Incidental Mutation 'R5592:Rpa3'
ID437568
Institutional Source Beutler Lab
Gene Symbol Rpa3
Ensembl Gene ENSMUSG00000012483
Gene Namereplication protein A3
Synonyms14kDa, C330026P08Rik
MMRRC Submission 043144-MU
Accession Numbers
Is this an essential gene? Probably essential (E-score: 0.753) question?
Stock #R5592 (G1)
Quality Score225
Status Validated
Chromosome6
Chromosomal Location8255936-8259173 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 8257694 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Lysine at position 56 (M56K)
Ref Sequence ENSEMBL: ENSMUSP00000012627 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000012627] [ENSMUST00000159335] [ENSMUST00000159378] [ENSMUST00000159433] [ENSMUST00000160705] [ENSMUST00000162034] [ENSMUST00000162564]
Predicted Effect probably benign
Transcript: ENSMUST00000012627
AA Change: M56K

PolyPhen 2 Score 0.300 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000012627
Gene: ENSMUSG00000012483
AA Change: M56K

DomainStartEndE-ValueType
Pfam:Rep_fac-A_3 5 113 2.5e-36 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000159335
Predicted Effect probably benign
Transcript: ENSMUST00000159378
Predicted Effect probably benign
Transcript: ENSMUST00000159433
Predicted Effect noncoding transcript
Transcript: ENSMUST00000159594
Predicted Effect probably benign
Transcript: ENSMUST00000160705
Predicted Effect noncoding transcript
Transcript: ENSMUST00000161400
Predicted Effect probably benign
Transcript: ENSMUST00000162034
SMART Domains Protein: ENSMUSP00000124306
Gene: ENSMUSG00000089862

DomainStartEndE-ValueType
low complexity region 40 51 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000162564
Meta Mutation Damage Score 0.0927 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.8%
  • 20x: 96.7%
Validation Efficiency 100% (48/48)
Allele List at MGI
Other mutations in this stock
Total: 48 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A630001G21Rik A T 1: 85,726,611 F2I probably damaging Het
Adam17 C T 12: 21,334,137 S454N probably damaging Het
Adcy1 A T 11: 7,139,088 K501* probably null Het
Arhgap28 A T 17: 67,858,272 M543K probably damaging Het
Art3 T A 5: 92,392,820 Y141N probably damaging Het
Ccdc38 C T 10: 93,550,202 T60I possibly damaging Het
Crmp1 A C 5: 37,265,265 I139L probably benign Het
Cul9 G A 17: 46,520,591 L1566F probably benign Het
Cysltr2 T C 14: 73,029,491 K260E probably benign Het
Dpcr1 T C 17: 35,643,643 Y9C probably damaging Het
Drd1 A T 13: 54,054,171 M1K probably null Het
Flii T G 11: 60,720,399 M449L probably benign Het
Galnt16 T C 12: 80,588,519 V343A probably damaging Het
Gimap4 A T 6: 48,691,158 E154D probably damaging Het
Glmp T G 3: 88,326,026 probably benign Het
Glt1d1 A T 5: 127,657,119 D119V probably benign Het
Gm5930 C T 14: 44,331,429 M245I probably benign Het
Gm7102 C T 19: 61,175,926 G24R unknown Het
Golgb1 T A 16: 36,925,763 H2901Q probably benign Het
Krt12 C T 11: 99,420,824 V184I probably benign Het
Lyst T G 13: 13,743,333 I3326S probably damaging Het
Mier3 T A 13: 111,706,661 Y182* probably null Het
Mlip G A 9: 77,230,482 S381L probably damaging Het
Mmp25 A G 17: 23,640,202 V157A possibly damaging Het
Ms4a6c A G 19: 11,480,277 probably benign Het
Ms4a6c A G 19: 11,481,132 probably benign Het
Muc19 T C 15: 91,930,314 noncoding transcript Het
Mycbp2 C A 14: 103,194,677 M2308I probably benign Het
Myo1a G T 10: 127,714,039 V463F probably damaging Het
Ogdh A T 11: 6,316,763 probably null Het
Olfr1157 A G 2: 87,962,340 L184P probably damaging Het
Olfr651 A T 7: 104,553,731 N271Y probably benign Het
Otx1 C A 11: 21,997,037 A91S probably damaging Het
Paip1 C A 13: 119,450,798 D124E probably damaging Het
Pdcd11 T A 19: 47,102,725 N379K probably benign Het
Pitpnm2 T C 5: 124,142,149 E112G probably damaging Het
Prrg4 T C 2: 104,832,778 Y161C probably benign Het
Rb1 A G 14: 73,211,747 Y648H probably damaging Het
Scarf1 A T 11: 75,525,687 T652S probably benign Het
Slc16a5 A G 11: 115,472,782 K423R probably benign Het
Sptan1 C T 2: 29,986,719 probably benign Het
Ssrp1 T C 2: 85,045,519 I574T probably benign Het
Synm A G 7: 67,759,516 L38P probably damaging Het
Ubqln4 T G 3: 88,556,864 M224R probably damaging Het
Uroc1 A G 6: 90,355,344 N561S probably damaging Het
Vav1 A G 17: 57,304,835 Y483C probably benign Het
Vps13a G A 19: 16,725,571 L673F probably damaging Het
Zfp418 G T 7: 7,181,315 K92N possibly damaging Het
Other mutations in Rpa3
AlleleSourceChrCoordTypePredicted EffectPPH Score
R1419:Rpa3 UTSW 6 8257720 missense probably benign 0.05
R6297:Rpa3 UTSW 6 8256767 nonsense probably null
Predicted Primers PCR Primer
(F):5'- ATGTTGGAGCCCTAAGAATCAC -3'
(R):5'- GCCTCGTTTAGTTTTAGTGTACCAAG -3'

Sequencing Primer
(F):5'- TGTTGGAGCCCTAAGAATCACTAAAG -3'
(R):5'- CTGGGCTTACAATCATCTGATCAGG -3'
Posted On2016-10-26