|Institutional Source||Beutler Lab|
|Gene Name||hemolytic complement|
|Synonyms||He, C5, C5a|
|Essential gene?||Possibly non essential (E-score: 0.487)|
|Stock #||R5494 (G1)|
|Chromosomal Location||34983331-35061438 bp(-) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||A to T at 35003539 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000028233 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000028233]|
Crystal structure of the mouse C5a anaphylatoxin [X-RAY DIFFRACTION]
Crystal structure of the mouse C5a-desArg anaphylatoxin [X-RAY DIFFRACTION]
|Coding Region Coverage||
FUNCTION: This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mice with a homozygous mutation in this gene exhibit impaired bone fracture healing and an enhanced inflammatory response in an allergic lung disease model. [provided by RefSeq, Nov 2015]
PHENOTYPE: Macrophage from mice homozygous for disruptions of this gene do not secrete complement C5.
The 2 bp deletion found in A/J and AKR/J strains is associated with susceptibility to allergen-induced bronchial hyperresponsiveness and is a candidate for QTL Abhr2.[provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Hc||
(F):5'- TTACCTGGGTGGAATAAAAGCCG -3'
(R):5'- TGCTCCACTGTCAGACCATG -3'
(F):5'- CTCTTCAGATAGCCACTTGATGATGG -3'
(R):5'- GTGTGGCCATCCTTCTACAATGG -3'