Mutagenetix > Incidental Mutations

Incidental Mutation 'R4809:Fn1'

426226

Institutional Source

Beutler Lab

Gene Symbol

Fn1

Ensembl Gene

ENSMUSG00000026193

Gene Name

fibronectin 1

Synonyms

Fn-1, Fn

MMRRC Submission

042428-MU

Accession Numbers

Is this an essential gene?

Essential (E-score: 1.000) question?

Stock #

R4809 (G1)

Quality Score

130

Status

Validated

Chromosome

Chromosomal Location

71585520-71653200 bp(-) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

C to A at 71652800 bp

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055226] [ENSMUST00000186129] [ENSMUST00000186736] [ENSMUST00000186940] [ENSMUST00000187938] [ENSMUST00000188674] [ENSMUST00000188894] [ENSMUST00000189821] [ENSMUST00000190780]

Predicted Effect

probably benign
Transcript: ENSMUST00000055226

SMART Domains

Protein: ENSMUSP00000054499
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1264	1346	4.22e-9	SMART
FN3	1357	1437	9.6e-13	SMART
FN3	1448	1527	1.82e-13	SMART
FN3	1538	1617	6.69e-12	SMART
FN3	1632	1711	2.72e-12	SMART
FN3	1722	1801	8.9e-8	SMART
FN3	1812	1891	1.66e-7	SMART
FN3	1904	1983	4.92e-10	SMART
FN3	1993	2074	3.64e-13	SMART
low complexity region	2148	2165	N/A	INTRINSIC
FN3	2193	2272	2.9e0	SMART
FN1	2296	2340	3.72e-19	SMART
FN1	2341	2383	2.49e-20	SMART
FN1	2385	2425	2.69e-16	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000097699

SMART Domains

Protein: ENSMUSP00000095305
Gene: ENSMUSG00000073652

Domain	Start	End	E-Value	Type
low complexity region	40	67	N/A	INTRINSIC
low complexity region	96	108	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125934

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000126516

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127218

Predicted Effect

probably benign
Transcript: ENSMUST00000186129

SMART Domains

Protein: ENSMUSP00000141123
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1967	1984	N/A	INTRINSIC
FN3	2012	2091	2.9e0	SMART
FN1	2115	2159	3.72e-19	SMART
FN1	2160	2202	2.49e-20	SMART
FN1	2204	2244	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186736

SMART Domains

Protein: ENSMUSP00000140372
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
FN1	53	96	3.3e-21	SMART
FN1	98	142	1.7e-21	SMART
Pfam:fn1	143	160	1.2e-4	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186879

Predicted Effect

probably benign
Transcript: ENSMUST00000186940

SMART Domains

Protein: ENSMUSP00000140315
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	1.77e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187938

SMART Domains

Protein: ENSMUSP00000140975
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2032	2049	N/A	INTRINSIC
FN3	2077	2156	2.9e0	SMART
FN1	2180	2224	3.72e-19	SMART
FN1	2225	2267	2.49e-20	SMART
FN1	2269	2309	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188674

SMART Domains

Protein: ENSMUSP00000140907
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1981	6.79e-13	SMART
FN3	1983	2061	1.01e1	SMART
FN1	2085	2129	3.72e-19	SMART
FN1	2130	2172	2.49e-20	SMART
FN1	2174	2214	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188894

SMART Domains

Protein: ENSMUSP00000140471
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2057	2074	N/A	INTRINSIC
FN3	2102	2181	2.9e0	SMART
FN1	2205	2249	3.72e-19	SMART
FN1	2250	2292	2.49e-20	SMART
FN1	2294	2334	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000189821

SMART Domains

Protein: ENSMUSP00000139702
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1891	6.79e-13	SMART
FN3	1893	1971	1.01e1	SMART
FN1	1995	2039	3.72e-19	SMART
FN1	2040	2082	2.49e-20	SMART
FN1	2084	2124	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000190780

SMART Domains

Protein: ENSMUSP00000140816
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1942	1959	N/A	INTRINSIC
FN3	1987	2066	2.9e0	SMART
FN1	2090	2134	3.72e-19	SMART
FN1	2135	2177	2.49e-20	SMART
FN1	2179	2219	2.69e-16	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

96% (96/100)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mutants are defective in mesodermal function. Null mutants are embryonic lethal with major patterning and organizational defects. Conditional mutants live and show increased neuronal apoptosis and susceptibility to induced cerebral ischemia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 87 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3425401B19Rik	A	G	14: 32,662,631	V459A	probably benign	Het
Abca12	C	T	1: 71,278,856	A1840T	probably benign	Het
Abhd6	T	G	14: 8,039,771	M1R	probably null	Het
Abl1	T	C	2: 31,800,242	L572P	probably damaging	Het
Adamts2	C	A	11: 50,803,690	S1101R	probably benign	Het
Adgra2	G	A	8: 27,110,479	W200*	probably null	Het
AI661453	C	A	17: 47,467,187		probably benign	Het
Aldh1l2	A	G	10: 83,506,632	F438S	probably damaging	Het
Ankrd49	G	A	9: 14,781,214	T218I	possibly damaging	Het
Ano7	T	C	1: 93,394,566	F410L	probably benign	Het
Aox4	T	A	1: 58,266,649	F1271I	probably damaging	Het
Aqr	T	C	2: 114,175,214		probably benign	Het
Arhgap42	A	G	9: 9,180,117	S54P	probably damaging	Het
Aunip	C	A	4: 134,511,139	D16E	possibly damaging	Het
Btbd7	A	G	12: 102,793,744		probably null	Het
Cabp4	T	C	19: 4,139,291	H89R	probably benign	Het
Ccni	AAA	AAACTAA	5: 93,187,570		probably benign	Het
Chfr	C	T	5: 110,158,834	H410Y	probably damaging	Het
Churc1	C	A	12: 76,782,897	L111M	probably damaging	Het
Clasp1	C	T	1: 118,461,250	T113I	probably benign	Het
Col12a1	T	A	9: 79,693,567	Q745L	probably benign	Het
Col20a1	T	C	2: 180,998,661	L537P	probably damaging	Het
Creb5	A	T	6: 53,610,426	E47V	probably null	Het
Csnk1d	A	T	11: 120,963,842		probably benign	Het
Cts6	A	T	13: 61,202,181	W29R	probably damaging	Het
Dbt	T	A	3: 116,546,343	I420N	probably damaging	Het
Det1	C	A	7: 78,843,807	D150Y	probably damaging	Het
Dlk2	A	G	17: 46,299,014		probably null	Het
Dnmt3a	A	T	12: 3,900,352	I639F	probably damaging	Het
Dock9	A	T	14: 121,546,596	Y1989N	probably benign	Het
Dsg4	A	T	18: 20,466,621	T765S	possibly damaging	Het
Epha5	C	T	5: 84,105,891	D548N	possibly damaging	Het
Fam189a1	G	A	7: 64,776,740	T159I	probably damaging	Het
Fam227b	T	A	2: 126,116,125	Y240F	possibly damaging	Het
Fbxw21	C	T	9: 109,143,390	V395I	probably damaging	Het
Fpr-rs3	A	T	17: 20,624,421	S153T	probably benign	Het
Frem2	A	G	3: 53,653,895	F1064L	probably benign	Het
Gas2l3	CACTCGTCATACT	CACT	10: 89,430,958		probably benign	Het
Gjd2	C	T	2: 114,011,541	G152R	probably damaging	Het
Gpr75	T	C	11: 30,892,154	I353T	possibly damaging	Het
Grb7	T	C	11: 98,451,436	V145A	possibly damaging	Het
Igkv4-73	G	A	6: 69,197,823	R40W	unknown	Het
Kif1c	C	T	11: 70,726,357	A839V	probably benign	Het
Krt31	G	A	11: 100,049,922	A125V	possibly damaging	Het
Lamb1	A	G	12: 31,278,526	Y163C	probably damaging	Het
Mars	G	T	10: 127,300,215	T535K	probably damaging	Het
Mdc1	T	C	17: 35,849,101		probably null	Het
Micu1	C	T	10: 59,740,822	H167Y	probably benign	Het
Minos1	C	G	4: 139,130,957	W10S	probably damaging	Het
Mrgprb1	C	T	7: 48,447,991	V58I	possibly damaging	Het
Ncoa7	T	A	10: 30,771,762	E6V	possibly damaging	Het
Nectin3	A	C	16: 46,448,160		probably benign	Het
Olfr1085	T	A	2: 86,657,685	M258L	possibly damaging	Het
Olfr1294	C	T	2: 111,537,611	C226Y	probably benign	Het
Olfr46	A	G	7: 140,611,074	K295E	probably damaging	Het
Olfr598	T	A	7: 103,328,523	Y12*	probably null	Het
Pex16	T	C	2: 92,376,638	S54P	probably damaging	Het
Pik3cg	A	T	12: 32,204,081	S636T	possibly damaging	Het
Plin5	A	G	17: 56,116,855	S27P	probably benign	Het
Ptch1	A	T	13: 63,513,708	D1068E	probably damaging	Het
Ptprq	T	C	10: 107,563,175	T1960A	probably damaging	Het
Rap1gap2	T	C	11: 74,407,974		probably benign	Het
Rcc2	G	A	4: 140,717,042	R348Q	probably damaging	Het
Rhbdd3	C	A	11: 5,105,949	A377D	probably damaging	Het
Rpl7	T	G	1: 16,101,965		probably benign	Het
Scn11a	G	T	9: 119,819,870	D42E	probably benign	Het
Scube3	C	T	17: 28,165,173	R549W	probably damaging	Het
Sil1	A	T	18: 35,325,375	M189K	probably damaging	Het
Slc39a6	G	A	18: 24,585,474	Q225*	probably null	Het
Slc7a15	A	T	12: 8,539,002	C182S	probably benign	Het
Spata21	G	A	4: 141,097,120		probably null	Het
Stim2	T	C	5: 54,110,613	V417A	probably damaging	Het
Szt2	T	C	4: 118,388,985	D993G	probably damaging	Het
Tet3	A	G	6: 83,402,946	S747P	probably benign	Het
Tmem86a	C	G	7: 47,052,930	S34R	possibly damaging	Het
Top2b	T	A	14: 16,383,125	S38T	probably benign	Het
Trim33	A	T	3: 103,329,256	T561S	possibly damaging	Het
Ttc39a	C	T	4: 109,416,021	Q25*	probably null	Het
Urb1	A	G	16: 90,759,842	I1816T	possibly damaging	Het
Usp24	T	C	4: 106,413,676		probably null	Het
Usp36	A	C	11: 118,263,070	L840R	probably damaging	Het
Usp50	C	A	2: 126,777,853		probably benign	Het
Vangl2	A	G	1: 172,009,663	V193A	possibly damaging	Het
Vmn1r62	T	A	7: 5,675,867	H182Q	probably benign	Het
Vmn2r124	A	T	17: 18,073,745	Y698F	probably benign	Het
Wls	C	T	3: 159,897,445	T165I	probably benign	Het
Zfp808	G	T	13: 62,171,292	E112*	probably null	Het

Other mutations in Fn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Fn1	APN	1	71652873	missense	probably benign	0.28
IGL00402:Fn1	APN	1	71641163	missense	probably damaging	1.00
IGL00946:Fn1	APN	1	71645540	splice site	probably benign
IGL01311:Fn1	APN	1	71628140	missense	probably damaging	1.00
IGL01338:Fn1	APN	1	71626210	missense	probably damaging	0.98
IGL01353:Fn1	APN	1	71586939	missense	probably damaging	1.00
IGL01674:Fn1	APN	1	71606741	missense	probably damaging	1.00
IGL01701:Fn1	APN	1	71629853	splice site	probably benign
IGL01734:Fn1	APN	1	71619485	missense	probably damaging	1.00
IGL01788:Fn1	APN	1	71613837	missense	probably damaging	1.00
IGL02186:Fn1	APN	1	71638534	missense	probably damaging	1.00
IGL02398:Fn1	APN	1	71618670	splice site	probably null
IGL02425:Fn1	APN	1	71641143	splice site	probably benign
IGL02516:Fn1	APN	1	71637323	missense	possibly damaging	0.78
IGL02593:Fn1	APN	1	71602432	missense	probably benign
IGL02651:Fn1	APN	1	71597676	missense	possibly damaging	0.65
IGL02681:Fn1	APN	1	71619482	missense	probably damaging	1.00
IGL02890:Fn1	APN	1	71598372	critical splice donor site	probably null
IGL02929:Fn1	APN	1	71595662	critical splice donor site	probably null
IGL03036:Fn1	APN	1	71629773	missense	probably damaging	1.00
IGL03088:Fn1	APN	1	71614038	splice site	probably null
IGL03142:Fn1	APN	1	71637296	missense	probably damaging	1.00
IGL03172:Fn1	APN	1	71641262	missense	probably damaging	0.99
IGL03184:Fn1	APN	1	71609497	missense	probably benign	0.02
IGL03212:Fn1	APN	1	71641325	nonsense	probably null
IGL03246:Fn1	APN	1	71624296	missense	possibly damaging	0.89
IGL03367:Fn1	APN	1	71597553	missense	probably benign	0.27
R3906_Fn1_168	UTSW	1	71607913	missense	probably damaging	1.00
PIT4514001:Fn1	UTSW	1	71628456	missense	probably benign	0.01
R0008:Fn1	UTSW	1	71595720	missense	probably damaging	0.98
R0112:Fn1	UTSW	1	71609653	missense	probably damaging	1.00
R0138:Fn1	UTSW	1	71624110	missense	possibly damaging	0.82
R0383:Fn1	UTSW	1	71597685	missense	probably damaging	0.99
R0386:Fn1	UTSW	1	71595786	missense	probably damaging	1.00
R0648:Fn1	UTSW	1	71597585	missense	possibly damaging	0.79
R0684:Fn1	UTSW	1	71595809	splice site	probably null
R1054:Fn1	UTSW	1	71586214	makesense	probably null
R1183:Fn1	UTSW	1	71586245	missense	probably damaging	0.98
R1405:Fn1	UTSW	1	71642078	missense	probably damaging	1.00
R1405:Fn1	UTSW	1	71642078	missense	probably damaging	1.00
R1414:Fn1	UTSW	1	71601303	splice site	probably benign
R1677:Fn1	UTSW	1	71597655	missense	probably benign	0.00
R1773:Fn1	UTSW	1	71637383	missense	probably damaging	1.00
R1830:Fn1	UTSW	1	71624259	missense	probably damaging	1.00
R1987:Fn1	UTSW	1	71651625	missense	probably damaging	1.00
R1989:Fn1	UTSW	1	71651625	missense	probably damaging	1.00
R2068:Fn1	UTSW	1	71600439	missense	probably damaging	1.00
R2113:Fn1	UTSW	1	71626164	missense	probably damaging	1.00
R2145:Fn1	UTSW	1	71606004	missense	probably damaging	1.00
R2246:Fn1	UTSW	1	71628535	missense	probably benign	0.10
R2273:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2274:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2275:Fn1	UTSW	1	71613943	missense	probably null	1.00
R2303:Fn1	UTSW	1	71614036	critical splice acceptor site	probably null
R2379:Fn1	UTSW	1	71649284	nonsense	probably null
R2382:Fn1	UTSW	1	71648119	missense	probably damaging	1.00
R2567:Fn1	UTSW	1	71597736	nonsense	probably null
R2864:Fn1	UTSW	1	71602419	missense	probably damaging	0.99
R3154:Fn1	UTSW	1	71593083	missense	probably damaging	1.00
R3837:Fn1	UTSW	1	71653155	splice site	probably null
R3844:Fn1	UTSW	1	71609574	missense	possibly damaging	0.61
R3886:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3887:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3888:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3889:Fn1	UTSW	1	71640306	missense	probably damaging	1.00
R3905:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3906:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3907:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R3909:Fn1	UTSW	1	71607913	missense	probably damaging	1.00
R4611:Fn1	UTSW	1	71624178	nonsense	probably null
R4724:Fn1	UTSW	1	71648148	critical splice acceptor site	probably null
R4732:Fn1	UTSW	1	71602512	splice site	probably null
R4733:Fn1	UTSW	1	71602512	splice site	probably null
R4756:Fn1	UTSW	1	71590808	missense	probably damaging	1.00
R4839:Fn1	UTSW	1	71642083	missense	probably damaging	1.00
R4915:Fn1	UTSW	1	71595809	splice site	probably null
R4917:Fn1	UTSW	1	71595809	splice site	probably null
R4918:Fn1	UTSW	1	71595809	splice site	probably null
R5002:Fn1	UTSW	1	71629728	missense	possibly damaging	0.48
R5015:Fn1	UTSW	1	71626177	missense	probably damaging	0.98
R5022:Fn1	UTSW	1	71624179	missense	probably damaging	1.00
R5109:Fn1	UTSW	1	71649235	missense	probably damaging	1.00
R5267:Fn1	UTSW	1	71629704	missense	probably damaging	1.00
R5323:Fn1	UTSW	1	71597432	missense	probably benign	0.09
R5333:Fn1	UTSW	1	71624180	missense	probably damaging	1.00
R5631:Fn1	UTSW	1	71590196	missense	probably damaging	1.00
R5644:Fn1	UTSW	1	71627250	missense	probably damaging	1.00
R5754:Fn1	UTSW	1	71600322	missense	probably damaging	1.00
R5807:Fn1	UTSW	1	71648059	missense	probably damaging	1.00
R6053:Fn1	UTSW	1	71599290	missense	probably damaging	1.00
R6133:Fn1	UTSW	1	71597727	missense	probably damaging	1.00
R6186:Fn1	UTSW	1	71637290	missense	probably damaging	1.00
R6270:Fn1	UTSW	1	71637275	missense	probably damaging	1.00
R6332:Fn1	UTSW	1	71628071	missense	probably benign	0.01
R6431:Fn1	UTSW	1	71647844	splice site	probably null
R6571:Fn1	UTSW	1	71626190	missense	probably damaging	1.00
R6596:Fn1	UTSW	1	71609482	missense	probably damaging	1.00
R6862:Fn1	UTSW	1	71613907	missense	probably benign	0.43
R6898:Fn1	UTSW	1	71600413	missense	probably damaging	1.00
R6984:Fn1	UTSW	1	71626079	missense	probably damaging	1.00
R7107:Fn1	UTSW	1	71627249	missense	probably damaging	1.00
R7121:Fn1	UTSW	1	71600538	intron	probably benign
R7127:Fn1	UTSW	1	71597544	missense	probably benign	0.16
R7194:Fn1	UTSW	1	71602323	missense	probably damaging	1.00
R7274:Fn1	UTSW	1	71628113	missense	probably benign
R7285:Fn1	UTSW	1	71637339	missense	probably damaging	1.00
R7426:Fn1	UTSW	1	71649225	missense	probably damaging	1.00
R7453:Fn1	UTSW	1	71590880	missense	probably damaging	1.00
R7508:Fn1	UTSW	1	71597516	missense	probably benign	0.01
R7724:Fn1	UTSW	1	71603735	missense	probably benign	0.02
R7848:Fn1	UTSW	1	71650601	missense	probably damaging	1.00
R7992:Fn1	UTSW	1	71599666	missense	probably benign	0.34
R8036:Fn1	UTSW	1	71590151	nonsense	probably null
R8077:Fn1	UTSW	1	71612602	missense	probably damaging	1.00
R8175:Fn1	UTSW	1	71599665	missense	probably damaging	1.00
R8177:Fn1	UTSW	1	71609587	missense	probably benign
R8212:Fn1	UTSW	1	71642905	missense	probably benign	0.01
R8322:Fn1	UTSW	1	71628459	missense	probably benign	0.04
R8745:Fn1	UTSW	1	71637369	missense	probably benign	0.00
X0023:Fn1	UTSW	1	71598373	critical splice donor site	probably null
Z1088:Fn1	UTSW	1	71649292	missense	probably damaging	1.00
Z1176:Fn1	UTSW	1	71597411	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- AGAATTTGTTAGCGCACTTGAG -3'
(R):5'- ACTCAAGATGCTCAGGGGTC -3'

Sequencing Primer
(F):5'- GGAAGAACAGACGGTCCCTAC -3'
(R):5'- GCTCAGGGGTCCGGGAC -3'

Posted On

2016-08-25