|Institutional Source||Beutler Lab|
|Gene Name||dual specificity phosphatase 4|
|Synonyms||MKP2, E130306H24Rik, 2700078F24Rik|
|Is this an essential gene?||Probably non essential (E-score: 0.179)|
|Stock #||R4826 (G1)|
|Chromosomal Location||34807297-34819894 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 34818517 bp|
|Amino Acid Change||Phenylalanine to Isoleucine at position 311 (F311I)|
|Ref Sequence||ENSEMBL: ENSMUSP00000033930 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033930]|
|Predicted Effect||probably damaging
AA Change: F311I
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: F311I
|Meta Mutation Damage Score||0.7760|
|Coding Region Coverage||
|Validation Efficiency||100% (72/72)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit a decrease in B cell apoptosis of bone marrow-derived, IL-7-dependent pro-B lymphocytes. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dusp4||
(F):5'- TAGTGGCGAATCCCTGAAGC -3'
(R):5'- CAGAGACGAGTCCTAACAGC -3'
(F):5'- TGAAGCCCGCCAAGGTC -3'
(R):5'- ACGAGTCCTAACAGCTGGGG -3'