Mutagenetix > Incidental Mutations

Incidental Mutation 'R5394:Myo18a'

426069

Institutional Source

Beutler Lab

Gene Symbol

Myo18a

Ensembl Gene

ENSMUSG00000000631

Gene Name

myosin XVIIIA

Synonyms

MyoPDZ

MMRRC Submission

042966-MU

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R5394 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

77763246-77865980 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 77853350 bp

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 1846 (M1846T)

Ref Sequence

ENSEMBL: ENSMUSP00000104013 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000645] [ENSMUST00000092884] [ENSMUST00000092887] [ENSMUST00000100794] [ENSMUST00000102488] [ENSMUST00000108375] [ENSMUST00000108376] [ENSMUST00000130305] [ENSMUST00000130627] [ENSMUST00000167856] [ENSMUST00000168348] [ENSMUST00000169105] [ENSMUST00000172303] [ENSMUST00000164334]

Predicted Effect

probably benign
Transcript: ENSMUST00000000645
AA Change: M1884T

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000000645
Gene: ENSMUSG00000000631
AA Change: M1884T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	399	1183	1.53e-45	SMART
IQ	1184	1206	1.11e-3	SMART
Pfam:Myosin_tail_1	1219	1867	1.7e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092884
AA Change: M1552T

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000090560
Gene: ENSMUSG00000000631
AA Change: M1552T

Domain	Start	End	E-Value	Type
MYSc	68	851	4.16e-47	SMART
IQ	852	874	1.11e-3	SMART
Pfam:Myosin_tail_1	888	1534	2e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000092887
AA Change: M1883T

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000090563
Gene: ENSMUSG00000000631
AA Change: M1883T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	399	1182	4.16e-47	SMART
IQ	1183	1205	1.11e-3	SMART
Pfam:Myosin_tail_1	1218	1866	3e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000100794
AA Change: M1548T

PolyPhen 2 Score 0.049 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000098358
Gene: ENSMUSG00000000631
AA Change: M1548T

Domain	Start	End	E-Value	Type
MYSc	68	847	1.45e-46	SMART
IQ	848	870	1.11e-3	SMART
Pfam:Myosin_tail_1	884	1530	4.9e-35	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000102488
AA Change: M1883T

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000099546
Gene: ENSMUSG00000000631
AA Change: M1883T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	399	1182	4.16e-47	SMART
IQ	1183	1205	1.11e-3	SMART
Pfam:Myosin_tail_1	1218	1866	3e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108375
AA Change: M1883T

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000104012
Gene: ENSMUSG00000000631
AA Change: M1883T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	399	1182	4.16e-47	SMART
IQ	1183	1205	1.11e-3	SMART
Pfam:Myosin_tail_1	1218	1838	6.8e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108376
AA Change: M1846T

PolyPhen 2 Score 0.137 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000104013
Gene: ENSMUSG00000000631
AA Change: M1846T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	399	1182	4.16e-47	SMART
IQ	1183	1205	1.11e-3	SMART
Blast:MYSc	1258	1387	1e-14	BLAST
low complexity region	1396	1407	N/A	INTRINSIC
low complexity region	1743	1762	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000130305
AA Change: M1564T

PolyPhen 2 Score 0.038 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000119574
Gene: ENSMUSG00000000631
AA Change: M1564T

Domain	Start	End	E-Value	Type
MYSc	80	863	4.16e-47	SMART
IQ	864	886	1.11e-3	SMART
Pfam:Myosin_tail_1	902	1547	2.6e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000130627
AA Change: M1895T

PolyPhen 2 Score 0.047 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000119839
Gene: ENSMUSG00000000631
AA Change: M1895T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	411	1194	4.16e-47	SMART
IQ	1195	1217	1.11e-3	SMART
Pfam:Myosin_tail_1	1230	1850	6.9e-36	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130956

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135045

Predicted Effect

probably benign
Transcript: ENSMUST00000135375

SMART Domains

Protein: ENSMUSP00000117044
Gene: ENSMUSG00000000631

Domain	Start	End	E-Value	Type
low complexity region	198	231	N/A	INTRINSIC
low complexity region	289	303	N/A	INTRINSIC
low complexity region	361	459	N/A	INTRINSIC
low complexity region	474	486	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000167856
AA Change: M1490T

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000128487
Gene: ENSMUSG00000000631
AA Change: M1490T

Domain	Start	End	E-Value	Type
MYSc	16	789	1.3e-32	SMART
IQ	790	812	1.11e-3	SMART
Blast:MYSc	865	994	1e-14	BLAST
low complexity region	1003	1014	N/A	INTRINSIC
low complexity region	1387	1406	N/A	INTRINSIC
internal_repeat_1	1569	1627	2.13e-5	PROSPERO

Predicted Effect

probably benign
Transcript: ENSMUST00000168348
AA Change: M1931T

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000130696
Gene: ENSMUSG00000000631
AA Change: M1931T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	447	1230	4.16e-47	SMART
IQ	1231	1253	1.11e-3	SMART
Blast:MYSc	1306	1435	1e-14	BLAST
low complexity region	1444	1455	N/A	INTRINSIC
low complexity region	1828	1847	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000169105
AA Change: M1895T

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000132149
Gene: ENSMUSG00000000631
AA Change: M1895T

Domain	Start	End	E-Value	Type
low complexity region	6	27	N/A	INTRINSIC
low complexity region	202	227	N/A	INTRINSIC
PDZ	229	311	5.72e-10	SMART
MYSc	411	1194	4.16e-47	SMART
IQ	1195	1217	1.11e-3	SMART
Pfam:Myosin_tail_1	1230	1878	7.3e-35	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172303
AA Change: M1570T

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000129098
Gene: ENSMUSG00000000631
AA Change: M1570T

Domain	Start	End	E-Value	Type
MYSc	80	863	4.16e-47	SMART
IQ	864	886	1.11e-3	SMART
Pfam:Myosin_tail_1	902	1547	2.6e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000164334
AA Change: M1552T

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000131771
Gene: ENSMUSG00000000631
AA Change: M1552T

Domain	Start	End	E-Value	Type
MYSc	68	851	4.16e-47	SMART
IQ	852	874	1.11e-3	SMART
Pfam:Myosin_tail_1	888	1505	4e-36	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000142571

Meta Mutation Damage Score

0.1355

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

97% (96/99)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene can bind GOLPH3, linking the Golgi to the cytoskeleton and influencing Golgi membrane trafficking. The encoded protein is also part of a complex that assembles lamellar actomyosin bundles and may be required for cell migration. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310050C09Rik	T	C	3: 92,868,698	E226G	probably damaging	Het
4930503B20Rik	A	G	3: 146,650,608	F182L	probably damaging	Het
4930503B20Rik	T	C	3: 146,650,958	Y65C	probably damaging	Het
4930590J08Rik	A	G	6: 91,919,193	T341A	probably benign	Het
4932438A13Rik	G	T	3: 36,917,668	V517F	probably damaging	Het
Adamts13	G	A	2: 26,986,558	V495I	probably benign	Het
Alms1	C	A	6: 85,623,088	T2101K	probably benign	Het
Als2	T	C	1: 59,174,946	D1361G	probably benign	Het
Ankdd1a	A	T	9: 65,505,214	M275K	probably benign	Het
Arfip2	A	T	7: 105,636,976	Y161*	probably null	Het
Asap3	A	T	4: 136,241,259	E707D	probably benign	Het
Atm	C	T	9: 53,507,777		probably null	Het
Atp9b	A	T	18: 80,776,837	S577R	probably benign	Het
Camk1d	G	C	2: 5,303,366	D274E	probably benign	Het
Cebpz	C	T	17: 78,922,205	D907N	probably benign	Het
Cmtm1	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	8: 104,309,470		probably benign	Het
Cnst	T	A	1: 179,601,736		probably benign	Het
Cog2	G	T	8: 124,532,529	V196L	probably benign	Het
Col11a1	A	G	3: 114,194,184		probably null	Het
Cops6	T	A	5: 138,163,500		probably null	Het
Cspg4	A	G	9: 56,890,200	E1316G	probably damaging	Het
Cwc22	G	C	2: 77,929,339	D122E	possibly damaging	Het
Dapk2	G	A	9: 66,268,718	V300M	probably benign	Het
Dcp1b	C	A	6: 119,175,367	D25E	probably damaging	Het
Ddx10	A	T	9: 53,233,857	Y273*	probably null	Het
Dhx58	T	C	11: 100,698,208	E504G	probably benign	Het
Dync2h1	C	T	9: 7,120,899	W2129*	probably null	Het
Egr4	A	T	6: 85,512,460	L206Q	probably damaging	Het
Eif4g3	A	G	4: 138,103,398		probably null	Het
Fbxo46	A	G	7: 19,136,616	N387D	possibly damaging	Het
Fpr-rs3	T	G	17: 20,624,208	M224L	probably benign	Het
Gm14325	G	A	2: 177,832,984	H102Y	possibly damaging	Het
Gxylt2	A	G	6: 100,705,114	K91E	probably benign	Het
H2afy2	G	A	10: 61,751,687	T156M	possibly damaging	Het
Hecw1	A	T	13: 14,322,589	V278E	probably damaging	Het
Hydin	A	G	8: 110,539,842		probably null	Het
Iars	T	C	13: 49,722,165	L776P	probably damaging	Het
Kcnh2	T	A	5: 24,332,041	T182S	probably benign	Het
Lats2	T	G	14: 57,691,353	S1022R	probably benign	Het
Lef1	C	T	3: 131,194,659	P264S	probably damaging	Het
Lyrm1	A	C	7: 119,914,248	I79L	possibly damaging	Het
Man2b2	T	G	5: 36,814,518	Q618P	probably benign	Het
Mei1	T	C	15: 82,092,756	V180A	possibly damaging	Het
Mmp15	A	T	8: 95,366,404	N137I	probably damaging	Het
Mms22l	A	G	4: 24,517,115	D222G	possibly damaging	Het
Mut	T	A	17: 40,947,184	S414T	probably benign	Het
Neo1	A	T	9: 58,990,234	N146K	probably benign	Het
Nos3	A	G	5: 24,383,890	T1174A	probably benign	Het
Olfr1233	A	T	2: 89,339,462	I280N	probably damaging	Het
Olfr417	T	C	1: 174,369,270	Y118H	probably damaging	Het
Olfr566	A	T	7: 102,856,479	S268T	probably damaging	Het
Olfr960	A	T	9: 39,623,134	T4S	probably benign	Het
Pax8	A	T	2: 24,442,910		probably benign	Het
Pde5a	G	A	3: 122,818,009	C532Y	probably damaging	Het
Pigq	T	C	17: 25,931,472	D442G	possibly damaging	Het
Pik3c2g	T	C	6: 139,720,082	V43A	probably benign	Het
Pik3cb	T	C	9: 99,088,663	N325S	probably benign	Het
Pot1b	T	C	17: 55,700,063	K18R	probably benign	Het
Ppp1r42	A	T	1: 9,999,405	L144Q	probably damaging	Het
Prdm13	T	A	4: 21,679,455	Q345L	unknown	Het
Pyroxd2	T	A	19: 42,740,459	K167N	probably benign	Het
Rnf123	T	C	9: 108,070,731	Y131C	probably damaging	Het
Rnf25	T	C	1: 74,595,252	D204G	probably damaging	Het
Rpf2	G	A	10: 40,233,185	T60I	possibly damaging	Het
Scaf11	T	C	15: 96,419,458	N742D	probably benign	Het
Shank1	G	A	7: 44,352,651	D1257N	possibly damaging	Het
Shc2	A	G	10: 79,630,099	V168A	probably damaging	Het
Slc16a4	G	A	3: 107,292,442	V2M	probably benign	Het
Slc24a3	T	C	2: 145,613,574	V461A	probably benign	Het
Slc45a2	C	T	15: 11,027,785	T480I	probably damaging	Het
Slc4a4	T	A	5: 89,197,764		probably null	Het
Snx30	T	A	4: 59,879,329	D189E	probably benign	Het
Sspo	A	G	6: 48,495,260	Q4653R	possibly damaging	Het
Tpte	G	T	8: 22,327,790	R264I	probably damaging	Het
Tsc22d4	T	A	5: 137,758,774		probably benign	Het
Ubn1	T	C	16: 5,074,369	L585P	possibly damaging	Het
Usp24	A	T	4: 106,408,013	D1781V	probably damaging	Het
Utp20	A	T	10: 88,772,915	Y1514*	probably null	Het
Vmn1r55	A	T	7: 5,146,996	Y143N	probably damaging	Het
Vmn2r65	A	G	7: 84,946,654	V274A	probably benign	Het
Wdr17	A	G	8: 54,639,489	S1087P	possibly damaging	Het
Wnt5b	C	A	6: 119,440,322	R156L	probably damaging	Het
Zan	T	C	5: 137,435,634	D2279G	unknown	Het
Zan	T	C	5: 137,464,074	T948A	unknown	Het
Zfp758	T	C	17: 22,372,068	S4P	probably damaging	Het
Zfp839	A	G	12: 110,855,586	E278G	probably benign	Het
Zfp994	T	A	17: 22,200,525	H481L	probably damaging	Het
Zswim9	G	A	7: 13,260,983	R416C	probably damaging	Het

Other mutations in Myo18a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Myo18a	APN	11	77847938	missense	probably damaging	1.00
IGL00753:Myo18a	APN	11	77825151	missense	probably damaging	1.00
IGL01137:Myo18a	APN	11	77827829	missense	probably damaging	1.00
IGL01536:Myo18a	APN	11	77820851	missense	probably damaging	1.00
IGL01642:Myo18a	APN	11	77864732	missense	probably benign	0.07
IGL01728:Myo18a	APN	11	77777856	missense	probably damaging	0.99
IGL01780:Myo18a	APN	11	77850247	missense	probably benign	0.02
IGL02286:Myo18a	APN	11	77777985	nonsense	probably null
IGL02350:Myo18a	APN	11	77850247	missense	probably benign	0.02
IGL02357:Myo18a	APN	11	77850247	missense	probably benign	0.02
IGL02420:Myo18a	APN	11	77818693	missense	possibly damaging	0.81
IGL02643:Myo18a	APN	11	77778172	missense	possibly damaging	0.67
IGL02667:Myo18a	APN	11	77857852	splice site	probably benign
IGL02869:Myo18a	APN	11	77864786	missense	probably damaging	1.00
IGL02869:Myo18a	APN	11	77829873	splice site	probably benign
IGL02962:Myo18a	APN	11	77778235	missense	probably damaging	1.00
IGL02963:Myo18a	APN	11	77842018	splice site	probably benign
IGL03410:Myo18a	APN	11	77848004	missense	probably damaging	0.99
IGL03050:Myo18a	UTSW	11	77818770	missense	probably benign	0.00
R0022:Myo18a	UTSW	11	77843233	critical splice donor site	probably null
R0064:Myo18a	UTSW	11	77847344	missense	probably damaging	1.00
R0064:Myo18a	UTSW	11	77847344	missense	probably damaging	1.00
R0098:Myo18a	UTSW	11	77845765	missense	probably damaging	1.00
R0322:Myo18a	UTSW	11	77829800	missense	probably damaging	1.00
R0373:Myo18a	UTSW	11	77821042	missense	probably benign	0.01
R0379:Myo18a	UTSW	11	77850806	missense	possibly damaging	0.84
R0513:Myo18a	UTSW	11	77811594	intron	probably benign
R0688:Myo18a	UTSW	11	77824140	missense	probably damaging	1.00
R0734:Myo18a	UTSW	11	77847404	missense	probably damaging	1.00
R0790:Myo18a	UTSW	11	77840709	missense	possibly damaging	0.95
R1099:Myo18a	UTSW	11	77818901	splice site	probably null
R1103:Myo18a	UTSW	11	77823330	missense	probably damaging	1.00
R1183:Myo18a	UTSW	11	77857745	missense	probably damaging	1.00
R1216:Myo18a	UTSW	11	77818647	missense	probably benign	0.35
R1331:Myo18a	UTSW	11	77841579	missense	probably benign	0.28
R1479:Myo18a	UTSW	11	77842194	missense	probably benign	0.04
R1723:Myo18a	UTSW	11	77853314	missense	probably damaging	0.97
R1742:Myo18a	UTSW	11	77841467	missense	probably damaging	0.99
R1796:Myo18a	UTSW	11	77829344	missense	possibly damaging	0.94
R1823:Myo18a	UTSW	11	77825097	splice site	probably benign
R1827:Myo18a	UTSW	11	77818771	missense	probably benign	0.00
R2033:Myo18a	UTSW	11	77843099	splice site	probably null
R2043:Myo18a	UTSW	11	77823363	missense	probably damaging	0.99
R2105:Myo18a	UTSW	11	77850234	missense	probably benign
R2191:Myo18a	UTSW	11	77818615	missense	probably damaging	0.99
R2264:Myo18a	UTSW	11	77819972	splice site	probably benign
R2370:Myo18a	UTSW	11	77777770	missense	probably benign	0.03
R3015:Myo18a	UTSW	11	77859020	intron	probably benign
R3433:Myo18a	UTSW	11	77818044	splice site	probably null
R3739:Myo18a	UTSW	11	77845615	missense	probably damaging	1.00
R3825:Myo18a	UTSW	11	77777466	missense	possibly damaging	0.47
R4056:Myo18a	UTSW	11	77812013	missense	possibly damaging	0.72
R4163:Myo18a	UTSW	11	77829708	missense	possibly damaging	0.72
R4184:Myo18a	UTSW	11	77857787	missense	probably damaging	1.00
R4620:Myo18a	UTSW	11	77817947	missense	possibly damaging	0.93
R4628:Myo18a	UTSW	11	77824136	missense	probably damaging	1.00
R4647:Myo18a	UTSW	11	77817950	missense	probably damaging	1.00
R4701:Myo18a	UTSW	11	77817665	missense	probably damaging	1.00
R4729:Myo18a	UTSW	11	77777685	splice site	probably null
R4731:Myo18a	UTSW	11	77829759	missense	probably benign	0.00
R4739:Myo18a	UTSW	11	77823323	missense	probably damaging	1.00
R4814:Myo18a	UTSW	11	77859236	intron	probably benign
R4889:Myo18a	UTSW	11	77832412	missense	probably damaging	1.00
R4988:Myo18a	UTSW	11	77845521	critical splice donor site	probably null
R5172:Myo18a	UTSW	11	77824098	missense	probably damaging	1.00
R5177:Myo18a	UTSW	11	77864842	utr 3 prime	probably benign
R5643:Myo18a	UTSW	11	77854687	missense	probably benign	0.12
R5808:Myo18a	UTSW	11	77829301	missense	probably benign	0.34
R5871:Myo18a	UTSW	11	77832480	missense	probably damaging	1.00
R5936:Myo18a	UTSW	11	77818213	missense	probably damaging	1.00
R6017:Myo18a	UTSW	11	77841523	missense	probably damaging	0.96
R6053:Myo18a	UTSW	11	77818176	missense	probably damaging	1.00
R6271:Myo18a	UTSW	11	77820809	missense	probably damaging	1.00
R6486:Myo18a	UTSW	11	77864822	missense	possibly damaging	0.83
R6558:Myo18a	UTSW	11	77850852	missense	probably damaging	0.99
R6884:Myo18a	UTSW	11	77819049	missense	possibly damaging	0.67
R6983:Myo18a	UTSW	11	77845515	missense	probably benign	0.06
R6993:Myo18a	UTSW	11	77859074	intron	probably benign
R7071:Myo18a	UTSW	11	77823827	missense	probably damaging	1.00
R7074:Myo18a	UTSW	11	77842561	missense	probably benign	0.03
R7238:Myo18a	UTSW	11	77842233	missense	probably damaging	0.96
R7328:Myo18a	UTSW	11	77807911	missense
R7527:Myo18a	UTSW	11	77843580	missense	probably benign	0.00
R7598:Myo18a	UTSW	11	77847346	missense	probably damaging	1.00
R7671:Myo18a	UTSW	11	77859420	missense
R7958:Myo18a	UTSW	11	77841557	missense	probably damaging	1.00
R8098:Myo18a	UTSW	11	77845401	missense	probably damaging	0.97
R8168:Myo18a	UTSW	11	77821142	missense	probably damaging	0.99
R8318:Myo18a	UTSW	11	77823389	missense	probably benign	0.02
Y5407:Myo18a	UTSW	11	77777815	missense	probably benign	0.44
Z1177:Myo18a	UTSW	11	77841995	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTAGCATCCCTTAGCCATAGC -3'
(R):5'- CCCATGGTGAGGTAAGCATATAC -3'

Sequencing Primer
(F):5'- GCTTGGCCAGACTTCCTG -3'
(R):5'- TATACTTAACAGGACTTGGGGACTG -3'

Posted On

2016-08-04