Incidental Mutation 'R5427:Zfp963'
ID426961
Institutional Source Beutler Lab
Gene Symbol Zfp963
Ensembl Gene ENSMUSG00000092260
Gene Namezinc finger protein 963
Synonyms
MMRRC Submission 042993-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.065) question?
Stock #R5427 (G1)
Quality Score225
Status Validated
Chromosome8
Chromosomal Location69741639-69749970 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 69743456 bp
ZygosityHeterozygous
Amino Acid Change Serine to Proline at position 116 (S116P)
Ref Sequence ENSEMBL: ENSMUSP00000119087 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000130458] [ENSMUST00000149782] [ENSMUST00000154063]
Predicted Effect unknown
Transcript: ENSMUST00000126915
AA Change: S127P
SMART Domains Protein: ENSMUSP00000122850
Gene: ENSMUSG00000092544
AA Change: S127P

DomainStartEndE-ValueType
KRAB 16 73 1.96e-17 SMART
PDB:2I13|B 104 210 3e-6 PDB
SCOP:d1fgja_ 114 216 2e-6 SMART
Blast:KRAB 189 209 3e-6 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000130458
AA Change: S116P

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000119087
Gene: ENSMUSG00000092260
AA Change: S116P

DomainStartEndE-ValueType
KRAB 4 61 1.96e-17 SMART
ZnF_C2H2 162 184 7.37e1 SMART
ZnF_C2H2 213 235 1.69e-3 SMART
ZnF_C2H2 240 262 8.47e-4 SMART
ZnF_C2H2 268 290 7.15e-2 SMART
ZnF_C2H2 296 318 3.83e-2 SMART
ZnF_C2H2 324 346 4.17e-3 SMART
ZnF_C2H2 352 374 5.99e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000149782
SMART Domains Protein: ENSMUSP00000123252
Gene: ENSMUSG00000092544

DomainStartEndE-ValueType
KRAB 80 139 1.2e-17 SMART
ZnF_C2H2 155 177 1.69e-3 SMART
ZnF_C2H2 182 204 8.47e-4 SMART
ZnF_C2H2 210 232 7.15e-2 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000154063
SMART Domains Protein: ENSMUSP00000117867
Gene: ENSMUSG00000092260

DomainStartEndE-ValueType
KRAB 4 61 1.96e-17 SMART
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.3%
Validation Efficiency 97% (58/60)
Allele List at MGI
Other mutations in this stock
Total: 56 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700057G04Rik G A 9: 92,352,596 C128Y probably benign Het
Aaas A G 15: 102,339,950 V277A possibly damaging Het
Adcy7 G A 8: 88,326,201 probably null Het
Adgrg5 A T 8: 94,935,102 D157V probably benign Het
Akap13 A G 7: 75,728,869 N2090S possibly damaging Het
Alpi T C 1: 87,101,354 N33D probably benign Het
Anapc15 C T 7: 101,898,603 P68L probably damaging Het
Ankrd34a G A 3: 96,597,521 G14R probably damaging Het
Anp32a A G 9: 62,377,316 probably benign Het
Atg4b G C 1: 93,775,206 K86N probably damaging Het
Bbx T C 16: 50,280,497 T12A probably benign Het
Catsperg2 T C 7: 29,714,850 T377A possibly damaging Het
Ccdc158 T C 5: 92,648,962 Q505R probably damaging Het
Cep135 T A 5: 76,638,202 S1051T probably benign Het
Cryab A G 9: 50,756,293 D109G probably damaging Het
Crym A C 7: 120,199,222 probably benign Het
Csf2rb2 T A 15: 78,288,911 S250C probably damaging Het
Diaph1 A T 18: 37,890,595 V730E unknown Het
Eci3 G T 13: 34,959,948 L65M possibly damaging Het
Erg28 T C 12: 85,819,567 N46D probably damaging Het
Fam89b A G 19: 5,728,791 S127P probably benign Het
Fign A G 2: 63,978,998 Y643H probably damaging Het
Galk2 T C 2: 125,946,821 V265A probably benign Het
Gclm T C 3: 122,266,327 V252A probably damaging Het
Git2 A G 5: 114,730,328 S584P possibly damaging Het
Gm38394 A G 1: 133,657,595 V668A possibly damaging Het
Iqcf3 T C 9: 106,543,860 probably null Het
Kcnk3 A G 5: 30,622,295 T230A possibly damaging Het
Myh10 T C 11: 68,802,931 L1486P probably damaging Het
Myom2 C A 8: 15,113,764 A1006E probably benign Het
Myt1l G A 12: 29,832,332 G509R unknown Het
Nampt A T 12: 32,834,915 H111L probably benign Het
Nid1 A G 13: 13,483,683 Y671C probably damaging Het
Npy5r G A 8: 66,681,020 R374C probably damaging Het
Olfr1116 A T 2: 87,269,514 K244N probably benign Het
Olfr1487 A T 19: 13,619,350 S20C probably benign Het
Palld C T 8: 61,550,072 C720Y probably benign Het
Pcsk6 C T 7: 66,033,899 T606M probably benign Het
Pfas T C 11: 69,001,153 I176M possibly damaging Het
Pi4kb A G 3: 94,994,207 D395G probably benign Het
Plod3 A T 5: 136,991,788 Y547F probably damaging Het
Pnpo T C 11: 96,943,807 Y21C probably benign Het
Rgs1 A T 1: 144,246,280 C118* probably null Het
Rrnad1 T C 3: 87,924,332 probably benign Het
Slc22a27 A G 19: 7,879,388 probably null Het
Sntb1 C G 15: 55,642,795 G461R probably damaging Het
Sppl2c G A 11: 104,187,867 V498I probably benign Het
Stim2 A T 5: 54,110,939 I448F possibly damaging Het
Sulf1 A T 1: 12,796,912 T107S possibly damaging Het
Tenm3 A G 8: 48,236,564 V1996A probably damaging Het
Timm23 G A 14: 32,189,146 T171I possibly damaging Het
Tssk2 A G 16: 17,898,865 D44G probably damaging Het
Vmn2r28 A T 7: 5,486,377 Y488N probably damaging Het
Zbtb48 A G 4: 152,020,651 F518S probably damaging Het
Zfp709 A G 8: 71,889,132 E135G probably benign Het
Zfp788 G A 7: 41,649,652 V571I possibly damaging Het
Other mutations in Zfp963
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0220:Zfp963 UTSW 8 69743493 nonsense probably null
R0220:Zfp963 UTSW 8 69743495 missense probably benign 0.09
R0423:Zfp963 UTSW 8 69744506 missense probably damaging 1.00
R1171:Zfp963 UTSW 8 69743019 missense possibly damaging 0.86
R1630:Zfp963 UTSW 8 69744187 splice site probably benign
R1750:Zfp963 UTSW 8 69743450 missense possibly damaging 0.81
R2090:Zfp963 UTSW 8 69743346 nonsense probably null
R2185:Zfp963 UTSW 8 69742911 missense probably benign 0.43
R4484:Zfp963 UTSW 8 69744485 missense probably benign 0.29
R4600:Zfp963 UTSW 8 69742860 unclassified probably null
R4617:Zfp963 UTSW 8 69743294 missense probably benign 0.00
R5892:Zfp963 UTSW 8 69743377 missense probably benign
R5917:Zfp963 UTSW 8 69742860 unclassified probably null
R6073:Zfp963 UTSW 8 69743203 nonsense probably null
R7312:Zfp963 UTSW 8 69743109 missense probably damaging 1.00
R7739:Zfp963 UTSW 8 69742883 missense possibly damaging 0.59
Predicted Primers PCR Primer
(F):5'- TGTGAGCCTCTTGATGTGTTCCTA -3'
(R):5'- CAGGGTTTGCAGAGGGTTTC -3'

Sequencing Primer
(F):5'- TTCCTAGTGGATTTGGAAAACAGG -3'
(R):5'- GGAAACGCTCCTAGGACTTTGTATC -3'
Posted On2016-09-01