Mutagenetix > Incidental Mutations

Incidental Mutation 'R5441:Olfr1025-ps1'

427161

Institutional Source

Beutler Lab

Gene Symbol

Olfr1025-ps1

Ensembl Gene

ENSMUSG00000058884

Gene Name

olfactory receptor 1025, pseudogene 1

Synonyms

MOR196-6_p, GA_x6K02T2Q125-47397266-47398205, MOR196-5P

MMRRC Submission

043006-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R5441 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85915193-85921381 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85918590 bp

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Isoleucine at position 222 (F222I)

Ref Sequence

ENSEMBL: ENSMUSP00000149785 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056718] [ENSMUST00000188749] [ENSMUST00000213474] [ENSMUST00000217615] [ENSMUST00000219615]

Predicted Effect

probably benign
Transcript: ENSMUST00000056718

SMART Domains

Protein: ENSMUSP00000049887
Gene: ENSMUSG00000042863

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	6.1e-58	PFAM
Pfam:7tm_1	41	290	9e-25	PFAM

Predicted Effect

SMART Domains

Protein: ENSMUSP00000076311
Gene: ENSMUSG00000058884
AA Change: F222I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.5e-57	PFAM
Pfam:7tm_1	41	290	2.6e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188749
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000139860
Gene: ENSMUSG00000058884
AA Change: F222I

Domain	Start	End	E-Value	Type
low complexity region	26	40	N/A	INTRINSIC
Pfam:7tm_1	41	290	5.7e-30	PFAM
Pfam:7tm_4	139	283	7.8e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213474
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000217615

Predicted Effect

probably benign
Transcript: ENSMUST00000219615

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

98% (56/57)

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9530053A07Rik	C	T	7: 28,156,914	T2042M	probably damaging	Het
Abca12	A	G	1: 71,295,056	Y1096H	probably damaging	Het
Aspa	A	T	11: 73,305,594	F261I	probably damaging	Het
Atm	C	A	9: 53,516,467	G448*	probably null	Het
Atmin	T	A	8: 116,957,957	D785E	probably damaging	Het
C87414	A	T	5: 93,636,597	M139K	possibly damaging	Het
Car1	G	C	3: 14,776,304	R90G	probably damaging	Het
Cdh11	T	C	8: 102,647,546	D520G	probably benign	Het
Cers5	T	A	15: 99,751,238	K50*	probably null	Het
Chad	A	T	11: 94,568,292	D340V	probably benign	Het
Cspg5	T	C	9: 110,246,643	I68T	probably benign	Het
Fcrla	T	A	1: 170,925,422		probably benign	Het
Fer1l4	T	A	2: 156,023,257	D1608V	probably benign	Het
Fzd5	T	A	1: 64,735,417	Q395L	probably benign	Het
Gm7664	T	A	13: 62,528,650		probably benign	Het
Hk3	T	C	13: 55,015,056	E2G	probably damaging	Het
Hmcn2	G	A	2: 31,406,416	E2677K	possibly damaging	Het
Hydin	T	C	8: 110,565,109	L3411P	possibly damaging	Het
Ltbr	G	A	6: 125,312,794	R146W	probably damaging	Het
Lysmd2	C	T	9: 75,625,972	H70Y	possibly damaging	Het
Msi2	A	G	11: 88,479,992		probably benign	Het
Msi2	G	A	11: 88,718,095		probably benign	Het
Napsa	T	A	7: 44,581,393		probably benign	Het
Nlrp4a	T	A	7: 26,454,153	L710M	probably damaging	Het
Olfr103	T	C	17: 37,336,268		probably null	Het
Olfr1510	T	A	14: 52,409,957	K305M	probably benign	Het
Olfr459	C	T	6: 41,771,848	M150I	probably benign	Het
Olfr522	T	C	7: 140,162,651	T100A	probably benign	Het
Plbd2	T	A	5: 120,499,082	Y105F	probably benign	Het
Plppr5	A	T	3: 117,662,471	I214F	possibly damaging	Het
Ptcd3	A	G	6: 71,881,521	V655A	possibly damaging	Het
Ptpn14	T	C	1: 189,798,570	L78P	probably damaging	Het
Ralgapa1	A	C	12: 55,719,623	D1295E	probably damaging	Het
Rnf213	G	A	11: 119,409,020	D192N	probably damaging	Het
Ropn1	A	G	16: 34,666,797	I34M	probably damaging	Het
Rpe65	G	T	3: 159,604,401	G104C	probably damaging	Het
Scgb2b27	T	C	7: 34,013,157		probably benign	Het
Sh3bgr	T	C	16: 96,205,917	I29T	possibly damaging	Het
Smg1	T	C	7: 118,195,081		probably benign	Het
Stim2	T	A	5: 54,075,370	C68*	probably null	Het
Syne2	G	A	12: 75,989,143	V3736I	possibly damaging	Het
Tbx19	T	C	1: 165,153,680	N82D	probably damaging	Het
Tdp1	T	A	12: 99,910,285	V353D	probably damaging	Het
Tg	T	A	15: 66,696,520	I1352K	possibly damaging	Het
Thsd4	T	C	9: 59,979,783	T919A	probably damaging	Het
Tmprss11f	A	T	5: 86,528,203	M373K	probably damaging	Het
Tmprss15	T	A	16: 79,071,447		probably null	Het
Tpd52	A	T	3: 9,003,406	Y16*	probably null	Het
Ube2o	G	A	11: 116,544,442	R507C	probably damaging	Het
Unc93b1	T	C	19: 3,943,703	F382L	probably benign	Het
Vav2	A	T	2: 27,270,110		probably benign	Het
Vmn1r207-ps	T	C	13: 22,726,516		noncoding transcript	Het
Zan	T	A	5: 137,436,751	I2127F	unknown	Het
Zfp748	C	A	13: 67,540,618	C841F	probably damaging	Het
Zic4	C	A	9: 91,384,200	P299Q	probably damaging	Het

Other mutations in Olfr1025-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01660:Olfr1025-ps1	APN	2	85918564	missense	probably benign	0.25
IGL02881:Olfr1025-ps1	APN	2	85918116	missense	probably benign	0.00
R0097:Olfr1025-ps1	UTSW	2	85918840	missense	probably benign	0.00
R0097:Olfr1025-ps1	UTSW	2	85918840	missense	probably benign	0.00
R0324:Olfr1025-ps1	UTSW	2	85917951	missense	probably benign	0.22
R0392:Olfr1025-ps1	UTSW	2	85918762	missense	possibly damaging	0.81
R0765:Olfr1025-ps1	UTSW	2	85918705	missense	probably damaging	0.99
R1199:Olfr1025-ps1	UTSW	2	85918035	missense	probably benign	0.00
R1894:Olfr1025-ps1	UTSW	2	85918255	missense	probably benign	0.28
R2027:Olfr1025-ps1	UTSW	2	85918770	missense	probably damaging	0.99
R2141:Olfr1025-ps1	UTSW	2	85918827	missense	probably null
R3871:Olfr1025-ps1	UTSW	2	85918582	splice site	probably null
R4837:Olfr1025-ps1	UTSW	2	85918404	missense	probably benign	0.00
R4945:Olfr1025-ps1	UTSW	2	85918573	missense	possibly damaging	0.81
R5056:Olfr1025-ps1	UTSW	2	85918136	missense	probably damaging	1.00
R5960:Olfr1025-ps1	UTSW	2	85918725	missense	probably benign	0.07
R6807:Olfr1025-ps1	UTSW	2	85918038	missense	possibly damaging	0.48
R7320:Olfr1025-ps1	UTSW	2	85918374	missense	probably benign	0.00
R8523:Olfr1025-ps1	UTSW	2	85918063	missense	probably damaging	0.97
V1662:Olfr1025-ps1	UTSW	2	85918594	missense	probably benign	0.10
Z1176:Olfr1025-ps1	UTSW	2	85918501	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ATTTGCAAATGGCCTGGCAC -3'
(R):5'- GGTTCAACATCGGGCTTACAAAG -3'

Sequencing Primer
(F):5'- TGGCACAGACCATCCTGATG -3'
(R):5'- TCGGGCTTACAAAGATATAAAAGACC -3'

Posted On

2016-09-01