Mutagenetix > Incidental Mutation

Incidental Mutation 'R5495:Galnt15'

432306

Institutional Source

Beutler Lab

Gene Symbol

Galnt15

Ensembl Gene

ENSMUSG00000021903

Gene Name

polypeptide N-acetylgalactosaminyltransferase 15

Synonyms

Galntl2, 4631401E18Rik

MMRRC Submission

043056-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

R5495 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

31750946-31784154 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 31751774 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 109 (S109G)

Ref Sequence

ENSEMBL: ENSMUSP00000022460 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022460] [ENSMUST00000164208]

AlphaFold

Q9D2N8

Predicted Effect

probably damaging
Transcript: ENSMUST00000022460
AA Change: S109G

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000022460
Gene: ENSMUSG00000021903
AA Change: S109G

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	105	116	N/A	INTRINSIC
low complexity region	166	180	N/A	INTRINSIC
Pfam:Glyco_tranf_2_3	191	436	2e-8	PFAM
Pfam:Glycos_transf_2	194	362	9e-32	PFAM
RICIN	505	630	1.19e-6	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000164208
AA Change: S109G

SMART Domains

Protein: ENSMUSP00000131978
Gene: ENSMUSG00000021903
AA Change: S109G

Domain	Start	End	E-Value	Type
transmembrane domain	13	35	N/A	INTRINSIC
low complexity region	105	116	N/A	INTRINSIC
low complexity region	166	180	N/A	INTRINSIC
low complexity region	228	248	N/A	INTRINSIC
RICIN	381	506	1.19e-6	SMART

Coding Region Coverage

1x: 98.4%
3x: 97.3%
10x: 95.3%
20x: 91.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akt2	T	C	7: 27,335,594 (GRCm39)		probably null	Het
Arhgap29	T	G	3: 121,808,578 (GRCm39)	M844R	probably damaging	Het
Atp1a1	T	G	3: 101,498,741 (GRCm39)	D184A	probably benign	Het
Bcl11a	T	A	11: 24,115,042 (GRCm39)	V795E	possibly damaging	Het
Casp12	T	A	9: 5,353,797 (GRCm39)	I277N	possibly damaging	Het
Ccdc59	A	G	10: 105,681,239 (GRCm39)	K164E	probably damaging	Het
D630003M21Rik	C	T	2: 158,062,431 (GRCm39)	G30S	possibly damaging	Het
Dgkd	A	G	1: 87,854,594 (GRCm39)	D632G	probably damaging	Het
Efr3a	A	G	15: 65,687,258 (GRCm39)	K56E	possibly damaging	Het
Egflam	T	A	15: 7,280,722 (GRCm39)	R434S	probably damaging	Het
Fancl	C	G	11: 26,347,801 (GRCm39)	A51G	probably damaging	Het
Fkbp7	T	C	2: 76,493,638 (GRCm39)	Y185C	probably damaging	Het
Galc	A	G	12: 98,197,673 (GRCm39)		probably null	Het
Gramd2b	T	C	18: 56,615,694 (GRCm39)	I163T	probably damaging	Het
Impa1	A	G	3: 10,391,230 (GRCm39)	V80A	probably benign	Het
Itga10	T	C	3: 96,554,687 (GRCm39)	M56T	possibly damaging	Het
Larp1b	G	T	3: 40,990,257 (GRCm39)	R135I	probably damaging	Het
Lgals12	C	T	19: 7,581,495 (GRCm39)	A71T	probably damaging	Het
Lmbr1	A	T	5: 29,551,851 (GRCm39)	L78*	probably null	Het
Lrat	C	A	3: 82,804,289 (GRCm39)	M229I	probably benign	Het
Mug2	A	T	6: 122,056,609 (GRCm39)	M1185L	probably damaging	Het
Naprt	T	C	15: 75,765,696 (GRCm39)		probably null	Het
Nfat5	A	G	8: 108,095,079 (GRCm39)	I1107V	probably benign	Het
Nr4a2	T	C	2: 57,002,387 (GRCm39)	Y22C	probably damaging	Het
Ogfod1	C	A	8: 94,790,906 (GRCm39)	Q526K	probably benign	Het
Or2d4	C	A	7: 106,543,699 (GRCm39)	G170*	probably null	Het
Or3a10	G	A	11: 73,935,611 (GRCm39)	T163I	probably damaging	Het
Or4p21	T	C	2: 88,276,401 (GRCm39)	T294A	probably benign	Het
Or8g36	T	C	9: 39,422,441 (GRCm39)	T192A	probably benign	Het
Parp10	T	G	15: 76,127,366 (GRCm39)	I24L	probably benign	Het
Pcdha11	A	G	18: 37,144,079 (GRCm39)	T57A	probably benign	Het
Prdm8	A	G	5: 98,333,165 (GRCm39)	E244G	possibly damaging	Het
Prl6a1	T	C	13: 27,496,654 (GRCm39)	S3P	possibly damaging	Het
Rab11fip3	T	A	17: 26,235,117 (GRCm39)	T18S	probably damaging	Het
Rfc4	T	C	16: 22,941,004 (GRCm39)		probably benign	Het
Rubcnl	G	T	14: 75,279,777 (GRCm39)	V387F	possibly damaging	Het
S100a7l2	A	T	3: 90,997,602 (GRCm39)	L38M	possibly damaging	Het
Serpinb12	T	C	1: 106,884,151 (GRCm39)	L299P	probably damaging	Het
Sptbn5	G	A	2: 119,876,965 (GRCm39)		probably benign	Het
Taar4	A	T	10: 23,837,181 (GRCm39)	I264F	possibly damaging	Het
Tdpoz4	A	T	3: 93,704,806 (GRCm39)	T368S	probably benign	Het
Thsd7b	T	A	1: 129,523,570 (GRCm39)	H124Q	probably damaging	Het
Ugt1a6a	A	T	1: 88,066,746 (GRCm39)	Q184L	probably benign	Het
Vnn1	T	A	10: 23,774,462 (GRCm39)	F168L	probably damaging	Het
Zan	A	G	5: 137,468,670 (GRCm39)	L267P	probably damaging	Het
Zswim8	A	G	14: 20,772,354 (GRCm39)	S1621G	probably damaging	Het

Other mutations in Galnt15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Galnt15	APN	14	31,774,313 (GRCm39)	missense	possibly damaging	0.47
IGL02491:Galnt15	APN	14	31,778,273 (GRCm39)	missense	probably damaging	1.00
R0323:Galnt15	UTSW	14	31,770,042 (GRCm39)	missense	probably damaging	0.99
R1900:Galnt15	UTSW	14	31,771,822 (GRCm39)	missense	probably damaging	1.00
R4369:Galnt15	UTSW	14	31,751,496 (GRCm39)	missense	possibly damaging	0.90
R4423:Galnt15	UTSW	14	31,780,226 (GRCm39)	missense	possibly damaging	0.95
R4979:Galnt15	UTSW	14	31,765,247 (GRCm39)	missense	probably damaging	0.99
R5093:Galnt15	UTSW	14	31,771,786 (GRCm39)	missense	probably damaging	1.00
R5201:Galnt15	UTSW	14	31,771,822 (GRCm39)	missense	probably damaging	1.00
R5254:Galnt15	UTSW	14	31,780,244 (GRCm39)	nonsense	probably null
R5434:Galnt15	UTSW	14	31,771,800 (GRCm39)	missense	possibly damaging	0.88
R5451:Galnt15	UTSW	14	31,751,868 (GRCm39)	missense	probably benign	0.25
R5874:Galnt15	UTSW	14	31,774,324 (GRCm39)	missense	probably damaging	1.00
R6374:Galnt15	UTSW	14	31,780,116 (GRCm39)	missense	probably damaging	0.96
R6444:Galnt15	UTSW	14	31,762,368 (GRCm39)	missense	probably damaging	1.00
R7798:Galnt15	UTSW	14	31,751,862 (GRCm39)	missense	possibly damaging	0.52
R9007:Galnt15	UTSW	14	31,771,935 (GRCm39)	missense	probably damaging	1.00
R9031:Galnt15	UTSW	14	31,770,027 (GRCm39)	missense	probably damaging	0.99
R9092:Galnt15	UTSW	14	31,780,196 (GRCm39)	missense	probably benign	0.43
R9161:Galnt15	UTSW	14	31,780,116 (GRCm39)	missense	probably damaging	0.96
R9629:Galnt15	UTSW	14	31,774,301 (GRCm39)	missense	probably damaging	1.00
Z1177:Galnt15	UTSW	14	31,774,322 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGGTGATCCTGCTGCATC -3'
(R):5'- TACTTACAGTGGGTGTCGCAC -3'

Sequencing Primer
(F):5'- TGCACCAGGCTGTCACAG -3'
(R):5'- ACTCTCCTCAGGGGCAG -3'

Posted On

2016-10-05