Incidental Mutation 'R5479:Dgka'
ID 434227
Institutional Source Beutler Lab
Gene Symbol Dgka
Ensembl Gene ENSMUSG00000025357
Gene Name diacylglycerol kinase, alpha
Synonyms Dagk1
MMRRC Submission 043040-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.366) question?
Stock # R5479 (G1)
Quality Score 225
Status Not validated
Chromosome 10
Chromosomal Location 128556003-128580724 bp(-) (GRCm39)
Type of Mutation critical splice acceptor site
DNA Base Change (assembly) T to A at 128565541 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000026414 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000026414] [ENSMUST00000026414] [ENSMUST00000219834]
AlphaFold O88673
Predicted Effect probably null
Transcript: ENSMUST00000026414
SMART Domains Protein: ENSMUSP00000026414
Gene: ENSMUSG00000025357

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 4 93 6.9e-31 PFAM
EFh 115 143 3.82e0 SMART
EFh 160 188 1.29e-4 SMART
C1 207 254 2.29e-10 SMART
C1 269 320 6.91e-5 SMART
DAGKc 372 495 3.11e-62 SMART
DAGKa 515 696 4.1e-103 SMART
Predicted Effect probably null
Transcript: ENSMUST00000026414
SMART Domains Protein: ENSMUSP00000026414
Gene: ENSMUSG00000025357

DomainStartEndE-ValueType
Pfam:DAG_kinase_N 4 93 6.9e-31 PFAM
EFh 115 143 3.82e0 SMART
EFh 160 188 1.29e-4 SMART
C1 207 254 2.29e-10 SMART
C1 269 320 6.91e-5 SMART
DAGKc 372 495 3.11e-62 SMART
DAGKa 515 696 4.1e-103 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000217957
Predicted Effect probably benign
Transcript: ENSMUST00000219834
Coding Region Coverage
  • 1x: 98.4%
  • 3x: 97.4%
  • 10x: 95.4%
  • 20x: 91.3%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutation of this gene results in impaired induction of T cell anergy. T cells stimulated in anergy producing conditions show increased proliferation and interleukin 2 production. Mice homozygous for a transgenic gene disruption exhibit male infertility. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 45 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acsl5 G T 19: 55,268,894 (GRCm39) C185F probably damaging Het
Adad2 C A 8: 120,341,654 (GRCm39) T185N possibly damaging Het
Apbb1 A T 7: 105,214,232 (GRCm39) V315E probably damaging Het
Arfrp1 T C 2: 181,006,191 (GRCm39) T32A probably damaging Het
B020004C17Rik T C 14: 57,253,999 (GRCm39) S41P probably benign Het
Celsr3 T C 9: 108,721,743 (GRCm39) probably null Het
Chd8 C T 14: 52,452,652 (GRCm39) G1289D probably benign Het
Ctrl A G 8: 106,659,672 (GRCm39) F59S probably damaging Het
Cyp4v3 T A 8: 45,763,243 (GRCm39) D356V probably benign Het
Dnah7b T C 1: 46,262,265 (GRCm39) Y2043H probably damaging Het
Eya3 A G 4: 132,400,244 (GRCm39) T88A possibly damaging Het
Fat1 A G 8: 45,489,912 (GRCm39) D3608G possibly damaging Het
Fmo1 T C 1: 162,677,793 (GRCm39) T108A probably damaging Het
Frs3 A G 17: 48,012,635 (GRCm39) E114G probably damaging Het
Ftcd A T 10: 76,413,850 (GRCm39) M85L probably benign Het
Ggnbp2 T C 11: 84,745,169 (GRCm39) S189G probably benign Het
Gm3629 C T 14: 17,875,765 (GRCm39) R35H unknown Het
Gnai2 T G 9: 107,512,365 (GRCm39) M18L probably benign Het
Gpx5 T C 13: 21,476,805 (GRCm39) Y9C probably benign Het
Grik1 C A 16: 87,732,914 (GRCm39) A668S probably damaging Het
Hectd4 A T 5: 121,445,011 (GRCm39) M83L probably benign Het
Klra9 T C 6: 130,156,075 (GRCm39) R227G probably benign Het
Maz A T 7: 126,624,860 (GRCm39) V197D possibly damaging Het
Mbd5 T C 2: 49,162,917 (GRCm39) V241A probably damaging Het
Mdga2 C T 12: 66,701,950 (GRCm39) C417Y probably damaging Het
Mios C T 6: 8,215,314 (GRCm39) T170I probably benign Het
Myh13 G A 11: 67,239,648 (GRCm39) A733T probably damaging Het
Ncam2 C T 16: 81,231,766 (GRCm39) R77* probably null Het
Nfs1 G A 2: 155,970,422 (GRCm39) T297I probably damaging Het
Nrbf2 A G 10: 67,120,870 (GRCm39) probably null Het
Nrg1 T C 8: 32,308,405 (GRCm39) Y537C probably damaging Het
Onecut3 T A 10: 80,349,856 (GRCm39) L450Q probably damaging Het
Or4p8 T C 2: 88,727,035 (GRCm39) D302G probably benign Het
Pard3 G A 8: 128,096,836 (GRCm39) R333Q probably damaging Het
Parp4 T C 14: 56,861,552 (GRCm39) F962L probably benign Het
Pilra A G 5: 137,834,318 (GRCm39) S22P possibly damaging Het
Piwil4 T C 9: 14,616,337 (GRCm39) Y673C probably damaging Het
Plxna2 G A 1: 194,476,181 (GRCm39) D1134N probably benign Het
Pnpla7 T C 2: 24,909,453 (GRCm39) V681A possibly damaging Het
Ppfibp1 T A 6: 146,931,648 (GRCm39) probably null Het
Ptprz1 C T 6: 23,001,665 (GRCm39) L1252F probably benign Het
Sptb T C 12: 76,646,625 (GRCm39) E1999G probably benign Het
Stat3 T C 11: 100,780,714 (GRCm39) probably benign Het
Usp25 T C 16: 76,904,801 (GRCm39) V789A possibly damaging Het
Zfp148 C A 16: 33,317,589 (GRCm39) Q712K probably damaging Het
Other mutations in Dgka
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00089:Dgka APN 10 128,568,955 (GRCm39) missense probably damaging 1.00
IGL02479:Dgka APN 10 128,566,115 (GRCm39) missense probably benign 0.01
IGL02727:Dgka APN 10 128,558,317 (GRCm39) splice site probably benign
IGL02817:Dgka APN 10 128,566,097 (GRCm39) missense probably benign
IGL02882:Dgka APN 10 128,569,253 (GRCm39) missense possibly damaging 0.77
IGL03239:Dgka APN 10 128,557,254 (GRCm39) splice site probably benign
Caps UTSW 10 128,566,071 (GRCm39) nonsense probably null
Greenie UTSW 10 128,568,962 (GRCm39) missense probably benign 0.03
Hangup UTSW 10 128,556,356 (GRCm39) missense probably damaging 1.00
Stickem UTSW 10 128,559,548 (GRCm39) missense probably damaging 1.00
R0321:Dgka UTSW 10 128,556,952 (GRCm39) splice site probably benign
R0374:Dgka UTSW 10 128,556,952 (GRCm39) splice site probably benign
R0482:Dgka UTSW 10 128,569,990 (GRCm39) nonsense probably null
R0494:Dgka UTSW 10 128,556,952 (GRCm39) splice site probably benign
R0573:Dgka UTSW 10 128,572,876 (GRCm39) critical splice donor site probably null
R0594:Dgka UTSW 10 128,568,979 (GRCm39) splice site probably benign
R0607:Dgka UTSW 10 128,556,338 (GRCm39) splice site probably null
R0618:Dgka UTSW 10 128,556,952 (GRCm39) splice site probably benign
R0691:Dgka UTSW 10 128,559,129 (GRCm39) splice site probably benign
R1378:Dgka UTSW 10 128,571,696 (GRCm39) splice site probably null
R1424:Dgka UTSW 10 128,569,202 (GRCm39) missense possibly damaging 0.57
R1955:Dgka UTSW 10 128,566,058 (GRCm39) critical splice donor site probably null
R1972:Dgka UTSW 10 128,556,335 (GRCm39) missense probably damaging 0.99
R1998:Dgka UTSW 10 128,565,808 (GRCm39) missense probably benign 0.00
R2046:Dgka UTSW 10 128,559,404 (GRCm39) missense probably damaging 1.00
R4206:Dgka UTSW 10 128,557,064 (GRCm39) missense probably damaging 1.00
R4418:Dgka UTSW 10 128,563,963 (GRCm39) missense probably damaging 1.00
R4752:Dgka UTSW 10 128,572,528 (GRCm39) missense probably benign 0.03
R5092:Dgka UTSW 10 128,571,702 (GRCm39) missense probably damaging 0.99
R6009:Dgka UTSW 10 128,559,548 (GRCm39) missense probably damaging 1.00
R6273:Dgka UTSW 10 128,559,515 (GRCm39) missense probably benign 0.03
R6852:Dgka UTSW 10 128,558,408 (GRCm39) missense probably damaging 1.00
R6947:Dgka UTSW 10 128,568,884 (GRCm39) missense probably damaging 1.00
R6973:Dgka UTSW 10 128,565,463 (GRCm39) splice site probably null
R7024:Dgka UTSW 10 128,556,356 (GRCm39) missense probably damaging 1.00
R7076:Dgka UTSW 10 128,569,452 (GRCm39) missense probably damaging 0.99
R7290:Dgka UTSW 10 128,569,468 (GRCm39) missense probably damaging 0.99
R7397:Dgka UTSW 10 128,556,594 (GRCm39) missense possibly damaging 0.95
R7823:Dgka UTSW 10 128,572,135 (GRCm39) missense probably benign 0.00
R7856:Dgka UTSW 10 128,572,533 (GRCm39) missense probably benign
R8118:Dgka UTSW 10 128,558,318 (GRCm39) splice site probably null
R8360:Dgka UTSW 10 128,563,997 (GRCm39) missense probably damaging 0.99
R8374:Dgka UTSW 10 128,557,112 (GRCm39) missense probably benign 0.01
R8547:Dgka UTSW 10 128,556,881 (GRCm39) missense probably damaging 1.00
R8686:Dgka UTSW 10 128,568,962 (GRCm39) missense probably benign 0.03
R9013:Dgka UTSW 10 128,566,071 (GRCm39) nonsense probably null
R9307:Dgka UTSW 10 128,567,046 (GRCm39) missense probably damaging 1.00
R9336:Dgka UTSW 10 128,566,935 (GRCm39) critical splice donor site probably null
R9423:Dgka UTSW 10 128,557,055 (GRCm39) missense probably damaging 0.96
X0020:Dgka UTSW 10 128,557,186 (GRCm39) missense probably damaging 1.00
Z1177:Dgka UTSW 10 128,556,337 (GRCm39) missense probably benign 0.00
Z1177:Dgka UTSW 10 128,567,034 (GRCm39) missense possibly damaging 0.91
Predicted Primers PCR Primer
(F):5'- GGGTCTCCTATCCTAACAGACAG -3'
(R):5'- AGGCCTATGCTGGTTCTTCCTAG -3'

Sequencing Primer
(F):5'- ATCCTAACAGACAGTCTCTCTCTC -3'
(R):5'- TCCTAGATGGAGAGAGAGGATC -3'
Posted On 2016-10-06