Incidental Mutation 'R5366:Ano1'
| ID |
429343 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Ano1
|
| Ensembl Gene |
ENSMUSG00000031075 |
| Gene Name |
anoctamin 1, calcium activated chloride channel |
| Synonyms |
Tmem16a |
| MMRRC Submission |
042944-MU
|
| Accession Numbers |
|
| Essential gene? |
Essential
(E-score: 1.000)
|
| Stock # |
R5366 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
7 |
| Chromosomal Location |
144588549-144751974 bp(-) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to A
at 144654209 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Serine
at position 113
(T113S)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000033393]
[ENSMUST00000118556]
[ENSMUST00000121758]
[ENSMUST00000131731]
[ENSMUST00000155175]
|
| AlphaFold |
no structure available at present |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000033393
AA Change: T248S
PolyPhen 2
Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
|
| SMART Domains |
Protein: ENSMUSP00000033393
Gene: ENSMUSG00000031075
AA Change: T248S
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
129 |
147 |
N/A |
INTRINSIC |
|
Pfam:Anoctamin
|
320 |
898 |
1.3e-149 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000118556
AA Change: T306S
PolyPhen 2
Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
|
| SMART Domains |
Protein: ENSMUSP00000113899
Gene: ENSMUSG00000031075
AA Change: T306S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Anoct_dimer
|
112 |
375 |
5.5e-83 |
PFAM |
|
Pfam:Anoctamin
|
378 |
955 |
6.7e-140 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000121758
AA Change: T305S
PolyPhen 2
Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
|
| SMART Domains |
Protein: ENSMUSP00000112616
Gene: ENSMUSG00000031075
AA Change: T305S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Anoct_dimer
|
54 |
317 |
7.1e-83 |
PFAM |
|
Pfam:Anoctamin
|
320 |
901 |
2.2e-139 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000131731
|
| SMART Domains |
Protein: ENSMUSP00000118206
Gene: ENSMUSG00000031075
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
129 |
147 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000141632
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000152531
AA Change: T113S
PolyPhen 2
Score 0.846 (Sensitivity: 0.83; Specificity: 0.93)
|
| SMART Domains |
Protein: ENSMUSP00000119653
Gene: ENSMUSG00000031075
AA Change: T113S
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:Anoct_dimer
|
2 |
205 |
4.4e-64 |
PFAM |
|
Pfam:Anoctamin
|
208 |
335 |
3e-36 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000155175
AA Change: T272S
PolyPhen 2
Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)
|
| SMART Domains |
Protein: ENSMUSP00000120306
Gene: ENSMUSG00000031075
AA Change: T272S
| Domain | Start | End | E-Value | Type |
|---|
|
low complexity region
|
153 |
171 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000208985
|
| Meta Mutation Damage Score |
0.1257 |
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.6%
- 10x: 97.2%
- 20x: 95.2%
|
| Validation Efficiency |
100% (77/77) |
| MGI Phenotype |
PHENOTYPE: Mice homozygous for a knockout allele exhibit postnatal death associated with aerophagia, slow postnatal weight gain, cyanosis, and abnormal tracheal morphology. Mice homozygous for a different knock-out allele exhibit proteinuria and intracellular endosomal vesicles in PTE cells. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 69 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcb5 |
T |
C |
12: 118,867,930 (GRCm38) |
N1229S |
possibly damaging |
Het |
| Adgre1 |
G |
A |
17: 57,402,817 (GRCm38) |
C158Y |
probably benign |
Het |
| Ahnak |
T |
A |
19: 9,016,735 (GRCm38) |
S5128T |
possibly damaging |
Het |
| Ankrd36 |
T |
A |
11: 5,592,841 (GRCm38) |
C322* |
probably null |
Het |
| Apeh |
A |
T |
9: 108,091,806 (GRCm38) |
S321T |
probably benign |
Het |
| Atg4d |
T |
A |
9: 21,268,652 (GRCm38) |
V273D |
probably damaging |
Het |
| Cacna2d4 |
A |
G |
6: 119,274,318 (GRCm38) |
D489G |
probably damaging |
Het |
| Cd47 |
T |
C |
16: 49,896,373 (GRCm38) |
F256L |
probably damaging |
Het |
| Cd84 |
A |
T |
1: 171,873,305 (GRCm38) |
D211V |
probably damaging |
Het |
| Cfap46 |
A |
G |
7: 139,650,886 (GRCm38) |
L942P |
probably damaging |
Het |
| Cfh |
A |
C |
1: 140,136,235 (GRCm38) |
C434W |
probably damaging |
Het |
| Chst2 |
T |
C |
9: 95,405,465 (GRCm38) |
D276G |
probably damaging |
Het |
| Ctsq |
T |
C |
13: 61,037,099 (GRCm38) |
T258A |
probably benign |
Het |
| Degs1 |
C |
A |
1: 182,279,362 (GRCm38) |
D111Y |
probably benign |
Het |
| Dhps |
T |
A |
8: 85,074,784 (GRCm38) |
D313E |
probably damaging |
Het |
| Dipk1c |
T |
A |
18: 84,730,595 (GRCm38) |
L106Q |
probably damaging |
Het |
| Dock9 |
A |
G |
14: 121,578,203 (GRCm38) |
C1645R |
probably damaging |
Het |
| Efcab7 |
A |
C |
4: 99,904,734 (GRCm38) |
D407A |
possibly damaging |
Het |
| Eif3a |
G |
T |
19: 60,779,533 (GRCm38) |
T189N |
probably benign |
Het |
| Ep300 |
T |
A |
15: 81,616,100 (GRCm38) |
L57M |
probably benign |
Het |
| Exoc6b |
T |
A |
6: 84,890,531 (GRCm38) |
I300L |
probably benign |
Het |
| Fzd8 |
T |
C |
18: 9,213,880 (GRCm38) |
S321P |
probably damaging |
Het |
| Garre1 |
A |
T |
7: 34,242,288 (GRCm38) |
M657K |
possibly damaging |
Het |
| Gm9920 |
T |
A |
15: 55,112,309 (GRCm38) |
|
probably benign |
Het |
| Gucy2c |
A |
G |
6: 136,720,741 (GRCm38) |
I668T |
probably damaging |
Het |
| Irgc |
C |
T |
7: 24,433,426 (GRCm38) |
|
probably benign |
Het |
| Jsrp1 |
G |
T |
10: 80,810,196 (GRCm38) |
S143* |
probably null |
Het |
| Kctd4 |
T |
C |
14: 75,962,819 (GRCm38) |
Y77H |
probably damaging |
Het |
| Klhdc1 |
T |
A |
12: 69,283,150 (GRCm38) |
I351N |
probably damaging |
Het |
| Klra17 |
C |
T |
6: 129,874,895 (GRCm38) |
E5K |
possibly damaging |
Het |
| Mapk8 |
A |
T |
14: 33,390,729 (GRCm38) |
V211E |
probably damaging |
Het |
| Mdn1 |
C |
T |
4: 32,723,690 (GRCm38) |
P2542L |
probably damaging |
Het |
| Mrc1 |
T |
C |
2: 14,321,914 (GRCm38) |
Y1208H |
probably benign |
Het |
| Mrps16 |
A |
T |
14: 20,391,455 (GRCm38) |
S94T |
probably benign |
Het |
| Muc5ac |
A |
G |
7: 141,807,550 (GRCm38) |
T1533A |
probably benign |
Het |
| Myh10 |
A |
T |
11: 68,760,692 (GRCm38) |
D287V |
probably damaging |
Het |
| Obscn |
T |
C |
11: 59,080,260 (GRCm38) |
T2476A |
probably damaging |
Het |
| Olfm2 |
T |
A |
9: 20,668,412 (GRCm38) |
T356S |
probably benign |
Het |
| Or4f60 |
T |
C |
2: 112,072,478 (GRCm38) |
Y35C |
possibly damaging |
Het |
| P2rx2 |
T |
A |
5: 110,341,828 (GRCm38) |
N108I |
probably damaging |
Het |
| Pbx4 |
A |
T |
8: 69,870,170 (GRCm38) |
T309S |
probably benign |
Het |
| Phyhip |
C |
A |
14: 70,466,855 (GRCm38) |
H171Q |
probably benign |
Het |
| Pou4f3 |
A |
G |
18: 42,395,754 (GRCm38) |
E254G |
probably damaging |
Het |
| Pxdn |
C |
A |
12: 30,002,900 (GRCm38) |
H845Q |
probably damaging |
Het |
| Rgcc |
T |
C |
14: 79,291,683 (GRCm38) |
T111A |
probably benign |
Het |
| Rptor |
G |
A |
11: 119,843,713 (GRCm38) |
G514D |
probably damaging |
Het |
| Scara5 |
CG |
C |
14: 65,759,662 (GRCm38) |
|
probably null |
Het |
| Sgsm1 |
C |
A |
5: 113,251,039 (GRCm38) |
E722D |
possibly damaging |
Het |
| Soat1 |
A |
T |
1: 156,444,611 (GRCm38) |
D101E |
probably benign |
Het |
| Spag5 |
T |
A |
11: 78,320,326 (GRCm38) |
|
probably null |
Het |
| Spata31 |
A |
T |
13: 64,920,459 (GRCm38) |
E140D |
probably damaging |
Het |
| Srrm2 |
T |
A |
17: 23,818,704 (GRCm38) |
S1537T |
probably benign |
Het |
| Stam2 |
G |
A |
2: 52,736,293 (GRCm38) |
|
probably benign |
Het |
| Tbc1d4 |
G |
T |
14: 101,607,976 (GRCm38) |
T162K |
possibly damaging |
Het |
| Tmem163 |
A |
C |
1: 127,500,305 (GRCm38) |
|
probably benign |
Het |
| Tmem43 |
T |
C |
6: 91,478,258 (GRCm38) |
V72A |
probably benign |
Het |
| Tmprss11c |
G |
A |
5: 86,282,134 (GRCm38) |
T24I |
possibly damaging |
Het |
| Trerf1 |
A |
T |
17: 47,315,190 (GRCm38) |
|
noncoding transcript |
Het |
| Trim44 |
A |
G |
2: 102,400,131 (GRCm38) |
L185P |
probably damaging |
Het |
| Tspyl1 |
A |
G |
10: 34,282,345 (GRCm38) |
D22G |
possibly damaging |
Het |
| Ttn |
A |
T |
2: 76,811,243 (GRCm38) |
L5176Q |
possibly damaging |
Het |
| Tyro3 |
G |
A |
2: 119,804,831 (GRCm38) |
R201Q |
probably damaging |
Het |
| Ubxn2a |
T |
A |
12: 4,880,741 (GRCm38) |
K206N |
probably benign |
Het |
| Usp17lb |
G |
T |
7: 104,840,408 (GRCm38) |
H436Q |
possibly damaging |
Het |
| Vps9d1 |
A |
G |
8: 123,245,114 (GRCm38) |
I584T |
possibly damaging |
Het |
| Vrk1 |
T |
A |
12: 106,055,819 (GRCm38) |
M131K |
possibly damaging |
Het |
| Wdr87-ps |
A |
C |
7: 29,533,539 (GRCm38) |
|
noncoding transcript |
Het |
| Zfp827 |
A |
T |
8: 79,185,704 (GRCm38) |
K986N |
possibly damaging |
Het |
| Zswim4 |
A |
G |
8: 84,212,790 (GRCm38) |
M821T |
probably benign |
Het |
|
| Other mutations in Ano1 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00595:Ano1
|
APN |
7 |
144,638,513 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL00754:Ano1
|
APN |
7 |
144,597,231 (GRCm38) |
missense |
probably damaging |
0.98 |
|
IGL00780:Ano1
|
APN |
7 |
144,655,630 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL00918:Ano1
|
APN |
7 |
144,644,752 (GRCm38) |
splice site |
probably benign |
|
|
IGL01112:Ano1
|
APN |
7 |
144,637,145 (GRCm38) |
missense |
possibly damaging |
0.52 |
|
IGL01285:Ano1
|
APN |
7 |
144,644,742 (GRCm38) |
missense |
probably benign |
0.00 |
|
IGL01285:Ano1
|
APN |
7 |
144,595,538 (GRCm38) |
missense |
probably damaging |
0.98 |
|
IGL01308:Ano1
|
APN |
7 |
144,595,498 (GRCm38) |
missense |
probably damaging |
0.99 |
|
IGL01407:Ano1
|
APN |
7 |
144,637,111 (GRCm38) |
missense |
probably benign |
0.22 |
|
IGL01672:Ano1
|
APN |
7 |
144,655,675 (GRCm38) |
missense |
probably damaging |
0.96 |
|
IGL01920:Ano1
|
APN |
7 |
144,611,454 (GRCm38) |
splice site |
probably benign |
|
|
IGL01926:Ano1
|
APN |
7 |
144,610,875 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
IGL02164:Ano1
|
APN |
7 |
144,637,181 (GRCm38) |
missense |
possibly damaging |
0.91 |
|
IGL02190:Ano1
|
APN |
7 |
144,618,883 (GRCm38) |
missense |
probably benign |
0.41 |
|
IGL02214:Ano1
|
APN |
7 |
144,655,708 (GRCm38) |
missense |
possibly damaging |
0.80 |
|
IGL02299:Ano1
|
APN |
7 |
144,590,075 (GRCm38) |
missense |
possibly damaging |
0.80 |
|
IGL02567:Ano1
|
APN |
7 |
144,611,625 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03131:Ano1
|
APN |
7 |
144,603,585 (GRCm38) |
missense |
possibly damaging |
0.90 |
|
IGL03291:Ano1
|
APN |
7 |
144,621,675 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03299:Ano1
|
APN |
7 |
144,654,256 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL03394:Ano1
|
APN |
7 |
144,595,439 (GRCm38) |
splice site |
probably null |
|
|
PIT4434001:Ano1
|
UTSW |
7 |
144,610,895 (GRCm38) |
missense |
probably benign |
0.28 |
|
R0502:Ano1
|
UTSW |
7 |
144,597,215 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0595:Ano1
|
UTSW |
7 |
144,590,153 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R0732:Ano1
|
UTSW |
7 |
144,619,488 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R0970:Ano1
|
UTSW |
7 |
144,595,571 (GRCm38) |
missense |
probably benign |
0.02 |
|
R0988:Ano1
|
UTSW |
7 |
144,633,653 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R1074:Ano1
|
UTSW |
7 |
144,611,680 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1301:Ano1
|
UTSW |
7 |
144,633,689 (GRCm38) |
missense |
possibly damaging |
0.60 |
|
R1528:Ano1
|
UTSW |
7 |
144,595,566 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2018:Ano1
|
UTSW |
7 |
144,654,250 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2056:Ano1
|
UTSW |
7 |
144,648,052 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2057:Ano1
|
UTSW |
7 |
144,648,052 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2058:Ano1
|
UTSW |
7 |
144,648,052 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2059:Ano1
|
UTSW |
7 |
144,611,390 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2860:Ano1
|
UTSW |
7 |
144,590,012 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R2861:Ano1
|
UTSW |
7 |
144,590,012 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3770:Ano1
|
UTSW |
7 |
144,595,569 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3970:Ano1
|
UTSW |
7 |
144,607,963 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4179:Ano1
|
UTSW |
7 |
144,650,505 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4489:Ano1
|
UTSW |
7 |
144,611,742 (GRCm38) |
missense |
probably benign |
0.00 |
|
R4678:Ano1
|
UTSW |
7 |
144,669,552 (GRCm38) |
missense |
probably benign |
0.01 |
|
R4915:Ano1
|
UTSW |
7 |
144,611,375 (GRCm38) |
missense |
possibly damaging |
0.69 |
|
R5114:Ano1
|
UTSW |
7 |
144,657,083 (GRCm38) |
missense |
possibly damaging |
0.71 |
|
R5362:Ano1
|
UTSW |
7 |
144,648,600 (GRCm38) |
unclassified |
probably benign |
|
|
R5364:Ano1
|
UTSW |
7 |
144,637,204 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5387:Ano1
|
UTSW |
7 |
144,648,619 (GRCm38) |
missense |
probably benign |
|
|
R5762:Ano1
|
UTSW |
7 |
144,648,037 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R5857:Ano1
|
UTSW |
7 |
144,637,103 (GRCm38) |
missense |
probably benign |
0.02 |
|
R6091:Ano1
|
UTSW |
7 |
144,669,434 (GRCm38) |
missense |
probably benign |
0.12 |
|
R6093:Ano1
|
UTSW |
7 |
144,611,377 (GRCm38) |
missense |
possibly damaging |
0.72 |
|
R6177:Ano1
|
UTSW |
7 |
144,678,741 (GRCm38) |
missense |
possibly damaging |
0.79 |
|
R6246:Ano1
|
UTSW |
7 |
144,633,725 (GRCm38) |
missense |
possibly damaging |
0.82 |
|
R6274:Ano1
|
UTSW |
7 |
144,618,863 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6323:Ano1
|
UTSW |
7 |
144,611,686 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
R6574:Ano1
|
UTSW |
7 |
144,607,916 (GRCm38) |
critical splice donor site |
probably null |
|
|
R6782:Ano1
|
UTSW |
7 |
144,621,687 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6880:Ano1
|
UTSW |
7 |
144,644,742 (GRCm38) |
missense |
probably benign |
0.00 |
|
R6909:Ano1
|
UTSW |
7 |
144,655,731 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R7066:Ano1
|
UTSW |
7 |
144,637,086 (GRCm38) |
missense |
probably benign |
0.35 |
|
R7073:Ano1
|
UTSW |
7 |
144,638,552 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R7146:Ano1
|
UTSW |
7 |
144,655,656 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7420:Ano1
|
UTSW |
7 |
144,655,641 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7874:Ano1
|
UTSW |
7 |
144,621,724 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8468:Ano1
|
UTSW |
7 |
144,655,620 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8867:Ano1
|
UTSW |
7 |
144,669,660 (GRCm38) |
missense |
possibly damaging |
0.66 |
|
R8923:Ano1
|
UTSW |
7 |
144,650,551 (GRCm38) |
missense |
possibly damaging |
0.61 |
|
R9215:Ano1
|
UTSW |
7 |
144,595,605 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9281:Ano1
|
UTSW |
7 |
144,595,581 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9572:Ano1
|
UTSW |
7 |
144,650,556 (GRCm38) |
critical splice acceptor site |
probably null |
|
|
R9668:Ano1
|
UTSW |
7 |
144,610,842 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9681:Ano1
|
UTSW |
7 |
144,590,156 (GRCm38) |
missense |
possibly damaging |
0.68 |
|
R9756:Ano1
|
UTSW |
7 |
144,608,929 (GRCm38) |
missense |
probably benign |
0.45 |
|
R9780:Ano1
|
UTSW |
7 |
144,655,621 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9792:Ano1
|
UTSW |
7 |
144,621,697 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9793:Ano1
|
UTSW |
7 |
144,621,697 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R9795:Ano1
|
UTSW |
7 |
144,621,697 (GRCm38) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TCCTCCTCCAAACTGAGCAG -3'
(R):5'- TCTGCTAACCTCATGCCCAG -3'
Sequencing Primer
(F):5'- TCCAAACTGAGCAGCCAGGG -3'
(R):5'- TAACCTCATGCCCAGGGACAG -3'
|
| Posted On |
2016-09-06 |
Incidental Mutation