Incidental Mutation 'R5566:Wee1'
ID 436820
Institutional Source Beutler Lab
Gene Symbol Wee1
Ensembl Gene ENSMUSG00000031016
Gene Name WEE 1 homolog 1 (S. pombe)
Synonyms Wee1A
MMRRC Submission 043123-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R5566 (G1)
Quality Score 225
Status Not validated
Chromosome 7
Chromosomal Location 109721266-109742506 bp(+) (GRCm39)
Type of Mutation nonsense
DNA Base Change (assembly) G to T at 109725257 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Stop codon at position 300 (E300*)
Ref Sequence ENSEMBL: ENSMUSP00000033326 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000033326]
AlphaFold P47810
Predicted Effect probably null
Transcript: ENSMUST00000033326
AA Change: E300*
SMART Domains Protein: ENSMUSP00000033326
Gene: ENSMUSG00000031016
AA Change: E300*

DomainStartEndE-ValueType
low complexity region 62 112 N/A INTRINSIC
Pfam:Pkinase_Tyr 298 566 1.9e-26 PFAM
Pfam:Pkinase 298 568 1.7e-61 PFAM
low complexity region 620 631 N/A INTRINSIC
Predicted Effect noncoding transcript
Transcript: ENSMUST00000185931
Predicted Effect noncoding transcript
Transcript: ENSMUST00000210940
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.4%
  • 20x: 95.5%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 97 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 T A 11: 9,244,615 (GRCm39) Y2159* probably null Het
Abca3 A G 17: 24,602,901 (GRCm39) T499A probably benign Het
Abcb5 T A 12: 118,899,702 (GRCm39) T322S probably damaging Het
Adamts4 T A 1: 171,078,419 (GRCm39) M1K probably null Het
Adamtsl4 C T 3: 95,592,765 (GRCm39) probably null Het
Adh4 A T 3: 138,129,950 (GRCm39) I259F probably damaging Het
Aff3 G A 1: 38,220,505 (GRCm39) S1135F probably damaging Het
Arhgef16 T C 4: 154,370,105 (GRCm39) D280G probably benign Het
Baiap3 T C 17: 25,470,707 (GRCm39) E71G probably damaging Het
Calb2 A G 8: 110,879,332 (GRCm39) I91T possibly damaging Het
Ccr5 A G 9: 123,924,697 (GRCm39) N100S probably benign Het
Cep57 G T 9: 13,732,871 (GRCm39) R25S probably damaging Het
Chadl A G 15: 81,580,079 (GRCm39) L52P probably damaging Het
Clec12b T C 6: 129,362,438 (GRCm39) T6A probably damaging Het
Cnga1 T A 5: 72,775,593 (GRCm39) N43Y probably damaging Het
Col20a1 A T 2: 180,628,316 (GRCm39) probably null Het
Csmd2 T C 4: 128,356,682 (GRCm39) probably null Het
Ctps1 A T 4: 120,411,300 (GRCm39) probably null Het
Cyp39a1 T A 17: 43,996,099 (GRCm39) W224R possibly damaging Het
Defb29 A T 2: 152,380,848 (GRCm39) Y54N probably benign Het
Dmbt1 A T 7: 130,708,003 (GRCm39) D1241V probably damaging Het
Dnah1 A T 14: 30,996,323 (GRCm39) I2671K probably benign Het
Dnah2 C A 11: 69,407,395 (GRCm39) E158* probably null Het
Dynlt2b A G 16: 32,238,718 (GRCm39) Y31C probably damaging Het
Edar A G 10: 58,464,463 (GRCm39) S59P possibly damaging Het
Egr2 T A 10: 67,376,596 (GRCm39) C339* probably null Het
Eif5b G A 1: 38,084,765 (GRCm39) V871I possibly damaging Het
Eif5b G A 1: 38,090,328 (GRCm39) G1169E probably damaging Het
Erap1 A G 13: 74,810,531 (GRCm39) Y290C probably damaging Het
Fastkd5 T A 2: 130,456,221 (GRCm39) T790S possibly damaging Het
Fkrp C T 7: 16,544,849 (GRCm39) V338M probably damaging Het
Gabra6 T C 11: 42,198,317 (GRCm39) T378A probably benign Het
Gm4924 C A 10: 82,214,475 (GRCm39) Q17K possibly damaging Het
Gm9847 A G 12: 14,545,000 (GRCm39) noncoding transcript Het
Gpr108 A G 17: 57,543,919 (GRCm39) F429S probably damaging Het
Gpr162 G A 6: 124,837,901 (GRCm39) R250* probably null Het
Gtf2a1 A T 12: 91,534,368 (GRCm39) D295E possibly damaging Het
Gtf2h3 C T 5: 124,722,360 (GRCm39) T121I probably benign Het
Herc1 T A 9: 66,372,819 (GRCm39) M3125K possibly damaging Het
Hoxb6 T A 11: 96,191,580 (GRCm39) Y167* probably null Het
Hrob T A 11: 102,146,659 (GRCm39) S312T probably damaging Het
Htt T C 5: 35,006,419 (GRCm39) Y1443H probably damaging Het
Il21r A G 7: 125,224,470 (GRCm39) D28G probably damaging Het
Impact T G 18: 13,107,819 (GRCm39) V29G probably damaging Het
Itpr3 A G 17: 27,334,926 (GRCm39) T2147A possibly damaging Het
Itsn2 T A 12: 4,676,554 (GRCm39) L50Q probably damaging Het
Jade1 A G 3: 41,559,338 (GRCm39) D473G possibly damaging Het
Kif26a T G 12: 112,123,788 (GRCm39) L131R probably damaging Het
Kif2a A T 13: 107,130,432 (GRCm39) M1K probably null Het
Lrsam1 C A 2: 32,831,870 (GRCm39) Q368H probably damaging Het
Macf1 T C 4: 123,328,957 (GRCm39) Q4592R probably damaging Het
Map3k5 T C 10: 19,986,465 (GRCm39) V893A probably damaging Het
Med13l G T 5: 118,866,730 (GRCm39) V595F possibly damaging Het
Mocs1 T A 17: 49,761,211 (GRCm39) L435Q possibly damaging Het
Mtmr4 T C 11: 87,495,356 (GRCm39) L471P probably damaging Het
Myo7a T C 7: 97,714,023 (GRCm39) E1616G possibly damaging Het
Nacad T A 11: 6,552,136 (GRCm39) S352C probably damaging Het
Nfat5 T A 8: 108,095,767 (GRCm39) M817K possibly damaging Het
Ofcc1 C A 13: 40,248,129 (GRCm39) L668F probably damaging Het
Or12j2 C A 7: 139,915,980 (GRCm39) D68E probably damaging Het
Or7e178 T C 9: 20,225,265 (GRCm39) Q309R probably benign Het
Or8j3c G A 2: 86,253,721 (GRCm39) Q100* probably null Het
Plxnb2 T C 15: 89,048,223 (GRCm39) T696A probably benign Het
Prkab2 T A 3: 97,569,609 (GRCm39) F58L probably benign Het
Prpf4 G T 4: 62,334,206 (GRCm39) L220F probably benign Het
Rad18 A T 6: 112,658,307 (GRCm39) D199E probably benign Het
Raet1e T C 10: 22,050,304 (GRCm39) L29P probably damaging Het
Ralgapb A C 2: 158,336,630 (GRCm39) T1089P possibly damaging Het
Rest A G 5: 77,430,173 (GRCm39) E864G probably benign Het
Rgsl1 T A 1: 153,669,520 (GRCm39) I289F probably damaging Het
Rint1 T C 5: 24,015,951 (GRCm39) Y406H probably damaging Het
Rmc1 T C 18: 12,313,749 (GRCm39) I26T possibly damaging Het
Rpl31 C T 1: 39,409,108 (GRCm39) R41C probably benign Het
Scn8a T C 15: 100,872,415 (GRCm39) S485P probably damaging Het
Slamf1 T A 1: 171,615,538 (GRCm39) V249E possibly damaging Het
Slc39a12 C T 2: 14,412,414 (GRCm39) T362I possibly damaging Het
Sos1 C T 17: 80,761,319 (GRCm39) V126I possibly damaging Het
Srcap T C 7: 127,124,475 (GRCm39) F215S probably damaging Het
Supt4a T A 11: 87,634,113 (GRCm39) S110T probably benign Het
Tbc1d30 T A 10: 121,138,015 (GRCm39) T232S probably damaging Het
Tenm3 A G 8: 48,732,041 (GRCm39) C1288R probably damaging Het
Tespa1 T A 10: 130,191,356 (GRCm39) L100* probably null Het
Tgm1 C A 14: 55,949,893 (GRCm39) R105L probably damaging Het
Tgoln1 G A 6: 72,593,018 (GRCm39) T154I possibly damaging Het
Trp53i13 G A 11: 77,399,552 (GRCm39) T259I probably damaging Het
Tubgcp4 T A 2: 121,015,251 (GRCm39) F320I possibly damaging Het
Tut7 A T 13: 59,936,443 (GRCm39) C817* probably null Het
Vmn1r21 A C 6: 57,821,079 (GRCm39) Y122D probably benign Het
Vmn1r75 T A 7: 11,614,407 (GRCm39) D46E probably damaging Het
Vmn2r120 A T 17: 57,852,290 (GRCm39) L9M possibly damaging Het
Vmn2r59 A G 7: 41,696,247 (GRCm39) I165T possibly damaging Het
Vmn2r76 A T 7: 85,875,286 (GRCm39) Y564N probably damaging Het
Xdh T C 17: 74,200,617 (GRCm39) D1168G probably damaging Het
Zfp54 A G 17: 21,653,706 (GRCm39) T67A probably damaging Het
Zfp941 G T 7: 140,392,679 (GRCm39) H227N probably benign Het
Zpr1 T A 9: 46,192,373 (GRCm39) V399D possibly damaging Het
Zzz3 A G 3: 152,161,461 (GRCm39) E285G probably damaging Het
Other mutations in Wee1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00693:Wee1 APN 7 109,734,060 (GRCm39) splice site probably null
IGL00981:Wee1 APN 7 109,738,876 (GRCm39) missense probably damaging 1.00
IGL01017:Wee1 APN 7 109,725,055 (GRCm39) missense possibly damaging 0.93
IGL01357:Wee1 APN 7 109,741,242 (GRCm39) missense probably benign 0.39
IGL01838:Wee1 APN 7 109,723,744 (GRCm39) missense probably benign 0.01
IGL01970:Wee1 APN 7 109,738,457 (GRCm39) missense probably damaging 1.00
IGL02396:Wee1 APN 7 109,741,300 (GRCm39) missense probably damaging 1.00
IGL02511:Wee1 APN 7 109,738,483 (GRCm39) missense possibly damaging 0.55
IGL02884:Wee1 APN 7 109,725,269 (GRCm39) missense probably benign 0.02
IGL03085:Wee1 APN 7 109,723,805 (GRCm39) missense probably damaging 1.00
IGL03221:Wee1 APN 7 109,726,024 (GRCm39) missense probably damaging 1.00
IGL03383:Wee1 APN 7 109,738,899 (GRCm39) missense probably damaging 1.00
R0220:Wee1 UTSW 7 109,723,733 (GRCm39) missense probably benign 0.10
R1934:Wee1 UTSW 7 109,721,698 (GRCm39) missense probably benign 0.06
R3110:Wee1 UTSW 7 109,730,043 (GRCm39) missense probably damaging 1.00
R3112:Wee1 UTSW 7 109,730,043 (GRCm39) missense probably damaging 1.00
R3978:Wee1 UTSW 7 109,723,762 (GRCm39) missense probably damaging 1.00
R4348:Wee1 UTSW 7 109,730,165 (GRCm39) missense probably damaging 1.00
R5434:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5435:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5436:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5449:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5630:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5632:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5685:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5694:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5807:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R5941:Wee1 UTSW 7 109,723,776 (GRCm39) frame shift probably null
R6044:Wee1 UTSW 7 109,738,513 (GRCm39) missense probably benign 0.00
R6163:Wee1 UTSW 7 109,734,858 (GRCm39) missense probably damaging 1.00
R6826:Wee1 UTSW 7 109,723,870 (GRCm39) critical splice donor site probably null
R7203:Wee1 UTSW 7 109,734,001 (GRCm39) missense probably benign 0.00
R7835:Wee1 UTSW 7 109,730,085 (GRCm39) nonsense probably null
R8273:Wee1 UTSW 7 109,723,691 (GRCm39) missense probably benign 0.00
R8953:Wee1 UTSW 7 109,723,691 (GRCm39) missense probably benign 0.00
R9077:Wee1 UTSW 7 109,725,963 (GRCm39) missense probably damaging 1.00
R9336:Wee1 UTSW 7 109,721,689 (GRCm39) missense probably damaging 1.00
R9463:Wee1 UTSW 7 109,721,917 (GRCm39) missense probably damaging 1.00
R9673:Wee1 UTSW 7 109,725,210 (GRCm39) missense probably damaging 0.98
R9748:Wee1 UTSW 7 109,721,722 (GRCm39) nonsense probably null
Predicted Primers PCR Primer
(F):5'- AGTTAGTAGGTAAGGTCTCAAGTTG -3'
(R):5'- AAAAGCATTAGGTATTTGGTTACCC -3'

Sequencing Primer
(F):5'- GTTCCTCTGAAGACATGGAAGCC -3'
(R):5'- GAGCCAGCCAATGGTTTT -3'
Posted On 2016-10-24