Mutagenetix > Incidental Mutation

Incidental Mutation 'R5528:Vmn2r23'

433526

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r23

Ensembl Gene

ENSMUSG00000091620

Gene Name

vomeronasal 2, receptor 23

Synonyms

EG435916

MMRRC Submission

043086-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R5528 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

123679780-123719198 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123689961 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 279 (D279G)

Ref Sequence

ENSEMBL: ENSMUSP00000126682 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172391]

AlphaFold

E9PXI5

Predicted Effect

probably damaging
Transcript: ENSMUST00000172391
AA Change: D279G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126682
Gene: ENSMUSG00000091620
AA Change: D279G

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	79	461	1.7e-31	PFAM
Pfam:NCD3G	513	566	1.2e-23	PFAM
Pfam:7tm_3	596	834	1.5e-55	PFAM

Coding Region Coverage

1x: 98.6%
3x: 97.4%
10x: 95.4%
20x: 91.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	T	C	3: 137,772,260 (GRCm39)	L483P	probably benign	Het
4930519G04Rik	T	G	5: 115,012,415 (GRCm39)		probably null	Het
Ablim2	C	T	5: 36,013,510 (GRCm39)	Q148*	probably null	Het
Ap3s2	A	T	7: 79,530,234 (GRCm39)	*194R	probably null	Het
Arpp21	C	T	9: 111,978,421 (GRCm39)	A235T	probably benign	Het
C9orf72	T	C	4: 35,213,556 (GRCm39)	D198G	probably benign	Het
Ccdc33	T	C	9: 57,936,078 (GRCm39)	D939G	probably benign	Het
Celsr2	A	T	3: 108,320,610 (GRCm39)	I734N	probably damaging	Het
Clcn1	C	A	6: 42,277,275 (GRCm39)	N455K	probably benign	Het
Cpne8	C	T	15: 90,503,893 (GRCm39)	V91I	possibly damaging	Het
Cstdc7	A	G	18: 42,306,727 (GRCm39)		probably null	Het
Ddx1	A	G	12: 13,279,295 (GRCm39)	V448A	probably damaging	Het
Dnhd1	G	A	7: 105,352,416 (GRCm39)	R2523Q	probably damaging	Het
Eeig1	G	A	2: 32,456,339 (GRCm39)	A334T	probably damaging	Het
Eif2a	G	T	3: 58,455,933 (GRCm39)	D311Y	probably damaging	Het
Eif2ak4	G	A	2: 118,258,419 (GRCm39)	E512K	probably damaging	Het
Esp15	A	T	17: 39,955,640 (GRCm39)	Y69F	probably benign	Het
Gucy1a1	A	T	3: 82,016,380 (GRCm39)	Y203N	probably damaging	Het
Hook3	T	C	8: 26,562,321 (GRCm39)	Q248R	probably damaging	Het
Ifit2	T	A	19: 34,550,937 (GRCm39)	V159E	possibly damaging	Het
Il17rd	T	C	14: 26,810,024 (GRCm39)	V20A	possibly damaging	Het
Kcnq3	T	A	15: 65,897,027 (GRCm39)	D291V	probably damaging	Het
Klf11	A	T	12: 24,704,929 (GRCm39)	M111L	probably benign	Het
Lama5	T	A	2: 179,836,356 (GRCm39)	H1165L	probably benign	Het
Lmo7	A	G	14: 102,139,522 (GRCm39)	N702S	probably damaging	Het
Lta4h	T	C	10: 93,307,736 (GRCm39)	V323A	probably damaging	Het
Mkrn3	T	C	7: 62,068,735 (GRCm39)	E352G	possibly damaging	Het
Ms4a19	T	C	19: 11,118,999 (GRCm39)	S37G	possibly damaging	Het
Mtf2	T	A	5: 108,242,023 (GRCm39)	L277Q	probably damaging	Het
Myo9b	A	G	8: 71,775,918 (GRCm39)	N370D	probably benign	Het
Nlrp4e	A	C	7: 23,036,316 (GRCm39)	K723T	probably benign	Het
Nsun3	A	G	16: 62,555,689 (GRCm39)	V279A	possibly damaging	Het
Pde6b	A	G	5: 108,571,424 (GRCm39)	D459G	probably benign	Het
Phgdh	T	C	3: 98,235,655 (GRCm39)	I121V	probably benign	Het
Pik3r5	T	C	11: 68,386,803 (GRCm39)	C811R	probably damaging	Het
Spaca6	A	C	17: 18,051,344 (GRCm39)	I27L	probably benign	Het
Trmt1l	G	A	1: 151,330,746 (GRCm39)	V588I	probably benign	Het
Tshr	A	G	12: 91,503,967 (GRCm39)	N302D	probably damaging	Het
Wnt3a	T	C	11: 59,166,106 (GRCm39)	N58S	probably damaging	Het
Zkscan8	A	G	13: 21,704,895 (GRCm39)	V276A	probably damaging	Het

Other mutations in Vmn2r23

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00324:Vmn2r23	APN	6	123,706,684 (GRCm39)	missense	possibly damaging	0.89
IGL01012:Vmn2r23	APN	6	123,706,555 (GRCm39)	missense	probably benign
IGL01073:Vmn2r23	APN	6	123,689,759 (GRCm39)	missense	possibly damaging	0.82
IGL01547:Vmn2r23	APN	6	123,681,383 (GRCm39)	missense	possibly damaging	0.88
IGL01571:Vmn2r23	APN	6	123,681,366 (GRCm39)	missense	probably damaging	1.00
IGL01950:Vmn2r23	APN	6	123,718,845 (GRCm39)	missense	possibly damaging	0.80
IGL02028:Vmn2r23	APN	6	123,718,819 (GRCm39)	missense	probably damaging	1.00
IGL02248:Vmn2r23	APN	6	123,718,703 (GRCm39)	missense	probably damaging	0.96
IGL02318:Vmn2r23	APN	6	123,718,795 (GRCm39)	missense	probably benign	0.10
IGL02649:Vmn2r23	APN	6	123,681,437 (GRCm39)	missense	probably benign
IGL02831:Vmn2r23	APN	6	123,681,344 (GRCm39)	missense	probably benign	0.22
IGL02832:Vmn2r23	APN	6	123,681,355 (GRCm39)	missense	probably benign	0.00
IGL02865:Vmn2r23	APN	6	123,718,578 (GRCm39)	missense	probably damaging	1.00
IGL02964:Vmn2r23	APN	6	123,718,741 (GRCm39)	missense	possibly damaging	0.93
IGL03347:Vmn2r23	APN	6	123,681,333 (GRCm39)	missense	probably benign	0.01
IGL03396:Vmn2r23	APN	6	123,706,585 (GRCm39)	missense	probably damaging	1.00
PIT4472001:Vmn2r23	UTSW	6	123,689,936 (GRCm39)	missense	possibly damaging	0.62
R0597:Vmn2r23	UTSW	6	123,706,680 (GRCm39)	missense	probably benign	0.08
R0677:Vmn2r23	UTSW	6	123,690,410 (GRCm39)	missense	probably benign	0.00
R0904:Vmn2r23	UTSW	6	123,719,094 (GRCm39)	missense	probably damaging	1.00
R1330:Vmn2r23	UTSW	6	123,718,963 (GRCm39)	missense	probably damaging	1.00
R1424:Vmn2r23	UTSW	6	123,690,229 (GRCm39)	nonsense	probably null
R1629:Vmn2r23	UTSW	6	123,690,386 (GRCm39)	missense	probably benign	0.05
R1842:Vmn2r23	UTSW	6	123,706,649 (GRCm39)	missense	possibly damaging	0.77
R1867:Vmn2r23	UTSW	6	123,679,874 (GRCm39)	missense	probably damaging	1.00
R1919:Vmn2r23	UTSW	6	123,689,969 (GRCm39)	missense	possibly damaging	0.94
R2087:Vmn2r23	UTSW	6	123,718,458 (GRCm39)	missense	probably benign	0.00
R2338:Vmn2r23	UTSW	6	123,681,384 (GRCm39)	missense	possibly damaging	0.88
R2568:Vmn2r23	UTSW	6	123,719,147 (GRCm39)	nonsense	probably null
R2867:Vmn2r23	UTSW	6	123,690,123 (GRCm39)	missense	possibly damaging	0.94
R2867:Vmn2r23	UTSW	6	123,690,123 (GRCm39)	missense	possibly damaging	0.94
R3500:Vmn2r23	UTSW	6	123,690,129 (GRCm39)	missense	possibly damaging	0.81
R3789:Vmn2r23	UTSW	6	123,718,348 (GRCm39)	missense	probably damaging	1.00
R4164:Vmn2r23	UTSW	6	123,706,697 (GRCm39)	missense	probably benign
R4506:Vmn2r23	UTSW	6	123,679,884 (GRCm39)	missense	probably damaging	1.00
R4652:Vmn2r23	UTSW	6	123,718,689 (GRCm39)	missense	probably damaging	1.00
R4697:Vmn2r23	UTSW	6	123,718,785 (GRCm39)	missense	probably damaging	1.00
R4840:Vmn2r23	UTSW	6	123,690,033 (GRCm39)	missense	probably damaging	1.00
R4983:Vmn2r23	UTSW	6	123,710,308 (GRCm39)	missense	probably damaging	1.00
R5276:Vmn2r23	UTSW	6	123,689,936 (GRCm39)	missense	possibly damaging	0.62
R5392:Vmn2r23	UTSW	6	123,681,323 (GRCm39)	missense	probably benign	0.36
R5529:Vmn2r23	UTSW	6	123,690,410 (GRCm39)	missense	probably benign	0.00
R5664:Vmn2r23	UTSW	6	123,690,033 (GRCm39)	missense	probably damaging	1.00
R5749:Vmn2r23	UTSW	6	123,710,232 (GRCm39)	missense	probably benign
R5761:Vmn2r23	UTSW	6	123,689,718 (GRCm39)	missense	probably benign	0.39
R5762:Vmn2r23	UTSW	6	123,710,352 (GRCm39)	missense	probably damaging	1.00
R5868:Vmn2r23	UTSW	6	123,689,901 (GRCm39)	missense	probably benign	0.12
R5935:Vmn2r23	UTSW	6	123,718,854 (GRCm39)	missense	possibly damaging	0.94
R6242:Vmn2r23	UTSW	6	123,681,359 (GRCm39)	missense	possibly damaging	0.82
R6416:Vmn2r23	UTSW	6	123,689,861 (GRCm39)	missense	probably damaging	1.00
R6524:Vmn2r23	UTSW	6	123,690,384 (GRCm39)	missense	probably damaging	1.00
R6576:Vmn2r23	UTSW	6	123,710,232 (GRCm39)	missense	probably benign
R6925:Vmn2r23	UTSW	6	123,681,512 (GRCm39)	missense	probably damaging	1.00
R7148:Vmn2r23	UTSW	6	123,689,981 (GRCm39)	missense	probably benign
R7215:Vmn2r23	UTSW	6	123,681,323 (GRCm39)	missense	probably benign	0.36
R7252:Vmn2r23	UTSW	6	123,718,540 (GRCm39)	missense	probably damaging	0.97
R7403:Vmn2r23	UTSW	6	123,681,538 (GRCm39)	missense	probably benign	0.01
R8015:Vmn2r23	UTSW	6	123,681,500 (GRCm39)	missense	probably benign	0.00
R8143:Vmn2r23	UTSW	6	123,718,312 (GRCm39)	missense	probably damaging	0.99
R8474:Vmn2r23	UTSW	6	123,681,599 (GRCm39)	missense	probably benign	0.36
R8520:Vmn2r23	UTSW	6	123,718,615 (GRCm39)	missense	probably damaging	0.99
R8679:Vmn2r23	UTSW	6	123,690,431 (GRCm39)	missense	probably damaging	0.99
R8713:Vmn2r23	UTSW	6	123,679,991 (GRCm39)	missense
R8966:Vmn2r23	UTSW	6	123,719,079 (GRCm39)	missense	possibly damaging	0.94
R9124:Vmn2r23	UTSW	6	123,719,038 (GRCm39)	missense	possibly damaging	0.57
R9163:Vmn2r23	UTSW	6	123,718,782 (GRCm39)	missense	probably damaging	1.00
R9189:Vmn2r23	UTSW	6	123,681,323 (GRCm39)	missense	probably benign	0.36
R9451:Vmn2r23	UTSW	6	123,710,352 (GRCm39)	missense	probably damaging	1.00
R9495:Vmn2r23	UTSW	6	123,689,672 (GRCm39)	missense	probably benign	0.30
R9514:Vmn2r23	UTSW	6	123,689,672 (GRCm39)	missense	probably benign	0.30
RF018:Vmn2r23	UTSW	6	123,690,075 (GRCm39)	missense	probably benign	0.00
T0975:Vmn2r23	UTSW	6	123,690,120 (GRCm39)	missense	probably benign	0.00
Z1088:Vmn2r23	UTSW	6	123,719,067 (GRCm39)	missense	probably damaging	0.98
Z1177:Vmn2r23	UTSW	6	123,706,684 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ATATGCGGGGAGAACTCTTCC -3'
(R):5'- ATGGACCACACATCCTGGATAAAG -3'

Sequencing Primer
(F):5'- GGAGAACTCTTCCTTAGGGACATC -3'
(R):5'- GGTTGAACGTCTCTGAGAAAATCC -3'

Posted On

2016-10-06