Mutagenetix > Incidental Mutation

Incidental Mutation 'R5446:Muc21'

429097

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

043011-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R5446 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

T to A at 35933395 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134221 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: T264S

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: T264S

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173759

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174534

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adar	A	G	3: 89,647,486 (GRCm39)	N155S	probably damaging	Het
Ak9	A	G	10: 41,296,505 (GRCm39)	D1417G	possibly damaging	Het
Ankrd52	G	T	10: 128,224,430 (GRCm39)	C736F	probably damaging	Het
Arhgap15	T	A	2: 43,718,772 (GRCm39)	H85Q	probably benign	Het
Bcat2	G	A	7: 45,234,569 (GRCm39)	R110H	possibly damaging	Het
Bscl2	A	G	19: 8,823,564 (GRCm39)	H4R	possibly damaging	Het
Cdh2	T	C	18: 16,779,684 (GRCm39)	I126V	probably damaging	Het
Cnbd2	A	C	2: 156,209,581 (GRCm39)	E508A	possibly damaging	Het
Crb2	T	C	2: 37,685,461 (GRCm39)	I1191T	probably benign	Het
Dync2h1	T	C	9: 7,144,217 (GRCm39)	T1075A	probably benign	Het
Edil3	A	C	13: 89,332,957 (GRCm39)	H371P	possibly damaging	Het
Gm5134	T	C	10: 75,831,670 (GRCm39)	S370P	probably damaging	Het
Helz	T	C	11: 107,523,030 (GRCm39)	V737A	probably damaging	Het
Jade2	A	G	11: 51,707,786 (GRCm39)	V809A	probably benign	Het
Klhl23	T	A	2: 69,654,582 (GRCm39)	C151S	probably damaging	Het
Krt75	T	C	15: 101,479,502 (GRCm39)	D276G	probably null	Het
Lipm	C	T	19: 34,095,287 (GRCm39)	A294V	possibly damaging	Het
Med13l	A	G	5: 118,880,462 (GRCm39)	N1185D	possibly damaging	Het
Mmp27	T	A	9: 7,573,516 (GRCm39)		probably benign	Het
Mroh2a	A	T	1: 88,182,687 (GRCm39)	N1205I	possibly damaging	Het
Npnt	A	G	3: 132,614,130 (GRCm39)	L191P	probably damaging	Het
Or4c10b	A	T	2: 89,711,893 (GRCm39)	H241L	probably damaging	Het
Paip2b	T	C	6: 83,791,844 (GRCm39)	I13V	probably benign	Het
Pcdhac2	T	A	18: 37,278,253 (GRCm39)	L411Q	probably damaging	Het
Plekhd1	A	G	12: 80,767,410 (GRCm39)	N266S	probably benign	Het
Pnpla8	A	G	12: 44,337,368 (GRCm39)	T454A	possibly damaging	Het
Prkcg	A	T	7: 3,378,780 (GRCm39)	Y675F	probably benign	Het
Pwwp2b	T	A	7: 138,835,066 (GRCm39)	I169N	probably damaging	Het
Rreb1	G	A	13: 38,082,473 (GRCm39)	R86H	possibly damaging	Het
Smok3c	A	C	5: 138,062,895 (GRCm39)	L127F	probably damaging	Het
St6galnac1	T	A	11: 116,657,095 (GRCm39)	M427L	probably benign	Het
Synm	T	C	7: 67,385,722 (GRCm39)	T205A	probably benign	Het
Tsbp1	T	C	17: 34,659,867 (GRCm39)		probably null	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Vmn2r112	A	G	17: 22,837,231 (GRCm39)	Y564C	probably damaging	Het
Vmn2r90	A	C	17: 17,932,464 (GRCm39)	T124P	probably damaging	Het
Vpreb1a	A	T	16: 16,686,554 (GRCm39)	V112E	probably damaging	Het
Zfp451	A	G	1: 33,816,609 (GRCm39)	L447S	probably damaging	Het
Zfp746	T	C	6: 48,041,107 (GRCm39)	T539A	probably damaging	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R2866:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5716:Muc21	UTSW	17	35,931,675 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R6940:Muc21	UTSW	17	35,934,118 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8464:Muc21	UTSW	17	35,933,098 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9353:Muc21	UTSW	17	35,930,545 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGAGGCAGTGCTGGATTCAG -3'
(R):5'- TGCCTCAGGATCTACACCTACC -3'

Sequencing Primer
(F):5'- CTGGATTCAGTGGTGGGCAG -3'
(R):5'- ATCCAGCACTGCCTCAGG -3'

Posted On

2016-09-06