	311	N/A	INTRINSIC
RRM	338	411	8.6e-5	SMART
RRM	441	511	1.56e-16	SMART
RRM	520	587	1.84e-13	SMART
low complexity region	692	714	N/A	INTRINSIC
low complexity region	718	743	N/A	INTRINSIC
low complexity region	772	796	N/A	INTRINSIC
coiled coil region	823	848	N/A	INTRINSIC
low complexity region	853	864	N/A	INTRINSIC
internal_repeat_2	867	977	8.58e-5	PROSPERO
coiled coil region	1517	1545	N/A	INTRINSIC
low complexity region	1610	1650	N/A	INTRINSIC
low complexity region	1658	1664	N/A	INTRINSIC
low complexity region	1665	1694	N/A	INTRINSIC
low complexity region	1770	1781	N/A	INTRINSIC
low complexity region	1833	1846	N/A	INTRINSIC
low complexity region	1911	1926	N/A	INTRINSIC
low complexity region	1963	1978	N/A	INTRINSIC
low complexity region	2026	2035	N/A	INTRINSIC
internal_repeat_2	2038	2138	8.58e-5	PROSPERO
low complexity region	2150	2170	N/A	INTRINSIC
low complexity region	2192	2214	N/A	INTRINSIC
low complexity region	2230	2242	N/A	INTRINSIC
low complexity region	2327	2346	N/A	INTRINSIC
low complexity region	2355	2394	N/A	INTRINSIC
low complexity region	2419	2436	N/A	INTRINSIC
low complexity region	2541	2556	N/A	INTRINSIC
low complexity region	2568	2578	N/A	INTRINSIC
low complexity region	2719	2745	N/A	INTRINSIC
low complexity region	2954	2965	N/A	INTRINSIC
low complexity region	3017	3029	N/A	INTRINSIC
low complexity region	3215	3235	N/A	INTRINSIC
low complexity region	3322	3360	N/A	INTRINSIC
Pfam:SPOC	3488	3609	2.7e-29	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147227

Meta Mutation Damage Score

0.1547

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

97% (70/72)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice die during late gestation exhibiting morphological abnormalities of the heart, pancreas, and liver. Inactivation of this gene also affects the differentiation of B cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008E08Rik	A	T	16: 90,554,210 (GRCm38)		noncoding transcript	Het
2700049A03Rik	C	A	12: 71,188,791 (GRCm38)	P1172T	probably benign	Het
Abi3bp	T	A	16: 56,642,475 (GRCm38)		probably null	Het
Apod	T	A	16: 31,311,066 (GRCm38)	H24L	probably damaging	Het
Arfgef3	T	C	10: 18,600,460 (GRCm38)	E1537G	probably damaging	Het
B020004C17Rik	T	C	14: 57,016,579 (GRCm38)	V53A	possibly damaging	Het
Ccdc113	C	A	8: 95,540,796 (GRCm38)		probably null	Het
Cd7	T	G	11: 121,038,110 (GRCm38)	D105A	probably damaging	Het
Celsr3	C	T	9: 108,843,158 (GRCm38)	T2550M	probably benign	Het
Cibar1	T	C	4: 12,171,195 (GRCm38)	Q86R	probably benign	Het
Col6a5	C	T	9: 105,946,083 (GRCm38)	G25D	unknown	Het
Cps1	A	G	1: 67,172,709 (GRCm38)	M679V	probably benign	Het
Dnai4	A	T	4: 103,049,533 (GRCm38)	D694E	probably benign	Het
Dync1h1	A	T	12: 110,615,068 (GRCm38)	T316S	probably benign	Het
Edrf1	T	C	7: 133,650,566 (GRCm38)	Y449H	probably damaging	Het
Fkbpl	G	A	17: 34,645,329 (GRCm38)	A24T	probably benign	Het
Flg2	A	T	3: 93,220,566 (GRCm38)	I2262L	unknown	Het
Flnc	T	C	6: 29,457,554 (GRCm38)	L2417P	probably damaging	Het
Gabrp	A	T	11: 33,567,310 (GRCm38)	Y121N	probably damaging	Het
Gdpd4	A	G	7: 97,966,336 (GRCm38)	T123A	possibly damaging	Het
Gpr162	C	A	6: 124,861,269 (GRCm38)	M139I	probably benign	Het
Greb1l	G	A	18: 10,542,427 (GRCm38)	E1341K	probably damaging	Het
Gsn	G	A	2: 35,296,472 (GRCm38)	S410N	probably benign	Het
Gtf2f2	T	C	14: 75,897,649 (GRCm38)	Y212C	probably damaging	Het
Hao2	C	T	3: 98,877,177 (GRCm38)	A291T	probably damaging	Het
Igkv1-133	A	G	6: 67,725,579 (GRCm38)	T94A	possibly damaging	Het
Irf2bp1	G	A	7: 19,004,998 (GRCm38)	A188T	possibly damaging	Het
Itpr1	T	C	6: 108,406,145 (GRCm38)	V1478A	possibly damaging	Het
Kdm5b	T	C	1: 134,622,099 (GRCm38)		probably null	Het
Kif5b	A	T	18: 6,234,882 (GRCm38)	D49E	probably damaging	Het
Lmbr1l	A	G	15: 98,912,242 (GRCm38)	W113R	probably damaging	Het
Lrp2	T	C	2: 69,513,354 (GRCm38)	D887G	probably damaging	Het
Lrrc14	T	A	15: 76,713,943 (GRCm38)	M291K	probably benign	Het
Lypd11	T	C	7: 24,723,411 (GRCm38)	E79G	probably benign	Het
Mgarp	G	A	3: 51,388,966 (GRCm38)	A205V	possibly damaging	Het
Msl2	T	C	9: 101,101,407 (GRCm38)		probably null	Het
Nfix	G	A	8: 84,713,777 (GRCm38)	Q487*	probably null	Het
Notch4	T	C	17: 34,565,289 (GRCm38)	V22A	probably benign	Het
Npc1l1	T	A	11: 6,222,221 (GRCm38)	Q823L	probably benign	Het
Obox3	T	C	7: 15,626,849 (GRCm38)	K122E	probably benign	Het
Or10ab5	C	T	7: 108,646,548 (GRCm38)	M9I	probably benign	Het
Or2a12	A	G	6: 42,928,038 (GRCm38)	K269R	probably damaging	Het
Or8s10	T	C	15: 98,438,332 (GRCm38)	Y288H	probably damaging	Het
Plekhh3	T	A	11: 101,166,571 (GRCm38)	M287L	possibly damaging	Het
Prpf3	A	T	3: 95,853,545 (GRCm38)	I15K	probably benign	Het
Rpl12-ps1	G	T	1: 36,958,342 (GRCm38)		noncoding transcript	Het
Rps8	G	C	4: 117,155,155 (GRCm38)		probably benign	Het
Setd2	T	C	9: 110,617,831 (GRCm38)	V2489A	probably damaging	Het
Slfn10-ps	T	A	11: 83,029,025 (GRCm38)		noncoding transcript	Het
Smad2	C	T	18: 76,262,724 (GRCm38)	P78L	probably damaging	Het
Stmnd1	A	G	13: 46,299,598 (GRCm38)	D250G	probably benign	Het
Tjp2	T	G	19: 24,131,204 (GRCm38)	E181D	probably benign	Het
Tmem131l	T	C	3: 83,899,265 (GRCm38)	D1478G	probably benign	Het
Trim72	G	T	7: 128,010,004 (GRCm38)	R326L	probably benign	Het
Ttn	A	T	2: 76,897,240 (GRCm38)		probably benign	Het
Vmn1r177	A	T	7: 23,866,073 (GRCm38)	M126K	probably damaging	Het
Vmn1r57	A	G	7: 5,221,320 (GRCm38)	I281M	probably damaging	Het
Vmn2r108	C	A	17: 20,471,403 (GRCm38)	R286L	probably benign	Het
Wdr25	T	C	12: 108,898,042 (GRCm38)	S38P	probably benign	Het
Zfp937	T	A	2: 150,238,309 (GRCm38)	Y86*	probably null	Het
Zfr2	CTCAGACTGGTGTCAGAC	CTCAGAC	10: 81,246,710 (GRCm38)		probably null	Het
Zswim1	C	T	2: 164,825,925 (GRCm38)	H366Y	probably damaging	Het

Other mutations in Spen

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01133:Spen	APN	4	141,489,901 (GRCm38)	missense	unknown
IGL01357:Spen	APN	4	141,517,113 (GRCm38)	missense	unknown
IGL02184:Spen	APN	4	141,487,606 (GRCm38)	missense	unknown
IGL02226:Spen	APN	4	141,478,146 (GRCm38)	missense	unknown
IGL02321:Spen	APN	4	141,517,130 (GRCm38)	missense	unknown
IGL02350:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02357:Spen	APN	4	141,477,579 (GRCm38)	missense	unknown
IGL02627:Spen	APN	4	141,473,015 (GRCm38)	missense	probably damaging	0.99
IGL02683:Spen	APN	4	141,471,645 (GRCm38)	missense	probably benign	0.06
IGL02945:Spen	APN	4	141,494,313 (GRCm38)	missense	unknown
IGL02950:Spen	APN	4	141,469,508 (GRCm38)	missense	probably damaging	1.00
IGL03008:Spen	APN	4	141,476,137 (GRCm38)	missense	possibly damaging	0.70
IGL03019:Spen	APN	4	141,478,916 (GRCm38)	missense	unknown
IGL03038:Spen	APN	4	141,538,239 (GRCm38)	missense	unknown
IGL03334:Spen	APN	4	141,469,969 (GRCm38)	missense	probably damaging	1.00
filtered	UTSW	4	141,477,372 (GRCm38)	missense	unknown
mentholated	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R0105:Spen	UTSW	4	141,469,810 (GRCm38)	splice site	probably benign
R0268:Spen	UTSW	4	141,477,557 (GRCm38)	missense	unknown
R0359:Spen	UTSW	4	141,516,870 (GRCm38)	missense	unknown
R0394:Spen	UTSW	4	141,474,203 (GRCm38)	missense	probably benign	0.03
R0423:Spen	UTSW	4	141,479,336 (GRCm38)	missense	unknown
R0433:Spen	UTSW	4	141,483,758 (GRCm38)	missense	unknown
R0462:Spen	UTSW	4	141,473,651 (GRCm38)	missense	probably damaging	1.00
R0687:Spen	UTSW	4	141,488,028 (GRCm38)	missense	unknown
R0699:Spen	UTSW	4	141,474,391 (GRCm38)	missense	possibly damaging	0.72
R0865:Spen	UTSW	4	141,471,870 (GRCm38)	missense	probably benign	0.11
R0918:Spen	UTSW	4	141,485,564 (GRCm38)	missense	unknown
R1034:Spen	UTSW	4	141,475,752 (GRCm38)	missense	probably benign	0.33
R1341:Spen	UTSW	4	141,469,400 (GRCm38)	missense	possibly damaging	0.78
R1401:Spen	UTSW	4	141,471,821 (GRCm38)	missense	probably damaging	0.98
R1509:Spen	UTSW	4	141,475,635 (GRCm38)	missense	probably benign	0.00
R1509:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R1561:Spen	UTSW	4	141,472,383 (GRCm38)	nonsense	probably null
R1589:Spen	UTSW	4	141,488,024 (GRCm38)	missense	unknown
R1640:Spen	UTSW	4	141,468,943 (GRCm38)	missense	probably damaging	0.98
R1758:Spen	UTSW	4	141,476,375 (GRCm38)	missense	unknown
R1764:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R1824:Spen	UTSW	4	141,472,785 (GRCm38)	missense	probably damaging	1.00
R1899:Spen	UTSW	4	141,470,343 (GRCm38)	missense	probably benign	0.17
R1916:Spen	UTSW	4	141,472,598 (GRCm38)	missense	probably damaging	1.00
R2011:Spen	UTSW	4	141,473,329 (GRCm38)	missense	probably damaging	1.00
R2295:Spen	UTSW	4	141,477,273 (GRCm38)	missense	unknown
R2379:Spen	UTSW	4	141,516,927 (GRCm38)	missense	unknown
R2404:Spen	UTSW	4	141,477,905 (GRCm38)	missense	unknown
R3719:Spen	UTSW	4	141,517,183 (GRCm38)	missense	unknown
R3889:Spen	UTSW	4	141,477,881 (GRCm38)	missense	unknown
R3945:Spen	UTSW	4	141,477,353 (GRCm38)	missense	unknown
R4227:Spen	UTSW	4	141,522,147 (GRCm38)	missense	unknown
R4326:Spen	UTSW	4	141,477,372 (GRCm38)	missense	unknown
R4382:Spen	UTSW	4	141,473,139 (GRCm38)	missense	possibly damaging	0.88
R4542:Spen	UTSW	4	141,476,786 (GRCm38)	missense	unknown
R4757:Spen	UTSW	4	141,473,079 (GRCm38)	nonsense	probably null
R4771:Spen	UTSW	4	141,472,596 (GRCm38)	missense	probably benign	0.14
R5072:Spen	UTSW	4	141,522,302 (GRCm38)	missense	unknown
R5121:Spen	UTSW	4	141,476,099 (GRCm38)	missense	probably benign	0.00
R5176:Spen	UTSW	4	141,476,276 (GRCm38)	missense	unknown
R5291:Spen	UTSW	4	141,488,079 (GRCm38)	missense	unknown
R5293:Spen	UTSW	4	141,472,406 (GRCm38)	missense	possibly damaging	0.89
R5347:Spen	UTSW	4	141,471,485 (GRCm38)	missense	probably benign	0.26
R5511:Spen	UTSW	4	141,516,838 (GRCm38)	missense	unknown
R5511:Spen	UTSW	4	141,475,064 (GRCm38)	missense	possibly damaging	0.86
R5772:Spen	UTSW	4	141,478,184 (GRCm38)	missense	unknown
R5834:Spen	UTSW	4	141,471,843 (GRCm38)	missense	possibly damaging	0.63
R5858:Spen	UTSW	4	141,473,871 (GRCm38)	missense	probably benign	0.05
R6214:Spen	UTSW	4	141,479,112 (GRCm38)	missense	unknown
R6232:Spen	UTSW	4	141,517,022 (GRCm38)	missense	unknown
R6345:Spen	UTSW	4	141,471,633 (GRCm38)	missense	possibly damaging	0.86
R6419:Spen	UTSW	4	141,476,310 (GRCm38)	missense	unknown
R6455:Spen	UTSW	4	141,475,509 (GRCm38)	missense	probably damaging	0.97
R6979:Spen	UTSW	4	141,478,063 (GRCm38)	missense	unknown
R6994:Spen	UTSW	4	141,493,459 (GRCm38)	missense	unknown
R7018:Spen	UTSW	4	141,493,444 (GRCm38)	missense	unknown
R7040:Spen	UTSW	4	141,494,382 (GRCm38)	missense	unknown
R7127:Spen	UTSW	4	141,476,108 (GRCm38)	missense	possibly damaging	0.53
R7218:Spen	UTSW	4	141,472,650 (GRCm38)	missense	possibly damaging	0.54
R7234:Spen	UTSW	4	141,479,135 (GRCm38)	missense	unknown
R7316:Spen	UTSW	4	141,477,054 (GRCm38)	missense	unknown
R7350:Spen	UTSW	4	141,479,385 (GRCm38)	missense	unknown
R7356:Spen	UTSW	4	141,471,924 (GRCm38)	nonsense	probably null
R7400:Spen	UTSW	4	141,473,741 (GRCm38)	missense	probably damaging	1.00
R7470:Spen	UTSW	4	141,479,294 (GRCm38)	missense	unknown
R7698:Spen	UTSW	4	141,472,845 (GRCm38)	missense	probably damaging	1.00
R7858:Spen	UTSW	4	141,488,131 (GRCm38)	splice site	probably null
R8033:Spen	UTSW	4	141,471,746 (GRCm38)	missense	probably benign	0.03
R8064:Spen	UTSW	4	141,475,700 (GRCm38)	missense	possibly damaging	0.53
R8159:Spen	UTSW	4	141,475,003 (GRCm38)	missense	possibly damaging	0.53
R8187:Spen	UTSW	4	141,472,905 (GRCm38)	missense	possibly damaging	0.93
R8463:Spen	UTSW	4	141,522,279 (GRCm38)	missense	unknown
R8557:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8558:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8672:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8673:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8674:Spen	UTSW	4	141,470,370 (GRCm38)	missense	probably benign	0.14
R8714:Spen	UTSW	4	141,488,003 (GRCm38)	missense	unknown
R8735:Spen	UTSW	4	141,469,818 (GRCm38)	missense	probably benign	0.32
R8762:Spen	UTSW	4	141,472,950 (GRCm38)	missense	probably damaging	1.00
R8877:Spen	UTSW	4	141,471,826 (GRCm38)	nonsense	probably null
R8878:Spen	UTSW	4	141,477,209 (GRCm38)	missense	unknown
R8937:Spen	UTSW	4	141,474,063 (GRCm38)	missense	probably damaging	1.00
R8939:Spen	UTSW	4	141,475,658 (GRCm38)	missense	possibly damaging	0.72
R8968:Spen	UTSW	4	141,470,390 (GRCm38)	missense	probably benign	0.02
R8971:Spen	UTSW	4	141,474,578 (GRCm38)	missense	possibly damaging	0.53
R9016:Spen	UTSW	4	141,473,627 (GRCm38)	missense	probably damaging	1.00
R9072:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9073:Spen	UTSW	4	141,476,391 (GRCm38)	missense	unknown
R9120:Spen	UTSW	4	141,472,922 (GRCm38)	missense
R9136:Spen	UTSW	4	141,522,312 (GRCm38)	missense	unknown
R9138:Spen	UTSW	4	141,469,486 (GRCm38)	missense	probably damaging	1.00
R9150:Spen	UTSW	4	141,517,157 (GRCm38)	missense	unknown
R9225:Spen	UTSW	4	141,475,632 (GRCm38)	missense	possibly damaging	0.53
R9492:Spen	UTSW	4	141,471,787 (GRCm38)	missense	probably benign	0.26
R9537:Spen	UTSW	4	141,516,845 (GRCm38)	small deletion	probably benign
R9537:Spen	UTSW	4	141,471,704 (GRCm38)	missense	probably benign	0.15
R9602:Spen	UTSW	4	141,477,872 (GRCm38)	missense	unknown
R9609:Spen	UTSW	4	141,488,108 (GRCm38)	missense	unknown
R9686:Spen	UTSW	4	141,472,635 (GRCm38)	missense	probably benign	0.27
R9697:Spen	UTSW	4	141,468,964 (GRCm38)	missense	probably damaging	1.00
R9713:Spen	UTSW	4	141,517,020 (GRCm38)	missense	unknown
T0722:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
T0975:Spen	UTSW	4	141,474,353 (GRCm38)	missense	probably benign	0.33
Z1088:Spen	UTSW	4	141,477,976 (GRCm38)	missense	unknown
Z1088:Spen	UTSW	4	141,477,977 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- GAGGATGCTTAGGGTCACAG -3'
(R):5'- CAGGTTCAAGGTGCACTCGATC -3'

Sequencing Primer
(F):5'- ACTCACGGGCTCTGCAATG -3'
(R):5'- ATCATCGAGAGTGACCCTGTGAC -3'

Posted On

2016-08-04