Mutagenetix > Incidental Mutation

Incidental Mutation 'R5456:Mslnl'

432774

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

043019-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5456 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25736040-25748330 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 25743159 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 177 (D177G)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: D177G

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: D177G

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot12	A	G	13: 91,741,640 (GRCm38)	D37G	probably damaging	Het
Adcy5	T	C	16: 35,298,522 (GRCm38)	F1081S	probably damaging	Het
Apmap	T	A	2: 150,590,069 (GRCm38)	I128L	probably benign	Het
Arhgap12	G	A	18: 6,112,170 (GRCm38)	Q65*	probably null	Het
Baat	C	T	4: 49,502,949 (GRCm38)	V58I	possibly damaging	Het
Bco2	T	C	9: 50,545,344 (GRCm38)		probably null	Het
Bend3	T	C	10: 43,510,546 (GRCm38)	Y312H	probably damaging	Het
Btnl2	A	T	17: 34,363,321 (GRCm38)	Y287F	probably benign	Het
Cd22	T	C	7: 30,876,039 (GRCm38)	I193V	probably benign	Het
Commd3	A	G	2: 18,674,157 (GRCm38)	E95G	probably damaging	Het
Dcbld1	A	G	10: 52,314,390 (GRCm38)	D215G	probably damaging	Het
Elfn1	A	G	5: 139,972,816 (GRCm38)	Y525C	probably damaging	Het
Fam83b	T	C	9: 76,492,595 (GRCm38)	T409A	probably benign	Het
Fshr	T	A	17: 88,986,348 (GRCm38)	I301F	probably benign	Het
Hemgn	C	T	4: 46,396,571 (GRCm38)	V222M	probably damaging	Het
Igsf3	C	T	3: 101,427,221 (GRCm38)	H205Y	probably benign	Het
Mfsd1	T	C	3: 67,589,833 (GRCm38)	I147T	probably benign	Het
Nat2	G	A	8: 67,501,573 (GRCm38)	V112I	probably damaging	Het
Or4c10b	G	A	2: 89,881,258 (GRCm38)	G144E	probably damaging	Het
Or8s5	G	A	15: 98,340,365 (GRCm38)	A208V	probably benign	Het
Pabpc1l	T	C	2: 164,027,660 (GRCm38)	S127P	probably damaging	Het
Poln	A	T	5: 34,007,442 (GRCm38)	L845Q	possibly damaging	Het
Ppm1f	T	A	16: 16,923,746 (GRCm38)	D361E	probably damaging	Het
Rapgef5	T	A	12: 117,728,646 (GRCm38)		probably null	Het
Rarb	G	A	14: 16,436,843 (GRCm38)	T226I	probably damaging	Het
Sel1l3	T	C	5: 53,200,036 (GRCm38)	K205E	probably benign	Het
Sh3glb1	T	A	3: 144,709,353 (GRCm38)	I75L	probably benign	Het
Srgap1	G	A	10: 121,869,811 (GRCm38)	S236L	probably benign	Het
Tmco3	A	G	8: 13,319,815 (GRCm38)	Y609C	probably damaging	Het
Trhde	A	G	10: 114,486,760 (GRCm38)	V712A	possibly damaging	Het
Trim13	A	G	14: 61,605,074 (GRCm38)	D180G	possibly damaging	Het
Tst	T	C	15: 78,399,958 (GRCm38)	E223G	probably damaging	Het
Umodl1	A	G	17: 30,982,289 (GRCm38)	I397M	probably benign	Het
Usp31	T	C	7: 121,670,277 (GRCm38)	D481G	probably damaging	Het
Vps13c	T	A	9: 67,927,447 (GRCm38)	M1686K	possibly damaging	Het
Wdr83	T	C	8: 85,080,208 (GRCm38)	H81R	probably benign	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,743,667 (GRCm38)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,744,775 (GRCm38)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,746,151 (GRCm38)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,747,998 (GRCm38)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,744,103 (GRCm38)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,744,077 (GRCm38)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,743,203 (GRCm38)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,742,965 (GRCm38)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,743,240 (GRCm38)	missense	probably damaging	1.00
R1582:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1629:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,746,181 (GRCm38)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,744,517 (GRCm38)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,744,969 (GRCm38)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,742,934 (GRCm38)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,738,978 (GRCm38)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,738,968 (GRCm38)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,746,165 (GRCm38)	missense	probably benign	0.00
R5645:Mslnl	UTSW	17	25,737,842 (GRCm38)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,746,775 (GRCm38)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,737,902 (GRCm38)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,744,557 (GRCm38)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,746,073 (GRCm38)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,743,212 (GRCm38)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,743,210 (GRCm38)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,736,921 (GRCm38)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,743,183 (GRCm38)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,746,777 (GRCm38)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,746,988 (GRCm38)	missense	probably benign
R8735:Mslnl	UTSW	17	25,745,088 (GRCm38)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,745,073 (GRCm38)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,742,720 (GRCm38)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,742,532 (GRCm38)	intron	probably benign
RF007:Mslnl	UTSW	17	25,743,228 (GRCm38)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- GAACTGCTCACCAACTTGC -3'
(R):5'- TTCAAACCTCAAGCCCCTTG -3'

Sequencing Primer
(F):5'- TTGCCCAACCAGAGAGTTG -3'
(R):5'- AAGCCCCTTGCATCTTATCTGATG -3'

Posted On

2016-10-06