Mutagenetix > Incidental Mutation

Incidental Mutation 'R5611:Or10al5'

438054

Institutional Source

Beutler Lab

Gene Symbol

Or10al5

Ensembl Gene

ENSMUSG00000060017

Gene Name

olfactory receptor family 10 subfamily AL member 5

Synonyms

Olfr121, GA_x6K02T2PSCP-2211113-2212078, MOR263-4

MMRRC Submission

043273-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R5611 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

38059757-38063895 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 38062975 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 77 (I77F)

Ref Sequence

ENSEMBL: ENSMUSP00000084377 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074555] [ENSMUST00000174675]

AlphaFold

Q5CZY0

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074555
AA Change: I77F

PolyPhen 2 Score 0.942 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000084377
Gene: ENSMUSG00000060017
AA Change: I77F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	314	2.9e-56	PFAM
Pfam:7TM_GPCR_Srsx	41	311	2.1e-5	PFAM
Pfam:7tm_1	47	296	8.3e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000174675
AA Change: I77F

PolyPhen 2 Score 0.242 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000133865
Gene: ENSMUSG00000060017
AA Change: I77F

Domain	Start	End	E-Value	Type
transmembrane domain	33	55	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 96.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9930111J21Rik2	G	C	11: 48,910,828 (GRCm39)	T535R	possibly damaging	Het
Adam34	A	T	8: 44,104,749 (GRCm39)	F299I	probably benign	Het
Adamts20	A	G	15: 94,171,161 (GRCm39)	M1854T	possibly damaging	Het
Adrm1b	G	A	3: 92,335,758 (GRCm39)	P315S	probably damaging	Het
Apbb1	A	G	7: 105,208,690 (GRCm39)	V581A	probably damaging	Het
Apol6	T	A	15: 76,935,240 (GRCm39)		probably null	Het
Arhgef37	T	C	18: 61,640,334 (GRCm39)	T242A	probably benign	Het
Asxl2	T	C	12: 3,534,598 (GRCm39)	V265A	probably damaging	Het
Bicd1	A	T	6: 149,414,954 (GRCm39)	R556*	probably null	Het
C2	A	G	17: 35,091,360 (GRCm39)	I101T	probably damaging	Het
Cd22	T	A	7: 30,577,575 (GRCm39)		probably benign	Het
Cd33	T	C	7: 43,181,542 (GRCm39)	H206R	probably damaging	Het
Cd5l	G	A	3: 87,275,082 (GRCm39)	G207D	possibly damaging	Het
Chrd	A	T	16: 20,557,724 (GRCm39)	D774V	probably damaging	Het
Cmtm1	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	CGGCACGTACTGAAGGTCGCTGACTGGATGGTGTGGCACGTACTGAAGGTCGCTGACTGGATGGT	8: 105,036,102 (GRCm39)		probably benign	Het
Csn1s1	A	T	5: 87,825,503 (GRCm39)		probably null	Het
Dpyd	G	A	3: 118,987,942 (GRCm39)	V704I	probably benign	Het
Dscc1	C	A	15: 54,945,569 (GRCm39)	Q312H	probably benign	Het
Dysf	A	T	6: 84,041,860 (GRCm39)	T154S	probably damaging	Het
Foxi2	T	C	7: 135,013,433 (GRCm39)	V221A	probably benign	Het
Gabbr2	A	T	4: 46,804,105 (GRCm39)	I250N	probably damaging	Het
Gfap	T	A	11: 102,787,895 (GRCm39)	T17S	probably benign	Het
Hcrtr2	A	G	9: 76,230,596 (GRCm39)	V64A	probably damaging	Het
Igkv4-86	A	G	6: 68,887,659 (GRCm39)	S27P	probably damaging	Het
Kalrn	G	T	16: 33,996,150 (GRCm39)	F903L	probably damaging	Het
Lrrc31	A	G	3: 30,745,304 (GRCm39)		probably null	Het
Mlh3	T	C	12: 85,314,219 (GRCm39)	T656A	probably benign	Het
Mss51	G	T	14: 20,533,174 (GRCm39)	S432R	possibly damaging	Het
Mzf1	A	G	7: 12,778,554 (GRCm39)		probably benign	Het
Nop58	T	A	1: 59,749,672 (GRCm39)		probably benign	Het
Otogl	T	C	10: 107,622,630 (GRCm39)	E1652G	probably damaging	Het
Pikfyve	C	A	1: 65,295,247 (GRCm39)	N1459K	probably damaging	Het
Pkn3	G	A	2: 29,969,673 (GRCm39)	G61D	probably damaging	Het
Plekha4	T	C	7: 45,203,065 (GRCm39)	S581P	probably benign	Het
Ppm1g	A	G	5: 31,363,441 (GRCm39)	F256L	probably damaging	Het
Proser1	A	G	3: 53,386,296 (GRCm39)	N726S	probably benign	Het
Rapgef1	A	G	2: 29,592,448 (GRCm39)	D480G	probably damaging	Het
Reln	G	A	5: 22,244,663 (GRCm39)	Q772*	probably null	Het
Sh3gl2	T	C	4: 85,273,568 (GRCm39)	V40A	probably benign	Het
Slc22a23	G	A	13: 34,489,222 (GRCm39)	T221I	probably benign	Het
Slc22a28	T	A	19: 8,040,698 (GRCm39)	T518S	probably damaging	Het
Slc4a1ap	A	G	5: 31,711,173 (GRCm39)		probably benign	Het
St8sia4	T	C	1: 95,555,409 (GRCm39)	D207G	probably damaging	Het
Syde1	T	A	10: 78,421,725 (GRCm39)	T609S	probably benign	Het
Tbc1d5	A	G	17: 51,042,995 (GRCm39)	I831T	probably damaging	Het
Tle4	T	C	19: 14,427,159 (GRCm39)	D754G	probably damaging	Het
Ttn	C	T	2: 76,562,869 (GRCm39)	W26949*	probably null	Het
Vmn1r222	A	C	13: 23,416,743 (GRCm39)	S157A	probably damaging	Het
Vmn1r51	T	A	6: 90,106,692 (GRCm39)	L203M	probably benign	Het
Vmn1r6	T	C	6: 56,979,362 (GRCm39)	L8P	probably damaging	Het
Vmn2r103	A	G	17: 20,013,904 (GRCm39)	D232G	probably damaging	Het
Vmn2r17	T	A	5: 109,576,030 (GRCm39)	D300E	probably damaging	Het
Vmn2r66	T	A	7: 84,654,951 (GRCm39)	K453*	probably null	Het
Vps13a	A	T	19: 16,702,936 (GRCm39)	D672E	probably damaging	Het
Vps54	A	G	11: 21,261,130 (GRCm39)	N599S	possibly damaging	Het
Zc3h13	A	T	14: 75,568,348 (GRCm39)	N1214Y	probably benign	Het
Zc3h18	T	G	8: 123,135,109 (GRCm39)		probably null	Het
Zfp51	A	G	17: 21,684,354 (GRCm39)	E323G	probably damaging	Het

Other mutations in Or10al5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01348:Or10al5	APN	17	38,063,177 (GRCm39)	missense	probably benign	0.03
R0100:Or10al5	UTSW	17	38,063,594 (GRCm39)	missense	probably benign	0.12
R0328:Or10al5	UTSW	17	38,063,284 (GRCm39)	missense	possibly damaging	0.57
R1641:Or10al5	UTSW	17	38,062,916 (GRCm39)	missense	possibly damaging	0.90
R2358:Or10al5	UTSW	17	38,063,271 (GRCm39)	nonsense	probably null
R3711:Or10al5	UTSW	17	38,063,271 (GRCm39)	nonsense	probably null
R3712:Or10al5	UTSW	17	38,063,271 (GRCm39)	nonsense	probably null
R4658:Or10al5	UTSW	17	38,063,054 (GRCm39)	missense	probably damaging	1.00
R4913:Or10al5	UTSW	17	38,063,315 (GRCm39)	missense	possibly damaging	0.86
R5229:Or10al5	UTSW	17	38,063,192 (GRCm39)	missense	probably benign	0.00
R6159:Or10al5	UTSW	17	38,063,038 (GRCm39)	missense	probably damaging	1.00
R6375:Or10al5	UTSW	17	38,062,990 (GRCm39)	missense	probably benign
R6500:Or10al5	UTSW	17	38,063,577 (GRCm39)	missense	probably damaging	1.00
R6818:Or10al5	UTSW	17	38,063,315 (GRCm39)	missense	possibly damaging	0.86
R7027:Or10al5	UTSW	17	38,063,300 (GRCm39)	missense	probably damaging	1.00
R7498:Or10al5	UTSW	17	38,063,242 (GRCm39)	missense	probably damaging	0.96
R7888:Or10al5	UTSW	17	38,062,888 (GRCm39)	missense	probably damaging	1.00
R8299:Or10al5	UTSW	17	38,062,891 (GRCm39)	nonsense	probably null
R9497:Or10al5	UTSW	17	38,062,942 (GRCm39)	missense	probably damaging	1.00
Z1176:Or10al5	UTSW	17	38,063,658 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCTTGTCTGTCAAACGCTTTG -3'
(R):5'- TACCCCATGACTCATTCGTG -3'

Sequencing Primer
(F):5'- GTCAAACGCTTTGCATTTTCCAAG -3'
(R):5'- CATGACTCATTCGTGTTGCATAG -3'

Posted On

2016-10-26