Mutagenetix > Incidental Mutation

Incidental Mutation 'R5553:Vmn2r100'

435242

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r100

Ensembl Gene

ENSMUSG00000091859

Gene Name

vomeronasal 2, receptor 100

Synonyms

EG627537

MMRRC Submission

043110-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R5553 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19725073-19752322 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19725110 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Arginine at position 13 (Q13R)

Ref Sequence

ENSEMBL: ENSMUSP00000156263 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166081] [ENSMUST00000231465]

AlphaFold

E9QAZ9

Predicted Effect

probably benign
Transcript: ENSMUST00000166081
AA Change: Q13R

PolyPhen 2 Score 0.317 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000128350
Gene: ENSMUSG00000091859
AA Change: Q13R

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	83	456	7.4e-41	PFAM
Pfam:NCD3G	510	563	1.9e-21	PFAM
Pfam:7tm_3	594	831	2.6e-51	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000231465
AA Change: Q13R

PolyPhen 2 Score 0.642 (Sensitivity: 0.87; Specificity: 0.91)

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A1bg	T	A	15: 60,792,690 (GRCm39)	I86F	probably damaging	Het
Abca13	C	T	11: 9,278,158 (GRCm39)	L3113F	probably damaging	Het
Ankrd39	C	T	1: 36,581,062 (GRCm39)	G96R	probably damaging	Het
Ano8	T	A	8: 71,937,641 (GRCm39)		probably null	Het
Arid1b	A	T	17: 5,364,152 (GRCm39)	S1041C	probably damaging	Het
Bsn	T	A	9: 107,987,620 (GRCm39)		probably benign	Het
Cbr3	A	G	16: 93,480,451 (GRCm39)	E80G	possibly damaging	Het
Chd1	A	G	17: 17,605,875 (GRCm39)	E271G	probably benign	Het
Dock3	T	A	9: 106,868,309 (GRCm39)	K658N	possibly damaging	Het
Dot1l	CCAGCCCCACCCTCAGCC	CCAGCC	10: 80,619,462 (GRCm39)		probably benign	Het
Dppa1	T	A	11: 46,503,861 (GRCm39)		probably null	Het
Fen1	T	C	19: 10,177,787 (GRCm39)	N219S	probably benign	Het
Fsip2	A	G	2: 82,793,090 (GRCm39)	T416A	probably benign	Het
Gm14393	A	T	2: 174,903,639 (GRCm39)	C89*	probably null	Het
Grin2c	C	T	11: 115,143,551 (GRCm39)	M736I	probably null	Het
Heatr5b	A	T	17: 79,060,780 (GRCm39)		probably null	Het
Hspbap1	G	T	16: 35,621,967 (GRCm39)	W104L	probably damaging	Het
Igfn1	C	T	1: 135,895,622 (GRCm39)	G1648E	probably damaging	Het
Irf4	A	G	13: 30,935,811 (GRCm39)	Y122C	probably damaging	Het
Kremen2	A	G	17: 23,960,776 (GRCm39)		probably benign	Het
Niban1	C	T	1: 151,592,986 (GRCm39)	T557M	probably damaging	Het
Nubpl	T	A	12: 52,228,082 (GRCm39)	L169M	possibly damaging	Het
Nwd1	T	C	8: 73,431,604 (GRCm39)	S1200P	possibly damaging	Het
Or1j20	A	G	2: 36,760,477 (GRCm39)	I300V	probably benign	Het
Or5p61	A	T	7: 107,758,478 (GRCm39)	S201T	probably benign	Het
Parp14	G	T	16: 35,677,306 (GRCm39)	H887Q	probably benign	Het
Paxip1	G	A	5: 27,980,637 (GRCm39)		probably benign	Het
Piwil1	T	C	5: 128,822,565 (GRCm39)	M392T	probably benign	Het
Plekhm3	T	C	1: 64,961,045 (GRCm39)	S404G	possibly damaging	Het
Prelid3a	T	C	18: 67,610,093 (GRCm39)	L141P	probably damaging	Het
Ptprb	T	A	10: 116,186,090 (GRCm39)	V1715E	probably damaging	Het
Rc3h2	G	A	2: 37,288,323 (GRCm39)	R420*	probably null	Het
Selenon	C	A	4: 134,268,228 (GRCm39)	R435L	probably damaging	Het
Slc29a4	T	C	5: 142,705,791 (GRCm39)	L425P	probably damaging	Het
Slc30a9	T	A	5: 67,502,947 (GRCm39)		probably null	Het
Slc9a5	T	C	8: 106,083,672 (GRCm39)	V404A	probably damaging	Het
Ssc5d	A	T	7: 4,939,289 (GRCm39)	D575V	probably damaging	Het
Ttn	A	C	2: 76,721,940 (GRCm39)		probably null	Het
Wfikkn1	T	A	17: 26,097,468 (GRCm39)	L285F	possibly damaging	Het
Zcchc17	A	G	4: 130,247,927 (GRCm39)		probably null	Het

Other mutations in Vmn2r100

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00904:Vmn2r100	APN	17	19,746,262 (GRCm39)	missense	probably damaging	1.00
IGL00912:Vmn2r100	APN	17	19,751,654 (GRCm39)	missense	possibly damaging	0.95
IGL01107:Vmn2r100	APN	17	19,741,618 (GRCm39)	missense	probably damaging	1.00
IGL01517:Vmn2r100	APN	17	19,742,225 (GRCm39)	missense	probably benign	0.37
IGL01594:Vmn2r100	APN	17	19,751,495 (GRCm39)	missense	possibly damaging	0.52
IGL01657:Vmn2r100	APN	17	19,746,178 (GRCm39)	missense	possibly damaging	0.89
IGL01822:Vmn2r100	APN	17	19,725,100 (GRCm39)	missense	probably null	0.00
IGL02020:Vmn2r100	APN	17	19,725,200 (GRCm39)	missense	possibly damaging	0.78
IGL02060:Vmn2r100	APN	17	19,741,516 (GRCm39)	missense	possibly damaging	0.79
IGL02126:Vmn2r100	APN	17	19,741,504 (GRCm39)	splice site	probably benign
IGL02142:Vmn2r100	APN	17	19,742,583 (GRCm39)	missense	probably damaging	1.00
IGL02308:Vmn2r100	APN	17	19,741,597 (GRCm39)	missense	possibly damaging	0.90
IGL02407:Vmn2r100	APN	17	19,741,770 (GRCm39)	missense	probably damaging	0.98
IGL02469:Vmn2r100	APN	17	19,751,547 (GRCm39)	nonsense	probably null
IGL03088:Vmn2r100	APN	17	19,742,301 (GRCm39)	missense	probably benign	0.27
IGL03181:Vmn2r100	APN	17	19,752,207 (GRCm39)	missense	probably damaging	1.00
IGL03405:Vmn2r100	APN	17	19,752,186 (GRCm39)	missense	probably damaging	1.00
H8562:Vmn2r100	UTSW	17	19,741,752 (GRCm39)	missense	possibly damaging	0.87
R0012:Vmn2r100	UTSW	17	19,746,296 (GRCm39)	missense	probably damaging	0.99
R0012:Vmn2r100	UTSW	17	19,725,136 (GRCm39)	missense	probably benign
R0044:Vmn2r100	UTSW	17	19,742,441 (GRCm39)	missense	possibly damaging	0.46
R0109:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0111:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0112:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0149:Vmn2r100	UTSW	17	19,741,509 (GRCm39)	critical splice acceptor site	probably null
R0355:Vmn2r100	UTSW	17	19,751,582 (GRCm39)	missense	probably benign	0.00
R0395:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0396:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0453:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0465:Vmn2r100	UTSW	17	19,751,792 (GRCm39)	missense	probably damaging	0.98
R0477:Vmn2r100	UTSW	17	19,742,776 (GRCm39)	missense	probably benign	0.00
R0510:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0512:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0514:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0518:Vmn2r100	UTSW	17	19,742,178 (GRCm39)	missense	probably damaging	1.00
R0521:Vmn2r100	UTSW	17	19,742,178 (GRCm39)	missense	probably damaging	1.00
R0555:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0608:Vmn2r100	UTSW	17	19,742,382 (GRCm39)	missense	possibly damaging	0.80
R0959:Vmn2r100	UTSW	17	19,743,786 (GRCm39)	missense	possibly damaging	0.95
R1114:Vmn2r100	UTSW	17	19,752,261 (GRCm39)	missense	probably damaging	1.00
R2027:Vmn2r100	UTSW	17	19,742,334 (GRCm39)	missense	probably benign	0.02
R2049:Vmn2r100	UTSW	17	19,742,312 (GRCm39)	missense	probably benign	0.00
R2224:Vmn2r100	UTSW	17	19,742,634 (GRCm39)	missense	probably benign	0.03
R2226:Vmn2r100	UTSW	17	19,742,634 (GRCm39)	missense	probably benign	0.03
R3618:Vmn2r100	UTSW	17	19,743,692 (GRCm39)	missense	probably benign
R3715:Vmn2r100	UTSW	17	19,752,272 (GRCm39)	missense	probably damaging	0.99
R4120:Vmn2r100	UTSW	17	19,752,215 (GRCm39)	missense	probably damaging	1.00
R4152:Vmn2r100	UTSW	17	19,743,681 (GRCm39)	frame shift	probably null
R4153:Vmn2r100	UTSW	17	19,743,681 (GRCm39)	frame shift	probably null
R4154:Vmn2r100	UTSW	17	19,743,681 (GRCm39)	frame shift	probably null
R4200:Vmn2r100	UTSW	17	19,742,797 (GRCm39)	missense	probably benign	0.29
R4632:Vmn2r100	UTSW	17	19,752,216 (GRCm39)	missense	probably damaging	1.00
R4720:Vmn2r100	UTSW	17	19,742,788 (GRCm39)	missense	probably benign	0.02
R4761:Vmn2r100	UTSW	17	19,741,630 (GRCm39)	missense	possibly damaging	0.47
R4831:Vmn2r100	UTSW	17	19,741,672 (GRCm39)	missense	probably benign	0.28
R4951:Vmn2r100	UTSW	17	19,752,300 (GRCm39)	missense	probably benign	0.01
R5211:Vmn2r100	UTSW	17	19,746,257 (GRCm39)	missense	possibly damaging	0.67
R5657:Vmn2r100	UTSW	17	19,725,178 (GRCm39)	missense	probably benign	0.31
R5883:Vmn2r100	UTSW	17	19,743,786 (GRCm39)	missense	probably benign
R5912:Vmn2r100	UTSW	17	19,752,071 (GRCm39)	missense	probably damaging	0.99
R6141:Vmn2r100	UTSW	17	19,742,576 (GRCm39)	missense	probably benign	0.07
R6146:Vmn2r100	UTSW	17	19,742,522 (GRCm39)	missense	probably benign	0.04
R6500:Vmn2r100	UTSW	17	19,742,355 (GRCm39)	missense	probably damaging	1.00
R6575:Vmn2r100	UTSW	17	19,741,671 (GRCm39)	missense	probably benign	0.12
R6647:Vmn2r100	UTSW	17	19,742,785 (GRCm39)	missense	probably benign	0.00
R7038:Vmn2r100	UTSW	17	19,725,263 (GRCm39)	missense	possibly damaging	0.76
R7052:Vmn2r100	UTSW	17	19,751,556 (GRCm39)	missense	possibly damaging	0.95
R7170:Vmn2r100	UTSW	17	19,752,233 (GRCm39)	missense	probably benign	0.00
R7209:Vmn2r100	UTSW	17	19,751,576 (GRCm39)	missense	not run
R7312:Vmn2r100	UTSW	17	19,742,296 (GRCm39)	missense	probably benign	0.01
R7734:Vmn2r100	UTSW	17	19,742,296 (GRCm39)	missense	probably benign	0.01
R7750:Vmn2r100	UTSW	17	19,742,726 (GRCm39)	missense	probably benign
R8103:Vmn2r100	UTSW	17	19,751,415 (GRCm39)	splice site	probably null
R8193:Vmn2r100	UTSW	17	19,725,102 (GRCm39)	nonsense	probably null
R8267:Vmn2r100	UTSW	17	19,742,752 (GRCm39)	nonsense	probably null
R8290:Vmn2r100	UTSW	17	19,751,612 (GRCm39)	missense	probably damaging	0.99
R8531:Vmn2r100	UTSW	17	19,742,459 (GRCm39)	missense	possibly damaging	0.66
R8786:Vmn2r100	UTSW	17	19,742,838 (GRCm39)	missense	probably damaging	1.00
R8920:Vmn2r100	UTSW	17	19,741,620 (GRCm39)	missense	probably damaging	1.00
R8938:Vmn2r100	UTSW	17	19,751,825 (GRCm39)	missense	probably benign	0.00
R9555:Vmn2r100	UTSW	17	19,743,857 (GRCm39)	missense	probably benign	0.00
R9572:Vmn2r100	UTSW	17	19,741,513 (GRCm39)	missense	probably benign	0.00
R9609:Vmn2r100	UTSW	17	19,743,732 (GRCm39)	missense	probably damaging	1.00
R9618:Vmn2r100	UTSW	17	19,742,583 (GRCm39)	missense	probably damaging	1.00
X0062:Vmn2r100	UTSW	17	19,751,652 (GRCm39)	missense	possibly damaging	0.89
Z1176:Vmn2r100	UTSW	17	19,741,792 (GRCm39)	missense	probably benign	0.00
Z1177:Vmn2r100	UTSW	17	19,725,251 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GTGGCATTCTCAATCCCTTGG -3'
(R):5'- GCCTCTAAGTCATGAGAATTCAGAC -3'

Sequencing Primer
(F):5'- CTCAATCCCTTGGAATGATTAGTGTG -3'
(R):5'- ATTGTACGTACTGTATGTAATTGTCC -3'

Posted On

2016-10-24