Mutagenetix > Incidental Mutation

Incidental Mutation 'R5568:Tln2'

437037

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

043125-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.207) question?

Stock #

R5568 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67124369-67466985 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 67219147 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 266 (I266T)

Ref Sequence

ENSEMBL: ENSMUSP00000149474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215267] [ENSMUST00000215784] [ENSMUST00000217550]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000039662
AA Change: I1352T

PolyPhen 2 Score 0.328 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: I1352T

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000040025
AA Change: I1352T

PolyPhen 2 Score 0.328 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: I1352T

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000215267
AA Change: I262T

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215784
AA Change: I1354T

PolyPhen 2 Score 0.457 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

probably damaging
Transcript: ENSMUST00000217550
AA Change: I266T

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4	T	C	4: 144,349,364 (GRCm39)	V207A	probably benign	Het
Abcc10	C	A	17: 46,614,834 (GRCm39)		probably null	Het
Abcc9	A	C	6: 142,634,742 (GRCm39)	V174G	possibly damaging	Het
Abl1	C	T	2: 31,669,086 (GRCm39)	A155V	probably damaging	Het
Aco2	T	A	15: 81,787,787 (GRCm39)	D212E	probably damaging	Het
Adam26b	A	T	8: 43,973,529 (GRCm39)	M491K	probably benign	Het
Anapc5	G	A	5: 122,929,988 (GRCm39)		probably benign	Het
Atf7	A	G	15: 102,471,757 (GRCm39)	I46T	probably damaging	Het
Cacna1b	A	G	2: 24,497,612 (GRCm39)	S2100P	probably damaging	Het
Capn5	T	A	7: 97,775,137 (GRCm39)	D501V	probably damaging	Het
Cc2d2a	A	G	5: 43,866,433 (GRCm39)	M748V	probably damaging	Het
Cd300c2	T	C	11: 114,891,662 (GRCm39)	T71A	probably damaging	Het
Chmp2a	T	C	7: 12,767,758 (GRCm39)	M56V	probably benign	Het
Cilp	A	C	9: 65,187,515 (GRCm39)	R1203S	probably benign	Het
Clp1	T	A	2: 84,556,322 (GRCm39)	K53*	probably null	Het
Crhbp	T	A	13: 95,578,737 (GRCm39)	D128V	probably damaging	Het
Crispld1	T	A	1: 17,820,495 (GRCm39)	I292N	probably benign	Het
Cyp2c68	A	T	19: 39,677,526 (GRCm39)	I488N	probably benign	Het
Cyp3a57	A	T	5: 145,307,456 (GRCm39)	M149L	probably benign	Het
Ddx24	T	A	12: 103,390,547 (GRCm39)	Q59L	possibly damaging	Het
Ddx27	A	G	2: 166,871,439 (GRCm39)	H512R	possibly damaging	Het
Dlgap4	T	A	2: 156,604,821 (GRCm39)	*993K	probably null	Het
Dmxl2	A	T	9: 54,330,643 (GRCm39)		probably null	Het
Dus4l	A	T	12: 31,696,712 (GRCm39)	F88L	probably damaging	Het
Ep400	A	T	5: 110,904,071 (GRCm39)	V176E	probably damaging	Het
Fat3	T	A	9: 16,288,219 (GRCm39)	K435*	probably null	Het
Fsip2	T	C	2: 82,816,908 (GRCm39)	C4214R	probably benign	Het
Garin5a	T	G	7: 44,150,428 (GRCm39)	S207A	probably damaging	Het
Gfral	T	C	9: 76,072,087 (GRCm39)	*394W	probably null	Het
Glis1	T	C	4: 107,476,832 (GRCm39)	S518P	probably damaging	Het
H2-T10	A	G	17: 36,430,079 (GRCm39)		probably null	Het
Hsbp1l1	T	C	18: 80,278,679 (GRCm39)	T35A	possibly damaging	Het
Ighv5-12	A	G	12: 113,665,837 (GRCm39)	F87S	probably damaging	Het
Ints13	A	C	6: 146,477,855 (GRCm39)	D31E	probably damaging	Het
Kbtbd12	C	T	6: 88,595,609 (GRCm39)	D74N	probably damaging	Het
Klrb1c	A	G	6: 128,765,877 (GRCm39)		probably benign	Het
Kmt5b	A	T	19: 3,836,538 (GRCm39)	H25L	probably benign	Het
Krt28	A	T	11: 99,262,210 (GRCm39)	M260K	probably damaging	Het
Krt79	A	G	15: 101,838,220 (GRCm39)	S512P	probably damaging	Het
Lama1	A	T	17: 68,075,293 (GRCm39)		probably null	Het
Maneal	T	C	4: 124,750,937 (GRCm39)	E273G	possibly damaging	Het
Map4k3	T	A	17: 80,971,427 (GRCm39)	Y80F	possibly damaging	Het
Mbd6	A	G	10: 127,119,297 (GRCm39)	V946A	possibly damaging	Het
Mfsd14b	A	T	13: 65,219,936 (GRCm39)		probably null	Het
Mrpl46	C	G	7: 78,430,242 (GRCm39)	W176S	probably damaging	Het
Muc19	A	G	15: 91,768,468 (GRCm39)		noncoding transcript	Het
Mup3	T	G	4: 62,002,809 (GRCm39)	E184A	possibly damaging	Het
Myo9a	A	G	9: 59,781,911 (GRCm39)	H1699R	probably benign	Het
Ndrg2	T	A	14: 52,144,420 (GRCm39)	T269S	probably damaging	Het
Nfatc1	A	T	18: 80,693,037 (GRCm39)	V688D	probably benign	Het
Ninj2	T	C	6: 120,175,670 (GRCm39)	I101T	probably benign	Het
Nlrp10	T	A	7: 108,523,468 (GRCm39)	M671L	probably benign	Het
Npsr1	T	A	9: 24,224,510 (GRCm39)	L296I	probably damaging	Het
Or10d5	A	G	9: 39,861,983 (GRCm39)	L28P	probably benign	Het
Or52r1b	G	T	7: 102,691,517 (GRCm39)	R272L	possibly damaging	Het
Pacsin1	T	A	17: 27,927,022 (GRCm39)	D242E	probably damaging	Het
Pcdh1	T	C	18: 38,330,420 (GRCm39)	Y861C	probably damaging	Het
Pcdha12	T	C	18: 37,153,443 (GRCm39)	L54P	probably damaging	Het
Pcdhb18	T	A	18: 37,624,853 (GRCm39)	S728T	probably benign	Het
Phyhd1	T	A	2: 30,167,022 (GRCm39)	H108Q	probably damaging	Het
Plcb1	A	T	2: 135,212,513 (GRCm39)	I1035F	probably damaging	Het
Plcl1	T	C	1: 55,735,309 (GRCm39)	S217P	possibly damaging	Het
Plppr2	G	A	9: 21,852,425 (GRCm39)	R103H	probably damaging	Het
Plxnb2	G	A	15: 89,041,638 (GRCm39)	T1722I	probably damaging	Het
Pole3	T	C	4: 62,442,668 (GRCm39)	N53S	probably damaging	Het
Ptk6	A	T	2: 180,841,488 (GRCm39)	N140K	possibly damaging	Het
Rab12	C	T	17: 66,804,418 (GRCm39)	R180H	probably damaging	Het
Rab36	G	T	10: 74,888,311 (GRCm39)	V252L	probably benign	Het
Ranbp3	T	C	17: 57,008,543 (GRCm39)		probably null	Het
Rapgef2	A	G	3: 79,011,308 (GRCm39)	L259P	probably damaging	Het
Rigi	T	A	4: 40,222,140 (GRCm39)	M380L	probably benign	Het
Scaf4	GGCTGCTGCTGCTGCTGCTGCTGCTG	GGCTGCTGCTGCTGCTGCTGCTG	16: 90,026,745 (GRCm39)		probably benign	Het
Scd2	T	C	19: 44,288,142 (GRCm39)	F178S	probably damaging	Het
Shmt2	A	G	10: 127,356,250 (GRCm39)	S87P	probably damaging	Het
Slf1	T	A	13: 77,194,823 (GRCm39)	D834V	probably damaging	Het
Sorcs2	A	C	5: 36,203,874 (GRCm39)	Y540*	probably null	Het
Srpk2	T	A	5: 23,730,697 (GRCm39)	Q274L	possibly damaging	Het
Stradb	T	A	1: 59,031,901 (GRCm39)	M271K	possibly damaging	Het
Tfec	T	A	6: 16,867,592 (GRCm39)	Q16L	possibly damaging	Het
Tfg	T	C	16: 56,521,450 (GRCm39)	T63A	probably benign	Het
Ticrr	G	A	7: 79,339,715 (GRCm39)		probably null	Het
Ticrr	T	A	7: 79,345,044 (GRCm39)	C1636*	probably null	Het
Tmcc1	G	C	6: 115,999,071 (GRCm39)	R323G	possibly damaging	Het
Tnnt3	A	G	7: 142,065,777 (GRCm39)	E138G	probably damaging	Het
Tpm2	C	A	4: 43,522,692 (GRCm39)	E75*	probably null	Het
Ttn	T	G	2: 76,580,922 (GRCm39)	T23324P	probably damaging	Het
Ubr4	T	G	4: 139,119,349 (GRCm39)	L176R	probably damaging	Het
Uhrf2	G	T	19: 30,016,488 (GRCm39)	D46Y	probably damaging	Het
Ulbp1	A	C	10: 7,423,281 (GRCm39)	S21A	unknown	Het
Usp17lb	C	T	7: 104,490,415 (GRCm39)	G170R	probably damaging	Het
Utp15	T	C	13: 98,394,433 (GRCm39)	N153S	probably benign	Het
Vcan	T	A	13: 89,836,790 (GRCm39)	E2918V	probably damaging	Het
Vmn1r174	T	A	7: 23,453,919 (GRCm39)	I195K	probably damaging	Het
Vmn1r76	C	T	7: 11,665,062 (GRCm39)	V16I	probably benign	Het
Xdh	T	C	17: 74,250,880 (GRCm39)	D24G	possibly damaging	Het
Xylb	T	A	9: 119,190,198 (GRCm39)	H68Q	probably benign	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,251,469 (GRCm39)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,157,864 (GRCm39)	nonsense	probably null
IGL01112:Tln2	APN	9	67,219,093 (GRCm39)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,302,749 (GRCm39)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,169,205 (GRCm39)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,277,905 (GRCm39)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,157,896 (GRCm39)	nonsense	probably null
IGL01999:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,263,980 (GRCm39)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,268,721 (GRCm39)	splice site	probably benign
IGL02368:Tln2	APN	9	67,148,092 (GRCm39)	splice site	probably benign
IGL02444:Tln2	APN	9	67,165,874 (GRCm39)	splice site	probably benign
IGL02646:Tln2	APN	9	67,163,278 (GRCm39)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,136,658 (GRCm39)	nonsense	probably null
IGL02869:Tln2	APN	9	67,128,807 (GRCm39)	splice site	probably benign
IGL02930:Tln2	APN	9	67,300,944 (GRCm39)	nonsense	probably null
IGL03100:Tln2	APN	9	67,203,019 (GRCm39)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,241,539 (GRCm39)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
Marsh	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0107:Tln2	UTSW	9	67,277,988 (GRCm39)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,262,479 (GRCm39)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,278,015 (GRCm39)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,203,095 (GRCm39)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,136,736 (GRCm39)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,219,121 (GRCm39)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,179,950 (GRCm39)	missense	possibly damaging	0.95
R1584:Tln2	UTSW	9	67,203,696 (GRCm39)	missense	probably damaging	1.00
R1636:Tln2	UTSW	9	67,213,814 (GRCm39)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,134,389 (GRCm39)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,283,089 (GRCm39)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,136,705 (GRCm39)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,249,325 (GRCm39)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,269,969 (GRCm39)	nonsense	probably null
R1964:Tln2	UTSW	9	67,249,417 (GRCm39)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,163,183 (GRCm39)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,179,986 (GRCm39)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,283,039 (GRCm39)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,262,421 (GRCm39)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,237,829 (GRCm39)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,163,197 (GRCm39)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,277,911 (GRCm39)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,251,347 (GRCm39)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,209,854 (GRCm39)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,293,837 (GRCm39)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,262,407 (GRCm39)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,261,750 (GRCm39)	missense	probably benign
R5092:Tln2	UTSW	9	67,163,310 (GRCm39)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,241,596 (GRCm39)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,261,764 (GRCm39)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,273,205 (GRCm39)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,149,641 (GRCm39)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,241,602 (GRCm39)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,203,640 (GRCm39)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,299,829 (GRCm39)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,165,532 (GRCm39)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,293,887 (GRCm39)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,174,150 (GRCm39)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,136,685 (GRCm39)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,269,999 (GRCm39)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,230,302 (GRCm39)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,131,363 (GRCm39)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,185,411 (GRCm39)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,179,947 (GRCm39)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,179,971 (GRCm39)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,293,946 (GRCm39)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,170,187 (GRCm39)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,193,840 (GRCm39)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,304,917 (GRCm39)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,299,814 (GRCm39)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,165,767 (GRCm39)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,269,929 (GRCm39)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,253,699 (GRCm39)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,170,261 (GRCm39)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,253,743 (GRCm39)	missense	probably benign
R7753:Tln2	UTSW	9	67,302,755 (GRCm39)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,255,508 (GRCm39)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,131,346 (GRCm39)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,264,029 (GRCm39)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,226,702 (GRCm39)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,253,811 (GRCm39)	nonsense	probably null
R8495:Tln2	UTSW	9	67,261,749 (GRCm39)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,165,555 (GRCm39)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,230,304 (GRCm39)	missense	probably benign
R8781:Tln2	UTSW	9	67,163,233 (GRCm39)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,128,799 (GRCm39)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,304,975 (GRCm39)	splice site	probably benign
R8837:Tln2	UTSW	9	67,157,866 (GRCm39)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,302,827 (GRCm39)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
R8867:Tln2	UTSW	9	67,237,832 (GRCm39)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,174,105 (GRCm39)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,253,843 (GRCm39)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,269,927 (GRCm39)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,128,778 (GRCm39)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,277,980 (GRCm39)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,229,213 (GRCm39)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,230,353 (GRCm39)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,262,529 (GRCm39)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,278,043 (GRCm39)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,273,249 (GRCm39)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,136,732 (GRCm39)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,299,769 (GRCm39)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,165,766 (GRCm39)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,157,826 (GRCm39)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,293,938 (GRCm39)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,284,135 (GRCm39)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,255,420 (GRCm39)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,277,973 (GRCm39)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,253,767 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TCCTTCAACCTAGGAGAGAGG -3'
(R):5'- CAATGGGAGGATAGACCCTTGG -3'

Sequencing Primer
(F):5'- CTTCAACCTAGGAGAGAGGCAAGC -3'
(R):5'- ACCCTTGGTCCTGTGAAGG -3'

Posted On

2016-10-24