Incidental Mutation 'R5535:Mmp25'
ID434797
Institutional Source Beutler Lab
Gene Symbol Mmp25
Ensembl Gene ENSMUSG00000023903
Gene Namematrix metallopeptidase 25
SynonymsLeukolysin, MT6-MMP, F730048C11Rik
MMRRC Submission 043093-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.082) question?
Stock #R5535 (G1)
Quality Score183
Status Not validated
Chromosome17
Chromosomal Location23629458-23645269 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 23644760 bp
ZygosityHeterozygous
Amino Acid Change Leucine to Proline at position 32 (L32P)
Ref Sequence ENSEMBL: ENSMUSP00000024696 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000024696]
Predicted Effect probably benign
Transcript: ENSMUST00000024696
AA Change: L32P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
SMART Domains Protein: ENSMUSP00000024696
Gene: ENSMUSG00000023903
AA Change: L32P

DomainStartEndE-ValueType
low complexity region 54 75 N/A INTRINSIC
Pfam:PG_binding_1 82 140 8.8e-12 PFAM
ZnMc 166 335 1.68e-47 SMART
low complexity region 343 369 N/A INTRINSIC
HX 375 419 6.35e-8 SMART
HX 424 466 1.62e-5 SMART
HX 470 516 1.64e-10 SMART
HX 518 562 2.79e-4 SMART
low complexity region 572 581 N/A INTRINSIC
low complexity region 597 607 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.3%
  • 20x: 97.6%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded precursor undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
Allele List at MGI
Other mutations in this stock
Total: 39 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acaa1b C T 9: 119,148,406 G403S probably damaging Het
Agbl2 G A 2: 90,810,006 V699I probably benign Het
Amer2 AAGGAGGAGGAGGAG AAGGAGGAGGAG 14: 60,378,853 probably benign Het
Bace2 A T 16: 97,413,425 Q271L probably damaging Het
Btn2a2 T C 13: 23,478,275 K493E probably benign Het
Ces3a T A 8: 105,051,564 D222E probably benign Het
Ckap2l A G 2: 129,285,842 C139R probably benign Het
Clip4 A G 17: 71,831,262 H485R probably benign Het
Cntfr T A 4: 41,663,216 D197V probably benign Het
Efcab5 A G 11: 77,151,921 L2P probably damaging Het
Elmo2 A G 2: 165,310,212 V163A possibly damaging Het
Fkbpl G A 17: 34,645,329 A24T probably benign Het
Flrt2 A G 12: 95,780,426 T513A probably benign Het
Gm10801 GT GTTTTT 2: 98,662,499 probably null Het
Hectd1 A T 12: 51,802,326 F332I probably damaging Het
Helz A G 11: 107,646,120 D947G probably damaging Het
Hivep2 T A 10: 14,131,022 D1121E probably benign Het
Hoxa13 GG GGCG 6: 52,260,540 probably null Homo
Hoxd13 A T 2: 74,668,797 Y163F probably damaging Het
Immt C A 6: 71,852,784 P158Q probably null Het
Kcnh5 A G 12: 75,130,907 S142P possibly damaging Het
Lnpep T G 17: 17,538,694 H796P probably benign Het
Mfhas1 C A 8: 35,590,269 R633S possibly damaging Het
Myo15b A G 11: 115,881,301 D299G probably damaging Het
Myo18b T C 5: 112,790,042 E1739G probably damaging Het
Olfr907 T A 9: 38,498,998 S110T probably benign Het
Parp9 A G 16: 35,956,825 K147E probably damaging Het
Pcdha3 G T 18: 36,947,936 R577L probably benign Het
Plod2 T A 9: 92,606,569 I637N probably damaging Het
Polk T G 13: 96,495,497 S243R probably damaging Het
Prag1 T C 8: 36,104,014 S584P probably benign Het
Prex1 A T 2: 166,580,273 V43E possibly damaging Het
Rdh10 T C 1: 16,131,184 Y294H probably damaging Het
Rnf126 T A 10: 79,762,699 I28F probably damaging Het
Sdk2 T A 11: 113,943,158 H66L possibly damaging Het
Tet1 G T 10: 62,832,907 P1431Q probably damaging Het
Tmco2 T A 4: 121,105,993 Q103L possibly damaging Het
Ucp3 A T 7: 100,480,666 R172W probably benign Het
Unc79 A T 12: 103,169,703 I2270F possibly damaging Het
Other mutations in Mmp25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02814:Mmp25 APN 17 23639762 missense probably damaging 1.00
IGL02893:Mmp25 APN 17 23644051 missense probably damaging 1.00
R0471:Mmp25 UTSW 17 23639884 missense possibly damaging 0.73
R0478:Mmp25 UTSW 17 23632782 missense probably benign 0.08
R1829:Mmp25 UTSW 17 23640023 missense probably benign 0.00
R2005:Mmp25 UTSW 17 23640242 missense probably damaging 1.00
R2151:Mmp25 UTSW 17 23631074 missense probably damaging 1.00
R2153:Mmp25 UTSW 17 23631074 missense probably damaging 1.00
R2154:Mmp25 UTSW 17 23631074 missense probably damaging 1.00
R2937:Mmp25 UTSW 17 23644791 missense probably benign 0.00
R4418:Mmp25 UTSW 17 23644070 missense probably damaging 1.00
R4667:Mmp25 UTSW 17 23644607 missense probably benign 0.00
R4905:Mmp25 UTSW 17 23644048 nonsense probably null
R5592:Mmp25 UTSW 17 23640202 missense possibly damaging 0.95
R5888:Mmp25 UTSW 17 23631074 missense probably damaging 1.00
R6261:Mmp25 UTSW 17 23630794 missense possibly damaging 0.61
R6263:Mmp25 UTSW 17 23630794 missense possibly damaging 0.61
R6264:Mmp25 UTSW 17 23630794 missense possibly damaging 0.61
R6571:Mmp25 UTSW 17 23639896 missense probably benign 0.17
R7172:Mmp25 UTSW 17 23644788 missense probably benign
R7467:Mmp25 UTSW 17 23644782 missense possibly damaging 0.53
R8109:Mmp25 UTSW 17 23644794 missense probably benign 0.00
T0722:Mmp25 UTSW 17 23631218 missense possibly damaging 0.94
Z1176:Mmp25 UTSW 17 23630659 missense probably damaging 1.00
Z1177:Mmp25 UTSW 17 23644137 missense possibly damaging 0.76
Predicted Primers PCR Primer
(F):5'- TTCTGTGTGATCCCAGTTGC -3'
(R):5'- AGCATATTGGGCAGTTGGC -3'

Sequencing Primer
(F):5'- ATCCCAGTTGCGGGTGGAC -3'
(R):5'- GACCTCTTTGCCGGACC -3'
Posted On2016-10-24