Mutagenetix > Incidental Mutation

Incidental Mutation 'R5447:Olfr175-ps1'

429155

Institutional Source

Beutler Lab

Gene Symbol

Olfr175-ps1

Ensembl Gene

ENSMUSG00000060663

Gene Name

olfactory receptor 175, pseudogene 1

Synonyms

GA_x54KRFPKG5P-55026345-55025418, GA_x54KRFPKG5P-54993816-54992890, MOR184-1, Olfr174, MOR184-10P

MMRRC Submission

043012-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R5447 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58823662-58826782 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 58824483 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 75 (C75*)

Ref Sequence

ENSEMBL: ENSMUSP00000150202 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079955] [ENSMUST00000215069]

AlphaFold

A0A140T8K4

Predicted Effect

probably null
Transcript: ENSMUST00000079955
AA Change: C75*

SMART Domains

Protein: ENSMUSP00000078873
Gene: ENSMUSG00000060663
AA Change: C75*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	1.3e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	305	6.8e-6	PFAM
Pfam:7tm_1	41	290	2.5e-18	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215069
AA Change: C75*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

97% (71/73)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5830473C10Rik	C	A	5: 90,584,310	A458E	probably damaging	Het
Abcb5	T	C	12: 118,927,326	I479V	probably damaging	Het
Adam30	A	G	3: 98,161,343	D164G	probably benign	Het
Adgrl3	T	A	5: 81,465,341		probably benign	Het
Adrb1	T	C	19: 56,723,087	I239T	probably benign	Het
B4galnt3	T	C	6: 120,215,057	T572A	probably benign	Het
Baz2b	T	C	2: 59,913,988	E1391G	probably damaging	Het
BC016579	A	G	16: 45,648,889	V72A	probably benign	Het
Btnl10	A	T	11: 58,922,318	I258F	probably benign	Het
Cdh5	A	T	8: 104,129,362	D309V	probably damaging	Het
Cdhr2	A	G	13: 54,733,250	D1042G	probably damaging	Het
Clk2	G	T	3: 89,167,191	V53F	possibly damaging	Het
Cyfip2	T	C	11: 46,291,586	D15G	possibly damaging	Het
Dip2b	C	T	15: 100,211,986	R1451C	probably damaging	Het
Dmbt1	A	G	7: 131,119,511	Y1836C	probably damaging	Het
Dysf	T	C	6: 84,195,263	F1905L	probably damaging	Het
E130114P18Rik	A	G	4: 97,690,718	S7P	unknown	Het
Fam110a	T	C	2: 151,970,709	E47G	probably damaging	Het
Gemin6	T	G	17: 80,227,749	V46G	probably damaging	Het
H2-Ke6	A	T	17: 34,026,912	V202D	probably damaging	Het
Helb	T	A	10: 120,102,901	D556V	possibly damaging	Het
Hoxd4	A	G	2: 74,727,343	E22G	probably damaging	Het
Il1rl2	T	G	1: 40,329,156	I162R	probably damaging	Het
Lhfpl5	A	G	17: 28,576,097	T33A	probably damaging	Het
Mapk8ip3	G	A	17: 24,899,189	A1283V	probably benign	Het
Mettl13	A	G	1: 162,535,880	V227A	probably benign	Het
Mmgt2	T	A	11: 62,664,998	C57*	probably null	Het
Muc4	G	C	16: 32,753,919	R1265P	probably benign	Het
Mylk2	T	C	2: 152,912,510	S175P	probably damaging	Het
Neu4	C	T	1: 94,022,418	T33M	probably damaging	Het
Nfs1	C	T	2: 156,142,136	R107H	probably benign	Het
Nfxl1	C	T	5: 72,529,169	R563Q	probably benign	Het
Nid1	A	G	13: 13,437,910	D70G	probably benign	Het
Nup160	C	A	2: 90,725,615	Q1220K	possibly damaging	Het
Olfr381	A	G	11: 73,486,176	V216A	probably benign	Het
Olfr51	G	A	11: 51,007,343	V124M	possibly damaging	Het
Olfr605	A	C	7: 103,442,940	M61R	probably damaging	Het
Pdgfrb	T	A	18: 61,068,108	V422E	probably damaging	Het
Pear1	G	A	3: 87,759,142	R85C	probably damaging	Het
Pkhd1	T	A	1: 20,239,385	M2780L	probably benign	Het
Ppp4r4	T	C	12: 103,584,151	V62A	possibly damaging	Het
Prol1	C	T	5: 88,328,266	P172S	unknown	Het
Proz	A	G	8: 13,072,578	I231V	probably benign	Het
Ptch1	T	G	13: 63,527,245	M718L	probably benign	Het
Ptprs	A	G	17: 56,429,128	C102R	possibly damaging	Het
Robo2	A	T	16: 73,973,766	Y490*	probably null	Het
Rptor	G	A	11: 119,843,713	G514D	probably damaging	Het
Scara5	CG	C	14: 65,759,662		probably null	Het
Skint6	T	A	4: 113,105,909	S442C	probably benign	Het
Snw1	T	C	12: 87,455,715	E303G	probably benign	Het
Sp110	C	G	1: 85,589,118	E219D	probably damaging	Het
Stam2	G	A	2: 52,736,293		probably benign	Het
Stk10	C	T	11: 32,604,166	Q618*	probably null	Het
Tmc3	A	T	7: 83,622,361	E907V	possibly damaging	Het
Ttn	A	T	2: 76,811,243	L5176Q	possibly damaging	Het
Ttn	T	A	2: 76,899,107		probably benign	Het
Vps39	T	C	2: 120,352,932	D19G	probably benign	Het
Zan	T	C	5: 137,472,191	S229G	probably damaging	Het
Zfp141	A	T	7: 42,475,559	C496*	probably null	Het
Zgrf1	T	C	3: 127,563,119	S665P	possibly damaging	Het

Other mutations in Olfr175-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01402:Olfr175-ps1	APN	16	58824595	missense	probably damaging	1.00
IGL01404:Olfr175-ps1	APN	16	58824595	missense	probably damaging	1.00
IGL01867:Olfr175-ps1	APN	16	58823974	missense	probably damaging	1.00
IGL02073:Olfr175-ps1	APN	16	58823806	missense	probably benign
IGL02393:Olfr175-ps1	APN	16	58824046	missense	probably damaging	0.99
IGL02474:Olfr175-ps1	APN	16	58824656	missense	probably benign
IGL02548:Olfr175-ps1	APN	16	58824328	missense	probably benign	0.42
IGL02948:Olfr175-ps1	APN	16	58824088	missense	probably benign	0.00
PIT4504001:Olfr175-ps1	UTSW	16	58824308	missense	probably benign	0.00
R0553:Olfr175-ps1	UTSW	16	58824155	missense	probably damaging	0.99
R1191:Olfr175-ps1	UTSW	16	58824559	missense	probably benign
R1201:Olfr175-ps1	UTSW	16	58823863	missense	probably damaging	0.98
R1458:Olfr175-ps1	UTSW	16	58824676	missense	probably null	0.68
R1469:Olfr175-ps1	UTSW	16	58824610	missense	probably benign
R1469:Olfr175-ps1	UTSW	16	58824610	missense	probably benign
R1843:Olfr175-ps1	UTSW	16	58824077	missense	probably damaging	0.99
R2147:Olfr175-ps1	UTSW	16	58824479	missense	probably damaging	0.99
R2421:Olfr175-ps1	UTSW	16	58824346	missense	probably damaging	1.00
R4370:Olfr175-ps1	UTSW	16	58824593	missense	probably benign	0.00
R4621:Olfr175-ps1	UTSW	16	58824106	missense	possibly damaging	0.94
R4622:Olfr175-ps1	UTSW	16	58824106	missense	possibly damaging	0.94
R4623:Olfr175-ps1	UTSW	16	58824106	missense	possibly damaging	0.94
R4711:Olfr175-ps1	UTSW	16	58824706	start codon destroyed	probably null	0.98
R5323:Olfr175-ps1	UTSW	16	58824703	missense	probably benign
R5457:Olfr175-ps1	UTSW	16	58824433	missense	probably damaging	0.97
R5546:Olfr175-ps1	UTSW	16	58824153	nonsense	probably null
R5623:Olfr175-ps1	UTSW	16	58824343	missense	probably benign	0.02
R5767:Olfr175-ps1	UTSW	16	58823953	missense	probably benign	0.42
R6270:Olfr175-ps1	UTSW	16	58824419	missense	probably damaging	1.00
R6449:Olfr175-ps1	UTSW	16	58824526	missense	probably damaging	1.00
R7356:Olfr175-ps1	UTSW	16	58824355	missense	probably damaging	0.99
R7521:Olfr175-ps1	UTSW	16	58823894	missense	probably benign	0.00
R7638:Olfr175-ps1	UTSW	16	58824595	missense	probably damaging	1.00
R7814:Olfr175-ps1	UTSW	16	58824002	missense	probably damaging	0.98
R9109:Olfr175-ps1	UTSW	16	58824314	missense	probably damaging	1.00
R9276:Olfr175-ps1	UTSW	16	58824371	missense	probably benign	0.09
R9681:Olfr175-ps1	UTSW	16	58823813	missense	possibly damaging	0.90
X0011:Olfr175-ps1	UTSW	16	58824663	missense	probably benign
Z1176:Olfr175-ps1	UTSW	16	58824307	frame shift	probably null
Z1177:Olfr175-ps1	UTSW	16	58824698	missense	possibly damaging	0.47

Predicted Primers

PCR Primer
(F):5'- CTCCTGTGGTCATTTGAAGGC -3'
(R):5'- ATAGGATTCTCAGACCACCCAG -3'

Sequencing Primer
(F):5'- AGGCAGAGCTTCTTGGACATC -3'
(R):5'- GATTCTCAGACCACCCAGACTTAAAG -3'

Posted On

2016-09-06