Mutagenetix > Incidental Mutation

Incidental Mutation 'R5480:Vmn2r60'

434258

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r60

Ensembl Gene

ENSMUSG00000090619

Gene Name

vomeronasal 2, receptor 60

Synonyms

Casr-rs3, EG637898, Gprc2a-rs3

MMRRC Submission

043041-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.112) question?

Stock #

R5480 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

41765895-41845200 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 41785154 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Leucine at position 122 (W122L)

Ref Sequence

ENSEMBL: ENSMUSP00000128493 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166447]

AlphaFold

A0A3B2WBC8

Predicted Effect

probably damaging
Transcript: ENSMUST00000166447
AA Change: W122L

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000128493
Gene: ENSMUSG00000090619
AA Change: W122L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	78	471	1.2e-44	PFAM
Pfam:NCD3G	514	567	5.1e-23	PFAM
Pfam:7tm_3	600	835	1.4e-51	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 98.3%
3x: 97.3%
10x: 95.2%
20x: 90.7%

Validation Efficiency

97% (56/58)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alkbh7	A	G	17: 57,306,131 (GRCm39)		probably benign	Het
Alpk2	A	G	18: 65,482,979 (GRCm39)	L343S	probably damaging	Het
Btbd10	T	A	7: 112,915,914 (GRCm39)	R392W	probably damaging	Het
Camkv	A	G	9: 107,824,074 (GRCm39)	D216G	probably damaging	Het
Col22a1	T	C	15: 71,836,460 (GRCm39)	D525G	probably damaging	Het
Dqx1	A	T	6: 83,041,784 (GRCm39)	D542V	probably damaging	Het
Epha8	T	C	4: 136,662,441 (GRCm39)	T539A	probably benign	Het
Faap100	T	C	11: 120,267,939 (GRCm39)	E278G	probably damaging	Het
Fat2	A	G	11: 55,200,912 (GRCm39)	S721P	probably damaging	Het
Fcgbpl1	T	A	7: 27,857,424 (GRCm39)	C2257*	probably null	Het
Frem2	A	T	3: 53,563,928 (GRCm39)	L193*	probably null	Het
Gfy	C	A	7: 44,826,657 (GRCm39)	V394F	probably benign	Het
Gipr	A	G	7: 18,894,579 (GRCm39)	L241P	probably damaging	Het
Gm5478	A	G	15: 101,552,100 (GRCm39)	S445P	probably damaging	Het
Ift140	G	T	17: 25,239,550 (GRCm39)	W69L	probably damaging	Het
Kat6a	T	A	8: 23,428,323 (GRCm39)	M1226K	possibly damaging	Het
Klk12	T	G	7: 43,420,482 (GRCm39)	H140Q	probably benign	Het
Map3k14	A	G	11: 103,130,330 (GRCm39)	F196L	probably benign	Het
Mblac2	T	A	13: 81,898,395 (GRCm39)	V257E	possibly damaging	Het
Pcdha11	A	G	18: 37,138,935 (GRCm39)	E188G	probably benign	Het
Pdzd7	T	C	19: 45,027,724 (GRCm39)	N250S	possibly damaging	Het
Phkb	A	G	8: 86,648,811 (GRCm39)	D209G	probably damaging	Het
Pigs	T	G	11: 78,219,901 (GRCm39)	I92S	possibly damaging	Het
Pigz	G	T	16: 31,763,439 (GRCm39)	G166C	probably damaging	Het
Pkd1l2	C	A	8: 117,757,388 (GRCm39)	R1550L	probably damaging	Het
Pkd2l1	A	G	19: 44,180,595 (GRCm39)	V40A	probably benign	Het
Plxna1	A	T	6: 89,301,616 (GRCm39)	M1470K	probably damaging	Het
Polq	T	A	16: 36,833,652 (GRCm39)		probably benign	Het
Prune2	A	G	19: 17,098,311 (GRCm39)	T1272A	possibly damaging	Het
Rfwd3	A	T	8: 112,000,464 (GRCm39)	D720E	probably damaging	Het
Rgs12	C	G	5: 35,123,455 (GRCm39)	Q413E	probably benign	Het
Rhobtb1	T	A	10: 69,106,563 (GRCm39)	V376D	possibly damaging	Het
Rrp8	C	T	7: 105,383,336 (GRCm39)	S310N	probably damaging	Het
S100a3	C	T	3: 90,509,591 (GRCm39)	L79F	probably damaging	Het
Setbp1	C	A	18: 78,901,278 (GRCm39)	M796I	probably damaging	Het
Sipa1	A	G	19: 5,709,658 (GRCm39)	L254P	possibly damaging	Het
Slc4a7	C	G	14: 14,782,138 (GRCm38)	H964Q	probably damaging	Het
Strc	G	T	2: 121,195,300 (GRCm39)	P1661Q	probably benign	Het
Taf5l	A	T	8: 124,736,559 (GRCm39)	V4E	possibly damaging	Het
Tbc1d15	T	C	10: 115,069,123 (GRCm39)	E82G	probably damaging	Het
Thada	A	G	17: 84,739,682 (GRCm39)	S858P	probably benign	Het
Ticrr	T	C	7: 79,310,557 (GRCm39)	V157A	probably damaging	Het
Trim21	T	C	7: 102,208,463 (GRCm39)	T419A	probably benign	Het
Vwa3b	C	A	1: 37,139,787 (GRCm39)	Y369*	probably null	Het
Ythdf3	T	C	3: 16,237,664 (GRCm39)	S2P	possibly damaging	Het

Other mutations in Vmn2r60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01622:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL01623:Vmn2r60	APN	7	41,785,910 (GRCm39)	missense	probably benign	0.09
IGL02363:Vmn2r60	APN	7	41,844,578 (GRCm39)	missense	probably benign	0.02
IGL02485:Vmn2r60	APN	7	41,844,890 (GRCm39)	missense	possibly damaging	0.54
IGL02651:Vmn2r60	APN	7	41,845,010 (GRCm39)	missense	probably damaging	0.99
IGL02660:Vmn2r60	APN	7	41,791,720 (GRCm39)	nonsense	probably null
IGL03135:Vmn2r60	APN	7	41,786,018 (GRCm39)	missense	probably benign	0.13
IGL03307:Vmn2r60	APN	7	41,765,971 (GRCm39)	missense	probably benign	0.14
R0310:Vmn2r60	UTSW	7	41,844,564 (GRCm39)	missense	possibly damaging	0.54
R0314:Vmn2r60	UTSW	7	41,784,985 (GRCm39)	splice site	probably benign
R0328:Vmn2r60	UTSW	7	41,791,744 (GRCm39)	splice site	probably benign
R0464:Vmn2r60	UTSW	7	41,785,255 (GRCm39)	missense	probably damaging	0.99
R0755:Vmn2r60	UTSW	7	41,844,869 (GRCm39)	missense	probably damaging	1.00
R1119:Vmn2r60	UTSW	7	41,844,365 (GRCm39)	missense	possibly damaging	0.68
R1162:Vmn2r60	UTSW	7	41,845,195 (GRCm39)	missense	probably benign	0.29
R1241:Vmn2r60	UTSW	7	41,786,476 (GRCm39)	missense	probably benign	0.01
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1404:Vmn2r60	UTSW	7	41,786,211 (GRCm39)	missense	probably damaging	0.99
R1488:Vmn2r60	UTSW	7	41,786,137 (GRCm39)	missense	probably benign	0.17
R1623:Vmn2r60	UTSW	7	41,785,279 (GRCm39)	nonsense	probably null
R1628:Vmn2r60	UTSW	7	41,785,830 (GRCm39)	nonsense	probably null
R1883:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R1884:Vmn2r60	UTSW	7	41,786,094 (GRCm39)	missense	probably damaging	0.99
R2182:Vmn2r60	UTSW	7	41,844,931 (GRCm39)	missense	probably benign	0.06
R2275:Vmn2r60	UTSW	7	41,786,251 (GRCm39)	nonsense	probably null
R2847:Vmn2r60	UTSW	7	41,785,857 (GRCm39)	missense	probably benign	0.07
R2885:Vmn2r60	UTSW	7	41,790,403 (GRCm39)	missense	possibly damaging	0.91
R2894:Vmn2r60	UTSW	7	41,785,220 (GRCm39)	missense	probably benign
R2921:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R2922:Vmn2r60	UTSW	7	41,790,459 (GRCm39)	missense	probably damaging	0.98
R3772:Vmn2r60	UTSW	7	41,765,980 (GRCm39)	missense	probably benign	0.35
R3820:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3822:Vmn2r60	UTSW	7	41,785,125 (GRCm39)	missense	probably damaging	0.98
R3872:Vmn2r60	UTSW	7	41,785,878 (GRCm39)	missense	probably benign	0.19
R4222:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4223:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4224:Vmn2r60	UTSW	7	41,765,952 (GRCm39)	missense	probably benign	0.08
R4526:Vmn2r60	UTSW	7	41,844,667 (GRCm39)	missense	probably damaging	0.96
R4547:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R4840:Vmn2r60	UTSW	7	41,785,285 (GRCm39)	missense	probably damaging	1.00
R5173:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.97
R5231:Vmn2r60	UTSW	7	41,786,448 (GRCm39)	missense	possibly damaging	0.93
R5521:Vmn2r60	UTSW	7	41,845,049 (GRCm39)	missense	probably damaging	0.99
R5834:Vmn2r60	UTSW	7	41,765,932 (GRCm39)	missense	probably benign	0.17
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6038:Vmn2r60	UTSW	7	41,844,386 (GRCm39)	missense	probably benign	0.04
R6112:Vmn2r60	UTSW	7	41,844,847 (GRCm39)	missense	probably damaging	1.00
R6149:Vmn2r60	UTSW	7	41,786,400 (GRCm39)	missense	probably damaging	1.00
R6170:Vmn2r60	UTSW	7	41,785,045 (GRCm39)	missense	possibly damaging	0.94
R6383:Vmn2r60	UTSW	7	41,765,895 (GRCm39)	start codon destroyed	probably null	0.04
R6811:Vmn2r60	UTSW	7	41,844,310 (GRCm39)	missense	probably damaging	1.00
R6876:Vmn2r60	UTSW	7	41,785,087 (GRCm39)	missense	probably null	0.54
R6997:Vmn2r60	UTSW	7	41,791,716 (GRCm39)	missense	probably benign	0.00
R7040:Vmn2r60	UTSW	7	41,791,666 (GRCm39)	missense	probably benign	0.00
R7116:Vmn2r60	UTSW	7	41,786,487 (GRCm39)	missense	probably benign	0.00
R7128:Vmn2r60	UTSW	7	41,844,536 (GRCm39)	missense	probably damaging	0.96
R7232:Vmn2r60	UTSW	7	41,786,166 (GRCm39)	missense	possibly damaging	0.83
R7296:Vmn2r60	UTSW	7	41,785,826 (GRCm39)	missense	probably benign	0.01
R7376:Vmn2r60	UTSW	7	41,844,631 (GRCm39)	missense	probably damaging	1.00
R7526:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7527:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7528:Vmn2r60	UTSW	7	41,845,158 (GRCm39)	frame shift	probably null
R7764:Vmn2r60	UTSW	7	41,844,535 (GRCm39)	missense	probably damaging	0.99
R7843:Vmn2r60	UTSW	7	41,844,511 (GRCm39)	missense	probably benign	0.00
R8080:Vmn2r60	UTSW	7	41,790,521 (GRCm39)	missense	probably benign	0.30
R8290:Vmn2r60	UTSW	7	41,791,690 (GRCm39)	missense	probably damaging	1.00
R8342:Vmn2r60	UTSW	7	41,790,494 (GRCm39)	missense	possibly damaging	0.63
R8362:Vmn2r60	UTSW	7	41,844,954 (GRCm39)	missense	probably damaging	1.00
R8418:Vmn2r60	UTSW	7	41,844,850 (GRCm39)	missense	probably damaging	0.97
R8848:Vmn2r60	UTSW	7	41,786,169 (GRCm39)	missense	probably damaging	1.00
R8860:Vmn2r60	UTSW	7	41,791,654 (GRCm39)	missense	probably damaging	0.99
R8882:Vmn2r60	UTSW	7	41,790,518 (GRCm39)	missense	probably benign	0.00
R8913:Vmn2r60	UTSW	7	41,785,778 (GRCm39)	missense	probably benign	0.27
R9190:Vmn2r60	UTSW	7	41,844,935 (GRCm39)	missense	probably damaging	0.99
R9229:Vmn2r60	UTSW	7	41,791,723 (GRCm39)	missense	possibly damaging	0.95
R9295:Vmn2r60	UTSW	7	41,785,955 (GRCm39)	missense	probably benign	0.01
R9335:Vmn2r60	UTSW	7	41,844,332 (GRCm39)	missense	probably damaging	1.00
R9796:Vmn2r60	UTSW	7	41,785,172 (GRCm39)	missense	probably benign
RF024:Vmn2r60	UTSW	7	41,790,363 (GRCm39)	missense	probably benign	0.01
X0023:Vmn2r60	UTSW	7	41,790,538 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCACTGCGCTAAATAGAAAAGTTG -3'
(R):5'- AGGGTACTTGTTTCCTAGGTCC -3'

Sequencing Primer
(F):5'- AGAAAAGTTGTATGGTCTATGGCTTC -3'
(R):5'- GGTACTTGTTTCCTAGGTCCTACATC -3'

Posted On

2016-10-06