Mutagenetix > Incidental Mutation

Incidental Mutation 'R5562:Zfp560'

436663

Institutional Source

Beutler Lab

Gene Symbol

Zfp560

Ensembl Gene

ENSMUSG00000045519

Gene Name

zinc finger protein 560

Synonyms

2310030G09Rik

MMRRC Submission

043119-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5562 (G1)

Quality Score

191

Status

Not validated

Chromosome

Chromosomal Location

20256432-20296473 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 20261883 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 89 (Y89*)

Ref Sequence

ENSEMBL: ENSMUSP00000065620 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068079] [ENSMUST00000143992]

AlphaFold

Q3URI6

Predicted Effect

probably null
Transcript: ENSMUST00000068079
AA Change: Y89*

SMART Domains

Protein: ENSMUSP00000065620
Gene: ENSMUSG00000045519
AA Change: Y89*

Domain	Start	End	E-Value	Type
KRAB	41	101	3.22e-27	SMART
low complexity region	147	158	N/A	INTRINSIC
ZnF_C2H2	279	301	4.01e-5	SMART
ZnF_C2H2	307	329	9.58e-3	SMART
ZnF_C2H2	335	357	5.5e-3	SMART
ZnF_C2H2	363	385	9.58e-3	SMART
ZnF_C2H2	391	413	3.74e-5	SMART
ZnF_C2H2	419	441	2.43e-4	SMART
ZnF_C2H2	447	469	1.28e-3	SMART
ZnF_C2H2	475	497	1.06e-4	SMART
ZnF_C2H2	503	525	3.11e-2	SMART
ZnF_C2H2	531	553	8.47e-4	SMART
ZnF_C2H2	559	581	2.99e-4	SMART
ZnF_C2H2	587	609	4.24e-4	SMART
ZnF_C2H2	615	637	3.44e-4	SMART
ZnF_C2H2	643	665	1.26e-2	SMART
ZnF_C2H2	671	693	1.69e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000143992

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214965

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldh1a7	G	A	19: 20,679,628 (GRCm39)	Q383*	probably null	Het
Alkbh3	A	T	2: 93,826,724 (GRCm39)		probably null	Het
Amotl1	G	A	9: 14,486,593 (GRCm39)	P434S	possibly damaging	Het
Arfgef1	T	C	1: 10,214,971 (GRCm39)	E1641G	probably damaging	Het
Arih2	T	C	9: 108,484,546 (GRCm39)	T422A	probably damaging	Het
C7	A	T	15: 5,061,397 (GRCm39)	Y317*	probably null	Het
Car4	A	T	11: 84,854,924 (GRCm39)	M91L	probably benign	Het
Ccdc7a	T	C	8: 129,785,266 (GRCm39)	D98G	possibly damaging	Het
Cdc25b	A	G	2: 131,036,678 (GRCm39)	M493V	probably damaging	Het
Cdhr3	C	G	12: 33,101,054 (GRCm39)	R452T	probably benign	Het
Col6a2	G	A	10: 76,435,509 (GRCm39)	Q909*	probably null	Het
Cyp2j8	A	G	4: 96,358,890 (GRCm39)	I343T	probably damaging	Het
Dcstamp	G	A	15: 39,617,798 (GRCm39)	C69Y	possibly damaging	Het
Dnaaf8	C	A	16: 4,791,940 (GRCm39)		noncoding transcript	Het
Efhc1	C	T	1: 21,043,104 (GRCm39)	T341I	probably damaging	Het
Elovl2	A	G	13: 41,338,772 (GRCm39)	*276Q	probably null	Het
Fnip1	T	A	11: 54,380,168 (GRCm39)		probably null	Het
Foxc1	A	G	13: 31,991,573 (GRCm39)	H128R	probably damaging	Het
Gpr107	C	T	2: 31,042,375 (GRCm39)	A2V	probably damaging	Het
Gprc5c	G	T	11: 114,755,093 (GRCm39)	V257L	possibly damaging	Het
Kif15	A	T	9: 122,807,081 (GRCm39)	Q44H	probably damaging	Het
Masp1	T	C	16: 23,283,917 (GRCm39)		probably null	Het
Muc5b	T	C	7: 141,400,975 (GRCm39)	I530T	unknown	Het
Ncoa4-ps	T	A	12: 119,225,957 (GRCm39)		noncoding transcript	Het
Nherf2	A	G	17: 24,860,798 (GRCm39)	V137A	probably benign	Het
Nudt7	C	A	8: 114,874,723 (GRCm39)	A93D	probably damaging	Het
Or5w1b	T	C	2: 87,476,063 (GRCm39)	I135V	probably benign	Het
Pcdha8	A	G	18: 37,126,024 (GRCm39)	T169A	possibly damaging	Het
Prnp	A	G	2: 131,778,951 (GRCm39)	D201G	probably damaging	Het
Serinc1	G	A	10: 57,400,147 (GRCm39)	Q167*	probably null	Het
Slc13a5	A	G	11: 72,152,865 (GRCm39)	V35A	probably damaging	Het
Slc30a6	C	T	17: 74,719,700 (GRCm39)	T220I	possibly damaging	Het
Slc7a7	T	A	14: 54,646,269 (GRCm39)	M65L	probably benign	Het
Speg	T	A	1: 75,403,700 (GRCm39)	L2627Q	probably damaging	Het
Tank	A	G	2: 61,480,552 (GRCm39)	T363A	possibly damaging	Het
Taok3	T	A	5: 117,389,029 (GRCm39)	L478Q	probably damaging	Het
Trim55	A	T	3: 19,713,317 (GRCm39)	M123L	probably benign	Het
Trpm2	A	G	10: 77,795,773 (GRCm39)	V118A	possibly damaging	Het
Ttn	A	T	2: 76,600,803 (GRCm39)	Y17114N	probably damaging	Het
Unc5c	A	G	3: 141,474,291 (GRCm39)	T214A	probably damaging	Het
Ush2a	T	A	1: 188,308,414 (GRCm39)	V2021E	probably damaging	Het
Utp4	G	A	8: 107,649,557 (GRCm39)	D669N	probably benign	Het
Zfp64	A	G	2: 168,767,642 (GRCm39)	S657P	probably benign	Het

Other mutations in Zfp560

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00933:Zfp560	APN	9	20,260,104 (GRCm39)	missense	probably benign	0.00
IGL02400:Zfp560	APN	9	20,261,896 (GRCm39)	missense	possibly damaging	0.73
R0002:Zfp560	UTSW	9	20,258,813 (GRCm39)	missense	probably damaging	1.00
R0004:Zfp560	UTSW	9	20,259,263 (GRCm39)	missense	probably damaging	1.00
R0019:Zfp560	UTSW	9	20,259,656 (GRCm39)	missense	probably benign	0.23
R1401:Zfp560	UTSW	9	20,263,149 (GRCm39)	missense	possibly damaging	0.71
R1481:Zfp560	UTSW	9	20,260,086 (GRCm39)	missense	probably benign
R1521:Zfp560	UTSW	9	20,260,071 (GRCm39)	splice site	probably null
R1569:Zfp560	UTSW	9	20,260,011 (GRCm39)	missense	possibly damaging	0.83
R1579:Zfp560	UTSW	9	20,259,287 (GRCm39)	missense	possibly damaging	0.73
R1673:Zfp560	UTSW	9	20,258,949 (GRCm39)	missense	probably benign	0.37
R1694:Zfp560	UTSW	9	20,259,282 (GRCm39)	nonsense	probably null
R1796:Zfp560	UTSW	9	20,263,226 (GRCm39)	missense	possibly damaging	0.71
R2971:Zfp560	UTSW	9	20,260,240 (GRCm39)	missense	probably benign	0.00
R3416:Zfp560	UTSW	9	20,258,974 (GRCm39)	nonsense	probably null
R4182:Zfp560	UTSW	9	20,258,744 (GRCm39)	missense	probably benign	0.11
R4509:Zfp560	UTSW	9	20,260,019 (GRCm39)	missense	probably damaging	1.00
R4708:Zfp560	UTSW	9	20,263,214 (GRCm39)	missense	possibly damaging	0.85
R4735:Zfp560	UTSW	9	20,260,347 (GRCm39)	missense	probably benign	0.01
R4937:Zfp560	UTSW	9	20,259,263 (GRCm39)	missense	probably damaging	1.00
R6597:Zfp560	UTSW	9	20,259,297 (GRCm39)	missense	probably benign	0.00
R6852:Zfp560	UTSW	9	20,259,339 (GRCm39)	missense	probably damaging	0.99
R6863:Zfp560	UTSW	9	20,259,795 (GRCm39)	missense	probably damaging	0.99
R7267:Zfp560	UTSW	9	20,259,384 (GRCm39)	missense	probably damaging	0.96
R7619:Zfp560	UTSW	9	20,260,206 (GRCm39)	missense	probably benign	0.01
R7763:Zfp560	UTSW	9	20,258,619 (GRCm39)	missense	possibly damaging	0.96
R8220:Zfp560	UTSW	9	20,260,348 (GRCm39)	missense	probably benign	0.00
R8356:Zfp560	UTSW	9	20,260,231 (GRCm39)	missense	probably benign	0.25
R8858:Zfp560	UTSW	9	20,260,403 (GRCm39)	missense	probably benign	0.12
R8992:Zfp560	UTSW	9	20,260,895 (GRCm39)	missense	probably benign
Z1176:Zfp560	UTSW	9	20,259,000 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GTCTCAGCCTTCTGACTCAAG -3'
(R):5'- TATGAAGAAGTCAGTACCTACCAAC -3'

Sequencing Primer
(F):5'- CAGCCTTCTGACTCAAGTTTTC -3'
(R):5'- GTACCTACCAACACATATAAGGTCTG -3'

Posted On

2016-10-24