Incidental Mutation 'R5382:Prr23a2'
ID424793
Institutional Source Beutler Lab
Gene Symbol Prr23a2
Ensembl Gene ENSMUSG00000063058
Gene Nameproline rich 23A, member 2
SynonymsGm6406
MMRRC Submission 042957-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.058) question?
Stock #R5382 (G1)
Quality Score225
Status Validated
Chromosome9
Chromosomal Location98856494-98857374 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 98857176 bp
ZygosityHeterozygous
Amino Acid Change Tyrosine to Asparagine at position 196 (Y196N)
Ref Sequence ENSEMBL: ENSMUSP00000071270 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000071302]
Predicted Effect probably damaging
Transcript: ENSMUST00000071302
AA Change: Y196N

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000071270
Gene: ENSMUSG00000063058
AA Change: Y196N

DomainStartEndE-ValueType
Pfam:DUF2476 2 250 1.5e-92 PFAM
Meta Mutation Damage Score 0.6467 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.4%
  • 20x: 95.7%
Validation Efficiency 100% (59/59)
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2810474O19Rik G T 6: 149,326,460 E335* probably null Het
Acp7 G A 7: 28,615,419 P250S possibly damaging Het
Actn2 T A 13: 12,308,951 M133L probably benign Het
AI314180 A T 4: 58,850,934 M413K probably benign Het
Arhgap32 A T 9: 32,152,010 K105M probably damaging Het
BC034090 A G 1: 155,225,603 V305A probably benign Het
Brd1 G A 15: 88,729,564 T376M probably damaging Het
Cbl C T 9: 44,159,021 A505T probably benign Het
Cga G T 4: 34,904,048 M14I probably benign Het
Cluh T C 11: 74,665,109 probably benign Het
Col14a1 A T 15: 55,362,436 D165V unknown Het
Cp T C 3: 19,978,925 W639R probably damaging Het
Cyp7b1 T A 3: 18,097,221 D276V possibly damaging Het
Dctd C T 8: 48,137,414 probably benign Het
Erc1 T A 6: 119,761,272 M509L probably benign Het
Evi5l A G 8: 4,178,653 probably benign Het
Exoc6 T C 19: 37,598,679 probably null Het
Gm38706 C T 6: 130,483,781 noncoding transcript Het
Gpr183 T C 14: 121,954,921 T63A possibly damaging Het
Gpr63 T A 4: 25,007,952 D225E probably benign Het
Grb14 T A 2: 64,914,734 K93N probably damaging Het
Igkv4-80 A C 6: 69,016,665 S81A probably benign Het
Kif28 C T 1: 179,700,282 G768D probably damaging Het
Krt79 A T 15: 101,931,440 D373E probably benign Het
Mro G A 18: 73,876,822 S187N probably benign Het
Ms4a14 G T 19: 11,303,057 D712E possibly damaging Het
Narfl A G 17: 25,776,920 probably benign Het
Ndufaf1 T C 2: 119,660,412 T56A possibly damaging Het
Nell2 A T 15: 95,229,210 D761E probably damaging Het
Numb A G 12: 83,808,205 F116L probably damaging Het
Olfr1016 T C 2: 85,800,148 N41D probably damaging Het
Olfr1065 A G 2: 86,445,316 L222P probably damaging Het
Olfr1219 A G 2: 89,074,735 Y119H probably damaging Het
Olfr235 A T 19: 12,268,409 M60L possibly damaging Het
Olfr410 T A 11: 74,334,980 M84L probably benign Het
Olfr798 T A 10: 129,626,007 D18V probably damaging Het
Otog A C 7: 46,249,004 N182T probably damaging Het
Otx1 C A 11: 21,997,037 A91S probably damaging Het
Padi6 A T 4: 140,731,210 V457E probably damaging Het
Pex16 G A 2: 92,377,530 R109H possibly damaging Het
Phactr1 T C 13: 43,135,219 probably benign Het
Phf20 G A 2: 156,267,497 E255K probably damaging Het
Pim1 A G 17: 29,491,483 probably benign Het
Prune2 A T 19: 17,003,659 N60I probably damaging Het
Ptprb T A 10: 116,353,871 Y1812N probably damaging Het
Rab11fip4 A G 11: 79,690,715 Y512C possibly damaging Het
Rft1 T C 14: 30,666,782 V221A probably benign Het
Tacr2 T C 10: 62,261,497 M252T probably damaging Het
Tgfbrap1 A G 1: 43,075,865 I25T probably benign Het
Th A G 7: 142,895,440 F191S probably damaging Het
Trim3 C A 7: 105,618,347 R275L probably benign Het
Trpm3 A G 19: 22,885,341 probably null Het
Wars A T 12: 108,882,780 D80E probably benign Het
Wdr90 T A 17: 25,845,598 Y1806F probably damaging Het
Zfp644 A T 5: 106,634,869 I1182N possibly damaging Het
Other mutations in Prr23a2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00970:Prr23a2 APN 9 98856961 missense probably benign 0.03
IGL01867:Prr23a2 APN 9 98857060 missense probably benign
R0542:Prr23a2 UTSW 9 98857033 missense probably benign 0.00
R0566:Prr23a2 UTSW 9 98856988 missense possibly damaging 0.95
R0831:Prr23a2 UTSW 9 98856864 missense probably damaging 1.00
R7309:Prr23a2 UTSW 9 98856974 missense probably benign 0.43
Predicted Primers PCR Primer
(F):5'- CCAGGATCATCTAGTCGGTCTC -3'
(R):5'- ATTTGTGCAGGGCAGTTCAGTC -3'

Sequencing Primer
(F):5'- GAGGAAACCTGTGAGTACTTCTACC -3'
(R):5'- GGCAGTTCAGTCATAGACCAGTC -3'
Posted On2016-08-04