Mutagenetix > Incidental Mutation

Incidental Mutation 'R5368:Wdfy3'

429472

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

2610509D04Rik, Ggtb3, Bchs, D5Ertd66e, Bwf1, Alfy

MMRRC Submission

043204-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.943) question?

Stock #

R5368 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

101980822-102217787 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 102020724 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Methionine at position 2527 (L2527M)

Ref Sequence

ENSEMBL: ENSMUSP00000134244 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000053177
AA Change: L2509M

PolyPhen 2 Score 0.906 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: L2509M

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000174598
AA Change: L2527M

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: L2527M

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000212024
AA Change: L2513M

PolyPhen 2 Score 0.891 (Sensitivity: 0.82; Specificity: 0.94)

Meta Mutation Damage Score

0.0796

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

97% (61/63)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933402N03Rik	T	A	7: 130,740,925 (GRCm39)	H97L	possibly damaging	Het
Abca9	T	A	11: 110,036,372 (GRCm39)	N579I	probably damaging	Het
Acbd3	A	T	1: 180,549,660 (GRCm39)		probably benign	Het
Ankrd50	A	T	3: 38,509,199 (GRCm39)	I1056N	probably damaging	Het
Ceacam18	T	C	7: 43,291,458 (GRCm39)	V300A	probably benign	Het
Cfap46	C	A	7: 139,207,389 (GRCm39)	R1843S	possibly damaging	Het
D430041D05Rik	A	G	2: 104,078,629 (GRCm39)	V1229A	probably damaging	Het
Dbx2	G	A	15: 95,538,522 (GRCm39)	S206L	probably benign	Het
Dnah11	A	T	12: 117,918,628 (GRCm39)	D1530E	probably damaging	Het
Ecm2	A	C	13: 49,674,419 (GRCm39)	T280P	probably benign	Het
Emc2	G	A	15: 43,375,207 (GRCm39)		probably null	Het
Epb42	C	T	2: 120,849,943 (GRCm39)	V689I	probably benign	Het
Fam114a1	T	A	5: 65,163,452 (GRCm39)	M209K	possibly damaging	Het
Fam117a	C	T	11: 95,266,459 (GRCm39)	S193F	probably damaging	Het
Foxp2	A	T	6: 15,377,913 (GRCm39)		probably benign	Het
Frmd6	T	A	12: 70,910,874 (GRCm39)	C19*	probably null	Het
Fyb1	A	G	15: 6,610,159 (GRCm39)		probably null	Het
Gata6	C	A	18: 11,063,059 (GRCm39)	H442Q	possibly damaging	Het
Gldc	A	G	19: 30,135,921 (GRCm39)	S160P	probably benign	Het
Gm3952	A	G	8: 129,472,455 (GRCm39)	S1626P	possibly damaging	Het
Gm9762	T	A	3: 78,873,742 (GRCm39)		noncoding transcript	Het
Gtpbp2	T	C	17: 46,477,230 (GRCm39)		probably benign	Het
Hgd	A	T	16: 37,410,113 (GRCm39)	T50S	probably benign	Het
Itpr1	C	T	6: 108,364,459 (GRCm39)	T22M	probably damaging	Het
Kif26b	G	A	1: 178,743,449 (GRCm39)	E1182K	probably damaging	Het
Kirrel3	A	G	9: 34,919,034 (GRCm39)	E230G	probably damaging	Het
Leng8	A	G	7: 4,142,987 (GRCm39)	Y88C	probably damaging	Het
Lpo	T	C	11: 87,711,895 (GRCm39)	D54G	possibly damaging	Het
Lypd2	G	T	15: 74,604,908 (GRCm39)	A29E	probably benign	Het
Mdn1	C	T	4: 32,723,690 (GRCm39)	P2542L	probably damaging	Het
Mical3	T	C	6: 120,936,434 (GRCm39)	Y1364C	probably damaging	Het
Mroh2b	A	G	15: 4,935,054 (GRCm39)	N163S	probably damaging	Het
Mtf1	G	A	4: 124,718,872 (GRCm39)	C295Y	probably damaging	Het
Nbn	T	A	4: 15,969,391 (GRCm39)	L212Q	probably damaging	Het
Obscn	A	T	11: 58,959,852 (GRCm39)		probably null	Het
Or10ag56	T	A	2: 87,139,126 (GRCm39)		probably null	Het
Or4c110	A	G	2: 88,832,435 (GRCm39)	S66P	probably damaging	Het
Peli1	A	G	11: 21,098,389 (GRCm39)	T375A	probably damaging	Het
Picalm	T	C	7: 89,856,803 (GRCm39)	*611Q	probably null	Het
Plch1	G	T	3: 63,609,394 (GRCm39)	Q938K	possibly damaging	Het
Plxnb2	A	G	15: 89,043,796 (GRCm39)	V1352A	possibly damaging	Het
Pmepa1	G	A	2: 173,070,115 (GRCm39)	R147W	probably damaging	Het
Prdm16	T	G	4: 154,429,848 (GRCm39)	K373Q	probably damaging	Het
Qki	T	G	17: 10,457,964 (GRCm39)	E135A	probably damaging	Het
Rad50	T	C	11: 53,575,073 (GRCm39)	K556E	probably benign	Het
Scara5	CG	C	14: 65,997,111 (GRCm39)		probably null	Het
Sin3a	T	A	9: 57,018,084 (GRCm39)	D834E	possibly damaging	Het
Smg8	G	A	11: 86,971,086 (GRCm39)	S895L	probably benign	Het
Sorl1	T	C	9: 41,890,686 (GRCm39)	I1944M	probably benign	Het
Stam2	G	A	2: 52,626,305 (GRCm39)		probably benign	Het
Tmprss7	A	T	16: 45,481,252 (GRCm39)	W645R	probably damaging	Het
Tns1	T	C	1: 73,980,176 (GRCm39)	M1111V	probably benign	Het
Ttn	A	C	2: 76,608,726 (GRCm39)	D17763E	probably damaging	Het
Ttn	A	T	2: 76,641,587 (GRCm39)	L5176Q	possibly damaging	Het
Ubr4	G	A	4: 139,124,839 (GRCm39)		probably benign	Het
Usp31	T	C	7: 121,260,588 (GRCm39)	H637R	probably damaging	Het
Vps50	A	C	6: 3,567,739 (GRCm39)	E545A	possibly damaging	Het
Wfdc2	T	C	2: 164,405,354 (GRCm39)	V85A	possibly damaging	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	102,063,204 (GRCm39)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	102,059,896 (GRCm39)	splice site	probably benign
IGL01288:Wdfy3	APN	5	102,049,857 (GRCm39)	splice site	probably null
IGL01323:Wdfy3	APN	5	102,042,930 (GRCm39)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	102,091,986 (GRCm39)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	102,047,897 (GRCm39)	missense	probably benign
IGL01560:Wdfy3	APN	5	102,105,352 (GRCm39)	nonsense	probably null
IGL01566:Wdfy3	APN	5	102,044,454 (GRCm39)	splice site	probably benign
IGL01616:Wdfy3	APN	5	102,061,126 (GRCm39)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	102,055,354 (GRCm39)	missense	probably benign
IGL01791:Wdfy3	APN	5	102,085,278 (GRCm39)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	102,071,947 (GRCm39)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	102,042,894 (GRCm39)	nonsense	probably null
IGL02121:Wdfy3	APN	5	102,046,376 (GRCm39)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	102,109,023 (GRCm39)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	102,070,475 (GRCm39)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	102,036,058 (GRCm39)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	102,044,341 (GRCm39)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	102,055,453 (GRCm39)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	102,116,786 (GRCm39)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	102,003,337 (GRCm39)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	102,042,778 (GRCm39)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	102,083,863 (GRCm39)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	102,014,142 (GRCm39)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,992,778 (GRCm39)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,992,465 (GRCm39)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	102,048,016 (GRCm39)	missense	probably damaging	1.00
Esurient	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	102,077,847 (GRCm39)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	102,030,827 (GRCm39)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,996,215 (GRCm39)	missense	probably damaging	1.00
R0025:Wdfy3	UTSW	5	101,992,912 (GRCm39)	missense	probably damaging	0.98
R0031:Wdfy3	UTSW	5	102,037,161 (GRCm39)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	102,091,899 (GRCm39)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,992,480 (GRCm39)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	102,035,971 (GRCm39)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	102,065,277 (GRCm39)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	102,015,958 (GRCm39)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	102,096,832 (GRCm39)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	102,105,309 (GRCm39)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	102,038,655 (GRCm39)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	102,054,051 (GRCm39)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,984,038 (GRCm39)	missense	probably benign
R0787:Wdfy3	UTSW	5	102,105,254 (GRCm39)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	102,017,917 (GRCm39)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	102,030,832 (GRCm39)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	102,023,797 (GRCm39)	missense	probably benign
R1350:Wdfy3	UTSW	5	102,046,418 (GRCm39)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	102,032,080 (GRCm39)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,999,176 (GRCm39)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	102,085,604 (GRCm39)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	102,065,445 (GRCm39)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,991,947 (GRCm39)	missense	probably benign
R1633:Wdfy3	UTSW	5	102,129,414 (GRCm39)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	102,023,781 (GRCm39)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	102,089,313 (GRCm39)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	102,074,391 (GRCm39)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,991,931 (GRCm39)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	102,096,795 (GRCm39)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	102,042,865 (GRCm39)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	102,063,242 (GRCm39)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	102,036,052 (GRCm39)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	102,065,301 (GRCm39)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	102,067,275 (GRCm39)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	102,099,178 (GRCm39)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	102,008,352 (GRCm39)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	102,042,926 (GRCm39)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	102,046,291 (GRCm39)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	102,055,408 (GRCm39)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	102,037,150 (GRCm39)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	102,036,189 (GRCm39)	splice site	probably benign
R2406:Wdfy3	UTSW	5	102,036,125 (GRCm39)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	102,023,796 (GRCm39)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	102,091,988 (GRCm39)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	102,009,266 (GRCm39)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	102,085,466 (GRCm39)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	102,092,105 (GRCm39)	nonsense	probably null
R3947:Wdfy3	UTSW	5	102,017,902 (GRCm39)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	102,071,961 (GRCm39)	splice site	probably benign
R4065:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	102,070,313 (GRCm39)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	102,047,924 (GRCm39)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	102,070,500 (GRCm39)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	102,058,850 (GRCm39)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	102,054,011 (GRCm39)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	102,031,949 (GRCm39)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	102,091,800 (GRCm39)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	102,077,894 (GRCm39)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	102,091,809 (GRCm39)	missense	probably damaging	1.00
R4869:Wdfy3	UTSW	5	102,042,787 (GRCm39)	missense	probably damaging	0.98
R4971:Wdfy3	UTSW	5	102,096,838 (GRCm39)	nonsense	probably null
R4973:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	102,090,985 (GRCm39)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	102,042,803 (GRCm39)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	102,015,972 (GRCm39)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	102,091,969 (GRCm39)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,997,133 (GRCm39)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,994,972 (GRCm39)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	102,100,849 (GRCm39)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	102,067,312 (GRCm39)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	102,044,425 (GRCm39)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,984,140 (GRCm39)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	102,009,314 (GRCm39)	missense	possibly damaging	0.69
R5877:Wdfy3	UTSW	5	102,017,855 (GRCm39)	missense	probably damaging	1.00
R5988:Wdfy3	UTSW	5	102,032,004 (GRCm39)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,999,225 (GRCm39)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,997,289 (GRCm39)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	102,046,295 (GRCm39)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	102,020,831 (GRCm39)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	102,116,812 (GRCm39)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	102,061,045 (GRCm39)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	102,101,032 (GRCm39)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	102,031,911 (GRCm39)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	102,065,297 (GRCm39)	nonsense	probably null
R6809:Wdfy3	UTSW	5	102,071,813 (GRCm39)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	102,100,865 (GRCm39)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,991,932 (GRCm39)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	102,042,775 (GRCm39)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	102,055,384 (GRCm39)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	102,003,415 (GRCm39)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	102,063,303 (GRCm39)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	102,091,758 (GRCm39)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	102,049,785 (GRCm39)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,984,074 (GRCm39)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	102,003,389 (GRCm39)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	102,105,366 (GRCm39)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	102,030,354 (GRCm39)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	102,083,925 (GRCm39)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	102,003,252 (GRCm39)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,996,223 (GRCm39)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	102,099,265 (GRCm39)	nonsense	probably null
R7810:Wdfy3	UTSW	5	102,042,940 (GRCm39)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	102,089,476 (GRCm39)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	102,085,287 (GRCm39)	missense	probably benign
R8507:Wdfy3	UTSW	5	102,020,767 (GRCm39)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,999,219 (GRCm39)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	102,033,064 (GRCm39)	missense	probably benign
R8710:Wdfy3	UTSW	5	102,030,349 (GRCm39)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	102,077,951 (GRCm39)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	102,030,446 (GRCm39)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	102,065,421 (GRCm39)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,993,231 (GRCm39)	intron	probably benign
R8968:Wdfy3	UTSW	5	102,011,983 (GRCm39)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	102,096,764 (GRCm39)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,993,058 (GRCm39)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,995,040 (GRCm39)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	102,000,451 (GRCm39)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	102,030,512 (GRCm39)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	102,091,831 (GRCm39)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	102,078,830 (GRCm39)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,996,359 (GRCm39)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	102,089,358 (GRCm39)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	102,000,478 (GRCm39)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	102,078,716 (GRCm39)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	102,055,333 (GRCm39)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	102,100,957 (GRCm39)	missense
R9548:Wdfy3	UTSW	5	102,033,059 (GRCm39)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	102,047,899 (GRCm39)	missense	probably benign
R9750:Wdfy3	UTSW	5	102,077,960 (GRCm39)	missense	probably benign	0.00
R9766:Wdfy3	UTSW	5	102,042,866 (GRCm39)	missense	possibly damaging	0.90
R9771:Wdfy3	UTSW	5	102,000,195 (GRCm39)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	102,048,107 (GRCm39)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- TGGTCTAACAACAGACACAGAG -3'
(R):5'- TAAACTGTCCCCACATGGCC -3'

Sequencing Primer
(F):5'- GAATTAGGACTCAGTTGTGAACCCC -3'
(R):5'- ACATGGCCCCACCCCAC -3'

Posted On

2016-09-06