Mutagenetix > Incidental Mutation

Incidental Mutation 'R0479:Hydin'

43283

Institutional Source

Beutler Lab

Gene Symbol

Hydin

Ensembl Gene

ENSMUSG00000059854

Gene Name

HYDIN, axonemal central pair apparatus protein

Synonyms

1700034M11Rik, 4930545D19Rik, hy3, hyrh, hy-3

MMRRC Submission

038679-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.744) question?

Stock #

R0479 (G1)

Quality Score

225

Status

Validated (trace)

Chromosome

Chromosomal Location

110993609-111336885 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 111325720 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Proline at position 4710 (T4710P)

Ref Sequence

ENSEMBL: ENSMUSP00000046204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043141]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000043141
AA Change: T4710P

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000046204
Gene: ENSMUSG00000059854
AA Change: T4710P

Domain	Start	End	E-Value	Type
Pfam:Motile_Sperm	246	325	5.6e-8	PFAM
Pfam:ASH	559	659	9.4e-17	PFAM
low complexity region	788	798	N/A	INTRINSIC
Pfam:PapD-like	848	906	1.2e-6	PFAM
low complexity region	998	1024	N/A	INTRINSIC
low complexity region	1279	1292	N/A	INTRINSIC
internal_repeat_6	1317	1549	5.96e-5	PROSPERO
internal_repeat_5	1355	1502	3.23e-5	PROSPERO
low complexity region	1574	1590	N/A	INTRINSIC
internal_repeat_4	1712	1940	5.14e-6	PROSPERO
coiled coil region	1947	1977	N/A	INTRINSIC
low complexity region	2009	2020	N/A	INTRINSIC
low complexity region	2034	2049	N/A	INTRINSIC
SCOP:d1eq1a_	2305	2403	3e-4	SMART
low complexity region	2404	2419	N/A	INTRINSIC
coiled coil region	2543	2588	N/A	INTRINSIC
low complexity region	2636	2656	N/A	INTRINSIC
internal_repeat_7	2772	3008	8.1e-5	PROSPERO
low complexity region	3660	3670	N/A	INTRINSIC
low complexity region	3919	3934	N/A	INTRINSIC
internal_repeat_5	4046	4190	3.23e-5	PROSPERO
internal_repeat_2	4106	4251	6.03e-7	PROSPERO
internal_repeat_4	4317	4532	5.14e-6	PROSPERO
internal_repeat_3	4403	4689	2.05e-6	PROSPERO
internal_repeat_2	4549	4697	6.03e-7	PROSPERO
low complexity region	4951	4964	N/A	INTRINSIC

Meta Mutation Damage Score

0.1636

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.6%
20x: 93.6%

Validation Efficiency

98% (105/107)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that may be involved in cilia motility. Mutations in this gene cause of autosomal recessive primary ciliary dyskinesia-5, a disorder characterized by the accumulation of cerebrospinal fluid within the ventricles of the brain. A duplicate copy of this gene has been found in humans on chromosome 1. [provided by RefSeq, Jan 2013]
PHENOTYPE: Mice homozygous for a mutation in this gene develop hydrocephaly after birth. Symptoms develop after 3-5 days. Affected animals usually die before 2 months of age. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted(1) Gene trapped(3) Transgenic(1) Spontaneous(2)

Other mutations in this stock

Total: 102 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ackr1	A	G	1: 173,159,712 (GRCm39)	V269A	probably benign	Het
Acsl1	G	A	8: 46,984,109 (GRCm39)	G543R	probably damaging	Het
Adam18	T	C	8: 25,141,838 (GRCm39)	N244D	probably benign	Het
Adgra3	C	T	5: 50,147,607 (GRCm39)	V478M	probably benign	Het
Arhgap39	C	T	15: 76,619,086 (GRCm39)	D833N	probably damaging	Het
Arhgef10	A	T	8: 15,041,070 (GRCm39)	E723V	probably damaging	Het
Arid3a	C	A	10: 79,787,128 (GRCm39)	N519K	possibly damaging	Het
Atrn	T	A	2: 130,841,085 (GRCm39)	Y1162*	probably null	Het
Cacng5	T	A	11: 107,768,777 (GRCm39)	N172Y	probably benign	Het
Cct8	C	T	16: 87,284,594 (GRCm39)	V198M	probably damaging	Het
Cep192	G	A	18: 67,991,089 (GRCm39)	S1857N	probably damaging	Het
Cherp	A	T	8: 73,216,991 (GRCm39)	D657E	possibly damaging	Het
Clca3a2	T	G	3: 144,796,610 (GRCm39)	D199A	probably damaging	Het
Cops7b	A	G	1: 86,532,798 (GRCm39)	T219A	probably benign	Het
Crb1	C	T	1: 139,126,352 (GRCm39)	M1392I	probably damaging	Het
Csf3r	A	C	4: 125,937,616 (GRCm39)	E833D	probably damaging	Het
Cutc	A	G	19: 43,756,655 (GRCm39)	E247G	probably damaging	Het
Cyp2c38	A	G	19: 39,451,449 (GRCm39)	L17P	probably damaging	Het
D5Ertd615e	T	A	5: 45,320,796 (GRCm39)		noncoding transcript	Het
Ddx60	G	T	8: 62,422,691 (GRCm39)	G643W	probably damaging	Het
Depdc1a	C	T	3: 159,226,497 (GRCm39)	T268I	probably damaging	Het
Dgke	T	C	11: 88,943,296 (GRCm39)	E231G	probably benign	Het
Dhrs7b	T	A	11: 60,746,513 (GRCm39)		probably benign	Het
Dll3	A	G	7: 28,000,974 (GRCm39)	V27A	probably damaging	Het
Dnmt3c	T	A	2: 153,556,861 (GRCm39)		probably null	Het
Duox1	T	C	2: 122,176,861 (GRCm39)	F1461L	probably damaging	Het
Enpep	A	G	3: 129,106,323 (GRCm39)	V301A	possibly damaging	Het
Eny2	T	A	15: 44,299,000 (GRCm39)		probably null	Het
Esr1	A	G	10: 4,947,911 (GRCm39)	D488G	probably damaging	Het
Ets1	A	G	9: 32,641,476 (GRCm39)	K110E	probably damaging	Het
Eya2	T	A	2: 165,557,876 (GRCm39)	Y157*	probably null	Het
F830045P16Rik	T	G	2: 129,314,608 (GRCm39)	D223A	possibly damaging	Het
Fbh1	A	G	2: 11,763,230 (GRCm39)	Y475H	probably damaging	Het
Fbxo39	T	C	11: 72,208,419 (GRCm39)	I257T	probably damaging	Het
Fkbp10	T	C	11: 100,306,740 (GRCm39)	V23A	probably damaging	Het
Foxp3	A	G	X: 7,453,583 (GRCm39)	I128V	possibly damaging	Het
Fzd7	T	C	1: 59,522,867 (GRCm39)	F250S	probably damaging	Het
Gaa	A	G	11: 119,172,062 (GRCm39)	T722A	possibly damaging	Het
Gemin5	A	G	11: 58,030,377 (GRCm39)	V816A	probably benign	Het
Glb1l3	T	A	9: 26,740,389 (GRCm39)	T314S	probably benign	Het
H2-Ab1	A	G	17: 34,483,942 (GRCm39)	E101G	possibly damaging	Het
Ica1	C	T	6: 8,754,627 (GRCm39)	V48M	probably damaging	Het
Ica1	T	C	6: 8,754,683 (GRCm39)	Y29C	probably damaging	Het
Ints7	T	A	1: 191,346,666 (GRCm39)		probably null	Het
Iqub	T	C	6: 24,505,809 (GRCm39)	E33G	probably benign	Het
Itgb1bp2	T	A	X: 100,492,806 (GRCm39)	C10S	probably damaging	Het
Katnip	T	C	7: 125,442,518 (GRCm39)	L809S	probably benign	Het
Kcnd1	T	A	X: 7,697,461 (GRCm39)	I391N	possibly damaging	Het
Kdm8	T	C	7: 125,051,812 (GRCm39)	L135P	probably damaging	Het
Ksr1	T	C	11: 78,916,109 (GRCm39)	D574G	probably damaging	Het
Lama5	C	T	2: 179,826,250 (GRCm39)	R2331H	probably benign	Het
Larp1	C	A	11: 57,933,646 (GRCm39)	N357K	possibly damaging	Het
Lgi3	C	T	14: 70,771,992 (GRCm39)		probably benign	Het
Lmbrd1	T	A	1: 24,785,878 (GRCm39)		probably benign	Het
Methig1	A	T	15: 100,272,825 (GRCm39)	K53*	probably null	Het
Mfap3	T	A	11: 57,420,469 (GRCm39)	I150N	probably damaging	Het
Mug1	A	T	6: 121,817,186 (GRCm39)	Q85L	probably benign	Het
Npl	A	G	1: 153,391,155 (GRCm39)	V200A	probably damaging	Het
Nuf2	A	T	1: 169,326,503 (GRCm39)		probably benign	Het
Obscn	A	G	11: 59,003,533 (GRCm39)	V1255A	probably damaging	Het
Or2z8	C	T	8: 72,812,244 (GRCm39)	T240M	probably damaging	Het
Or5h18	C	T	16: 58,847,491 (GRCm39)	V260M	possibly damaging	Het
Or8b51	T	C	9: 38,569,478 (GRCm39)	D70G	probably damaging	Het
P2rx1	T	C	11: 72,903,787 (GRCm39)	V283A	probably damaging	Het
Pex2	C	A	3: 5,626,355 (GRCm39)	L151F	probably damaging	Het
Pias1	A	G	9: 62,800,400 (GRCm39)		probably benign	Het
Pmfbp1	A	T	8: 110,257,105 (GRCm39)		probably benign	Het
Pogz	A	G	3: 94,783,947 (GRCm39)	K545E	possibly damaging	Het
Ppp3cb	T	C	14: 20,553,309 (GRCm39)		probably null	Het
Prl	G	A	13: 27,248,911 (GRCm39)	D189N	probably damaging	Het
Prpf6	A	G	2: 181,292,920 (GRCm39)	N794S	probably benign	Het
Prr36	G	A	8: 4,263,930 (GRCm39)	Q579*	probably null	Het
Ptprq	A	T	10: 107,479,855 (GRCm39)	Y1138*	probably null	Het
Rabepk	A	T	2: 34,675,592 (GRCm39)	H179Q	probably damaging	Het
Rest	T	C	5: 77,430,598 (GRCm39)	S1006P	probably damaging	Het
Rimklb	A	C	6: 122,441,175 (GRCm39)		probably benign	Het
Rnpepl1	T	C	1: 92,846,587 (GRCm39)		probably benign	Het
Ro60	A	T	1: 143,633,489 (GRCm39)	D536E	possibly damaging	Het
Sacs	T	A	14: 61,428,928 (GRCm39)	L329Q	probably damaging	Het
Safb	C	T	17: 56,913,025 (GRCm39)	R914C	probably damaging	Het
Setd5	A	T	6: 113,091,994 (GRCm39)	I272F	probably damaging	Het
Sgk1	G	T	10: 21,872,209 (GRCm39)	A262S	probably benign	Het
Skint2	G	A	4: 112,481,238 (GRCm39)	V34I	possibly damaging	Het
Skint5	G	C	4: 113,512,869 (GRCm39)	Q888E	unknown	Het
Slc4a3	T	C	1: 75,528,472 (GRCm39)		probably benign	Het
Sox10	T	C	15: 79,047,519 (GRCm39)	E133G	probably damaging	Het
Spryd3	G	A	15: 102,038,835 (GRCm39)	R129*	probably null	Het
Stag1	T	A	9: 100,810,144 (GRCm39)	N782K	probably benign	Het
Stam	T	C	2: 14,122,306 (GRCm39)	L132P	probably damaging	Het
Stard9	T	A	2: 120,528,077 (GRCm39)	S1445T	probably damaging	Het
Syt5	C	T	7: 4,546,108 (GRCm39)	R94Q	probably benign	Het
Tbc1d23	A	T	16: 56,992,177 (GRCm39)	H594Q	probably damaging	Het
Tecta	T	A	9: 42,249,235 (GRCm39)	I1871F	probably damaging	Het
Tek	A	G	4: 94,692,549 (GRCm39)	D219G	probably benign	Het
Thrb	T	C	14: 18,033,643 (GRCm38)	F469L	probably damaging	Het
Tyr	T	C	7: 87,142,429 (GRCm39)	S44G	possibly damaging	Het
Usp20	T	A	2: 30,907,487 (GRCm39)	V673E	probably benign	Het
Usp28	T	C	9: 48,948,513 (GRCm39)	S873P	probably damaging	Het
Usp43	C	T	11: 67,788,100 (GRCm39)	V306M	possibly damaging	Het
Wdr17	G	T	8: 55,104,456 (GRCm39)		probably null	Het
Wsb2	T	G	5: 117,514,744 (GRCm39)		probably benign	Het
Xkrx	A	T	X: 133,051,715 (GRCm39)	L312Q	probably damaging	Het

Other mutations in Hydin

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00338:Hydin	APN	8	111,296,434 (GRCm39)	missense	possibly damaging	0.69
IGL00432:Hydin	APN	8	111,327,884 (GRCm39)	missense	probably damaging	0.98
IGL01025:Hydin	APN	8	111,053,033 (GRCm39)	missense	probably benign	0.38
IGL01140:Hydin	APN	8	111,124,694 (GRCm39)	missense	probably benign	0.14
IGL01317:Hydin	APN	8	111,053,078 (GRCm39)	missense	probably damaging	0.98
IGL01473:Hydin	APN	8	111,038,792 (GRCm39)	missense	probably benign	0.08
IGL01473:Hydin	APN	8	111,081,585 (GRCm39)	missense	probably damaging	1.00
IGL01610:Hydin	APN	8	111,284,345 (GRCm39)	missense	probably benign	0.00
IGL01685:Hydin	APN	8	111,081,665 (GRCm39)	nonsense	probably null
IGL01734:Hydin	APN	8	111,217,421 (GRCm39)	nonsense	probably null
IGL01743:Hydin	APN	8	111,319,408 (GRCm39)	missense	possibly damaging	0.94
IGL01829:Hydin	APN	8	111,316,154 (GRCm39)	missense	possibly damaging	0.68
IGL01919:Hydin	APN	8	111,245,806 (GRCm39)	missense	possibly damaging	0.89
IGL01946:Hydin	APN	8	111,217,350 (GRCm39)	missense	possibly damaging	0.91
IGL01983:Hydin	APN	8	111,241,527 (GRCm39)	missense	probably benign	0.02
IGL02122:Hydin	APN	8	111,221,047 (GRCm39)	missense	possibly damaging	0.86
IGL02140:Hydin	APN	8	111,293,570 (GRCm39)	missense	probably benign
IGL02158:Hydin	APN	8	111,336,598 (GRCm39)	missense	possibly damaging	0.89
IGL02167:Hydin	APN	8	111,145,055 (GRCm39)	missense	possibly damaging	0.96
IGL02171:Hydin	APN	8	111,178,590 (GRCm39)	nonsense	probably null
IGL02185:Hydin	APN	8	111,233,108 (GRCm39)	missense	possibly damaging	0.86
IGL02517:Hydin	APN	8	111,293,604 (GRCm39)	missense	probably benign	0.01
IGL02639:Hydin	APN	8	111,265,081 (GRCm39)	missense	probably benign	0.01
IGL02644:Hydin	APN	8	111,265,100 (GRCm39)	missense	probably damaging	1.00
IGL02652:Hydin	APN	8	111,316,154 (GRCm39)	missense	possibly damaging	0.68
IGL02658:Hydin	APN	8	111,139,908 (GRCm39)	missense	possibly damaging	0.86
IGL02706:Hydin	APN	8	111,137,198 (GRCm39)	missense	probably damaging	0.99
IGL02892:Hydin	APN	8	111,325,591 (GRCm39)	missense	possibly damaging	0.89
IGL02947:Hydin	APN	8	111,145,094 (GRCm39)	missense	probably damaging	0.96
IGL03136:Hydin	APN	8	111,145,156 (GRCm39)	missense	probably benign	0.22
IGL03248:Hydin	APN	8	111,321,921 (GRCm39)	missense	probably damaging	0.97
IGL03251:Hydin	APN	8	111,217,228 (GRCm39)	missense	probably damaging	1.00
IGL03350:Hydin	APN	8	111,038,856 (GRCm39)	missense	possibly damaging	0.86
IGL03366:Hydin	APN	8	110,993,995 (GRCm39)	missense	unknown
IGL03404:Hydin	APN	8	111,296,409 (GRCm39)	missense	probably benign	0.06
Franz_joseph	UTSW	8	111,327,950 (GRCm39)	missense	probably damaging	1.00
jahreszeiten	UTSW	8	111,295,991 (GRCm39)	missense	probably damaging	1.00
maria	UTSW	8	111,235,759 (GRCm39)	splice site	probably benign
schoepfung	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
surprise	UTSW	8	111,254,648 (GRCm39)	missense	probably benign
teresa	UTSW	8	111,336,303 (GRCm39)	missense	possibly damaging	0.79
BB001:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
BB004:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
BB011:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
BB014:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
P0005:Hydin	UTSW	8	111,220,921 (GRCm39)	critical splice acceptor site	probably null
R0099:Hydin	UTSW	8	111,316,193 (GRCm39)	missense	probably damaging	1.00
R0125:Hydin	UTSW	8	111,189,163 (GRCm39)	missense	probably benign	0.12
R0157:Hydin	UTSW	8	111,026,642 (GRCm39)	missense	possibly damaging	0.86
R0241:Hydin	UTSW	8	111,124,655 (GRCm39)	missense	probably benign	0.04
R0241:Hydin	UTSW	8	111,124,655 (GRCm39)	missense	probably benign	0.04
R0255:Hydin	UTSW	8	111,291,650 (GRCm39)	missense	probably benign	0.00
R0352:Hydin	UTSW	8	111,296,533 (GRCm39)	critical splice donor site	probably null
R0379:Hydin	UTSW	8	111,235,759 (GRCm39)	splice site	probably benign
R0468:Hydin	UTSW	8	111,139,855 (GRCm39)	missense	possibly damaging	0.96
R0477:Hydin	UTSW	8	111,145,130 (GRCm39)	missense	probably damaging	1.00
R0539:Hydin	UTSW	8	111,249,704 (GRCm39)	missense	probably benign
R0550:Hydin	UTSW	8	111,314,407 (GRCm39)	missense	probably benign	0.01
R0571:Hydin	UTSW	8	111,240,735 (GRCm39)	splice site	probably null
R0606:Hydin	UTSW	8	111,276,430 (GRCm39)	splice site	probably benign
R0789:Hydin	UTSW	8	111,293,603 (GRCm39)	missense	possibly damaging	0.53
R0849:Hydin	UTSW	8	111,325,616 (GRCm39)	missense	probably damaging	1.00
R0946:Hydin	UTSW	8	111,257,685 (GRCm39)	missense	probably benign	0.25
R1201:Hydin	UTSW	8	111,296,487 (GRCm39)	missense	probably benign	0.01
R1375:Hydin	UTSW	8	111,232,854 (GRCm39)	critical splice donor site	probably null
R1385:Hydin	UTSW	8	111,249,836 (GRCm39)	missense	probably benign	0.40
R1411:Hydin	UTSW	8	111,301,663 (GRCm39)	missense	probably benign	0.04
R1437:Hydin	UTSW	8	111,308,617 (GRCm39)	nonsense	probably null
R1447:Hydin	UTSW	8	111,249,798 (GRCm39)	missense	probably damaging	1.00
R1448:Hydin	UTSW	8	111,173,217 (GRCm39)	missense	probably benign	0.27
R1466:Hydin	UTSW	8	111,259,585 (GRCm39)	missense	possibly damaging	0.47
R1466:Hydin	UTSW	8	111,259,585 (GRCm39)	missense	possibly damaging	0.47
R1523:Hydin	UTSW	8	111,259,903 (GRCm39)	missense	probably benign	0.05
R1544:Hydin	UTSW	8	111,301,486 (GRCm39)	missense	probably benign	0.30
R1581:Hydin	UTSW	8	111,137,092 (GRCm39)	missense	probably benign
R1584:Hydin	UTSW	8	111,307,447 (GRCm39)	missense	probably benign	0.27
R1598:Hydin	UTSW	8	111,137,306 (GRCm39)	missense	possibly damaging	0.96
R1633:Hydin	UTSW	8	111,233,614 (GRCm39)	missense	probably benign	0.10
R1777:Hydin	UTSW	8	111,316,203 (GRCm39)	missense	probably benign	0.14
R1817:Hydin	UTSW	8	111,259,459 (GRCm39)	missense	probably benign	0.00
R1828:Hydin	UTSW	8	111,237,526 (GRCm39)	missense	probably benign	0.03
R1837:Hydin	UTSW	8	111,296,257 (GRCm39)	missense	probably benign	0.20
R1848:Hydin	UTSW	8	111,296,440 (GRCm39)	missense	probably benign	0.19
R1869:Hydin	UTSW	8	111,227,337 (GRCm39)	missense	possibly damaging	0.94
R1909:Hydin	UTSW	8	111,314,404 (GRCm39)	missense	probably damaging	1.00
R1928:Hydin	UTSW	8	111,229,579 (GRCm39)	missense	possibly damaging	0.93
R1950:Hydin	UTSW	8	111,336,619 (GRCm39)	missense	possibly damaging	0.64
R2095:Hydin	UTSW	8	111,189,289 (GRCm39)	missense	probably damaging	0.96
R2172:Hydin	UTSW	8	111,308,681 (GRCm39)	missense	probably benign	0.42
R2217:Hydin	UTSW	8	111,145,138 (GRCm39)	missense	probably benign
R2248:Hydin	UTSW	8	111,304,835 (GRCm39)	missense	probably benign	0.09
R2272:Hydin	UTSW	8	111,035,764 (GRCm39)	missense	probably benign	0.01
R2294:Hydin	UTSW	8	111,026,591 (GRCm39)	missense	probably damaging	0.99
R2315:Hydin	UTSW	8	111,124,676 (GRCm39)	missense	probably benign	0.01
R2330:Hydin	UTSW	8	111,291,641 (GRCm39)	missense	probably benign	0.01
R2374:Hydin	UTSW	8	111,291,780 (GRCm39)	missense	probably damaging	1.00
R2446:Hydin	UTSW	8	111,314,347 (GRCm39)	missense	possibly damaging	0.82
R2484:Hydin	UTSW	8	111,239,747 (GRCm39)	missense	possibly damaging	0.76
R2698:Hydin	UTSW	8	111,336,561 (GRCm39)	missense	possibly damaging	0.70
R2843:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2844:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2846:Hydin	UTSW	8	111,245,746 (GRCm39)	missense	probably benign
R2882:Hydin	UTSW	8	111,293,555 (GRCm39)	missense	possibly damaging	0.92
R2937:Hydin	UTSW	8	111,130,927 (GRCm39)	missense	possibly damaging	0.88
R3031:Hydin	UTSW	8	111,329,848 (GRCm39)	missense	possibly damaging	0.83
R3038:Hydin	UTSW	8	111,309,321 (GRCm39)	missense	probably damaging	1.00
R3121:Hydin	UTSW	8	111,233,138 (GRCm39)	missense	probably benign
R3157:Hydin	UTSW	8	110,994,005 (GRCm39)	missense	unknown
R3547:Hydin	UTSW	8	111,308,699 (GRCm39)	missense	possibly damaging	0.85
R3696:Hydin	UTSW	8	111,329,911 (GRCm39)	missense	probably damaging	1.00
R3850:Hydin	UTSW	8	111,290,561 (GRCm39)	missense	probably damaging	0.99
R3896:Hydin	UTSW	8	111,235,711 (GRCm39)	missense	possibly damaging	0.93
R3983:Hydin	UTSW	8	111,118,957 (GRCm39)	missense	probably damaging	1.00
R4031:Hydin	UTSW	8	111,336,679 (GRCm39)	missense	probably benign	0.30
R4072:Hydin	UTSW	8	111,231,888 (GRCm39)	missense	possibly damaging	0.68
R4095:Hydin	UTSW	8	111,268,179 (GRCm39)	missense	probably damaging	0.98
R4176:Hydin	UTSW	8	111,320,452 (GRCm39)	missense	probably benign	0.00
R4213:Hydin	UTSW	8	111,183,139 (GRCm39)	missense	possibly damaging	0.91
R4412:Hydin	UTSW	8	111,142,368 (GRCm39)	missense	probably damaging	0.99
R4471:Hydin	UTSW	8	111,313,764 (GRCm39)	missense	probably damaging	1.00
R4474:Hydin	UTSW	8	111,290,497 (GRCm39)	missense	probably benign	0.11
R4495:Hydin	UTSW	8	111,322,034 (GRCm39)	missense	probably damaging	0.99
R4508:Hydin	UTSW	8	111,245,886 (GRCm39)	missense	possibly damaging	0.91
R4578:Hydin	UTSW	8	110,993,971 (GRCm39)	missense	unknown
R4583:Hydin	UTSW	8	111,321,857 (GRCm39)	missense	probably benign	0.36
R4600:Hydin	UTSW	8	111,293,582 (GRCm39)	missense	probably benign	0.04
R4681:Hydin	UTSW	8	111,233,103 (GRCm39)	missense	possibly damaging	0.85
R4685:Hydin	UTSW	8	111,189,154 (GRCm39)	missense	probably damaging	0.99
R4689:Hydin	UTSW	8	111,322,046 (GRCm39)	missense	probably benign	0.18
R4735:Hydin	UTSW	8	111,282,264 (GRCm39)	critical splice donor site	probably null
R4736:Hydin	UTSW	8	111,249,840 (GRCm39)	missense	probably benign	0.02
R4740:Hydin	UTSW	8	111,173,071 (GRCm39)	missense	probably benign	0.06
R4771:Hydin	UTSW	8	111,259,515 (GRCm39)	missense	probably benign
R4777:Hydin	UTSW	8	111,137,096 (GRCm39)	missense	probably damaging	0.98
R4859:Hydin	UTSW	8	111,233,126 (GRCm39)	missense	possibly damaging	0.93
R4911:Hydin	UTSW	8	111,322,070 (GRCm39)	missense	probably benign	0.01
R4964:Hydin	UTSW	8	111,217,305 (GRCm39)	missense	possibly damaging	0.86
R4965:Hydin	UTSW	8	111,124,727 (GRCm39)	missense	probably benign
R4989:Hydin	UTSW	8	111,290,554 (GRCm39)	missense	possibly damaging	0.84
R4995:Hydin	UTSW	8	111,296,274 (GRCm39)	missense	probably damaging	0.97
R5059:Hydin	UTSW	8	111,232,401 (GRCm39)	missense	probably damaging	0.96
R5071:Hydin	UTSW	8	111,265,105 (GRCm39)	missense	probably benign	0.03
R5073:Hydin	UTSW	8	111,265,105 (GRCm39)	missense	probably benign	0.03
R5092:Hydin	UTSW	8	111,309,300 (GRCm39)	missense	probably benign	0.16
R5156:Hydin	UTSW	8	111,336,333 (GRCm39)	missense	probably benign	0.00
R5166:Hydin	UTSW	8	111,249,774 (GRCm39)	missense	possibly damaging	0.89
R5189:Hydin	UTSW	8	111,139,843 (GRCm39)	critical splice acceptor site	probably null
R5243:Hydin	UTSW	8	111,232,380 (GRCm39)	missense	possibly damaging	0.92
R5244:Hydin	UTSW	8	111,259,451 (GRCm39)	missense	possibly damaging	0.77
R5256:Hydin	UTSW	8	111,313,855 (GRCm39)	missense	possibly damaging	0.92
R5266:Hydin	UTSW	8	111,061,416 (GRCm39)	missense	possibly damaging	0.87
R5283:Hydin	UTSW	8	111,178,612 (GRCm39)	missense	possibly damaging	0.96
R5343:Hydin	UTSW	8	111,212,051 (GRCm39)	missense	probably benign	0.40
R5359:Hydin	UTSW	8	111,265,004 (GRCm39)	missense	probably benign	0.00
R5390:Hydin	UTSW	8	111,322,099 (GRCm39)	missense	probably benign
R5394:Hydin	UTSW	8	111,266,474 (GRCm39)	splice site	probably null
R5441:Hydin	UTSW	8	111,291,741 (GRCm39)	missense	possibly damaging	0.72
R5461:Hydin	UTSW	8	111,245,863 (GRCm39)	missense	probably damaging	0.96
R5662:Hydin	UTSW	8	111,307,341 (GRCm39)	missense	probably benign	0.02
R5695:Hydin	UTSW	8	111,261,915 (GRCm39)	missense	probably benign	0.35
R5732:Hydin	UTSW	8	111,178,690 (GRCm39)	missense	probably benign	0.03
R5774:Hydin	UTSW	8	111,298,547 (GRCm39)	nonsense	probably null
R5780:Hydin	UTSW	8	111,312,712 (GRCm39)	missense	probably damaging	1.00
R5787:Hydin	UTSW	8	111,052,985 (GRCm39)	missense	probably damaging	0.99
R5802:Hydin	UTSW	8	111,178,692 (GRCm39)	missense	possibly damaging	0.86
R5841:Hydin	UTSW	8	111,259,846 (GRCm39)	missense	possibly damaging	0.76
R5856:Hydin	UTSW	8	111,268,474 (GRCm39)	missense	probably damaging	0.99
R5893:Hydin	UTSW	8	111,217,308 (GRCm39)	missense	probably benign	0.12
R5963:Hydin	UTSW	8	111,220,926 (GRCm39)	missense	possibly damaging	0.93
R6008:Hydin	UTSW	8	111,325,717 (GRCm39)	missense	probably benign	0.02
R6019:Hydin	UTSW	8	111,293,252 (GRCm39)	missense	probably benign
R6038:Hydin	UTSW	8	111,325,663 (GRCm39)	missense	probably benign	0.16
R6038:Hydin	UTSW	8	111,325,663 (GRCm39)	missense	probably benign	0.16
R6133:Hydin	UTSW	8	111,327,908 (GRCm39)	missense	probably benign	0.00
R6135:Hydin	UTSW	8	111,189,292 (GRCm39)	missense	possibly damaging	0.85
R6157:Hydin	UTSW	8	111,254,648 (GRCm39)	missense	probably benign
R6209:Hydin	UTSW	8	111,320,434 (GRCm39)	missense	probably benign	0.05
R6238:Hydin	UTSW	8	111,118,743 (GRCm39)	splice site	probably null
R6293:Hydin	UTSW	8	111,324,543 (GRCm39)	missense	possibly damaging	0.83
R6340:Hydin	UTSW	8	111,081,574 (GRCm39)	splice site	probably null
R6349:Hydin	UTSW	8	111,145,091 (GRCm39)	nonsense	probably null
R6357:Hydin	UTSW	8	111,268,289 (GRCm39)	missense	possibly damaging	0.86
R6385:Hydin	UTSW	8	111,038,856 (GRCm39)	missense	possibly damaging	0.86
R6396:Hydin	UTSW	8	111,233,521 (GRCm39)	missense	probably damaging	0.96
R6466:Hydin	UTSW	8	111,233,600 (GRCm39)	missense	possibly damaging	0.85
R6648:Hydin	UTSW	8	111,252,299 (GRCm39)	splice site	probably null
R6671:Hydin	UTSW	8	111,327,950 (GRCm39)	missense	probably damaging	1.00
R6695:Hydin	UTSW	8	111,053,092 (GRCm39)	missense	probably benign	0.05
R6800:Hydin	UTSW	8	111,324,603 (GRCm39)	missense	probably benign	0.09
R6841:Hydin	UTSW	8	111,265,007 (GRCm39)	missense	probably benign	0.09
R6867:Hydin	UTSW	8	111,266,434 (GRCm39)	missense	probably benign	0.08
R6889:Hydin	UTSW	8	111,259,488 (GRCm39)	missense	possibly damaging	0.79
R6895:Hydin	UTSW	8	111,038,883 (GRCm39)	missense	probably benign	0.00
R6940:Hydin	UTSW	8	111,217,243 (GRCm39)	missense	probably damaging	1.00
R6951:Hydin	UTSW	8	111,124,757 (GRCm39)	missense	probably benign
R6980:Hydin	UTSW	8	111,139,916 (GRCm39)	missense	possibly damaging	0.91
R6981:Hydin	UTSW	8	111,257,704 (GRCm39)	missense	possibly damaging	0.89
R7061:Hydin	UTSW	8	111,329,920 (GRCm39)	missense	possibly damaging	0.90
R7085:Hydin	UTSW	8	111,329,962 (GRCm39)	missense	probably benign	0.03
R7086:Hydin	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
R7110:Hydin	UTSW	8	111,081,583 (GRCm39)	critical splice acceptor site	probably null
R7158:Hydin	UTSW	8	111,336,303 (GRCm39)	missense	possibly damaging	0.79
R7163:Hydin	UTSW	8	111,329,968 (GRCm39)	missense	probably benign	0.25
R7209:Hydin	UTSW	8	111,216,424 (GRCm39)	nonsense	probably null
R7244:Hydin	UTSW	8	111,276,307 (GRCm39)	missense	probably damaging	0.98
R7347:Hydin	UTSW	8	111,326,994 (GRCm39)	missense	probably benign	0.06
R7349:Hydin	UTSW	8	111,124,803 (GRCm39)	splice site	probably null
R7359:Hydin	UTSW	8	111,232,733 (GRCm39)	missense	probably damaging	0.98
R7365:Hydin	UTSW	8	111,327,905 (GRCm39)	missense	probably damaging	1.00
R7365:Hydin	UTSW	8	111,284,294 (GRCm39)	missense	probably damaging	0.99
R7436:Hydin	UTSW	8	111,310,546 (GRCm39)	missense	probably damaging	0.96
R7528:Hydin	UTSW	8	111,107,204 (GRCm39)	nonsense	probably null
R7544:Hydin	UTSW	8	111,316,157 (GRCm39)	missense	probably benign	0.35
R7625:Hydin	UTSW	8	111,268,476 (GRCm39)	missense	probably benign	0.01
R7713:Hydin	UTSW	8	111,320,444 (GRCm39)	missense	possibly damaging	0.69
R7763:Hydin	UTSW	8	111,232,475 (GRCm39)	missense	possibly damaging	0.92
R7771:Hydin	UTSW	8	111,291,717 (GRCm39)	missense	probably damaging	0.97
R7794:Hydin	UTSW	8	111,235,715 (GRCm39)	missense	probably damaging	1.00
R7833:Hydin	UTSW	8	111,316,092 (GRCm39)	missense	probably damaging	0.99
R7894:Hydin	UTSW	8	111,239,642 (GRCm39)	missense	possibly damaging	0.88
R7899:Hydin	UTSW	8	111,314,380 (GRCm39)	missense	probably benign	0.00
R7908:Hydin	UTSW	8	111,237,499 (GRCm39)	missense	probably benign	0.01
R7912:Hydin	UTSW	8	111,282,239 (GRCm39)	missense	possibly damaging	0.68
R7919:Hydin	UTSW	8	110,993,971 (GRCm39)	missense	unknown
R7924:Hydin	UTSW	8	111,145,103 (GRCm39)	missense	possibly damaging	0.93
R7927:Hydin	UTSW	8	111,307,476 (GRCm39)	missense	possibly damaging	0.89
R7970:Hydin	UTSW	8	111,035,723 (GRCm39)	missense	probably damaging	0.99
R7993:Hydin	UTSW	8	111,306,264 (GRCm39)	missense	probably benign
R8011:Hydin	UTSW	8	111,310,541 (GRCm39)	missense	probably damaging	1.00
R8041:Hydin	UTSW	8	111,301,626 (GRCm39)	missense	probably benign	0.02
R8080:Hydin	UTSW	8	111,261,863 (GRCm39)	missense	probably benign	0.32
R8081:Hydin	UTSW	8	111,092,101 (GRCm39)	missense	possibly damaging	0.93
R8095:Hydin	UTSW	8	111,295,991 (GRCm39)	missense	probably damaging	1.00
R8157:Hydin	UTSW	8	111,178,668 (GRCm39)	missense	probably benign	0.33
R8186:Hydin	UTSW	8	111,336,277 (GRCm39)	missense	probably benign	0.14
R8205:Hydin	UTSW	8	111,319,270 (GRCm39)	missense	possibly damaging	0.57
R8263:Hydin	UTSW	8	111,178,705 (GRCm39)	missense	probably benign	0.00
R8288:Hydin	UTSW	8	111,233,661 (GRCm39)	missense	probably damaging	0.96
R8298:Hydin	UTSW	8	111,327,015 (GRCm39)	missense	probably damaging	1.00
R8309:Hydin	UTSW	8	111,334,534 (GRCm39)	missense	probably benign	0.18
R8348:Hydin	UTSW	8	111,329,878 (GRCm39)	missense	possibly damaging	0.68
R8356:Hydin	UTSW	8	111,259,756 (GRCm39)	missense	possibly damaging	0.67
R8406:Hydin	UTSW	8	111,336,543 (GRCm39)	missense	possibly damaging	0.94
R8415:Hydin	UTSW	8	111,178,626 (GRCm39)	missense	probably damaging	1.00
R8417:Hydin	UTSW	8	111,296,024 (GRCm39)	missense	probably benign	0.28
R8432:Hydin	UTSW	8	111,324,583 (GRCm39)	missense	probably benign	0.02
R8437:Hydin	UTSW	8	111,189,367 (GRCm39)	missense	probably damaging	0.96
R8463:Hydin	UTSW	8	111,237,553 (GRCm39)	missense	probably benign	0.22
R8508:Hydin	UTSW	8	111,308,650 (GRCm39)	missense	probably benign	0.00
R8510:Hydin	UTSW	8	111,233,202 (GRCm39)	missense	probably damaging	1.00
R8560:Hydin	UTSW	8	111,265,106 (GRCm39)	missense	probably benign	0.09
R8682:Hydin	UTSW	8	111,035,798 (GRCm39)	missense	probably damaging	0.96
R8697:Hydin	UTSW	8	111,259,515 (GRCm39)	missense	probably benign
R8857:Hydin	UTSW	8	111,298,587 (GRCm39)	critical splice donor site	probably null
R8866:Hydin	UTSW	8	111,308,779 (GRCm39)	missense	possibly damaging	0.89
R8878:Hydin	UTSW	8	111,035,720 (GRCm39)	missense	probably benign	0.12
R8897:Hydin	UTSW	8	111,316,112 (GRCm39)	missense	probably benign
R8987:Hydin	UTSW	8	111,239,766 (GRCm39)	nonsense	probably null
R9072:Hydin	UTSW	8	110,994,083 (GRCm39)	critical splice donor site	probably null
R9073:Hydin	UTSW	8	110,994,083 (GRCm39)	critical splice donor site	probably null
R9102:Hydin	UTSW	8	111,235,546 (GRCm39)	missense	probably benign	0.33
R9224:Hydin	UTSW	8	111,259,516 (GRCm39)	missense	probably benign
R9255:Hydin	UTSW	8	111,261,972 (GRCm39)	missense	probably benign	0.23
R9257:Hydin	UTSW	8	111,301,648 (GRCm39)	missense	probably damaging	0.99
R9261:Hydin	UTSW	8	110,994,047 (GRCm39)	missense	unknown
R9273:Hydin	UTSW	8	111,233,580 (GRCm39)	missense	probably damaging	0.98
R9376:Hydin	UTSW	8	111,124,695 (GRCm39)	missense	possibly damaging	0.70
R9380:Hydin	UTSW	8	111,290,504 (GRCm39)	missense	probably benign	0.07
R9386:Hydin	UTSW	8	111,314,362 (GRCm39)	missense	probably benign
R9406:Hydin	UTSW	8	111,314,412 (GRCm39)	missense	probably null	0.96
R9492:Hydin	UTSW	8	111,326,877 (GRCm39)	missense	possibly damaging	0.68
R9513:Hydin	UTSW	8	111,322,114 (GRCm39)	missense	probably damaging	0.99
R9562:Hydin	UTSW	8	111,312,786 (GRCm39)	missense	probably benign	0.11
R9664:Hydin	UTSW	8	111,220,965 (GRCm39)	missense	probably benign	0.01
R9733:Hydin	UTSW	8	111,262,011 (GRCm39)	missense	probably benign
R9753:Hydin	UTSW	8	111,217,398 (GRCm39)	missense	possibly damaging	0.85
X0063:Hydin	UTSW	8	111,277,951 (GRCm39)	missense	probably damaging	1.00
Z1088:Hydin	UTSW	8	111,319,423 (GRCm39)	frame shift	probably null
Z1088:Hydin	UTSW	8	111,312,680 (GRCm39)	missense	probably benign	0.00
Z1088:Hydin	UTSW	8	111,026,605 (GRCm39)	missense	probably benign	0.12
Z1176:Hydin	UTSW	8	111,268,232 (GRCm39)	missense	possibly damaging	0.65
Z1177:Hydin	UTSW	8	111,313,774 (GRCm39)	frame shift	probably null
Z1177:Hydin	UTSW	8	111,176,864 (GRCm39)	missense	possibly damaging	0.47
Z1177:Hydin	UTSW	8	111,107,242 (GRCm39)	missense	probably damaging	1.00
Z1177:Hydin	UTSW	8	111,336,621 (GRCm39)	missense	probably benign	0.10
Z1188:Hydin	UTSW	8	111,142,419 (GRCm39)	missense	probably benign	0.40

Predicted Primers

PCR Primer
(F):5'- GAGGTGATTTTCCTGCTTCCTCCAG -3'
(R):5'- ACTGCCTGCCAACTGTTATGGTTC -3'

Sequencing Primer
(F):5'- CTCCAGGTGGTGAATTTCAAC -3'
(R):5'- GCCAACTGTTATGGTTCCCTATC -3'

Posted On

2013-05-23