Mutagenetix > Incidental Mutation

Incidental Mutation 'R0046:Olfr742'

43731

Institutional Source

Beutler Lab

Gene Symbol

Olfr742

Ensembl Gene

ENSMUSG00000068431

Gene Name

olfactory receptor 742

Synonyms

GA_x6K02T2PMLR-6224293-6225228, MOR106-6

MMRRC Submission

038340-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R0046 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

50503600-50517145 bp(+) (GRCm38)

Type of Mutation

makesense

DNA Base Change (assembly)

T to A at 50516139 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Lysine at position 312 (*312K)

Ref Sequence

ENSEMBL: ENSMUSP00000150814 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089836] [ENSMUST00000213935] [ENSMUST00000217437]

AlphaFold

A2RTP5

Predicted Effect

probably null
Transcript: ENSMUST00000089836
AA Change: *312K

SMART Domains

Protein: ENSMUSP00000087273
Gene: ENSMUSG00000068431
AA Change: *312K

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	311	1.9e-55	PFAM
Pfam:7tm_1	45	294	1.5e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000213935
AA Change: *312K

Predicted Effect

probably null
Transcript: ENSMUST00000217437
AA Change: *312K

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.6%
3x: 98.8%
10x: 96.7%
20x: 93.3%

Validation Efficiency

100% (83/83)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 81 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl7a	A	T	4: 56,743,877 (GRCm38)	K135*	probably null	Het
Adamts16	A	G	13: 70,763,460 (GRCm38)	S871P	probably benign	Het
Adcy10	A	T	1: 165,539,834 (GRCm38)	I558F	probably damaging	Het
Adsl	T	G	15: 80,962,788 (GRCm38)		probably null	Het
Aldob	T	C	4: 49,543,842 (GRCm38)	I47V	possibly damaging	Het
Alkbh8	A	G	9: 3,343,247 (GRCm38)	E46G	probably damaging	Het
Ankrd33b	G	A	15: 31,367,337 (GRCm38)	P19L	probably damaging	Het
Apoa5	T	C	9: 46,269,998 (GRCm38)	L124S	probably damaging	Het
Atp1a4	A	T	1: 172,240,097 (GRCm38)	L533Q	probably benign	Het
Atp7b	T	C	8: 22,059,995 (GRCm38)	T9A	probably benign	Het
Auh	G	A	13: 52,929,385 (GRCm38)		probably benign	Het
B3gnt3	T	C	8: 71,692,923 (GRCm38)	Y267C	probably damaging	Het
BC051142	T	C	17: 34,460,121 (GRCm38)		probably null	Het
Card11	T	C	5: 140,908,524 (GRCm38)	T117A	possibly damaging	Het
Ccdc39	A	G	3: 33,844,152 (GRCm38)	F15L	possibly damaging	Het
Chtf18	C	T	17: 25,723,460 (GRCm38)	R468Q	probably benign	Het
Cntnap5c	T	G	17: 58,359,300 (GRCm38)	D1108E	probably benign	Het
Col14a1	G	A	15: 55,408,963 (GRCm38)		probably benign	Het
Col6a6	C	T	9: 105,748,848 (GRCm38)		probably benign	Het
Col9a3	G	A	2: 180,609,487 (GRCm38)	A317T	possibly damaging	Het
Cpt1c	A	T	7: 44,959,832 (GRCm38)		probably benign	Het
Cpt2	A	G	4: 107,904,362 (GRCm38)		probably null	Het
Crebrf	T	A	17: 26,763,334 (GRCm38)	L565M	probably damaging	Het
Cyp2d41-ps	T	A	15: 82,782,035 (GRCm38)		noncoding transcript	Het
Dhx9	C	T	1: 153,472,707 (GRCm38)	V291M	probably benign	Het
Dmxl1	T	A	18: 49,878,082 (GRCm38)	V1102E	probably benign	Het
Dnah7a	A	T	1: 53,456,874 (GRCm38)		probably null	Het
Dock4	G	A	12: 40,737,360 (GRCm38)		probably benign	Het
Dpp3	G	T	19: 4,914,643 (GRCm38)	N545K	probably damaging	Het
Elmo2	T	A	2: 165,298,726 (GRCm38)	N275I	probably damaging	Het
Farp1	A	G	14: 121,255,513 (GRCm38)	K509R	probably benign	Het
Fat3	T	C	9: 15,965,979 (GRCm38)	Y3446C	possibly damaging	Het
Fgd2	T	A	17: 29,374,990 (GRCm38)		probably benign	Het
Flg	T	A	3: 93,277,721 (GRCm38)		probably benign	Het
Gas2l2	A	T	11: 83,421,910 (GRCm38)	W859R	probably damaging	Het
Gatm	T	C	2: 122,600,744 (GRCm38)	D254G	probably damaging	Het
Gjd4	T	A	18: 9,280,998 (GRCm38)	I27F	probably damaging	Het
Gsdmc2	C	A	15: 63,827,755 (GRCm38)		probably benign	Het
Haus5	C	T	7: 30,654,180 (GRCm38)	V591I	probably benign	Het
Kcnab3	G	A	11: 69,330,227 (GRCm38)		probably null	Het
Khdrbs2	A	G	1: 32,619,202 (GRCm38)	D281G	possibly damaging	Het
Krt86	A	T	15: 101,477,402 (GRCm38)	M393L	probably benign	Het
Limk1	T	C	5: 134,672,761 (GRCm38)	Y96C	probably damaging	Het
Lrp2bp	T	A	8: 46,013,155 (GRCm38)	Y100*	probably null	Het
Mamstr	T	G	7: 45,641,770 (GRCm38)		probably benign	Het
Man1a	A	G	10: 53,919,187 (GRCm38)	Y657H	probably damaging	Het
Marf1	G	A	16: 14,111,727 (GRCm38)	P1672S	possibly damaging	Het
Mboat7	T	C	7: 3,683,818 (GRCm38)	Y341C	probably damaging	Het
Nhsl1	A	T	10: 18,525,669 (GRCm38)	N881I	probably damaging	Het
Nox3	T	C	17: 3,682,961 (GRCm38)	Y225C	probably benign	Het
Nrp1	C	T	8: 128,500,608 (GRCm38)		probably benign	Het
Olfr1080	A	G	2: 86,553,632 (GRCm38)	F164S	probably damaging	Het
Olfr1214	C	T	2: 88,987,349 (GRCm38)	M284I	probably benign	Het
Olfr1260	C	T	2: 89,978,507 (GRCm38)	T243I	probably damaging	Het
Olfr1458	G	A	19: 13,103,278 (GRCm38)	R3C	possibly damaging	Het
Pcdhb13	A	T	18: 37,444,257 (GRCm38)	M563L	probably benign	Het
Pclo	C	T	5: 14,540,479 (GRCm38)	T931M	unknown	Het
Peli2	C	T	14: 48,121,202 (GRCm38)	P16S	possibly damaging	Het
Pfas	G	T	11: 68,990,467 (GRCm38)	R1025S	probably benign	Het
Pik3c2a	A	T	7: 116,354,072 (GRCm38)	I1196N	probably damaging	Het
Pmfbp1	A	T	8: 109,535,985 (GRCm38)		probably benign	Het
Prg4	T	C	1: 150,456,086 (GRCm38)	T279A	possibly damaging	Het
Psma1	A	T	7: 114,267,205 (GRCm38)		probably benign	Het
Rab11fip1	A	G	8: 27,153,121 (GRCm38)	L550P	probably damaging	Het
Rgs12	T	A	5: 34,965,320 (GRCm38)	I149N	probably damaging	Het
Rmnd5a	T	C	6: 71,399,231 (GRCm38)	H195R	probably damaging	Het
Rnf17	T	C	14: 56,471,373 (GRCm38)	L750P	probably damaging	Het
Rtcb	T	C	10: 85,957,656 (GRCm38)	N18D	probably benign	Het
Seh1l	T	C	18: 67,792,016 (GRCm38)		probably null	Het
Sis	T	G	3: 72,932,094 (GRCm38)	N813T	probably benign	Het
Sptbn2	T	C	19: 4,745,377 (GRCm38)		probably benign	Het
Stag3	C	T	5: 138,283,023 (GRCm38)		probably benign	Het
Taar2	G	A	10: 23,941,495 (GRCm38)	R311H	probably benign	Het
Taok3	C	T	5: 117,272,229 (GRCm38)	Q829*	probably null	Het
Tnfrsf21	C	T	17: 43,038,213 (GRCm38)	H239Y	probably benign	Het
Ttn	A	G	2: 76,951,542 (GRCm38)		probably benign	Het
Unc79	A	G	12: 103,125,681 (GRCm38)	E1756G	probably damaging	Het
Usp35	A	T	7: 97,313,597 (GRCm38)		probably null	Het
Vmn2r111	A	G	17: 22,548,009 (GRCm38)	F836L	probably benign	Het
Vmn2r77	A	G	7: 86,801,938 (GRCm38)	D344G	possibly damaging	Het
Zbtb40	A	G	4: 136,987,278 (GRCm38)	C1067R	probably damaging	Het

Other mutations in Olfr742

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02385:Olfr742	APN	14	50,515,443 (GRCm38)	missense	probably damaging	0.97
R0369:Olfr742	UTSW	14	50,515,825 (GRCm38)	missense	probably benign	0.00
R0612:Olfr742	UTSW	14	50,515,482 (GRCm38)	missense	probably benign
R1588:Olfr742	UTSW	14	50,516,127 (GRCm38)	missense	probably benign
R1726:Olfr742	UTSW	14	50,516,179 (GRCm38)	splice site	probably null
R1998:Olfr742	UTSW	14	50,515,356 (GRCm38)	missense	probably benign	0.00
R2875:Olfr742	UTSW	14	50,515,812 (GRCm38)	missense	probably benign	0.06
R4009:Olfr742	UTSW	14	50,515,962 (GRCm38)	missense	possibly damaging	0.77
R5119:Olfr742	UTSW	14	50,515,509 (GRCm38)	missense	probably benign	0.00
R5232:Olfr742	UTSW	14	50,516,038 (GRCm38)	missense	probably damaging	0.99
R5627:Olfr742	UTSW	14	50,515,800 (GRCm38)	missense	probably benign
R5930:Olfr742	UTSW	14	50,515,792 (GRCm38)	missense	probably benign
R6597:Olfr742	UTSW	14	50,515,551 (GRCm38)	missense	probably benign	0.11
R7441:Olfr742	UTSW	14	50,515,396 (GRCm38)	missense	probably damaging	1.00
R7596:Olfr742	UTSW	14	50,515,545 (GRCm38)	missense	probably benign	0.14
R8051:Olfr742	UTSW	14	50,515,643 (GRCm38)	missense	probably benign	0.00
R8294:Olfr742	UTSW	14	50,515,626 (GRCm38)	missense	possibly damaging	0.81
R8329:Olfr742	UTSW	14	50,515,558 (GRCm38)	missense	probably damaging	0.99
R8380:Olfr742	UTSW	14	50,515,840 (GRCm38)	missense	probably benign	0.00
R9517:Olfr742	UTSW	14	50,515,313 (GRCm38)	missense	probably benign
R9647:Olfr742	UTSW	14	50,516,095 (GRCm38)	missense	probably damaging	1.00
R9694:Olfr742	UTSW	14	50,515,212 (GRCm38)	missense	probably benign
R9796:Olfr742	UTSW	14	50,515,772 (GRCm38)	missense	probably damaging	1.00
Z1088:Olfr742	UTSW	14	50,515,527 (GRCm38)	missense	possibly damaging	0.90
Z1176:Olfr742	UTSW	14	50,516,065 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGGGCACCTCTGCAACATC -3'
(R):5'- aatgccacaacacaattcttttCGTTGA -3'

Sequencing Primer
(F):5'- GGTCCAGAATTATAGACCACTTCCTG -3'
(R):5'- GTGTATGTACTAGGGTAGTA -3'

Posted On

2013-05-29