Mutagenetix > Incidental Mutation

Incidental Mutation 'R5447:Stk10'

429139

Institutional Source

Beutler Lab

Gene Symbol

Stk10

Ensembl Gene

ENSMUSG00000020272

Gene Name

serine/threonine kinase 10

Synonyms

Gek1, Lok

MMRRC Submission

043012-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.154) question?

Stock #

R5447 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

32533305-32624587 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 32604166 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 618 (Q618*)

Ref Sequence

ENSEMBL: ENSMUSP00000099885 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000102821]

AlphaFold

O55098

Predicted Effect

probably null
Transcript: ENSMUST00000102821
AA Change: Q618*

SMART Domains

Protein: ENSMUSP00000099885
Gene: ENSMUSG00000020272
AA Change: Q618*

Domain	Start	End	E-Value	Type
S_TKc	36	294	8.66e-92	SMART
low complexity region	316	334	N/A	INTRINSIC
low complexity region	544	577	N/A	INTRINSIC
Pfam:PKK	586	724	1.9e-41	PFAM
Pfam:PKK	754	894	2.2e-37	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

97% (71/73)

MGI Phenotype

FUNCTION: This gene encodes a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. Mice deficient for this gene product are viable, but exhibit altered integrin-mediated lymphocyte adhesion characteristics. The orthologous gene product in humans can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Targeted disruption of this gene results in enhanced cell adhesion in mitogen-stimulated T cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5830473C10Rik	C	A	5: 90,584,310	A458E	probably damaging	Het
Abcb5	T	C	12: 118,927,326	I479V	probably damaging	Het
Adam30	A	G	3: 98,161,343	D164G	probably benign	Het
Adgrl3	T	A	5: 81,465,341		probably benign	Het
Adrb1	T	C	19: 56,723,087	I239T	probably benign	Het
B4galnt3	T	C	6: 120,215,057	T572A	probably benign	Het
Baz2b	T	C	2: 59,913,988	E1391G	probably damaging	Het
BC016579	A	G	16: 45,648,889	V72A	probably benign	Het
Btnl10	A	T	11: 58,922,318	I258F	probably benign	Het
Cdh5	A	T	8: 104,129,362	D309V	probably damaging	Het
Cdhr2	A	G	13: 54,733,250	D1042G	probably damaging	Het
Clk2	G	T	3: 89,167,191	V53F	possibly damaging	Het
Cyfip2	T	C	11: 46,291,586	D15G	possibly damaging	Het
Dip2b	C	T	15: 100,211,986	R1451C	probably damaging	Het
Dmbt1	A	G	7: 131,119,511	Y1836C	probably damaging	Het
Dysf	T	C	6: 84,195,263	F1905L	probably damaging	Het
E130114P18Rik	A	G	4: 97,690,718	S7P	unknown	Het
Fam110a	T	C	2: 151,970,709	E47G	probably damaging	Het
Gemin6	T	G	17: 80,227,749	V46G	probably damaging	Het
H2-Ke6	A	T	17: 34,026,912	V202D	probably damaging	Het
Helb	T	A	10: 120,102,901	D556V	possibly damaging	Het
Hoxd4	A	G	2: 74,727,343	E22G	probably damaging	Het
Il1rl2	T	G	1: 40,329,156	I162R	probably damaging	Het
Lhfpl5	A	G	17: 28,576,097	T33A	probably damaging	Het
Mapk8ip3	G	A	17: 24,899,189	A1283V	probably benign	Het
Mettl13	A	G	1: 162,535,880	V227A	probably benign	Het
Mmgt2	T	A	11: 62,664,998	C57*	probably null	Het
Muc4	G	C	16: 32,753,919	R1265P	probably benign	Het
Mylk2	T	C	2: 152,912,510	S175P	probably damaging	Het
Neu4	C	T	1: 94,022,418	T33M	probably damaging	Het
Nfs1	C	T	2: 156,142,136	R107H	probably benign	Het
Nfxl1	C	T	5: 72,529,169	R563Q	probably benign	Het
Nid1	A	G	13: 13,437,910	D70G	probably benign	Het
Nup160	C	A	2: 90,725,615	Q1220K	possibly damaging	Het
Olfr175-ps1	A	T	16: 58,824,483	C75*	probably null	Het
Olfr381	A	G	11: 73,486,176	V216A	probably benign	Het
Olfr51	G	A	11: 51,007,343	V124M	possibly damaging	Het
Olfr605	A	C	7: 103,442,940	M61R	probably damaging	Het
Pdgfrb	T	A	18: 61,068,108	V422E	probably damaging	Het
Pear1	G	A	3: 87,759,142	R85C	probably damaging	Het
Pkhd1	T	A	1: 20,239,385	M2780L	probably benign	Het
Ppp4r4	T	C	12: 103,584,151	V62A	possibly damaging	Het
Prol1	C	T	5: 88,328,266	P172S	unknown	Het
Proz	A	G	8: 13,072,578	I231V	probably benign	Het
Ptch1	T	G	13: 63,527,245	M718L	probably benign	Het
Ptprs	A	G	17: 56,429,128	C102R	possibly damaging	Het
Robo2	A	T	16: 73,973,766	Y490*	probably null	Het
Rptor	G	A	11: 119,843,713	G514D	probably damaging	Het
Scara5	CG	C	14: 65,759,662		probably null	Het
Skint6	T	A	4: 113,105,909	S442C	probably benign	Het
Snw1	T	C	12: 87,455,715	E303G	probably benign	Het
Sp110	C	G	1: 85,589,118	E219D	probably damaging	Het
Stam2	G	A	2: 52,736,293		probably benign	Het
Tmc3	A	T	7: 83,622,361	E907V	possibly damaging	Het
Ttn	A	T	2: 76,811,243	L5176Q	possibly damaging	Het
Ttn	T	A	2: 76,899,107		probably benign	Het
Vps39	T	C	2: 120,352,932	D19G	probably benign	Het
Zan	T	C	5: 137,472,191	S229G	probably damaging	Het
Zfp141	A	T	7: 42,475,559	C496*	probably null	Het
Zgrf1	T	C	3: 127,563,119	S665P	possibly damaging	Het

Other mutations in Stk10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01105:Stk10	APN	11	32577740	missense	probably benign	0.33
IGL01285:Stk10	APN	11	32610653	missense	possibly damaging	0.91
IGL01983:Stk10	APN	11	32589460	missense	probably benign	0.05
IGL03177:Stk10	APN	11	32614592	missense	probably damaging	1.00
IGL03183:Stk10	APN	11	32604143	missense	possibly damaging	0.50
coquet	UTSW	11	32577764	missense
legacy	UTSW	11	32604166	nonsense	probably null
mignon	UTSW	11	32587363	missense	probably damaging	1.00
R0481_stk10_383	UTSW	11	32614708	missense	probably damaging	1.00
FR4976:Stk10	UTSW	11	32614520	critical splice acceptor site	probably benign
R0003:Stk10	UTSW	11	32589460	missense	probably benign	0.05
R0008:Stk10	UTSW	11	32587305	splice site	probably benign
R0056:Stk10	UTSW	11	32617851	missense	possibly damaging	0.95
R0076:Stk10	UTSW	11	32603722	missense	probably benign
R0227:Stk10	UTSW	11	32617859	missense	probably damaging	1.00
R0440:Stk10	UTSW	11	32604190	missense	probably damaging	1.00
R0454:Stk10	UTSW	11	32596724	missense	probably damaging	0.99
R0481:Stk10	UTSW	11	32614708	missense	probably damaging	1.00
R0504:Stk10	UTSW	11	32617882	missense	probably benign	0.04
R0790:Stk10	UTSW	11	32598653	missense	probably benign	0.00
R1439:Stk10	UTSW	11	32617919	missense	probably damaging	0.98
R1539:Stk10	UTSW	11	32533440	missense	possibly damaging	0.85
R1770:Stk10	UTSW	11	32622464	missense	possibly damaging	0.94
R4304:Stk10	UTSW	11	32610634	missense	probably damaging	0.97
R4430:Stk10	UTSW	11	32533552	missense	possibly damaging	0.81
R4702:Stk10	UTSW	11	32555172	missense	probably benign	0.28
R4797:Stk10	UTSW	11	32598471	missense	probably benign	0.01
R5801:Stk10	UTSW	11	32596748	missense	probably benign	0.01
R5802:Stk10	UTSW	11	32596748	missense	probably benign	0.01
R6129:Stk10	UTSW	11	32615871	missense	probably damaging	1.00
R6154:Stk10	UTSW	11	32603654	splice site	probably null
R6175:Stk10	UTSW	11	32603761	missense	possibly damaging	0.46
R6185:Stk10	UTSW	11	32577749	missense	probably benign	0.13
R6520:Stk10	UTSW	11	32588839	missense	probably damaging	1.00
R6824:Stk10	UTSW	11	32587363	missense	probably damaging	1.00
R7259:Stk10	UTSW	11	32598497	missense	probably benign	0.00
R7649:Stk10	UTSW	11	32577764	missense
R8331:Stk10	UTSW	11	32588928	missense
R8847:Stk10	UTSW	11	32589427	missense
R9252:Stk10	UTSW	11	32588915	missense
R9367:Stk10	UTSW	11	32588878	missense
X0027:Stk10	UTSW	11	32587361	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AAATCCCCTGTGTGGAGTGC -3'
(R):5'- ATAGCTTTGTGGCTGGGCAC -3'

Sequencing Primer
(F):5'- ACAGAGAGGTTAAGTAACTTGCCCTG -3'
(R):5'- TCATTTGGAAAGCTGGACCC -3'

Posted On

2016-09-06