Mutagenetix > Incidental Mutation

Incidental Mutation 'R5485:Or10g3b'

432537

Institutional Source

Beutler Lab

Gene Symbol

Or10g3b

Ensembl Gene

ENSMUSG00000095030

Gene Name

olfactory receptor family 10 subfamily G member 3B

Synonyms

GA_x6K02T2RJGY-644134-645075, Olfr1513, MOR223-10, MOR223-7P

MMRRC Submission

043046-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R5485 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

52586560-52587501 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 52586776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 242 (C242*)

Ref Sequence

ENSEMBL: ENSMUSP00000149216 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089739] [ENSMUST00000215147]

AlphaFold

L7N457

Predicted Effect

probably null
Transcript: ENSMUST00000089739
AA Change: C242*

SMART Domains

Protein: ENSMUSP00000087171
Gene: ENSMUSG00000095030
AA Change: C242*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	5.2e-49	PFAM
Pfam:7tm_1	41	291	1.6e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215147
AA Change: C242*

Meta Mutation Damage Score

0.9718

Coding Region Coverage

1x: 98.2%
3x: 97.3%
10x: 95.1%
20x: 90.6%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700093K21Rik	A	T	11: 23,467,378 (GRCm39)	V85D	probably benign	Het
Abcb8	A	G	5: 24,605,159 (GRCm39)	N115S	probably benign	Het
Appl1	C	A	14: 26,684,823 (GRCm39)	L75F	probably damaging	Het
Atp13a5	A	G	16: 29,100,760 (GRCm39)		probably null	Het
Atp2c2	T	A	8: 120,479,801 (GRCm39)		probably null	Het
Cfap54	T	A	10: 92,864,979 (GRCm39)	I94F	probably damaging	Het
Clasp1	A	G	1: 118,395,643 (GRCm39)	I194V	possibly damaging	Het
Crebbp	A	C	16: 3,932,777 (GRCm39)	D1000E	probably benign	Het
Ctdnep1	C	T	11: 69,872,316 (GRCm39)	R3W	possibly damaging	Het
Dgkb	T	C	12: 38,177,363 (GRCm39)	V230A	probably damaging	Het
Duoxa2	T	C	2: 122,129,633 (GRCm39)	F38L	possibly damaging	Het
Ecd	T	C	14: 20,388,273 (GRCm39)	S172G	probably benign	Het
Endog	G	T	2: 30,061,663 (GRCm39)		probably benign	Het
Eqtn	G	A	4: 94,813,193 (GRCm39)	P131L	possibly damaging	Het
Gabbr1	T	A	17: 37,367,767 (GRCm39)	S290T	possibly damaging	Het
Glt8d2	T	A	10: 82,487,282 (GRCm39)	R319S	possibly damaging	Het
Gm16686	A	G	4: 88,673,641 (GRCm39)		probably benign	Het
Gm5709	A	T	3: 59,542,983 (GRCm39)		noncoding transcript	Het
Gm7381	T	C	8: 3,892,161 (GRCm39)		noncoding transcript	Het
Gorab	T	G	1: 163,213,871 (GRCm39)	D353A	possibly damaging	Het
Gstm1	A	G	3: 107,924,720 (GRCm39)	L20P	probably damaging	Het
Hexim2	A	G	11: 103,029,884 (GRCm39)	D312G	probably benign	Het
Hfm1	A	G	5: 106,995,528 (GRCm39)		probably null	Het
Mbd4	C	A	6: 115,827,679 (GRCm39)	A66S	probably benign	Het
Mmp27	T	A	9: 7,573,363 (GRCm39)	W152R	probably damaging	Het
Mogat1	A	G	1: 78,500,307 (GRCm39)	T124A	probably benign	Het
Mrps18b	A	T	17: 36,225,236 (GRCm39)	V102D	probably damaging	Het
Peg10	T	A	6: 4,755,565 (GRCm39)	M47K	probably benign	Het
Plxnc1	T	G	10: 94,758,604 (GRCm39)	Q364P	probably benign	Het
Psd	A	T	19: 46,304,528 (GRCm39)		probably null	Het
Rims1	T	G	1: 22,522,289 (GRCm39)	I470L	possibly damaging	Het
Sbsn	T	C	7: 30,452,542 (GRCm39)	V519A	possibly damaging	Het
Senp1	A	G	15: 97,964,377 (GRCm39)	V279A	probably benign	Het
Sfxn5	T	A	6: 85,309,582 (GRCm39)		probably benign	Het
Slc6a13	T	A	6: 121,313,032 (GRCm39)	M483K	probably damaging	Het
Slc6a21	G	A	7: 44,931,966 (GRCm39)		probably null	Het
Slco6c1	T	C	1: 97,053,481 (GRCm39)	Y140C	probably damaging	Het
Spata32	A	G	11: 103,100,122 (GRCm39)	S128P	probably damaging	Het
Spty2d1	G	T	7: 46,647,633 (GRCm39)	T432K	possibly damaging	Het
Stx8	A	G	11: 67,911,792 (GRCm39)	Q170R	probably benign	Het
Sytl3	G	A	17: 6,982,879 (GRCm39)	V112I	probably benign	Het
Tdrkh	A	G	3: 94,336,019 (GRCm39)	I420V	probably benign	Het
Tmem117	T	C	15: 94,992,711 (GRCm39)	V457A	probably benign	Het
Tpk1	T	C	6: 43,642,746 (GRCm39)		probably benign	Het
Trpc2	GTGTCCTA	GTGTCCTATGTCCTA	7: 101,744,420 (GRCm39)		probably null	Het
Tsen54	A	G	11: 115,706,048 (GRCm39)	E90G	probably benign	Het
Ugt1a2	A	T	1: 88,128,968 (GRCm39)	M204L	probably damaging	Het
Vmn2r17	A	T	5: 109,567,972 (GRCm39)	I32F	probably benign	Het
Xkr6	T	C	14: 64,056,833 (GRCm39)	V248A	unknown	Het
Zfhx4	C	A	3: 5,308,067 (GRCm39)	S431Y	probably damaging	Het
Zfp318	T	A	17: 46,723,180 (GRCm39)	S1728T	possibly damaging	Het
Zfp563	T	C	17: 33,308,540 (GRCm39)		probably benign	Het

Other mutations in Or10g3b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00309:Or10g3b	APN	14	52,587,167 (GRCm39)	missense	probably benign	0.00
IGL02485:Or10g3b	APN	14	52,587,501 (GRCm39)	start codon destroyed	possibly damaging	0.89
IGL02828:Or10g3b	APN	14	52,586,799 (GRCm39)	missense	probably benign	0.02
R0744:Or10g3b	UTSW	14	52,586,835 (GRCm39)	missense	probably benign	0.00
R0833:Or10g3b	UTSW	14	52,586,835 (GRCm39)	missense	probably benign	0.00
R2316:Or10g3b	UTSW	14	52,587,395 (GRCm39)	missense	probably benign	0.00
R4898:Or10g3b	UTSW	14	52,586,999 (GRCm39)	missense	probably damaging	1.00
R5018:Or10g3b	UTSW	14	52,586,736 (GRCm39)	missense	possibly damaging	0.81
R5134:Or10g3b	UTSW	14	52,587,248 (GRCm39)	missense	probably benign	0.03
R6819:Or10g3b	UTSW	14	52,587,156 (GRCm39)	missense	probably damaging	1.00
R6877:Or10g3b	UTSW	14	52,587,270 (GRCm39)	missense	possibly damaging	0.95
R7583:Or10g3b	UTSW	14	52,587,360 (GRCm39)	missense	possibly damaging	0.93
R7606:Or10g3b	UTSW	14	52,587,420 (GRCm39)	missense	probably benign	0.33
R7653:Or10g3b	UTSW	14	52,586,889 (GRCm39)	nonsense	probably null
R8111:Or10g3b	UTSW	14	52,587,344 (GRCm39)	missense	possibly damaging	0.70
R8262:Or10g3b	UTSW	14	52,586,625 (GRCm39)	missense	probably damaging	1.00
R8487:Or10g3b	UTSW	14	52,586,696 (GRCm39)	missense	probably damaging	1.00
R8786:Or10g3b	UTSW	14	52,587,021 (GRCm39)	missense	possibly damaging	0.74
R9003:Or10g3b	UTSW	14	52,586,768 (GRCm39)	missense	probably damaging	1.00
R9507:Or10g3b	UTSW	14	52,586,678 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGCTCAGACCTCACTCTTAG -3'
(R):5'- TCCTGCAGTGCTGAAGCTAG -3'

Sequencing Primer
(F):5'- ACCATTCTCCTCAAGGCCATC -3'
(R):5'- CTGAAGCTAGCCTGTGCAGATAC -3'

Posted On

2016-10-05