Mutagenetix > Incidental Mutations

Incidental Mutation 'R5526:Tmem225'

431862

Institutional Source

Beutler Lab

Gene Symbol

Tmem225

Ensembl Gene

ENSMUSG00000040541

Gene Name

transmembrane protein 225

Synonyms

MMRRC Submission

043084-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R5526 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

40148122-40150880 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 40150706 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 205 (H205Q)

Ref Sequence

ENSEMBL: ENSMUSP00000036816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046333]

AlphaFold

Q9D9S2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000046333
AA Change: H205Q

PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000036816
Gene: ENSMUSG00000040541
AA Change: H205Q

Domain	Start	End	E-Value	Type
transmembrane domain	12	34	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	97	119	N/A	INTRINSIC
transmembrane domain	139	161	N/A	INTRINSIC

Coding Region Coverage

1x: 98.3%
3x: 97.3%
10x: 95.2%
20x: 90.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700022I11Rik	T	C	4: 42,972,125	V486A	possibly damaging	Het
Abca12	A	T	1: 71,292,446	M1315K	probably benign	Het
Abcc4	C	T	14: 118,631,037	V168I	probably benign	Het
Add3	T	A	19: 53,226,607	L71Q	probably damaging	Het
Agbl5	G	A	5: 30,893,903		probably null	Het
Angpt1	A	G	15: 42,512,341	L173P	probably damaging	Het
BC067074	T	C	13: 113,367,893	V1852A	probably benign	Het
Crls1	T	A	2: 132,861,245	M205K	possibly damaging	Het
Dhtkd1	A	T	2: 5,911,851	N671K	probably damaging	Het
Dmbt1	A	T	7: 131,041,190	D246V	probably damaging	Het
Dolk	G	A	2: 30,285,808	A75V	probably damaging	Het
Dtna	T	A	18: 23,646,230	V623E	probably damaging	Het
Elavl3	T	C	9: 22,036,326	T106A	probably benign	Het
Ergic1	T	C	17: 26,624,678	C41R	probably damaging	Het
Fat2	T	A	11: 55,269,361	I3309F	possibly damaging	Het
Fbn1	C	A	2: 125,365,639	R978L	possibly damaging	Het
Fzd6	A	T	15: 39,031,164	S242C	possibly damaging	Het
Galntl6	T	A	8: 58,472,970	H87L	probably benign	Het
Gatad2a	T	C	8: 69,935,941	E32G	probably damaging	Het
Gemin6	T	G	17: 80,227,749	V46G	probably damaging	Het
Gm11541	G	T	11: 94,704,118	H41Q	unknown	Het
Has3	A	G	8: 106,873,947	T14A	probably damaging	Het
Kmt2b	A	G	7: 30,580,444	L1377P	probably damaging	Het
Lcorl	T	C	5: 45,733,727	N428S	probably benign	Het
Lipo5	A	T	19: 33,467,884	V95D	unknown	Het
Lrp1	A	T	10: 127,555,724	V2942D	probably benign	Het
Map1a	T	C	2: 121,305,662	S2082P	probably damaging	Het
Mast4	T	C	13: 102,754,215	S852G	possibly damaging	Het
Mlh3	A	T	12: 85,269,373	L13*	probably null	Het
Ncam2	C	T	16: 81,434,878	R77*	probably null	Het
Nlrp9c	T	A	7: 26,382,366	N645I	possibly damaging	Het
Olfr109	T	A	17: 37,467,112	L302Q	unknown	Het
Olfr1126	T	C	2: 87,457,765	I200T	probably benign	Het
Olfr128	T	A	17: 37,924,492	*309K	probably null	Het
Olfr857	C	A	9: 19,713,698	Y290*	probably null	Het
Olfr965	A	T	9: 39,719,596	Y123F	possibly damaging	Het
Pex12	A	G	11: 83,296,264	V286A	possibly damaging	Het
Phax	C	T	18: 56,584,310	T275I	probably damaging	Het
Phf21b	A	G	15: 84,791,805	V335A	probably benign	Het
Psg18	A	T	7: 18,349,348	L173H	probably damaging	Het
Rab3ip	T	C	10: 116,918,929	T209A	possibly damaging	Het
Ralgapb	T	C	2: 158,432,785	V202A	probably damaging	Het
Rpn2	T	C	2: 157,323,267	L611P	probably damaging	Het
Runx2	T	G	17: 44,724,862	T148P	probably damaging	Het
Sbpl	C	A	17: 23,954,649	D50Y	probably damaging	Het
Scn5a	G	A	9: 119,521,171	P879L	probably damaging	Het
Sfn	T	C	4: 133,601,604	R56G	probably damaging	Het
Tas2r138	C	T	6: 40,612,980	A111T	probably benign	Het
Tasp1	A	T	2: 140,008,789	S105T	probably damaging	Het
Tmprss7	A	G	16: 45,660,904	S640P	probably damaging	Het
Utp4	G	A	8: 106,917,633	A535T	possibly damaging	Het
Vmn1r203	A	T	13: 22,524,103	D18V	probably benign	Het
Vmn2r58	A	G	7: 41,872,645	L9P	probably benign	Het
Zcchc2	T	A	1: 106,030,254	C420*	probably null	Het
Zfp804a	A	G	2: 82,258,590	D921G	probably benign	Het

Other mutations in Tmem225

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01833:Tmem225	APN	9	40148429	missense	probably damaging	0.98
IGL03094:Tmem225	APN	9	40148386	missense	possibly damaging	0.46
R0599:Tmem225	UTSW	9	40149747	missense	possibly damaging	0.46
R1134:Tmem225	UTSW	9	40149847	missense	possibly damaging	0.92
R2842:Tmem225	UTSW	9	40149801	missense	probably damaging	0.99
R4556:Tmem225	UTSW	9	40149466	missense	probably damaging	0.96
R5024:Tmem225	UTSW	9	40149343	missense	probably benign	0.01
R5540:Tmem225	UTSW	9	40149385	missense	probably damaging	0.99
R5597:Tmem225	UTSW	9	40149430	missense	possibly damaging	0.94
R6267:Tmem225	UTSW	9	40148435	missense	probably damaging	0.97
R6314:Tmem225	UTSW	9	40150720	missense	probably benign
R7153:Tmem225	UTSW	9	40148368	missense	probably benign	0.01
R8031:Tmem225	UTSW	9	40149393	missense	possibly damaging	0.46
R8245:Tmem225	UTSW	9	40150659	missense	probably damaging	0.99
X0064:Tmem225	UTSW	9	40149786	missense	possibly damaging	0.85

Predicted Primers

PCR Primer
(F):5'- TGCATAGTACACTAATCCTGCTCCC -3'
(R):5'- ACTGGTATAGAGTTAACAGTGTAGC -3'

Sequencing Primer
(F):5'- CTTGCTTCTCTGTCCCTAGG -3'
(R):5'- CAACGTCAGGTAGTACTGA -3'

Posted On

2016-10-05