Mutagenetix > Incidental Mutation

Incidental Mutation 'R5509:Or8g21'

431078

Institutional Source

Beutler Lab

Gene Symbol

Or8g21

Ensembl Gene

ENSMUSG00000059595

Gene Name

olfactory receptor family 8 subfamily G member 21

Synonyms

MOR171-11, GA_x6K02T2PVTD-32691280-32690354, Olfr935

MMRRC Submission

043070-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R5509 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38905803-38906729 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38905924 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 269 (D269G)

Ref Sequence

ENSEMBL: ENSMUSP00000149193 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080748] [ENSMUST00000214157]

AlphaFold

Q8VG16

Predicted Effect

probably benign
Transcript: ENSMUST00000080748
AA Change: D269G

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000079572
Gene: ENSMUSG00000059595
AA Change: D269G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4e-53	PFAM
Pfam:7tm_1	41	290	1.2e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214157
AA Change: D269G

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 98.6%
3x: 97.4%
10x: 95.5%
20x: 91.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc10	G	C	17: 46,635,185 (GRCm39)	Q273E	probably benign	Het
Acly	C	T	11: 100,405,805 (GRCm39)	R329Q	probably damaging	Het
Acsm2	T	C	7: 119,172,840 (GRCm39)	S152P	probably damaging	Het
Adamtsl4	A	T	3: 95,588,667 (GRCm39)	I515N	probably benign	Het
Ank3	G	A	10: 69,838,395 (GRCm39)	R1566K	possibly damaging	Het
Atp9a	T	C	2: 168,481,857 (GRCm39)	D879G	probably damaging	Het
Bmp8b	G	A	4: 123,008,369 (GRCm39)	D112N	possibly damaging	Het
Camk2d	T	C	3: 126,633,965 (GRCm39)	W496R	probably damaging	Het
Ccdc192	G	A	18: 57,671,156 (GRCm39)		probably null	Het
Cflar	T	C	1: 58,791,551 (GRCm39)	F285L	probably benign	Het
Cox16	T	C	12: 81,519,032 (GRCm39)	T176A	probably benign	Het
Cux1	G	T	5: 136,304,171 (GRCm39)	A1214D	probably benign	Het
Cyb5d1	C	A	11: 69,284,561 (GRCm39)		probably null	Het
Dennd6b	G	A	15: 89,069,225 (GRCm39)	P575S	probably damaging	Het
Fbxl2	A	T	9: 113,814,415 (GRCm39)		probably null	Het
Gfpt2	T	C	11: 49,717,973 (GRCm39)	F483L	possibly damaging	Het
Glrx3	T	C	7: 137,046,751 (GRCm39)	C48R	probably damaging	Het
Invs	G	A	4: 48,396,337 (GRCm39)	V281M	probably damaging	Het
Kif13a	G	A	13: 46,905,591 (GRCm39)	A784V	probably benign	Het
Kmt2d	T	C	15: 98,737,557 (GRCm39)		probably benign	Het
Lmod2	A	G	6: 24,603,888 (GRCm39)	T288A	probably damaging	Het
Lrrc37a	T	C	11: 103,391,361 (GRCm39)	K1355E	probably benign	Het
Magi3	A	T	3: 103,922,818 (GRCm39)	S1300T	probably benign	Het
Mdm2	A	C	10: 117,526,517 (GRCm39)	D307E	probably damaging	Het
Mllt6	G	A	11: 97,560,326 (GRCm39)	S210N	possibly damaging	Het
Mroh4	T	C	15: 74,478,003 (GRCm39)	Y901C	probably benign	Het
Mtmr14	G	T	6: 113,230,768 (GRCm39)		probably null	Het
Mug2	A	T	6: 122,061,340 (GRCm39)	Q1420L	possibly damaging	Het
Net1	T	C	13: 3,934,320 (GRCm39)	Q459R	probably benign	Het
Nle1	T	C	11: 82,794,008 (GRCm39)	R343G	possibly damaging	Het
Npat	C	A	9: 53,481,542 (GRCm39)	N1083K	probably benign	Het
Nrp1	G	A	8: 129,152,396 (GRCm39)	G202R	possibly damaging	Het
Nsun4	C	T	4: 115,908,974 (GRCm39)	V529I	possibly damaging	Het
Or4b1d	T	A	2: 89,969,236 (GRCm39)	L82F	probably damaging	Het
Or4z4	T	A	19: 12,076,341 (GRCm39)	I221F	possibly damaging	Het
Or5b117	A	T	19: 13,431,332 (GRCm39)	L183Q	probably damaging	Het
Podxl	A	T	6: 31,503,548 (GRCm39)	N264K	probably benign	Het
Ptprm	C	T	17: 66,996,353 (GRCm39)	A1245T	probably damaging	Het
Rdh12	T	A	12: 79,257,558 (GRCm39)		probably null	Het
Ryr2	T	A	13: 11,760,487 (GRCm39)	Y1532F	probably damaging	Het
Senp2	T	C	16: 21,859,272 (GRCm39)	F441S	probably damaging	Het
Snap23	C	A	2: 120,425,346 (GRCm39)	P111T	probably benign	Het
Sox2	A	G	3: 34,704,938 (GRCm39)	D125G	probably damaging	Het
Syne2	T	C	12: 75,968,018 (GRCm39)	W923R	probably damaging	Het
Tbc1d2b	T	C	9: 90,101,022 (GRCm39)	E656G	probably damaging	Het
Tbcd	A	G	11: 121,492,838 (GRCm39)	T892A	probably benign	Het
Tg	A	G	15: 66,699,142 (GRCm39)	I24V	probably benign	Het
Trpm3	A	T	19: 22,964,622 (GRCm39)	K1372N	probably damaging	Het
Ubc	A	T	5: 125,464,339 (GRCm39)	N329K	probably benign	Het
Vmn2r106	T	A	17: 20,498,684 (GRCm39)	H409L	probably damaging	Het
Wdfy3	T	A	5: 102,009,314 (GRCm39)	N2751Y	possibly damaging	Het
Zfp518a	T	A	19: 40,903,845 (GRCm39)	I1258K	possibly damaging	Het
Zfp91	T	C	19: 12,756,451 (GRCm39)	E131G	probably damaging	Het
Zfyve26	T	A	12: 79,293,295 (GRCm39)	R2027W	probably damaging	Het

Other mutations in Or8g21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01522:Or8g21	APN	9	38,906,396 (GRCm39)	missense	probably benign	0.08
IGL01671:Or8g21	APN	9	38,906,149 (GRCm39)	missense	probably benign	0.05
IGL02331:Or8g21	APN	9	38,906,402 (GRCm39)	missense	probably damaging	0.99
IGL03069:Or8g21	APN	9	38,906,728 (GRCm39)	start codon destroyed	probably null	0.98
IGL03143:Or8g21	APN	9	38,906,732 (GRCm39)	utr 5 prime	probably benign
R0149:Or8g21	UTSW	9	38,905,880 (GRCm39)	missense	probably benign	0.05
R0400:Or8g21	UTSW	9	38,906,494 (GRCm39)	missense	probably damaging	1.00
R1818:Or8g21	UTSW	9	38,905,902 (GRCm39)	missense	possibly damaging	0.61
R2092:Or8g21	UTSW	9	38,906,485 (GRCm39)	missense	probably damaging	1.00
R2151:Or8g21	UTSW	9	38,906,012 (GRCm39)	missense	probably damaging	1.00
R2166:Or8g21	UTSW	9	38,906,513 (GRCm39)	nonsense	probably null
R2347:Or8g21	UTSW	9	38,905,805 (GRCm39)	makesense	probably null
R4404:Or8g21	UTSW	9	38,905,865 (GRCm39)	missense	possibly damaging	0.77
R4406:Or8g21	UTSW	9	38,905,865 (GRCm39)	missense	possibly damaging	0.77
R5152:Or8g21	UTSW	9	38,906,473 (GRCm39)	missense	possibly damaging	0.88
R5467:Or8g21	UTSW	9	38,906,200 (GRCm39)	missense	probably benign	0.14
R5954:Or8g21	UTSW	9	38,906,711 (GRCm39)	missense	probably damaging	1.00
R6647:Or8g21	UTSW	9	38,906,210 (GRCm39)	missense	possibly damaging	0.55
R6680:Or8g21	UTSW	9	38,905,954 (GRCm39)	missense	probably damaging	1.00
R6928:Or8g21	UTSW	9	38,905,928 (GRCm39)	missense	probably benign	0.03
R7242:Or8g21	UTSW	9	38,906,437 (GRCm39)	missense	probably benign	0.31
R7271:Or8g21	UTSW	9	38,905,953 (GRCm39)	nonsense	probably null
R7309:Or8g21	UTSW	9	38,906,576 (GRCm39)	missense	probably damaging	1.00
R7775:Or8g21	UTSW	9	38,906,203 (GRCm39)	missense	probably damaging	1.00
R7778:Or8g21	UTSW	9	38,906,203 (GRCm39)	missense	probably damaging	1.00
R7866:Or8g21	UTSW	9	38,906,027 (GRCm39)	missense	not run
R8280:Or8g21	UTSW	9	38,906,075 (GRCm39)	missense	probably benign
R8334:Or8g21	UTSW	9	38,905,889 (GRCm39)	missense	probably benign	0.00
R8841:Or8g21	UTSW	9	38,905,879 (GRCm39)	missense	possibly damaging	0.95
R9161:Or8g21	UTSW	9	38,905,816 (GRCm39)	missense	possibly damaging	0.70
R9169:Or8g21	UTSW	9	38,906,573 (GRCm39)	missense	probably benign	0.23
R9659:Or8g21	UTSW	9	38,906,296 (GRCm39)	missense	possibly damaging	0.64
R9739:Or8g21	UTSW	9	38,906,302 (GRCm39)	nonsense	probably null
R9788:Or8g21	UTSW	9	38,906,296 (GRCm39)	missense	possibly damaging	0.64

Predicted Primers

PCR Primer
(F):5'- CCCTGGAATTGAAGACACAAGGTG -3'
(R):5'- ACGCTGACCATCCTCAGTTC -3'

Sequencing Primer
(F):5'- TTGAAGACACAAGGTGAGTTATTG -3'
(R):5'- ACGCTGACCATCCTCAGTTCTTATG -3'

Posted On

2016-10-05