Mutagenetix > Incidental Mutation

Incidental Mutation 'R5379:Helz2'

425728

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

MMRRC Submission

042954-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5379 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

181227615-181242027 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 181235069 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 1211 (T1211S)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094203
AA Change: T1211S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: T1211S

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108831
AA Change: T1211S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: T1211S

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000121484
AA Change: T1211S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: T1211S

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149417

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155049

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.5%

Validation Efficiency

97% (58/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy1	T	A	11: 7,146,532 (GRCm38)	L640Q	probably damaging	Het
Afap1l1	T	C	18: 61,758,650 (GRCm38)	E32G	probably damaging	Het
Atp1a1	T	A	3: 101,582,095 (GRCm38)	M734L	probably benign	Het
B4galt6	T	C	18: 20,689,239 (GRCm38)	D294G	probably damaging	Het
Baz1a	T	G	12: 54,894,348 (GRCm38)	D1539A	probably damaging	Het
Bmper	T	A	9: 23,297,224 (GRCm38)	S141T	probably benign	Het
Camkv	T	C	9: 107,945,346 (GRCm38)	V20A	probably damaging	Het
Chst8	A	G	7: 34,675,854 (GRCm38)	Y187H	probably damaging	Het
Coro1c	A	G	5: 113,845,382 (GRCm38)	Y362H	probably damaging	Het
Csmd3	C	A	15: 47,636,450 (GRCm38)	G3008*	probably null	Het
Dnah17	T	C	11: 118,117,203 (GRCm38)		probably benign	Het
Dnajb1	C	T	8: 83,608,506 (GRCm38)	R59C	possibly damaging	Het
Dpf1	A	G	7: 29,304,108 (GRCm38)	K10E	probably benign	Het
Eif5	T	C	12: 111,543,555 (GRCm38)	L311P	probably damaging	Het
Eqtn	A	G	4: 94,907,588 (GRCm38)	F251S	probably damaging	Het
Fancg	A	G	4: 43,002,998 (GRCm38)	S620P	probably benign	Het
Farp1	T	C	14: 121,256,757 (GRCm38)	V550A	possibly damaging	Het
Fat2	T	C	11: 55,303,941 (GRCm38)	T1091A	probably damaging	Het
Fbxo33	A	G	12: 59,219,460 (GRCm38)		probably benign	Het
Fndc5	A	T	4: 129,142,094 (GRCm38)	I175F	probably damaging	Het
Gtf2ird2	G	C	5: 134,217,468 (GRCm38)	R856P	probably benign	Het
Hc	A	T	2: 34,991,065 (GRCm38)	F1481I	probably damaging	Het
Hmcn2	G	A	2: 31,409,011 (GRCm38)	V2790M	probably damaging	Het
Ighv1-82	T	C	12: 115,952,677 (GRCm38)	Y71C	probably damaging	Het
Itgam	A	G	7: 128,112,388 (GRCm38)	D725G	probably damaging	Het
Kif9	T	C	9: 110,521,303 (GRCm38)	V754A	probably benign	Het
Larp4b	C	A	13: 9,136,909 (GRCm38)	T91K	probably benign	Het
Mki67	T	C	7: 135,697,461 (GRCm38)	E1948G	possibly damaging	Het
Mllt6	G	A	11: 97,669,500 (GRCm38)	S210N	possibly damaging	Het
Mrgprb13	A	T	7: 48,311,748 (GRCm38)		noncoding transcript	Het
Nlrc4	A	T	17: 74,448,083 (GRCm38)	L46*	probably null	Het
Or13c7c	A	G	4: 43,836,010 (GRCm38)	I160T	probably benign	Het
Or7g21	A	T	9: 19,121,077 (GRCm38)	T38S	probably damaging	Het
Or8d1b	T	C	9: 38,975,855 (GRCm38)	Y60H	possibly damaging	Het
Orm1	A	G	4: 63,345,993 (GRCm38)		probably null	Het
Parva	A	T	7: 112,579,720 (GRCm38)	H311L	probably benign	Het
Pramel25	A	G	4: 143,794,923 (GRCm38)	I370V	probably benign	Het
Proca1	C	A	11: 78,205,266 (GRCm38)	S154R	probably damaging	Het
R3hdm2	T	C	10: 127,471,902 (GRCm38)	V344A	probably damaging	Het
Rabep1	A	T	11: 70,908,421 (GRCm38)	K293N	probably damaging	Het
Ranbp6	A	G	19: 29,811,683 (GRCm38)	V423A	probably damaging	Het
Rnf20	T	A	4: 49,652,639 (GRCm38)	Y711N	possibly damaging	Het
Sf3b1	A	T	1: 55,003,150 (GRCm38)	M498K	possibly damaging	Het
Sin3a	T	C	9: 57,110,988 (GRCm38)	M897T	probably benign	Het
Sp140	T	A	1: 85,610,828 (GRCm38)	D95E	possibly damaging	Het
Srbd1	A	C	17: 86,001,536 (GRCm38)	I738S	possibly damaging	Het
Srsf11	C	T	3: 158,023,344 (GRCm38)		probably benign	Het
Svep1	A	G	4: 58,072,991 (GRCm38)	V2106A	possibly damaging	Het
Tasor2	T	C	13: 3,588,496 (GRCm38)	R412G	probably benign	Het
Tex47	G	A	5: 7,304,843 (GRCm38)	R8Q	probably null	Het
Trpc7	A	G	13: 56,804,550 (GRCm38)	Y548H	probably damaging	Het
Vmn1r170	A	T	7: 23,606,629 (GRCm38)	H152L	possibly damaging	Het
Zfp184	A	G	13: 21,959,881 (GRCm38)	I586V	probably damaging	Het
Zfp454	T	C	11: 50,883,802 (GRCm38)	T15A	probably damaging	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	181,229,702 (GRCm38)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	181,233,006 (GRCm38)	nonsense	probably null
IGL00704:Helz2	APN	2	181,234,385 (GRCm38)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	181,232,245 (GRCm38)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	181,233,977 (GRCm38)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	181,232,881 (GRCm38)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	181,238,481 (GRCm38)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	181,232,185 (GRCm38)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	181,231,690 (GRCm38)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	181,230,911 (GRCm38)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	181,231,022 (GRCm38)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	181,235,026 (GRCm38)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	181,231,146 (GRCm38)	intron	probably benign
IGL03003:Helz2	APN	2	181,240,253 (GRCm38)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	181,229,222 (GRCm38)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	181,231,804 (GRCm38)	missense	probably benign	0.00
Colby	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	181,234,834 (GRCm38)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	181,240,959 (GRCm38)	missense	probably benign
R0013:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	181,240,511 (GRCm38)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	181,228,821 (GRCm38)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	181,236,407 (GRCm38)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	181,237,802 (GRCm38)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	181,232,269 (GRCm38)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	181,230,430 (GRCm38)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	181,232,759 (GRCm38)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	181,230,593 (GRCm38)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	181,232,209 (GRCm38)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	181,229,656 (GRCm38)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	181,227,770 (GRCm38)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	181,234,825 (GRCm38)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	181,232,089 (GRCm38)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	181,232,116 (GRCm38)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	181,240,881 (GRCm38)	missense	probably benign
R0826:Helz2	UTSW	2	181,240,853 (GRCm38)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	181,230,777 (GRCm38)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	181,236,135 (GRCm38)	missense	probably benign
R1170:Helz2	UTSW	2	181,229,815 (GRCm38)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	181,231,128 (GRCm38)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	181,237,596 (GRCm38)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	181,232,981 (GRCm38)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	181,235,524 (GRCm38)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	181,239,654 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	181,232,804 (GRCm38)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	181,233,228 (GRCm38)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	181,236,297 (GRCm38)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	181,234,147 (GRCm38)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	181,236,263 (GRCm38)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	181,238,459 (GRCm38)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	181,234,987 (GRCm38)	missense	probably benign
R1837:Helz2	UTSW	2	181,229,289 (GRCm38)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	181,232,085 (GRCm38)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	181,234,289 (GRCm38)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	181,233,750 (GRCm38)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	181,231,329 (GRCm38)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	181,232,578 (GRCm38)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	181,237,479 (GRCm38)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	181,240,544 (GRCm38)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	181,235,102 (GRCm38)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	181,231,380 (GRCm38)	missense	probably benign
R2180:Helz2	UTSW	2	181,233,732 (GRCm38)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	181,229,048 (GRCm38)	nonsense	probably null
R2248:Helz2	UTSW	2	181,233,433 (GRCm38)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	181,232,912 (GRCm38)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	181,240,742 (GRCm38)	missense	probably benign
R3617:Helz2	UTSW	2	181,233,061 (GRCm38)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	181,240,389 (GRCm38)	nonsense	probably null
R3803:Helz2	UTSW	2	181,239,996 (GRCm38)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	181,229,710 (GRCm38)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	181,240,475 (GRCm38)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	181,229,902 (GRCm38)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	181,228,833 (GRCm38)	missense	probably benign
R4624:Helz2	UTSW	2	181,239,308 (GRCm38)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	181,238,417 (GRCm38)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	181,237,417 (GRCm38)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	181,230,120 (GRCm38)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	181,236,147 (GRCm38)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	181,232,438 (GRCm38)	missense	possibly damaging	0.80
R4965:Helz2	UTSW	2	181,240,916 (GRCm38)	missense	possibly damaging	0.79
R5022:Helz2	UTSW	2	181,240,569 (GRCm38)	missense	probably benign
R5089:Helz2	UTSW	2	181,235,149 (GRCm38)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	181,230,757 (GRCm38)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	181,234,846 (GRCm38)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	181,235,528 (GRCm38)	missense	probably damaging	1.00
R5559:Helz2	UTSW	2	181,230,126 (GRCm38)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	181,240,258 (GRCm38)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	181,237,289 (GRCm38)	intron	probably benign
R5805:Helz2	UTSW	2	181,240,508 (GRCm38)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	181,236,396 (GRCm38)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	181,232,656 (GRCm38)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	181,234,028 (GRCm38)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	181,230,384 (GRCm38)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	181,230,767 (GRCm38)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	181,231,050 (GRCm38)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	181,240,313 (GRCm38)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	181,233,038 (GRCm38)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	181,232,294 (GRCm38)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	181,235,945 (GRCm38)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	181,233,202 (GRCm38)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	181,233,467 (GRCm38)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	181,235,069 (GRCm38)	missense	probably benign
R6581:Helz2	UTSW	2	181,229,379 (GRCm38)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	181,239,557 (GRCm38)	nonsense	probably null
R6964:Helz2	UTSW	2	181,230,428 (GRCm38)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	181,240,514 (GRCm38)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	181,231,285 (GRCm38)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	181,238,423 (GRCm38)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	181,235,600 (GRCm38)	splice site	probably null
R7512:Helz2	UTSW	2	181,230,854 (GRCm38)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	181,237,572 (GRCm38)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	181,231,996 (GRCm38)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	181,230,355 (GRCm38)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	181,234,531 (GRCm38)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	181,233,991 (GRCm38)	missense	probably benign
R7799:Helz2	UTSW	2	181,237,989 (GRCm38)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	181,232,902 (GRCm38)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	181,237,750 (GRCm38)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	181,240,205 (GRCm38)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	181,237,896 (GRCm38)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	181,238,262 (GRCm38)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	181,238,102 (GRCm38)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	181,230,157 (GRCm38)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	181,229,331 (GRCm38)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	181,229,557 (GRCm38)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	181,232,767 (GRCm38)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	181,233,127 (GRCm38)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	181,238,380 (GRCm38)	missense
R8953:Helz2	UTSW	2	181,233,091 (GRCm38)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	181,229,614 (GRCm38)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	181,237,788 (GRCm38)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	181,234,693 (GRCm38)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	181,228,999 (GRCm38)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	181,232,468 (GRCm38)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	181,240,175 (GRCm38)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	181,239,640 (GRCm38)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	181,240,055 (GRCm38)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	181,230,090 (GRCm38)	missense	probably benign
R9186:Helz2	UTSW	2	181,234,664 (GRCm38)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	181,240,948 (GRCm38)	missense	probably benign
R9407:Helz2	UTSW	2	181,240,182 (GRCm38)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	181,232,917 (GRCm38)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	181,236,452 (GRCm38)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	181,240,221 (GRCm38)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	181,240,677 (GRCm38)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	181,240,232 (GRCm38)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	181,240,823 (GRCm38)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	181,231,741 (GRCm38)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	181,237,564 (GRCm38)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	181,235,961 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCTCTAGAGGCTGAGTTCCC -3'
(R):5'- CTTCACTGAGCCTCGAGTACTG -3'

Sequencing Primer
(F):5'- CTAGAGGCTGAGTTCCCGCTTG -3'
(R):5'- CTCGAGTACTGAACACGGTCATG -3'

Posted On

2016-08-04