Mutagenetix > Incidental Mutation

Incidental Mutation 'R5445:Mapk4'

427434

Institutional Source

Beutler Lab

Gene Symbol

Mapk4

Ensembl Gene

ENSMUSG00000024558

Gene Name

mitogen-activated protein kinase 4

Synonyms

p63Mapk, A330097D03Rik, Erk3-related

MMRRC Submission

043010-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5445 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

74061557-74198430 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 74064073 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 383 (T383K)

Ref Sequence

ENSEMBL: ENSMUSP00000089462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091851] [ENSMUST00000159162] [ENSMUST00000162863]

AlphaFold

Q6P5G0

Predicted Effect

probably benign
Transcript: ENSMUST00000091851
AA Change: T383K

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000089462
Gene: ENSMUSG00000024558
AA Change: T383K

Domain	Start	End	E-Value	Type
S_TKc	20	312	3.81e-79	SMART
low complexity region	462	478	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000159162

SMART Domains

Protein: ENSMUSP00000123922
Gene: ENSMUSG00000024558

Domain	Start	End	E-Value	Type
Pfam:Pkinase	20	232	2.3e-58	PFAM
Pfam:Pkinase_Tyr	22	232	5.5e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000162863

SMART Domains

Protein: ENSMUSP00000124408
Gene: ENSMUSG00000024558

Domain	Start	End	E-Value	Type
Pfam:Pkinase	20	192	1.7e-41	PFAM
Pfam:Pkinase_Tyr	22	189	5.9e-25	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
PHENOTYPE: Mice homozygous for a knock-out allele are viabel and fertile. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg5	A	T	17: 84,978,557 (GRCm39)	D300E	probably damaging	Het
Apbb1ip	T	C	2: 22,725,960 (GRCm39)	V244A	possibly damaging	Het
Arhgap32	T	C	9: 32,159,678 (GRCm39)	S232P	probably benign	Het
Atf7ip	G	A	6: 136,564,255 (GRCm39)	V833M	probably damaging	Het
Casp7	A	G	19: 56,421,770 (GRCm39)		probably null	Het
Ccdc9b	T	C	2: 118,590,067 (GRCm39)	D259G	probably damaging	Het
Celsr2	T	A	3: 108,299,974 (GRCm39)	E2911D	probably benign	Het
Cep350	T	C	1: 155,770,469 (GRCm39)	D1807G	probably benign	Het
Cfap251	C	T	5: 123,425,240 (GRCm39)	T294M	probably damaging	Het
Chrd	A	G	16: 20,557,660 (GRCm39)	T753A	possibly damaging	Het
Clasp2	A	G	9: 113,733,014 (GRCm39)	D971G	probably damaging	Het
Cnnm2	A	G	19: 46,865,727 (GRCm39)	T772A	possibly damaging	Het
Cntn1	A	T	15: 92,192,958 (GRCm39)	N687Y	probably damaging	Het
Col6a3	G	A	1: 90,709,761 (GRCm39)	R1812*	probably null	Het
Dsc2	T	C	18: 20,168,360 (GRCm39)	I700V	possibly damaging	Het
Flt3	G	A	5: 147,291,905 (GRCm39)	Q540*	probably null	Het
Fmo4	T	A	1: 162,632,842 (GRCm39)	I170F	probably benign	Het
Fra10ac1	T	A	19: 38,207,910 (GRCm39)	D72V	possibly damaging	Het
Garin2	G	A	12: 78,761,890 (GRCm39)	E185K	probably damaging	Het
Gemin6	T	G	17: 80,535,178 (GRCm39)	V46G	probably damaging	Het
Gm2381	T	G	7: 42,469,425 (GRCm39)	H233P	probably damaging	Het
Gm5148	T	A	3: 37,768,995 (GRCm39)	Q75L	probably damaging	Het
Gm8369	T	C	19: 11,482,170 (GRCm39)	V27A	possibly damaging	Het
Gpr157	T	C	4: 150,186,825 (GRCm39)	S318P	probably benign	Het
Hectd4	G	A	5: 121,404,337 (GRCm39)	V405M	probably benign	Het
Hemgn	T	C	4: 46,400,738 (GRCm39)	R41G	probably benign	Het
Hhipl1	T	A	12: 108,294,467 (GRCm39)	L791Q	probably damaging	Het
Hjurp	T	G	1: 88,194,038 (GRCm39)	K290T	probably benign	Het
Ifi207	T	C	1: 173,555,363 (GRCm39)	E773G	probably damaging	Het
Kcnh6	T	C	11: 105,914,685 (GRCm39)	Y697H	probably damaging	Het
Lonrf2	T	C	1: 38,846,234 (GRCm39)	T313A	probably benign	Het
Lrba	G	T	3: 86,275,902 (GRCm39)	V1757L	probably benign	Het
Lrrc24	T	C	15: 76,600,306 (GRCm39)	T278A	probably benign	Het
Ltbp2	T	C	12: 84,856,428 (GRCm39)	I679V	probably null	Het
Mdn1	C	T	4: 32,723,690 (GRCm39)	P2542L	probably damaging	Het
Mia3	A	G	1: 183,117,471 (GRCm39)	V208A	probably benign	Het
Myo15a	C	A	11: 60,411,603 (GRCm39)	C3234*	probably null	Het
Nlrp1b	G	T	11: 71,108,701 (GRCm39)	Q267K	probably benign	Het
Nphp3	A	G	9: 103,881,922 (GRCm39)	K37E	probably damaging	Het
Nwd2	T	C	5: 63,962,681 (GRCm39)	M755T	probably damaging	Het
Or10u4	T	A	10: 129,802,158 (GRCm39)	H137L	probably benign	Het
Or52ac1	A	G	7: 104,246,028 (GRCm39)	F120S	probably damaging	Het
Or5p58	A	G	7: 107,693,949 (GRCm39)	V276A	possibly damaging	Het
Pdlim5	C	T	3: 142,058,495 (GRCm39)	R83K	probably null	Het
Plekha5	A	T	6: 140,498,459 (GRCm39)	R173*	probably null	Het
Pramel25	T	A	4: 143,521,707 (GRCm39)	V441E	possibly damaging	Het
Rbms3	T	A	9: 117,080,853 (GRCm39)	D6V	possibly damaging	Het
Rhoq	A	T	17: 87,271,755 (GRCm39)	Y57F	probably benign	Het
Rrm1	A	T	7: 102,100,230 (GRCm39)	T204S	possibly damaging	Het
Slf1	A	T	13: 77,239,323 (GRCm39)	I447N	probably benign	Het
Smarcc2	T	A	10: 128,323,943 (GRCm39)		probably benign	Het
Spdye4c	C	T	2: 128,438,484 (GRCm39)	Q281*	probably null	Het
Tert	T	A	13: 73,792,403 (GRCm39)	M890K	probably benign	Het
Tln1	C	A	4: 43,543,905 (GRCm39)	R1198L	probably benign	Het
Tmco4	A	G	4: 138,748,178 (GRCm39)	M253V	probably damaging	Het
Usp19	G	T	9: 108,375,119 (GRCm39)	V782F	possibly damaging	Het
Usp33	T	A	3: 152,080,260 (GRCm39)	S464T	probably damaging	Het
Usp47	A	T	7: 111,673,928 (GRCm39)	Y397F	probably damaging	Het
Vmn1r12	A	G	6: 57,136,466 (GRCm39)	T144A	probably benign	Het
Vmn2r90	A	T	17: 17,954,386 (GRCm39)	H850L	probably benign	Het
Zfhx3	A	T	8: 109,682,842 (GRCm39)	Q3427L	unknown	Het
Zfp236	G	T	18: 82,700,281 (GRCm39)	Q63K	probably benign	Het
Zfp7	T	A	15: 76,775,054 (GRCm39)	C365*	probably null	Het
Zfp786	T	C	6: 47,796,619 (GRCm39)	E773G	probably damaging	Het

Other mutations in Mapk4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02396:Mapk4	APN	18	74,067,068 (GRCm39)	splice site	probably null
IGL02621:Mapk4	APN	18	74,103,346 (GRCm39)	missense	probably damaging	1.00
IGL03068:Mapk4	APN	18	74,103,415 (GRCm39)	missense	probably damaging	1.00
R0041:Mapk4	UTSW	18	74,068,109 (GRCm39)	missense	probably damaging	1.00
R0519:Mapk4	UTSW	18	74,103,392 (GRCm39)	missense	probably damaging	1.00
R0636:Mapk4	UTSW	18	74,063,525 (GRCm39)	missense	probably benign
R0918:Mapk4	UTSW	18	74,103,408 (GRCm39)	missense	probably damaging	1.00
R1654:Mapk4	UTSW	18	74,064,010 (GRCm39)	missense	probably damaging	1.00
R2913:Mapk4	UTSW	18	74,068,236 (GRCm39)	missense	probably benign	0.34
R2914:Mapk4	UTSW	18	74,068,236 (GRCm39)	missense	probably benign	0.34
R4089:Mapk4	UTSW	18	74,063,530 (GRCm39)	missense	probably damaging	1.00
R4414:Mapk4	UTSW	18	74,063,609 (GRCm39)	missense	possibly damaging	0.76
R4487:Mapk4	UTSW	18	74,064,046 (GRCm39)	missense	probably damaging	1.00
R4792:Mapk4	UTSW	18	74,070,321 (GRCm39)	missense	probably damaging	0.98
R5597:Mapk4	UTSW	18	74,070,341 (GRCm39)	missense	probably benign	0.12
R5654:Mapk4	UTSW	18	74,103,365 (GRCm39)	missense	probably damaging	1.00
R6700:Mapk4	UTSW	18	74,063,882 (GRCm39)	missense	probably damaging	1.00
R6793:Mapk4	UTSW	18	74,063,539 (GRCm39)	missense	probably damaging	1.00
R7200:Mapk4	UTSW	18	74,063,990 (GRCm39)	missense	possibly damaging	0.92
R7335:Mapk4	UTSW	18	74,070,338 (GRCm39)	missense	possibly damaging	0.75
R8010:Mapk4	UTSW	18	74,063,647 (GRCm39)	missense	probably benign	0.04
R8269:Mapk4	UTSW	18	74,063,622 (GRCm39)	missense	probably damaging	0.99
R8736:Mapk4	UTSW	18	74,103,396 (GRCm39)	missense	probably benign	0.01
Z1176:Mapk4	UTSW	18	74,070,255 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCACAGTAGCTTGTCCAGGTAG -3'
(R):5'- GGATGAGAGAACCTGCCCTTTAG -3'

Sequencing Primer
(F):5'- TCCAGGTAGGATGGAGACCC -3'
(R):5'- CTAGCAGACTTGTGAGTAAAGGCC -3'

Posted On

2016-09-01