Mutagenetix > Incidental Mutation

Incidental Mutation 'R5445:Zfp786'

427397

Institutional Source

Beutler Lab

Gene Symbol

Zfp786

Ensembl Gene

ENSMUSG00000051499

Gene Name

zinc finger protein 786

Synonyms

A730012O14Rik

MMRRC Submission

043010-MU

Accession Numbers

Genbank: NM_177882; MGI: 3026883

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R5445 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

47819266-47830867 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 47819685 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 773 (E773G)

Ref Sequence

ENSEMBL: ENSMUSP00000057495 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058844]

AlphaFold

Q8BV42

Predicted Effect

probably damaging
Transcript: ENSMUST00000058844
AA Change: E773G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000057495
Gene: ENSMUSG00000051499
AA Change: E773G

Domain	Start	End	E-Value	Type
KRAB	9	69	2.6e-22	SMART
low complexity region	156	164	N/A	INTRINSIC
ZnF_C2H2	240	262	5.34e-1	SMART
ZnF_C2H2	268	295	1.41e2	SMART
ZnF_C2H2	420	440	1.21e2	SMART
ZnF_C2H2	448	470	3.52e-1	SMART
ZnF_C2H2	476	498	1.89e-1	SMART
ZnF_C2H2	504	526	1.69e-3	SMART
ZnF_C2H2	532	554	5.59e-4	SMART
ZnF_C2H2	560	582	4.79e-3	SMART
ZnF_C2H2	588	610	3.69e-4	SMART
ZnF_C2H2	616	638	3.63e-3	SMART
ZnF_C2H2	644	665	5.92e0	SMART
ZnF_C2H2	671	693	2.57e-3	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	1.45e-2	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000080603

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143979

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A430105I19Rik	T	C	2: 118,759,586	D259G	probably damaging	Het
Abcg5	A	T	17: 84,671,129	D300E	probably damaging	Het
Apbb1ip	T	C	2: 22,835,948	V244A	possibly damaging	Het
Arhgap32	T	C	9: 32,248,382	S232P	probably benign	Het
Atf7ip	G	A	6: 136,587,257	V833M	probably damaging	Het
Casp7	A	G	19: 56,433,338		probably null	Het
Celsr2	T	A	3: 108,392,658	E2911D	probably benign	Het
Cep350	T	C	1: 155,894,723	D1807G	probably benign	Het
Chrd	A	G	16: 20,738,910	T753A	possibly damaging	Het
Clasp2	A	G	9: 113,903,946	D971G	probably damaging	Het
Cnnm2	A	G	19: 46,877,288	T772A	possibly damaging	Het
Cntn1	A	T	15: 92,295,077	N687Y	probably damaging	Het
Col6a3	G	A	1: 90,782,039	R1812*	probably null	Het
Dsc2	T	C	18: 20,035,303	I700V	possibly damaging	Het
Fam71d	G	A	12: 78,715,116	E185K	probably damaging	Het
Flt3	G	A	5: 147,355,095	Q540*	probably null	Het
Fmo4	T	A	1: 162,805,273	I170F	probably benign	Het
Fra10ac1	T	A	19: 38,219,462	D72V	possibly damaging	Het
Gemin6	T	G	17: 80,227,749	V46G	probably damaging	Het
Gm13023	T	A	4: 143,795,137	V441E	possibly damaging	Het
Gm2381	T	G	7: 42,820,001	H233P	probably damaging	Het
Gm5148	T	A	3: 37,714,846	Q75L	probably damaging	Het
Gm8369	T	C	19: 11,504,806	V27A	possibly damaging	Het
Gpr157	T	C	4: 150,102,368	S318P	probably benign	Het
Hectd4	G	A	5: 121,266,274	V405M	probably benign	Het
Hemgn	T	C	4: 46,400,738	R41G	probably benign	Het
Hhipl1	T	A	12: 108,328,208	L791Q	probably damaging	Het
Hjurp	T	G	1: 88,266,316	K290T	probably benign	Het
Ifi207	T	C	1: 173,727,797	E773G	probably damaging	Het
Kcnh6	T	C	11: 106,023,859	Y697H	probably damaging	Het
Lonrf2	T	C	1: 38,807,153	T313A	probably benign	Het
Lrba	G	T	3: 86,368,595	V1757L	probably benign	Het
Lrrc24	T	C	15: 76,716,106	T278A	probably benign	Het
Ltbp2	T	C	12: 84,809,654	I679V	probably null	Het
Mapk4	G	T	18: 73,931,002	T383K	probably benign	Het
Mdn1	C	T	4: 32,723,690	P2542L	probably damaging	Het
Mia3	A	G	1: 183,336,022	V208A	probably benign	Het
Myo15	C	A	11: 60,520,777	C3234*	probably null	Het
Nlrp1b	G	T	11: 71,217,875	Q267K	probably benign	Het
Nphp3	A	G	9: 104,004,723	K37E	probably damaging	Het
Nwd2	T	C	5: 63,805,338	M755T	probably damaging	Het
Olfr482	A	G	7: 108,094,742	V276A	possibly damaging	Het
Olfr655	A	G	7: 104,596,821	F120S	probably damaging	Het
Olfr819	T	A	10: 129,966,289	H137L	probably benign	Het
Pdlim5	C	T	3: 142,352,734	R83K	probably null	Het
Plekha5	A	T	6: 140,552,733	R173*	probably null	Het
Rbms3	T	A	9: 117,251,785	D6V	possibly damaging	Het
Rhoq	A	T	17: 86,964,327	Y57F	probably benign	Het
Rrm1	A	T	7: 102,451,023	T204S	possibly damaging	Het
Slf1	A	T	13: 77,091,204	I447N	probably benign	Het
Smarcc2	T	A	10: 128,488,074		probably benign	Het
Spdye4c	C	T	2: 128,596,564	Q281*	probably null	Het
Tert	T	A	13: 73,644,284	M890K	probably benign	Het
Tln1	C	A	4: 43,543,905	R1198L	probably benign	Het
Tmco4	A	G	4: 139,020,867	M253V	probably damaging	Het
Usp19	G	T	9: 108,497,920	V782F	possibly damaging	Het
Usp33	T	A	3: 152,374,623	S464T	probably damaging	Het
Usp47	A	T	7: 112,074,721	Y397F	probably damaging	Het
Vmn1r12	A	G	6: 57,159,481	T144A	probably benign	Het
Vmn2r90	A	T	17: 17,734,124	H850L	probably benign	Het
Wdr66	C	T	5: 123,287,177	T294M	probably damaging	Het
Zfhx3	A	T	8: 108,956,210	Q3427L	unknown	Het
Zfp236	G	T	18: 82,682,156	Q63K	probably benign	Het
Zfp7	T	A	15: 76,890,854	C365*	probably null	Het

Other mutations in Zfp786

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Zfp786	APN	6	47819671	makesense	probably null
IGL02442:Zfp786	APN	6	47821367	missense	probably benign	0.00
IGL02674:Zfp786	APN	6	47820493	missense	probably benign	0.22
IGL02814:Zfp786	APN	6	47819841	missense	probably damaging	1.00
IGL03072:Zfp786	APN	6	47821243	missense	probably benign	0.01
IGL03294:Zfp786	APN	6	47821324	nonsense	probably null
IGL03393:Zfp786	APN	6	47821524	missense	possibly damaging	0.88
3-1:Zfp786	UTSW	6	47820445	missense	probably damaging	1.00
PIT4581001:Zfp786	UTSW	6	47819822	missense	probably damaging	1.00
R1071:Zfp786	UTSW	6	47821305	missense	possibly damaging	0.91
R1426:Zfp786	UTSW	6	47825079	missense	probably benign	0.17
R1976:Zfp786	UTSW	6	47819757	missense	probably damaging	1.00
R2114:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2115:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2117:Zfp786	UTSW	6	47826997	missense	probably damaging	1.00
R2176:Zfp786	UTSW	6	47820971	missense	possibly damaging	0.65
R2304:Zfp786	UTSW	6	47820699	missense	probably damaging	1.00
R3110:Zfp786	UTSW	6	47820226	missense	probably damaging	1.00
R3112:Zfp786	UTSW	6	47820226	missense	probably damaging	1.00
R3623:Zfp786	UTSW	6	47821423	missense	probably benign	0.00
R4654:Zfp786	UTSW	6	47820934	missense	probably benign	0.01
R4741:Zfp786	UTSW	6	47820691	missense	probably damaging	1.00
R4927:Zfp786	UTSW	6	47820153	missense	probably benign	0.43
R4936:Zfp786	UTSW	6	47821268	nonsense	probably null
R5084:Zfp786	UTSW	6	47820019	missense	probably benign	0.00
R6662:Zfp786	UTSW	6	47826986	missense	probably damaging	1.00
R7290:Zfp786	UTSW	6	47819995	missense	probably damaging	1.00
R7561:Zfp786	UTSW	6	47819733	missense	probably benign	0.02
R8038:Zfp786	UTSW	6	47821254	missense	probably benign	0.01
R8090:Zfp786	UTSW	6	47820009	missense	probably damaging	1.00
R8250:Zfp786	UTSW	6	47820795	missense	possibly damaging	0.85
R8438:Zfp786	UTSW	6	47820000	missense	probably damaging	1.00
R8516:Zfp786	UTSW	6	47820543	missense	probably damaging	1.00
R8868:Zfp786	UTSW	6	47825081	missense	probably damaging	0.99
R9596:Zfp786	UTSW	6	47820472	nonsense	probably null
R9788:Zfp786	UTSW	6	47820882	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTGCTTTTGGGTGAAATGCAC -3'
(R):5'- AAAAGGGTCACATGCTCAGG -3'

Sequencing Primer
(F):5'- ACCTCTTTTTACCAAGGGAGAGG -3'
(R):5'- TCAGGCACCAGCGCATC -3'

Posted On

2016-09-01