Incidental Mutation 'R5440:Zfp770'
ID428570
Institutional Source Beutler Lab
Gene Symbol Zfp770
Ensembl Gene ENSMUSG00000040321
Gene Namezinc finger protein 770
Synonyms6430601A21Rik
MMRRC Submission 043005-MU
Accession Numbers

Ncbi RefSeq: NM_175466.4; MGI: 2445100

Is this an essential gene? Probably non essential (E-score: 0.222) question?
Stock #R5440 (G1)
Quality Score225
Status Not validated
Chromosome2
Chromosomal Location114193461-114201469 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 114196115 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 491 (D491G)
Ref Sequence ENSEMBL: ENSMUSP00000052194 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000050668]
Predicted Effect probably benign
Transcript: ENSMUST00000050668
AA Change: D491G

PolyPhen 2 Score 0.421 (Sensitivity: 0.89; Specificity: 0.90)
SMART Domains Protein: ENSMUSP00000052194
Gene: ENSMUSG00000040321
AA Change: D491G

DomainStartEndE-ValueType
ZnF_C2H2 31 53 1.18e-2 SMART
ZnF_C2H2 59 81 4.11e-2 SMART
ZnF_C2H2 85 107 3.58e-2 SMART
ZnF_C2H2 164 186 2.09e-3 SMART
ZnF_C2H2 192 214 3.58e-2 SMART
ZnF_C2H2 220 242 3.29e-1 SMART
ZnF_C2H2 298 318 1.93e2 SMART
low complexity region 341 354 N/A INTRINSIC
ZnF_C2H2 485 507 5.9e-3 SMART
ZnF_C2H2 513 535 1.82e-3 SMART
low complexity region 576 595 N/A INTRINSIC
ZnF_C2H2 640 662 9.58e-3 SMART
ZnF_C2H2 668 690 7.37e-4 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123562
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.3%
  • 20x: 95.3%
Validation Efficiency
Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Apc G T 18: 34,221,160 probably benign Het
Apol11b T A 15: 77,635,593 K96* probably null Het
Arhgap35 C T 7: 16,562,924 G739S probably damaging Het
Atp6v0a4 A C 6: 38,092,817 F47V probably damaging Het
Bcl9 G A 3: 97,210,565 P271L probably benign Het
Cd109 T C 9: 78,680,164 probably null Het
Col12a1 T A 9: 79,614,363 I2771F probably benign Het
Col6a1 A G 10: 76,723,454 V116A probably damaging Het
Cpsf2 C G 12: 101,996,879 L401V probably benign Het
Cwf19l2 G A 9: 3,475,549 E829K probably damaging Het
D7Ertd443e G A 7: 134,349,275 T223I probably damaging Het
Dtx4 G A 19: 12,492,317 R149C probably damaging Het
Fam186b C T 15: 99,273,853 A838T possibly damaging Het
Fzd4 G T 7: 89,408,118 E458* probably null Het
Gm17669 C T 18: 67,562,456 P24S possibly damaging Het
Grin2b C T 6: 135,736,306 G859D probably damaging Het
Gucy2e T C 11: 69,223,646 Y1019C probably damaging Het
Gzme T A 14: 56,118,453 N134I possibly damaging Het
Havcr1 A T 11: 46,752,370 Y39F probably damaging Het
Hint3 A T 10: 30,618,351 M1K probably null Het
Hspa4l A G 3: 40,781,576 K543R probably damaging Het
Ifna9 A G 4: 88,591,811 probably null Het
Itgb4 C T 11: 115,984,157 R447W probably benign Het
Lipo2 A G 19: 33,720,858 I373T probably benign Het
Myo5c T A 9: 75,258,125 I405N possibly damaging Het
Olfr517 T C 7: 108,868,626 H176R probably damaging Het
P2rx1 A G 11: 73,008,503 M108V probably benign Het
Pcsk2 T A 2: 143,546,543 V18E probably benign Het
Pigr A T 1: 130,849,622 probably null Het
Pkp1 A T 1: 135,882,492 C447S probably benign Het
Prom1 T A 5: 44,058,646 I96F probably benign Het
Prpf4b T C 13: 34,884,093 probably benign Het
Ropn1 C A 16: 34,671,172 D102E probably benign Het
Slc35e2 C T 4: 155,610,026 P10L probably benign Het
Sphk1 T G 11: 116,534,888 V17G possibly damaging Het
Syngr1 A G 15: 80,098,018 N2S probably benign Het
Syt9 T G 7: 107,502,123 S359A possibly damaging Het
Terb1 T C 8: 104,488,499 I282V probably damaging Het
Ttn T A 2: 76,754,824 I22042F probably damaging Het
Ttn A T 2: 76,909,256 D3646E probably benign Het
Ube2d1 A G 10: 71,255,852 W141R probably damaging Het
Vmn2r41 T A 7: 8,138,363 I701F probably damaging Het
Other mutations in Zfp770
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00090:Zfp770 APN 2 114195932 missense probably benign 0.20
IGL00478:Zfp770 APN 2 114197465 missense probably damaging 1.00
IGL01539:Zfp770 APN 2 114197093 missense probably damaging 0.99
IGL01778:Zfp770 APN 2 114196238 missense probably damaging 0.96
IGL02596:Zfp770 APN 2 114195827 missense probably benign 0.11
IGL03227:Zfp770 APN 2 114197089 nonsense probably null
R0057:Zfp770 UTSW 2 114197232 nonsense probably null
R0057:Zfp770 UTSW 2 114197232 nonsense probably null
R1081:Zfp770 UTSW 2 114197127 missense probably damaging 1.00
R1446:Zfp770 UTSW 2 114197033 missense probably damaging 0.99
R4477:Zfp770 UTSW 2 114196884 missense probably damaging 1.00
R4597:Zfp770 UTSW 2 114196770 missense possibly damaging 0.46
R4964:Zfp770 UTSW 2 114197387 missense probably benign 0.24
R4966:Zfp770 UTSW 2 114197387 missense probably benign 0.24
R5259:Zfp770 UTSW 2 114197193 missense probably benign 0.00
R5910:Zfp770 UTSW 2 114196232 nonsense probably null
R5941:Zfp770 UTSW 2 114197546 missense possibly damaging 0.83
R6074:Zfp770 UTSW 2 114196389 missense possibly damaging 0.68
R6341:Zfp770 UTSW 2 114196759 missense probably benign 0.14
R7181:Zfp770 UTSW 2 114197391 missense probably damaging 1.00
R7288:Zfp770 UTSW 2 114195661 nonsense probably null
R7935:Zfp770 UTSW 2 114196824 missense probably benign 0.00
R8119:Zfp770 UTSW 2 114197027 missense probably damaging 1.00
R8304:Zfp770 UTSW 2 114197410 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGTTCACATTTCCAAAGTCCACTC -3'
(R):5'- ATTCCAGAGCCAAAATACGGG -3'

Sequencing Primer
(F):5'- CTCGCCAAAAAGAACTGTAAGG -3'
(R):5'- GAGTTTGCAAGGCATCCTTAC -3'
Posted On2016-09-01