Mutagenetix > Incidental Mutation

Incidental Mutation 'R5461:Vmn2r104'

433049

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

043023-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R5461 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 20030081 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 643 (I643F)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168050
AA Change: I643F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: I643F

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930522L14Rik	A	T	5: 109,736,777	I405K	possibly damaging	Het
4930562C15Rik	G	A	16: 4,864,363	G180E	probably damaging	Het
Adgrg6	A	T	10: 14,420,504	W1079R	probably damaging	Het
Arsa	T	C	15: 89,473,275	H495R	probably benign	Het
Bptf	T	C	11: 107,061,764	T2088A	probably damaging	Het
Brsk2	T	C	7: 141,987,906	L152P	probably damaging	Het
C8a	T	C	4: 104,815,845		probably benign	Het
Ccdc177	G	A	12: 80,758,042	A486V	unknown	Het
Cpa2	A	G	6: 30,544,181	T38A	probably benign	Het
Crim1	T	G	17: 78,237,807	C133G	probably damaging	Het
Dnah2	C	T	11: 69,473,351		probably null	Het
Ep400	G	A	5: 110,676,684	Q2392*	probably null	Het
Exoc2	C	T	13: 30,925,755	S210N	possibly damaging	Het
Gm7003	A	G	12: 113,803,227		probably benign	Het
Hydin	A	G	8: 110,519,231	K2192R	probably damaging	Het
Ica1l	T	C	1: 60,013,851	D176G	probably damaging	Het
Ints10	G	A	8: 68,794,041	E8K	possibly damaging	Het
Itgb8	T	C	12: 119,168,005	E635G	probably benign	Het
Kmt2d	G	A	15: 98,852,109		probably benign	Het
Kng1	A	G	16: 23,079,137	H429R	probably benign	Het
Mcm3	T	C	1: 20,814,437	I281V	probably benign	Het
Msi1	T	C	5: 115,441,391	S200P	possibly damaging	Het
Nat3	T	C	8: 67,547,862	L131P	probably damaging	Het
Ncor2	T	C	5: 125,027,113	E1752G	probably damaging	Het
Nnt	C	T	13: 119,368,595	A414T	possibly damaging	Het
Nrp2	C	T	1: 62,747,211	Q292*	probably null	Het
Olfr552	G	A	7: 102,604,408	G18D	probably damaging	Het
Olfr615	T	A	7: 103,560,573	L32Q	probably damaging	Het
Otop1	A	T	5: 38,299,715	I273F	probably damaging	Het
Phactr3	T	A	2: 178,278,901	N177K	probably benign	Het
Pik3c2b	C	T	1: 133,099,702	T1313I	possibly damaging	Het
Pikfyve	C	A	1: 65,235,033	D677E	probably damaging	Het
Poc1a	T	C	9: 106,288,010	F157L	probably damaging	Het
Prodh2	G	T	7: 30,494,523	R185L	possibly damaging	Het
Rcc1	T	C	4: 132,334,186	I350M	probably benign	Het
Rhd	G	A	4: 134,884,617	A249T	probably damaging	Het
Rtf2	A	G	2: 172,445,332	Y57C	probably damaging	Het
Shmt1	A	G	11: 60,794,899	S284P	possibly damaging	Het
Shpk	T	C	11: 73,199,535	V6A	probably benign	Het
Slc4a5	T	C	6: 83,285,854	V661A	probably benign	Het
Spesp1	A	T	9: 62,272,732	L298Q	probably damaging	Het
Tas1r2	C	A	4: 139,660,009	Q231K	probably benign	Het
Tcp11l1	T	C	2: 104,688,511	Y280C	probably benign	Het
Tnip1	T	C	11: 54,910,799		probably benign	Het
Topbp1	T	A	9: 103,315,196	D295E	probably benign	Het
Unc79	C	T	12: 103,112,138	L1521F	probably damaging	Het
Usp10	T	C	8: 119,956,667	I759T	probably benign	Het
Vmn1r30	A	T	6: 58,435,774	C24*	probably null	Het
Wwc1	T	A	11: 35,867,372	T716S	probably damaging	Het
Zfp949	C	T	9: 88,569,484	T369M	probably benign	Het
Zhx3	A	G	2: 160,780,018	V743A	probably benign	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20042725	missense	probably benign
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20029475	missense	probably benign	0.01
R7514:Vmn2r104	UTSW	17	20029529	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8900:Vmn2r104	UTSW	17	20041662	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGAAGCTGGATCAGTGTGC -3'
(R):5'- CTTTCTGGCCTATGAAGACCCC -3'

Sequencing Primer
(F):5'- AGGAATGATGTATCTTGGACCCC -3'
(R):5'- CCTTGGGGATGGCTCTAGC -3'

Posted On

2016-10-06