Mutagenetix > Incidental Mutation

Incidental Mutation 'R5372:Ifna15'

428772

Institutional Source

Beutler Lab

Gene Symbol

Ifna15

Ensembl Gene

ENSMUSG00000096011

Gene Name

interferon alpha 15

Synonyms

Ifnaa, Gm12597

MMRRC Submission

042948-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R5372 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88557673-88558245 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 88558101 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Proline to Serine at position 49 (P49S)

Ref Sequence

ENSEMBL: ENSMUSP00000099873 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102809]

AlphaFold

Q61718

Predicted Effect

probably damaging
Transcript: ENSMUST00000102809
AA Change: P49S

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000099873
Gene: ENSMUSG00000096011
AA Change: P49S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	2.97e-68	SMART

Meta Mutation Damage Score

0.2541

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

96% (89/93)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 122,055,339 (GRCm38)	D108E	probably damaging	Het
Abhd12b	A	G	12: 70,181,026 (GRCm38)	D194G	probably damaging	Het
Adck5	A	G	15: 76,594,507 (GRCm38)		probably benign	Het
Adgrb3	C	T	1: 25,128,859 (GRCm38)	V792I	probably benign	Het
Anxa8	G	A	14: 34,093,911 (GRCm38)	V174M	probably damaging	Het
Apol9b	A	T	15: 77,735,720 (GRCm38)	R239W	probably benign	Het
Arhgap26	A	G	18: 38,642,456 (GRCm38)		noncoding transcript	Het
Atrnl1	A	G	19: 57,755,536 (GRCm38)	Y1190C	probably benign	Het
Brinp3	T	G	1: 146,831,726 (GRCm38)	L376R	probably damaging	Het
Btbd2	A	T	10: 80,648,641 (GRCm38)	M132K	probably damaging	Het
C130073F10Rik	A	T	4: 101,890,487 (GRCm38)	I115K	probably damaging	Het
C1ra	T	A	6: 124,521,625 (GRCm38)	Y426N	probably damaging	Het
Cacna1b	A	T	2: 24,733,959 (GRCm38)	V203E	probably damaging	Het
Catsperg1	T	A	7: 29,210,712 (GRCm38)	D68V	probably benign	Het
Ccdc158	A	C	5: 92,632,560 (GRCm38)	S885A	possibly damaging	Het
Cdc42bpa	T	C	1: 180,064,979 (GRCm38)	V236A	probably damaging	Het
Cdca7	A	T	2: 72,482,449 (GRCm38)	E176D	probably damaging	Het
Cdk17	A	G	10: 93,226,039 (GRCm38)	D211G	probably benign	Het
Clca3a2	A	C	3: 144,797,525 (GRCm38)	M888R	probably benign	Het
Clcn7	T	A	17: 25,157,179 (GRCm38)	M568K	possibly damaging	Het
Clip1	G	T	5: 123,630,240 (GRCm38)	N811K	probably benign	Het
Col12a1	T	A	9: 79,678,366 (GRCm38)	Y1243F	probably damaging	Het
Dctn1	T	A	6: 83,190,210 (GRCm38)	D315E	probably damaging	Het
Dgcr2	T	C	16: 17,872,644 (GRCm38)	T41A	probably benign	Het
Dync2h1	G	A	9: 7,176,962 (GRCm38)		probably benign	Het
Ep300	A	G	15: 81,636,830 (GRCm38)	I1264V	unknown	Het
Fam167b	A	T	4: 129,578,299 (GRCm38)	L26Q	possibly damaging	Het
Fam178b	T	A	1: 36,564,848 (GRCm38)	I457F	possibly damaging	Het
Fgd4	A	T	16: 16,484,291 (GRCm38)	N133K	probably benign	Het
Fndc1	C	G	17: 7,765,210 (GRCm38)	V1295L	unknown	Het
Gad2	A	T	2: 22,690,243 (GRCm38)	D552V	possibly damaging	Het
Hars2	T	C	18: 36,790,481 (GRCm38)	Y361H	possibly damaging	Het
Heca	T	A	10: 17,915,139 (GRCm38)	S390C	probably damaging	Het
Hephl1	G	T	9: 15,097,899 (GRCm38)	Y132*	probably null	Het
Hormad1	T	A	3: 95,576,424 (GRCm38)	D182E	probably damaging	Het
Khsrp	T	C	17: 57,024,292 (GRCm38)	T429A	possibly damaging	Het
Magi2	A	G	5: 20,702,110 (GRCm38)	Q1094R	possibly damaging	Het
Map3k11	T	A	19: 5,690,962 (GRCm38)	I239K	probably damaging	Het
Mtus2	A	G	5: 148,313,412 (GRCm38)	T1319A	probably damaging	Het
Nup54	A	G	5: 92,417,857 (GRCm38)	I406T	probably damaging	Het
Nxpe2	T	C	9: 48,339,519 (GRCm38)	T43A	possibly damaging	Het
Nynrin	A	G	14: 55,868,491 (GRCm38)	E889G	probably benign	Het
Olfr1313	A	G	2: 112,072,109 (GRCm38)	I158T	probably benign	Het
Olfr160	T	C	9: 37,711,938 (GRCm38)	M114V	possibly damaging	Het
Olfr301	T	A	7: 86,412,968 (GRCm38)	I202N	possibly damaging	Het
Olfr663	A	G	7: 104,703,795 (GRCm38)	D76G	probably benign	Het
Opa1	A	C	16: 29,586,119 (GRCm38)	H45P	probably benign	Het
Otx1	C	A	11: 21,997,037 (GRCm38)	A91S	probably damaging	Het
Papola	A	G	12: 105,827,050 (GRCm38)	K543R	probably benign	Het
Plcxd3	G	A	15: 4,574,788 (GRCm38)	V293I	probably benign	Het
Polr2g	T	C	19: 8,797,303 (GRCm38)	Y72C	probably damaging	Het
Ppp1r21	A	G	17: 88,550,675 (GRCm38)	K205E	probably benign	Het
Ptpre	A	G	7: 135,653,940 (GRCm38)	K53E	possibly damaging	Het
Rasal3	T	C	17: 32,391,344 (GRCm38)	K990E	probably benign	Het
Rgs3	A	T	4: 62,652,697 (GRCm38)		probably benign	Het
Rhd	A	G	4: 134,884,632 (GRCm38)	T254A	possibly damaging	Het
Rufy4	T	C	1: 74,147,663 (GRCm38)	C537R	probably damaging	Het
Scube3	G	T	17: 28,152,482 (GRCm38)	C57F	probably damaging	Het
Sh2d5	A	G	4: 138,254,699 (GRCm38)	D57G	possibly damaging	Het
Slc12a6	T	A	2: 112,347,360 (GRCm38)	L608*	probably null	Het
Slk	A	T	19: 47,625,393 (GRCm38)	N896I	probably damaging	Het
Smc6	A	G	12: 11,282,430 (GRCm38)	D211G	probably damaging	Het
Sox6	A	T	7: 115,550,151 (GRCm38)	Y371*	probably null	Het
Srcap	G	A	7: 127,557,613 (GRCm38)		probably null	Het
Stard5	T	A	7: 83,633,220 (GRCm38)	D80E	probably damaging	Het
Supv3l1	C	T	10: 62,432,357 (GRCm38)	V570M	probably damaging	Het
Syt7	G	T	19: 10,426,621 (GRCm38)	V180L	probably damaging	Het
Tacc2	T	A	7: 130,623,260 (GRCm38)	H558Q	probably benign	Het
Tas2r120	T	A	6: 132,657,483 (GRCm38)	M176K	possibly damaging	Het
Tmem135	A	T	7: 89,165,174 (GRCm38)		probably null	Het
Trim35	T	C	14: 66,297,266 (GRCm38)	V66A	possibly damaging	Het
Tspan12	A	G	6: 21,772,699 (GRCm38)	S284P	probably benign	Het
Ttll3	A	T	6: 113,401,421 (GRCm38)	K257*	probably null	Het
Uggt1	C	A	1: 36,244,060 (GRCm38)		probably benign	Het
Vmn2r7	T	A	3: 64,716,324 (GRCm38)	I283F	probably damaging	Het
Wdfy2	T	G	14: 62,954,885 (GRCm38)	H363Q	probably damaging	Het
Wdr4	T	A	17: 31,510,580 (GRCm38)	K95N	probably damaging	Het
Zfp141	T	C	7: 42,477,196 (GRCm38)	N91S	possibly damaging	Het
Zfp383	C	T	7: 29,915,270 (GRCm38)	R317*	probably null	Het

Other mutations in Ifna15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01575:Ifna15	APN	4	88,557,807 (GRCm38)	missense	probably damaging	1.00
IGL01757:Ifna15	APN	4	88,558,085 (GRCm38)	missense	possibly damaging	0.79
IGL01824:Ifna15	APN	4	88,557,783 (GRCm38)	missense	probably benign	0.00
R3853:Ifna15	UTSW	4	88,557,809 (GRCm38)	missense	probably damaging	1.00
R4356:Ifna15	UTSW	4	88,557,842 (GRCm38)	missense	probably benign	0.09
R4357:Ifna15	UTSW	4	88,557,842 (GRCm38)	missense	probably benign	0.09
R4358:Ifna15	UTSW	4	88,557,842 (GRCm38)	missense	probably benign	0.09
R4359:Ifna15	UTSW	4	88,557,842 (GRCm38)	missense	probably benign	0.09
R5038:Ifna15	UTSW	4	88,558,029 (GRCm38)	missense	probably benign	0.03
R6036:Ifna15	UTSW	4	88,558,073 (GRCm38)	missense	possibly damaging	0.65
R6036:Ifna15	UTSW	4	88,558,073 (GRCm38)	missense	possibly damaging	0.65
R7347:Ifna15	UTSW	4	88,557,983 (GRCm38)	missense	probably damaging	1.00
R8097:Ifna15	UTSW	4	88,557,701 (GRCm38)	missense	probably benign	0.01
R8132:Ifna15	UTSW	4	88,557,683 (GRCm38)	missense	possibly damaging	0.60
R8824:Ifna15	UTSW	4	88,557,761 (GRCm38)	missense	probably damaging	1.00
R9349:Ifna15	UTSW	4	88,558,046 (GRCm38)	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- AGTCTAGGAGGGATGCATCC -3'
(R):5'- ACAGGATGGTCTTCAGAGAACC -3'

Sequencing Primer
(F):5'- CCAAGCAGCAGATGAGTCCTTTG -3'
(R):5'- TGGTCTTCAGAGAACCTAGAGG -3'

Posted On

2016-09-06