Mutagenetix > Incidental Mutation

Incidental Mutation 'R5517:Apob'

431366

Institutional Source

Beutler Lab

Gene Symbol

Apob

Ensembl Gene

ENSMUSG00000020609

Gene Name

apolipoprotein B

Synonyms

apob-100, apob-48

MMRRC Submission

043076-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.886) question?

Stock #

R5517 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

7977648-8016835 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 7990906 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 664 (L664P)

Ref Sequence

ENSEMBL: ENSMUSP00000035761 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037520] [ENSMUST00000037811] [ENSMUST00000171271]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000037520
AA Change: L664P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035761
Gene: ENSMUSG00000020609
AA Change: L664P

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	33	585	6.03e-94	SMART
DUF1943	619	932	7.88e-97	SMART
Pfam:DUF1081	945	1059	9.4e-32	PFAM
low complexity region	1100	1109	N/A	INTRINSIC
Blast:LPD_N	1249	1311	9e-22	BLAST
low complexity region	1632	1644	N/A	INTRINSIC
internal_repeat_1	1882	2038	6.61e-9	PROSPERO
SCOP:d1gw5a_	2105	2577	9e-5	SMART
internal_repeat_1	2973	3150	6.61e-9	PROSPERO
low complexity region	3561	3580	N/A	INTRINSIC
low complexity region	3928	3936	N/A	INTRINSIC
Pfam:ApoB100_C	4401	4456	5.6e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000037811
AA Change: L677P

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000036044
Gene: ENSMUSG00000020609
AA Change: L677P

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
LPD_N	46	598	6.03e-94	SMART
DUF1943	632	945	7.88e-97	SMART
Pfam:DUF1081	960	1070	6.3e-39	PFAM
low complexity region	1113	1122	N/A	INTRINSIC
Blast:LPD_N	1282	1344	1e-21	BLAST
low complexity region	1665	1677	N/A	INTRINSIC
internal_repeat_1	1915	2071	6.6e-9	PROSPERO
SCOP:d1gw5a_	2138	2610	9e-5	SMART
internal_repeat_1	3006	3183	6.6e-9	PROSPERO
low complexity region	3594	3613	N/A	INTRINSIC
low complexity region	3961	3969	N/A	INTRINSIC
Pfam:ApoB100_C	4434	4490	1.6e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171271

SMART Domains

Protein: ENSMUSP00000127147
Gene: ENSMUSG00000020609

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
Pfam:Vitellogenin_N	45	324	6.7e-53	PFAM

Meta Mutation Damage Score

0.8025

Coding Region Coverage

1x: 98.5%
3x: 97.4%
10x: 95.2%
20x: 90.5%

Validation Efficiency

99% (67/68)

MGI Phenotype

FUNCTION: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mutants usually die by midgestation and longer survivors exhibit exencephaly. Heterozygotes show reduced plasma cholesterol and apolipoprotein levels. Single isoform B100 and B48 null mutants are viable. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf1	C	T	17: 35,958,341 (GRCm38)	R675K	possibly damaging	Het
Aire	A	T	10: 78,039,691 (GRCm38)	S282T	probably benign	Het
Ak9	A	G	10: 41,340,891 (GRCm38)	E283G	probably benign	Het
Akap2	T	C	4: 57,855,987 (GRCm38)	Y439H	probably damaging	Het
Akap9	T	A	5: 4,001,665 (GRCm38)	D1477E	possibly damaging	Het
Ap2a1	T	C	7: 44,906,981 (GRCm38)	D273G	possibly damaging	Het
Arhgap35	A	T	7: 16,563,489 (GRCm38)	F550L	probably damaging	Het
Armc2	T	C	10: 41,963,850 (GRCm38)	E373G	probably benign	Het
Atp8b2	C	A	3: 89,946,031 (GRCm38)	A726S	probably benign	Het
C030048H21Rik	T	A	2: 26,255,887 (GRCm38)	Q87L	probably damaging	Het
Cd244	T	A	1: 171,577,974 (GRCm38)		probably benign	Het
Cdk10	T	A	8: 123,230,587 (GRCm38)		probably null	Het
Cenpe	C	A	3: 135,223,265 (GRCm38)	P310Q	probably damaging	Het
Chuk	T	A	19: 44,097,533 (GRCm38)		probably null	Het
Crebl2	T	C	6: 134,851,176 (GRCm38)	S104P	probably benign	Het
Ddo	A	G	10: 40,647,730 (GRCm38)	K239E	probably benign	Het
Defb5	A	G	8: 19,250,852 (GRCm38)		probably null	Het
Dhx35	T	A	2: 158,834,912 (GRCm38)	M422K	probably damaging	Het
Fchsd1	C	T	18: 37,959,873 (GRCm38)		probably benign	Het
Gatm	G	A	2: 122,595,543 (GRCm38)	T409I	probably damaging	Het
Gdpd1	A	T	11: 87,059,506 (GRCm38)	D80E	probably damaging	Het
Gspt1	C	A	16: 11,253,979 (GRCm38)	G7C	unknown	Het
Hells	G	A	19: 38,954,800 (GRCm38)	S516N	probably benign	Het
Ints1	A	G	5: 139,752,787 (GRCm38)	S2069P	possibly damaging	Het
Kank4	G	A	4: 98,774,881 (GRCm38)	T690M	probably damaging	Het
Kcnq4	T	C	4: 120,715,809 (GRCm38)	N265S	possibly damaging	Het
Kif5b	C	A	18: 6,220,954 (GRCm38)	A385S	probably benign	Het
Map2	T	C	1: 66,415,256 (GRCm38)	S1102P	probably benign	Het
Mcm7	A	G	5: 138,164,871 (GRCm38)	S340P	possibly damaging	Het
Mcrs1	C	T	15: 99,246,995 (GRCm38)	R246H	possibly damaging	Het
Myo16	T	A	8: 10,560,226 (GRCm38)	M1189K	probably benign	Het
Olfr1302	G	T	2: 111,780,459 (GRCm38)	M46I	probably benign	Het
Olfr463	A	T	11: 87,893,066 (GRCm38)	I286N	probably damaging	Het
Olfr847	G	A	9: 19,375,767 (GRCm38)	T38I	probably damaging	Het
Otog	A	G	7: 46,274,571 (GRCm38)	N1118S	probably damaging	Het
Pcdhb7	G	T	18: 37,341,793 (GRCm38)		probably benign	Het
Picalm	C	T	7: 90,170,598 (GRCm38)	T189I	possibly damaging	Het
Ptx4	T	A	17: 25,124,786 (GRCm38)	S337T	possibly damaging	Het
Rad51ap2	C	T	12: 11,458,312 (GRCm38)	S745L	probably benign	Het
Rspry1	G	A	8: 94,636,760 (GRCm38)		probably null	Het
Scn5a	T	G	9: 119,495,713 (GRCm38)	I1350L	probably damaging	Het
Sgk2	T	C	2: 162,997,835 (GRCm38)	L121P	probably damaging	Het
Slc17a1	A	T	13: 23,872,592 (GRCm38)		probably benign	Het
Slc6a12	A	G	6: 121,354,339 (GRCm38)	N183S	probably benign	Het
Smg9	C	A	7: 24,414,913 (GRCm38)		probably benign	Het
Spred1	G	A	2: 117,177,714 (GRCm38)	S367N	probably damaging	Het
Srpr	T	C	9: 35,211,350 (GRCm38)	V21A	probably benign	Het
Taar2	A	T	10: 23,940,729 (GRCm38)	I56F	possibly damaging	Het
Taf1a	T	G	1: 183,395,985 (GRCm38)	L67R	probably damaging	Het
Tbc1d10b	C	A	7: 127,198,607 (GRCm38)	R787S	possibly damaging	Het
Topbp1	T	A	9: 103,336,114 (GRCm38)	N1044K	probably benign	Het
Usp24	A	G	4: 106,375,674 (GRCm38)	T886A	probably benign	Het
Vmn2r26	A	G	6: 124,050,717 (GRCm38)	D472G	probably damaging	Het
Zyg11a	T	C	4: 108,204,746 (GRCm38)	N286S	possibly damaging	Het

Other mutations in Apob

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00402:Apob	APN	12	7,993,065 (GRCm38)	splice site	probably benign
IGL00421:Apob	APN	12	8,010,197 (GRCm38)	missense	probably damaging	0.99
IGL00658:Apob	APN	12	8,009,471 (GRCm38)	missense	probably benign	0.08
IGL00768:Apob	APN	12	8,002,107 (GRCm38)	missense	probably damaging	1.00
IGL00833:Apob	APN	12	8,010,101 (GRCm38)	missense	probably benign	0.14
IGL00926:Apob	APN	12	8,015,421 (GRCm38)	missense	probably benign	0.01
IGL01065:Apob	APN	12	8,003,299 (GRCm38)	missense	probably damaging	0.99
IGL01313:Apob	APN	12	8,000,898 (GRCm38)	missense	probably damaging	1.00
IGL01419:Apob	APN	12	8,002,251 (GRCm38)	missense	probably damaging	0.99
IGL01461:Apob	APN	12	8,001,884 (GRCm38)	missense	probably benign	0.13
IGL02002:Apob	APN	12	7,994,822 (GRCm38)	missense	probably benign	0.03
IGL02031:Apob	APN	12	8,015,222 (GRCm38)	missense	probably benign
IGL02102:Apob	APN	12	7,989,407 (GRCm38)	missense	possibly damaging	0.94
IGL02115:Apob	APN	12	7,992,923 (GRCm38)	missense	probably benign	0.06
IGL02513:Apob	APN	12	7,992,979 (GRCm38)	missense	probably benign	0.01
IGL02967:Apob	APN	12	8,015,366 (GRCm38)	nonsense	probably null
IGL03005:Apob	APN	12	7,993,059 (GRCm38)	splice site	probably benign
IGL03011:Apob	APN	12	7,997,883 (GRCm38)	missense	probably damaging	1.00
IGL03116:Apob	APN	12	8,016,350 (GRCm38)	missense	probably damaging	0.98
IGL03215:Apob	APN	12	8,013,818 (GRCm38)	missense	possibly damaging	0.92
IGL03227:Apob	APN	12	8,016,089 (GRCm38)	missense	probably benign	0.04
Aesthete	UTSW	12	8,010,080 (GRCm38)	nonsense	probably null
Essence	UTSW	12	8,007,769 (GRCm38)	nonsense	probably null
Ethos	UTSW	12	7,990,394 (GRCm38)	missense	probably null	1.00
IGL02835:Apob	UTSW	12	8,015,097 (GRCm38)	missense	possibly damaging	0.86
IGL02837:Apob	UTSW	12	8,005,102 (GRCm38)	missense	probably damaging	1.00
R0071:Apob	UTSW	12	8,002,111 (GRCm38)	missense	probably damaging	0.98
R0071:Apob	UTSW	12	8,002,111 (GRCm38)	missense	probably damaging	0.98
R0116:Apob	UTSW	12	7,989,113 (GRCm38)	unclassified	probably benign
R0180:Apob	UTSW	12	8,008,285 (GRCm38)	nonsense	probably null
R0288:Apob	UTSW	12	7,990,779 (GRCm38)	nonsense	probably null
R0295:Apob	UTSW	12	8,002,181 (GRCm38)	nonsense	probably null
R0305:Apob	UTSW	12	8,012,210 (GRCm38)	missense	probably damaging	1.00
R0312:Apob	UTSW	12	8,009,034 (GRCm38)	missense	probably benign
R0324:Apob	UTSW	12	8,010,521 (GRCm38)	missense	probably benign	0.41
R0326:Apob	UTSW	12	7,990,307 (GRCm38)	missense	probably damaging	1.00
R0363:Apob	UTSW	12	8,010,136 (GRCm38)	missense	probably damaging	1.00
R0390:Apob	UTSW	12	7,988,678 (GRCm38)	missense	probably damaging	0.99
R0462:Apob	UTSW	12	8,000,896 (GRCm38)	missense	probably damaging	1.00
R0471:Apob	UTSW	12	7,990,406 (GRCm38)	missense	probably damaging	1.00
R0532:Apob	UTSW	12	8,016,188 (GRCm38)	missense	possibly damaging	0.48
R0548:Apob	UTSW	12	8,006,282 (GRCm38)	missense	probably damaging	1.00
R0560:Apob	UTSW	12	8,005,101 (GRCm38)	missense	probably damaging	1.00
R0595:Apob	UTSW	12	8,008,369 (GRCm38)	missense	probably benign	0.01
R0600:Apob	UTSW	12	8,006,440 (GRCm38)	missense	probably damaging	1.00
R0626:Apob	UTSW	12	8,016,193 (GRCm38)	missense	probably benign	0.45
R0685:Apob	UTSW	12	8,010,742 (GRCm38)	missense	probably benign
R0765:Apob	UTSW	12	8,016,518 (GRCm38)	missense	probably benign
R0790:Apob	UTSW	12	8,010,245 (GRCm38)	missense	probably damaging	1.00
R0918:Apob	UTSW	12	7,983,941 (GRCm38)	missense	probably benign	0.10
R0962:Apob	UTSW	12	7,989,191 (GRCm38)	missense	probably damaging	0.98
R1055:Apob	UTSW	12	7,994,963 (GRCm38)	missense	probably damaging	1.00
R1077:Apob	UTSW	12	8,006,017 (GRCm38)	missense	probably benign
R1143:Apob	UTSW	12	8,012,354 (GRCm38)	missense	probably benign	0.26
R1163:Apob	UTSW	12	8,011,654 (GRCm38)	missense	probably damaging	1.00
R1266:Apob	UTSW	12	8,006,093 (GRCm38)	missense	probably benign	0.37
R1434:Apob	UTSW	12	8,009,715 (GRCm38)	missense	probably damaging	1.00
R1442:Apob	UTSW	12	7,986,165 (GRCm38)	missense	probably benign	0.31
R1445:Apob	UTSW	12	8,016,084 (GRCm38)	missense	possibly damaging	0.48
R1459:Apob	UTSW	12	8,011,937 (GRCm38)	missense	possibly damaging	0.92
R1459:Apob	UTSW	12	8,006,047 (GRCm38)	missense	probably benign
R1465:Apob	UTSW	12	8,011,421 (GRCm38)	missense	possibly damaging	0.91
R1465:Apob	UTSW	12	8,011,421 (GRCm38)	missense	possibly damaging	0.91
R1508:Apob	UTSW	12	8,011,481 (GRCm38)	missense	possibly damaging	0.92
R1518:Apob	UTSW	12	7,989,207 (GRCm38)	missense	probably benign	0.01
R1531:Apob	UTSW	12	7,997,880 (GRCm38)	missense	possibly damaging	0.65
R1547:Apob	UTSW	12	8,003,368 (GRCm38)	missense	probably benign	0.08
R1574:Apob	UTSW	12	7,990,839 (GRCm38)	missense	possibly damaging	0.51
R1574:Apob	UTSW	12	7,990,839 (GRCm38)	missense	possibly damaging	0.51
R1682:Apob	UTSW	12	8,012,365 (GRCm38)	missense	probably benign	0.00
R1709:Apob	UTSW	12	8,009,306 (GRCm38)	missense	probably damaging	0.98
R1718:Apob	UTSW	12	8,016,087 (GRCm38)	missense	probably benign	0.02
R1752:Apob	UTSW	12	7,988,766 (GRCm38)	missense	probably benign	0.01
R1781:Apob	UTSW	12	8,009,603 (GRCm38)	missense	possibly damaging	0.96
R1818:Apob	UTSW	12	8,013,064 (GRCm38)	missense	possibly damaging	0.93
R1818:Apob	UTSW	12	8,006,834 (GRCm38)	missense	probably damaging	0.98
R1842:Apob	UTSW	12	8,011,559 (GRCm38)	missense	probably damaging	1.00
R1843:Apob	UTSW	12	8,007,602 (GRCm38)	missense	possibly damaging	0.65
R1853:Apob	UTSW	12	8,010,928 (GRCm38)	nonsense	probably null
R1990:Apob	UTSW	12	8,001,039 (GRCm38)	missense	probably damaging	1.00
R2016:Apob	UTSW	12	8,007,751 (GRCm38)	missense	possibly damaging	0.48
R2017:Apob	UTSW	12	8,007,751 (GRCm38)	missense	possibly damaging	0.48
R2023:Apob	UTSW	12	8,011,090 (GRCm38)	missense	probably benign	0.01
R2037:Apob	UTSW	12	8,007,488 (GRCm38)	missense	probably benign	0.37
R2054:Apob	UTSW	12	8,013,134 (GRCm38)	missense	probably damaging	1.00
R2057:Apob	UTSW	12	8,002,164 (GRCm38)	nonsense	probably null
R2085:Apob	UTSW	12	8,012,240 (GRCm38)	missense	probably damaging	1.00
R2159:Apob	UTSW	12	8,010,081 (GRCm38)	missense	probably benign	0.12
R2209:Apob	UTSW	12	8,007,752 (GRCm38)	missense	probably benign	0.28
R2249:Apob	UTSW	12	8,007,499 (GRCm38)	missense	probably damaging	1.00
R2254:Apob	UTSW	12	8,011,256 (GRCm38)	missense	possibly damaging	0.92
R2265:Apob	UTSW	12	8,015,475 (GRCm38)	missense	possibly damaging	0.74
R2266:Apob	UTSW	12	8,015,475 (GRCm38)	missense	possibly damaging	0.74
R2267:Apob	UTSW	12	8,015,475 (GRCm38)	missense	possibly damaging	0.74
R2268:Apob	UTSW	12	8,015,475 (GRCm38)	missense	possibly damaging	0.74
R2296:Apob	UTSW	12	7,994,879 (GRCm38)	missense	probably damaging	0.97
R2897:Apob	UTSW	12	8,010,356 (GRCm38)	missense	probably damaging	1.00
R3431:Apob	UTSW	12	8,010,778 (GRCm38)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,006,327 (GRCm38)	missense	probably damaging	1.00
R3723:Apob	UTSW	12	8,011,763 (GRCm38)	missense	possibly damaging	0.46
R3899:Apob	UTSW	12	8,015,849 (GRCm38)	missense	possibly damaging	0.87
R4020:Apob	UTSW	12	7,994,914 (GRCm38)	nonsense	probably null
R4050:Apob	UTSW	12	8,015,390 (GRCm38)	missense	probably benign	0.02
R4351:Apob	UTSW	12	7,993,054 (GRCm38)	missense	probably benign	0.03
R4365:Apob	UTSW	12	8,016,083 (GRCm38)	missense	possibly damaging	0.95
R4366:Apob	UTSW	12	8,016,083 (GRCm38)	missense	possibly damaging	0.95
R4456:Apob	UTSW	12	8,015,445 (GRCm38)	missense	probably damaging	1.00
R4458:Apob	UTSW	12	8,015,445 (GRCm38)	missense	probably damaging	1.00
R4600:Apob	UTSW	12	8,008,568 (GRCm38)	missense	probably damaging	1.00
R4611:Apob	UTSW	12	8,011,331 (GRCm38)	missense	probably damaging	1.00
R4646:Apob	UTSW	12	8,012,759 (GRCm38)	missense	probably benign	0.21
R4678:Apob	UTSW	12	7,995,585 (GRCm38)	missense	probably damaging	1.00
R4685:Apob	UTSW	12	8,006,456 (GRCm38)	missense	probably benign	0.00
R4707:Apob	UTSW	12	8,006,205 (GRCm38)	missense	probably damaging	0.96
R4726:Apob	UTSW	12	7,990,267 (GRCm38)	missense	probably damaging	0.98
R4792:Apob	UTSW	12	8,008,051 (GRCm38)	missense	probably benign	0.26
R4822:Apob	UTSW	12	8,015,741 (GRCm38)	missense	probably benign	0.04
R4834:Apob	UTSW	12	8,014,101 (GRCm38)	missense	possibly damaging	0.49
R4835:Apob	UTSW	12	8,015,391 (GRCm38)	missense	possibly damaging	0.56
R4887:Apob	UTSW	12	8,013,099 (GRCm38)	missense	probably damaging	1.00
R4910:Apob	UTSW	12	8,007,848 (GRCm38)	missense	probably damaging	1.00
R5072:Apob	UTSW	12	8,008,714 (GRCm38)	missense	probably benign	0.00
R5073:Apob	UTSW	12	8,005,219 (GRCm38)	critical splice donor site	probably null
R5074:Apob	UTSW	12	8,005,219 (GRCm38)	critical splice donor site	probably null
R5101:Apob	UTSW	12	8,011,934 (GRCm38)	missense	probably benign	0.09
R5123:Apob	UTSW	12	8,007,630 (GRCm38)	splice site	probably null
R5133:Apob	UTSW	12	8,008,898 (GRCm38)	missense	probably damaging	0.99
R5135:Apob	UTSW	12	8,010,086 (GRCm38)	missense	probably damaging	1.00
R5137:Apob	UTSW	12	8,011,384 (GRCm38)	missense	possibly damaging	0.63
R5160:Apob	UTSW	12	8,012,126 (GRCm38)	missense	possibly damaging	0.90
R5173:Apob	UTSW	12	8,008,238 (GRCm38)	missense	probably benign	0.00
R5202:Apob	UTSW	12	8,013,737 (GRCm38)	missense	probably damaging	0.98
R5229:Apob	UTSW	12	7,977,806 (GRCm38)	missense	probably benign
R5292:Apob	UTSW	12	8,005,912 (GRCm38)	missense	probably benign	0.01
R5378:Apob	UTSW	12	8,011,865 (GRCm38)	missense	probably damaging	0.99
R5494:Apob	UTSW	12	8,011,762 (GRCm38)	missense	probably damaging	0.99
R5576:Apob	UTSW	12	7,998,662 (GRCm38)	missense	probably damaging	1.00
R5582:Apob	UTSW	12	8,010,788 (GRCm38)	missense	probably damaging	1.00
R5629:Apob	UTSW	12	8,007,847 (GRCm38)	missense	probably damaging	1.00
R5678:Apob	UTSW	12	7,991,494 (GRCm38)	missense	possibly damaging	0.92
R5732:Apob	UTSW	12	8,010,353 (GRCm38)	missense	probably benign	0.15
R5734:Apob	UTSW	12	7,988,781 (GRCm38)	missense	probably damaging	1.00
R5742:Apob	UTSW	12	8,007,191 (GRCm38)	missense	probably damaging	1.00
R5751:Apob	UTSW	12	8,012,619 (GRCm38)	nonsense	probably null
R5776:Apob	UTSW	12	8,006,149 (GRCm38)	missense	possibly damaging	0.57
R5778:Apob	UTSW	12	8,015,074 (GRCm38)	missense	probably benign	0.45
R5783:Apob	UTSW	12	8,001,022 (GRCm38)	missense	probably damaging	1.00
R5786:Apob	UTSW	12	8,015,304 (GRCm38)	missense	possibly damaging	0.48
R5837:Apob	UTSW	12	8,003,277 (GRCm38)	missense	probably benign	0.04
R5857:Apob	UTSW	12	8,015,397 (GRCm38)	missense	probably benign	0.00
R6029:Apob	UTSW	12	8,016,243 (GRCm38)	missense	probably damaging	0.99
R6032:Apob	UTSW	12	7,995,513 (GRCm38)	missense	probably benign	0.02
R6032:Apob	UTSW	12	7,995,513 (GRCm38)	missense	probably benign	0.02
R6086:Apob	UTSW	12	8,015,164 (GRCm38)	missense	probably benign
R6110:Apob	UTSW	12	8,011,883 (GRCm38)	missense	probably damaging	1.00
R6131:Apob	UTSW	12	8,015,874 (GRCm38)	missense	probably benign	0.17
R6157:Apob	UTSW	12	8,006,077 (GRCm38)	missense	probably benign
R6179:Apob	UTSW	12	8,005,060 (GRCm38)	nonsense	probably null
R6247:Apob	UTSW	12	8,001,801 (GRCm38)	missense	probably damaging	1.00
R6279:Apob	UTSW	12	8,007,769 (GRCm38)	nonsense	probably null
R6300:Apob	UTSW	12	8,007,769 (GRCm38)	nonsense	probably null
R6320:Apob	UTSW	12	7,989,194 (GRCm38)	missense	probably benign	0.27
R6339:Apob	UTSW	12	8,016,188 (GRCm38)	missense	probably damaging	0.99
R6353:Apob	UTSW	12	8,009,421 (GRCm38)	missense	probably damaging	1.00
R6395:Apob	UTSW	12	8,008,507 (GRCm38)	missense	probably benign	0.45
R6441:Apob	UTSW	12	7,987,796 (GRCm38)	missense	probably damaging	1.00
R6492:Apob	UTSW	12	8,008,261 (GRCm38)	missense	probably damaging	0.99
R6495:Apob	UTSW	12	7,990,394 (GRCm38)	missense	probably null	1.00
R6502:Apob	UTSW	12	8,001,814 (GRCm38)	missense	probably damaging	0.99
R6520:Apob	UTSW	12	7,983,124 (GRCm38)	missense	probably damaging	1.00
R6644:Apob	UTSW	12	8,009,077 (GRCm38)	missense	probably damaging	0.97
R6704:Apob	UTSW	12	8,010,379 (GRCm38)	missense	probably damaging	0.98
R6750:Apob	UTSW	12	7,997,853 (GRCm38)	missense	probably damaging	1.00
R6759:Apob	UTSW	12	8,011,049 (GRCm38)	missense	probably benign	0.06
R6812:Apob	UTSW	12	7,983,062 (GRCm38)	missense	probably damaging	0.98
R6865:Apob	UTSW	12	8,008,847 (GRCm38)	missense	probably benign	0.05
R6873:Apob	UTSW	12	8,015,995 (GRCm38)	missense	probably benign	0.00
R7013:Apob	UTSW	12	8,010,080 (GRCm38)	nonsense	probably null
R7067:Apob	UTSW	12	8,009,423 (GRCm38)	missense	probably damaging	1.00
R7084:Apob	UTSW	12	8,009,591 (GRCm38)	missense	probably benign
R7113:Apob	UTSW	12	7,995,539 (GRCm38)	missense	probably damaging	1.00
R7175:Apob	UTSW	12	8,007,034 (GRCm38)	missense	probably benign	0.33
R7196:Apob	UTSW	12	7,983,893 (GRCm38)	missense	possibly damaging	0.90
R7199:Apob	UTSW	12	8,005,072 (GRCm38)	missense	probably damaging	1.00
R7205:Apob	UTSW	12	8,005,087 (GRCm38)	missense	probably damaging	0.98
R7251:Apob	UTSW	12	8,007,037 (GRCm38)	missense	probably damaging	0.98
R7474:Apob	UTSW	12	8,009,185 (GRCm38)	missense	probably benign	0.29
R7484:Apob	UTSW	12	8,006,884 (GRCm38)	nonsense	probably null
R7538:Apob	UTSW	12	8,002,219 (GRCm38)	missense	probably damaging	0.98
R7636:Apob	UTSW	12	8,009,516 (GRCm38)	missense	possibly damaging	0.86
R7646:Apob	UTSW	12	8,009,189 (GRCm38)	missense	probably damaging	0.99
R7787:Apob	UTSW	12	7,990,780 (GRCm38)	missense	probably damaging	0.97
R7793:Apob	UTSW	12	8,008,124 (GRCm38)	missense	probably damaging	0.99
R7836:Apob	UTSW	12	8,001,885 (GRCm38)	missense	possibly damaging	0.72
R7895:Apob	UTSW	12	8,011,933 (GRCm38)	missense	probably benign	0.00
R8005:Apob	UTSW	12	8,009,744 (GRCm38)	missense	probably benign	0.01
R8013:Apob	UTSW	12	8,010,798 (GRCm38)	missense	possibly damaging	0.94
R8014:Apob	UTSW	12	8,010,798 (GRCm38)	missense	possibly damaging	0.94
R8111:Apob	UTSW	12	8,008,801 (GRCm38)	missense	probably benign	0.16
R8117:Apob	UTSW	12	8,006,435 (GRCm38)	missense	probably damaging	0.99
R8226:Apob	UTSW	12	8,009,056 (GRCm38)	missense	probably benign	0.00
R8244:Apob	UTSW	12	8,010,548 (GRCm38)	missense	probably damaging	0.96
R8280:Apob	UTSW	12	8,010,851 (GRCm38)	missense	possibly damaging	0.46
R8310:Apob	UTSW	12	8,009,033 (GRCm38)	missense	probably benign	0.00
R8327:Apob	UTSW	12	8,001,015 (GRCm38)	missense	possibly damaging	0.72
R8329:Apob	UTSW	12	8,011,135 (GRCm38)	missense	probably damaging	0.98
R8331:Apob	UTSW	12	8,001,882 (GRCm38)	missense	probably benign	0.28
R8351:Apob	UTSW	12	8,006,356 (GRCm38)	missense	probably benign	0.29
R8412:Apob	UTSW	12	8,008,069 (GRCm38)	missense	probably benign	0.33
R8425:Apob	UTSW	12	7,988,842 (GRCm38)	missense	possibly damaging	0.70
R8481:Apob	UTSW	12	7,994,807 (GRCm38)	splice site	probably null
R8493:Apob	UTSW	12	8,009,009 (GRCm38)	missense	possibly damaging	0.87
R8529:Apob	UTSW	12	8,007,353 (GRCm38)	missense	probably damaging	1.00
R8554:Apob	UTSW	12	7,987,830 (GRCm38)	missense	probably damaging	0.98
R8692:Apob	UTSW	12	8,008,270 (GRCm38)	missense	probably damaging	0.98
R8695:Apob	UTSW	12	8,007,830 (GRCm38)	missense	probably damaging	1.00
R8977:Apob	UTSW	12	8,015,990 (GRCm38)	missense	probably damaging	0.99
R9016:Apob	UTSW	12	7,985,408 (GRCm38)	splice site	silent
R9020:Apob	UTSW	12	8,013,999 (GRCm38)	missense	probably damaging	1.00
R9037:Apob	UTSW	12	8,016,501 (GRCm38)	missense	probably benign	0.15
R9053:Apob	UTSW	12	8,008,954 (GRCm38)	missense	possibly damaging	0.72
R9062:Apob	UTSW	12	8,008,046 (GRCm38)	missense	possibly damaging	0.91
R9142:Apob	UTSW	12	8,012,705 (GRCm38)	missense	possibly damaging	0.95
R9180:Apob	UTSW	12	7,997,925 (GRCm38)	missense	probably damaging	1.00
R9205:Apob	UTSW	12	7,980,635 (GRCm38)	missense	probably damaging	0.99
R9248:Apob	UTSW	12	8,015,231 (GRCm38)	nonsense	probably null
R9277:Apob	UTSW	12	8,011,183 (GRCm38)	missense	probably benign	0.01
R9305:Apob	UTSW	12	8,008,053 (GRCm38)	missense	probably benign	0.04
R9358:Apob	UTSW	12	8,010,833 (GRCm38)	missense	probably benign	0.14
R9375:Apob	UTSW	12	7,979,261 (GRCm38)	missense	possibly damaging	0.91
R9385:Apob	UTSW	12	8,006,399 (GRCm38)	missense	possibly damaging	0.91
R9386:Apob	UTSW	12	8,006,629 (GRCm38)	missense	probably damaging	0.99
R9392:Apob	UTSW	12	8,007,098 (GRCm38)	missense	probably benign	0.45
R9470:Apob	UTSW	12	7,989,219 (GRCm38)	missense	possibly damaging	0.94
R9523:Apob	UTSW	12	8,002,069 (GRCm38)	missense	probably damaging	1.00
R9545:Apob	UTSW	12	7,983,890 (GRCm38)	missense	possibly damaging	0.81
R9629:Apob	UTSW	12	8,009,054 (GRCm38)	missense	probably damaging	1.00
R9702:Apob	UTSW	12	8,007,559 (GRCm38)	missense	probably damaging	0.96
R9703:Apob	UTSW	12	7,980,507 (GRCm38)	missense	probably damaging	0.99
R9719:Apob	UTSW	12	8,015,464 (GRCm38)	missense	probably benign	0.15
R9726:Apob	UTSW	12	8,006,926 (GRCm38)	missense	probably damaging	0.99
R9729:Apob	UTSW	12	8,016,125 (GRCm38)	missense	probably damaging	0.99
X0027:Apob	UTSW	12	8,007,975 (GRCm38)	missense	probably benign
Z1088:Apob	UTSW	12	8,012,936 (GRCm38)	missense	possibly damaging	0.95
Z1088:Apob	UTSW	12	8,005,945 (GRCm38)	nonsense	probably null
Z1088:Apob	UTSW	12	8,005,074 (GRCm38)	missense	possibly damaging	0.91
Z1176:Apob	UTSW	12	8,004,978 (GRCm38)	missense	probably benign	0.00
Z1176:Apob	UTSW	12	7,998,011 (GRCm38)	missense	probably damaging	1.00
Z1177:Apob	UTSW	12	8,015,249 (GRCm38)	frame shift	probably null
Z1177:Apob	UTSW	12	7,988,765 (GRCm38)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- TCTGGAGAATTCTCAATTTCCAACG -3'
(R):5'- TCAGAGTTGGGATCAGATGCAG -3'

Sequencing Primer
(F):5'- TTCCAACGATCATGGACTTCAG -3'
(R):5'- GAGGAATCATGTGGAGACATTTTTCC -3'

Posted On

2016-10-05