Incidental Mutation 'R5441:Olfr1025-ps1'
ID427161
Institutional Source Beutler Lab
Gene Symbol Olfr1025-ps1
Ensembl Gene ENSMUSG00000058884
Gene Nameolfactory receptor 1025, pseudogene 1
SynonymsMOR196-6_p, GA_x6K02T2Q125-47397266-47398205, MOR196-5P
MMRRC Submission 043006-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.084) question?
Stock #R5441 (G1)
Quality Score225
Status Validated
Chromosome2
Chromosomal Location85915193-85921381 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 85918590 bp
ZygosityHeterozygous
Amino Acid Change Phenylalanine to Isoleucine at position 222 (F222I)
Ref Sequence ENSEMBL: ENSMUSP00000149785 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000056718] [ENSMUST00000188749] [ENSMUST00000213474] [ENSMUST00000217615] [ENSMUST00000219615]
Predicted Effect probably benign
Transcript: ENSMUST00000056718
SMART Domains Protein: ENSMUSP00000049887
Gene: ENSMUSG00000042863

DomainStartEndE-ValueType
Pfam:7tm_4 31 307 6.1e-58 PFAM
Pfam:7tm_1 41 290 9e-25 PFAM
Predicted Effect
SMART Domains Protein: ENSMUSP00000076311
Gene: ENSMUSG00000058884
AA Change: F222I

DomainStartEndE-ValueType
Pfam:7tm_4 31 308 1.5e-57 PFAM
Pfam:7tm_1 41 290 2.6e-24 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000188749
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000139860
Gene: ENSMUSG00000058884
AA Change: F222I

DomainStartEndE-ValueType
low complexity region 26 40 N/A INTRINSIC
Pfam:7tm_1 41 290 5.7e-30 PFAM
Pfam:7tm_4 139 283 7.8e-48 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000213474
AA Change: F222I

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)
Predicted Effect probably benign
Transcript: ENSMUST00000217615
Predicted Effect probably benign
Transcript: ENSMUST00000219615
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.1%
  • 20x: 95.1%
Validation Efficiency 98% (56/57)
Allele List at MGI
Other mutations in this stock
Total: 55 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
9530053A07Rik C T 7: 28,156,914 T2042M probably damaging Het
Abca12 A G 1: 71,295,056 Y1096H probably damaging Het
Aspa A T 11: 73,305,594 F261I probably damaging Het
Atm C A 9: 53,516,467 G448* probably null Het
Atmin T A 8: 116,957,957 D785E probably damaging Het
C87414 A T 5: 93,636,597 M139K possibly damaging Het
Car1 G C 3: 14,776,304 R90G probably damaging Het
Cdh11 T C 8: 102,647,546 D520G probably benign Het
Cers5 T A 15: 99,751,238 K50* probably null Het
Chad A T 11: 94,568,292 D340V probably benign Het
Cspg5 T C 9: 110,246,643 I68T probably benign Het
Fcrla T A 1: 170,925,422 probably benign Het
Fer1l4 T A 2: 156,023,257 D1608V probably benign Het
Fzd5 T A 1: 64,735,417 Q395L probably benign Het
Gm7664 T A 13: 62,528,650 probably benign Het
Hk3 T C 13: 55,015,056 E2G probably damaging Het
Hmcn2 G A 2: 31,406,416 E2677K possibly damaging Het
Hydin T C 8: 110,565,109 L3411P possibly damaging Het
Ltbr G A 6: 125,312,794 R146W probably damaging Het
Lysmd2 C T 9: 75,625,972 H70Y possibly damaging Het
Msi2 A G 11: 88,479,992 probably benign Het
Msi2 G A 11: 88,718,095 probably benign Het
Napsa T A 7: 44,581,393 probably benign Het
Nlrp4a T A 7: 26,454,153 L710M probably damaging Het
Olfr103 T C 17: 37,336,268 probably null Het
Olfr1510 T A 14: 52,409,957 K305M probably benign Het
Olfr459 C T 6: 41,771,848 M150I probably benign Het
Olfr522 T C 7: 140,162,651 T100A probably benign Het
Plbd2 T A 5: 120,499,082 Y105F probably benign Het
Plppr5 A T 3: 117,662,471 I214F possibly damaging Het
Ptcd3 A G 6: 71,881,521 V655A possibly damaging Het
Ptpn14 T C 1: 189,798,570 L78P probably damaging Het
Ralgapa1 A C 12: 55,719,623 D1295E probably damaging Het
Rnf213 G A 11: 119,409,020 D192N probably damaging Het
Ropn1 A G 16: 34,666,797 I34M probably damaging Het
Rpe65 G T 3: 159,604,401 G104C probably damaging Het
Scgb2b27 T C 7: 34,013,157 probably benign Het
Sh3bgr T C 16: 96,205,917 I29T possibly damaging Het
Smg1 T C 7: 118,195,081 probably benign Het
Stim2 T A 5: 54,075,370 C68* probably null Het
Syne2 G A 12: 75,989,143 V3736I possibly damaging Het
Tbx19 T C 1: 165,153,680 N82D probably damaging Het
Tdp1 T A 12: 99,910,285 V353D probably damaging Het
Tg T A 15: 66,696,520 I1352K possibly damaging Het
Thsd4 T C 9: 59,979,783 T919A probably damaging Het
Tmprss11f A T 5: 86,528,203 M373K probably damaging Het
Tmprss15 T A 16: 79,071,447 probably null Het
Tpd52 A T 3: 9,003,406 Y16* probably null Het
Ube2o G A 11: 116,544,442 R507C probably damaging Het
Unc93b1 T C 19: 3,943,703 F382L probably benign Het
Vav2 A T 2: 27,270,110 probably benign Het
Vmn1r207-ps T C 13: 22,726,516 noncoding transcript Het
Zan T A 5: 137,436,751 I2127F unknown Het
Zfp748 C A 13: 67,540,618 C841F probably damaging Het
Zic4 C A 9: 91,384,200 P299Q probably damaging Het
Other mutations in Olfr1025-ps1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01660:Olfr1025-ps1 APN 2 85918564 missense probably benign 0.25
IGL02881:Olfr1025-ps1 APN 2 85918116 missense probably benign 0.00
R0097:Olfr1025-ps1 UTSW 2 85918840 missense probably benign 0.00
R0097:Olfr1025-ps1 UTSW 2 85918840 missense probably benign 0.00
R0324:Olfr1025-ps1 UTSW 2 85917951 missense probably benign 0.22
R0392:Olfr1025-ps1 UTSW 2 85918762 missense possibly damaging 0.81
R0765:Olfr1025-ps1 UTSW 2 85918705 missense probably damaging 0.99
R1199:Olfr1025-ps1 UTSW 2 85918035 missense probably benign 0.00
R1894:Olfr1025-ps1 UTSW 2 85918255 missense probably benign 0.28
R2027:Olfr1025-ps1 UTSW 2 85918770 missense probably damaging 0.99
R2141:Olfr1025-ps1 UTSW 2 85918827 missense probably null
R3871:Olfr1025-ps1 UTSW 2 85918582 unclassified probably null
R4837:Olfr1025-ps1 UTSW 2 85918404 missense probably benign 0.00
R4945:Olfr1025-ps1 UTSW 2 85918573 missense possibly damaging 0.81
R5056:Olfr1025-ps1 UTSW 2 85918136 missense probably damaging 1.00
R5960:Olfr1025-ps1 UTSW 2 85918725 missense probably benign 0.07
R6807:Olfr1025-ps1 UTSW 2 85918038 missense possibly damaging 0.48
R7320:Olfr1025-ps1 UTSW 2 85918374 missense probably benign 0.00
V1662:Olfr1025-ps1 UTSW 2 85918594 missense probably benign 0.10
Predicted Primers PCR Primer
(F):5'- ATTTGCAAATGGCCTGGCAC -3'
(R):5'- GGTTCAACATCGGGCTTACAAAG -3'

Sequencing Primer
(F):5'- TGGCACAGACCATCCTGATG -3'
(R):5'- TCGGGCTTACAAAGATATAAAAGACC -3'
Posted On2016-09-01