Mutagenetix > Incidental Mutation

Incidental Mutation 'R5579:Zzef1'

438303

Institutional Source

Beutler Lab

Gene Symbol

Zzef1

Ensembl Gene

ENSMUSG00000055670

Gene Name

zinc finger, ZZ-type with EF hand domain 1

Synonyms

C130099L13Rik, 8430405D05Rik

MMRRC Submission

043267-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5579 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

72796226-72927120 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 72900637 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 2189 (V2189A)

Ref Sequence

ENSEMBL: ENSMUSP00000147028 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069395] [ENSMUST00000172220] [ENSMUST00000207107]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000069395
AA Change: V2160A

PolyPhen 2 Score 0.928 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000068790
Gene: ENSMUSG00000055670
AA Change: V2160A

Domain	Start	End	E-Value	Type
low complexity region	4	20	N/A	INTRINSIC
low complexity region	28	68	N/A	INTRINSIC
low complexity region	78	93	N/A	INTRINSIC
APC10	246	397	2.65e-48	SMART
internal_repeat_1	1122	1192	1.25e-7	PROSPERO
low complexity region	1472	1485	N/A	INTRINSIC
low complexity region	1512	1525	N/A	INTRINSIC
ZnF_ZZ	1775	1823	2.54e-7	SMART
ZnF_ZZ	1824	1868	1.2e-8	SMART
low complexity region	1947	1963	N/A	INTRINSIC
low complexity region	2127	2140	N/A	INTRINSIC
low complexity region	2249	2263	N/A	INTRINSIC
internal_repeat_1	2657	2726	1.25e-7	PROSPERO
low complexity region	2840	2853	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000172220
AA Change: V2160A

PolyPhen 2 Score 0.928 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000130515
Gene: ENSMUSG00000055670
AA Change: V2160A

Domain	Start	End	E-Value	Type
low complexity region	4	20	N/A	INTRINSIC
low complexity region	28	68	N/A	INTRINSIC
low complexity region	78	93	N/A	INTRINSIC
APC10	246	397	2.65e-48	SMART
internal_repeat_1	1006	1192	1.57e-16	PROSPERO
low complexity region	1472	1485	N/A	INTRINSIC
low complexity region	1512	1525	N/A	INTRINSIC
ZnF_ZZ	1775	1823	2.54e-7	SMART
ZnF_ZZ	1824	1868	1.2e-8	SMART
low complexity region	1947	1963	N/A	INTRINSIC
low complexity region	2127	2140	N/A	INTRINSIC
low complexity region	2249	2263	N/A	INTRINSIC
internal_repeat_1	2583	2759	1.57e-16	PROSPERO
low complexity region	2873	2886	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000207107
AA Change: V2189A

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 98.9%
20x: 97.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 114 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810403A07Rik	T	C	3: 88,700,275 (GRCm38)	S345P	probably benign	Het
6430573F11Rik	T	C	8: 36,512,041 (GRCm38)	V266A	probably benign	Het
Abca3	G	T	17: 24,376,729 (GRCm38)	C352F	probably damaging	Het
Abce1	A	T	8: 79,700,586 (GRCm38)	I237N	possibly damaging	Het
Acrbp	A	G	6: 125,061,099 (GRCm38)	D421G	probably benign	Het
Adam8	T	A	7: 139,988,984 (GRCm38)	Y201F	probably benign	Het
Adgrf1	T	A	17: 43,311,064 (GRCm38)	C731S	probably damaging	Het
AF067063	T	A	13: 119,828,415 (GRCm38)	M82L	probably benign	Het
Akap8l	A	T	17: 32,321,942 (GRCm38)	I529N	probably damaging	Het
Akap9	G	A	5: 4,064,714 (GRCm38)	G114D	possibly damaging	Het
Alox12b	T	A	11: 69,162,932 (GRCm38)	D158E	probably benign	Het
Ankrd11	A	G	8: 122,884,231 (GRCm38)	S2639P	probably damaging	Het
Ankrd42	T	C	7: 92,590,182 (GRCm38)	Y466C	possibly damaging	Het
Apobr	T	C	7: 126,587,675 (GRCm38)	I786T	probably benign	Het
Arhgef12	C	T	9: 43,010,193 (GRCm38)	G329R	probably benign	Het
Btnl1	T	C	17: 34,381,552 (GRCm38)		probably null	Het
Ccdc174	A	G	6: 91,881,350 (GRCm38)		probably null	Het
Ccdc183	T	C	2: 25,615,422 (GRCm38)	D177G	possibly damaging	Het
Cd14	A	G	18: 36,726,235 (GRCm38)	Y56H	probably benign	Het
Cep162	G	A	9: 87,203,671 (GRCm38)	A1200V	probably benign	Het
Clic3	T	C	2: 25,458,307 (GRCm38)	L128P	probably damaging	Het
Cntnap5b	A	T	1: 100,383,395 (GRCm38)	R538*	probably null	Het
Cntnap5b	A	T	1: 100,383,399 (GRCm38)	Q539L	probably benign	Het
Coq7	A	T	7: 118,517,335 (GRCm38)	N214K	unknown	Het
Cramp1l	G	A	17: 24,973,113 (GRCm38)	H1018Y	possibly damaging	Het
Crtac1	T	A	19: 42,304,806 (GRCm38)	D288V	probably damaging	Het
Dab2ip	C	T	2: 35,715,327 (GRCm38)	R132*	probably null	Het
Dis3l	C	A	9: 64,330,835 (GRCm38)	C125F	probably benign	Het
Dnmt1	A	T	9: 20,920,205 (GRCm38)	V543D	probably damaging	Het
Dock9	A	T	14: 121,599,695 (GRCm38)	L67Q	probably damaging	Het
Ehbp1	A	T	11: 22,137,846 (GRCm38)	S413T	probably damaging	Het
Endov	A	G	11: 119,505,097 (GRCm38)	I158V	probably benign	Het
Epb41l4b	A	G	4: 57,064,802 (GRCm38)	V469A	possibly damaging	Het
Etf1	G	A	18: 34,913,601 (GRCm38)	P119S	probably damaging	Het
Fam135a	A	G	1: 24,029,727 (GRCm38)	L491P	possibly damaging	Het
Fancd2	A	T	6: 113,560,051 (GRCm38)		probably null	Het
Fank1	T	A	7: 133,869,329 (GRCm38)		probably null	Het
Fbxo18	A	G	2: 11,748,993 (GRCm38)	I846T	probably damaging	Het
Gas2l3	T	C	10: 89,414,066 (GRCm38)	T397A	probably benign	Het
Gga1	T	C	15: 78,893,188 (GRCm38)	V513A	probably damaging	Het
Ggt1	T	C	10: 75,585,948 (GRCm38)	V543A	probably damaging	Het
Gm11596	A	T	11: 99,792,891 (GRCm38)	C134*	probably null	Het
Gnal	C	T	18: 67,088,771 (GRCm38)	R82C	unknown	Het
Hat1	T	C	2: 71,410,238 (GRCm38)	V92A	possibly damaging	Het
Icosl	C	T	10: 78,073,763 (GRCm38)	R181C	probably damaging	Het
Ift46	A	G	9: 44,786,863 (GRCm38)	M208V	possibly damaging	Het
Ighv1-26	T	C	12: 114,788,599 (GRCm38)	K42E	possibly damaging	Het
Ipo5	A	G	14: 120,938,613 (GRCm38)	K617E	probably benign	Het
Irx5	A	G	8: 92,359,913 (GRCm38)	D208G	probably benign	Het
Itgal	T	G	7: 127,306,929 (GRCm38)	V397G	probably benign	Het
Itpr2	G	A	6: 146,173,366 (GRCm38)	R2297*	probably null	Het
Itpr3	A	G	17: 27,113,519 (GRCm38)	D1779G	probably damaging	Het
Krt4	T	A	15: 101,921,234 (GRCm38)	E286D	probably benign	Het
Loxl4	C	T	19: 42,604,290 (GRCm38)	G317E	probably damaging	Het
Mapk6	T	G	9: 75,388,062 (GRCm38)	H718P	possibly damaging	Het
Mark2	A	C	19: 7,282,816 (GRCm38)	V14G	probably damaging	Het
Mbd5	A	G	2: 49,272,814 (GRCm38)	T1103A	possibly damaging	Het
Mcph1	A	G	8: 18,632,293 (GRCm38)	E482G	probably benign	Het
Mme	G	A	3: 63,348,645 (GRCm38)	E509K	probably damaging	Het
Mrgbp	A	G	2: 180,585,483 (GRCm38)	T204A	probably damaging	Het
Mycbp2	A	T	14: 103,291,333 (GRCm38)	F430I	probably damaging	Het
Myo6	A	G	9: 80,217,720 (GRCm38)	D27G	probably damaging	Het
Ncoa6	A	G	2: 155,406,677 (GRCm38)	L1569S	probably damaging	Het
Ndc1	T	A	4: 107,380,704 (GRCm38)	F235Y	possibly damaging	Het
Nmur2	A	T	11: 56,033,009 (GRCm38)	S240T	probably benign	Het
Olfr1176	A	T	2: 88,340,413 (GRCm38)	I283F	possibly damaging	Het
Olfr301	T	A	7: 86,412,726 (GRCm38)	Y121*	probably null	Het
Olfr608	G	A	7: 103,470,914 (GRCm38)	V292M	probably damaging	Het
Osbpl1a	G	T	18: 12,841,192 (GRCm38)	A62E	probably damaging	Het
Osbpl1a	A	C	18: 12,892,262 (GRCm38)	S333A	probably benign	Het
Otud4	C	T	8: 79,664,108 (GRCm38)	T417I	probably benign	Het
Pagr1a	T	C	7: 127,015,442 (GRCm38)	E197G	probably damaging	Het
Pcdh18	T	C	3: 49,744,977 (GRCm38)	Q1012R	probably damaging	Het
Pcdhgb5	A	C	18: 37,731,637 (GRCm38)	I162L	probably benign	Het
Pdpr	T	A	8: 111,123,816 (GRCm38)	Y462N	probably damaging	Het
Pkhd1	A	T	1: 20,523,142 (GRCm38)	H1582Q	probably damaging	Het
Polr1b	A	G	2: 129,110,108 (GRCm38)	K352R	probably damaging	Het
Poteg	G	A	8: 27,448,037 (GRCm38)	V12M	probably damaging	Het
Pou2f1	A	C	1: 165,915,162 (GRCm38)	V54G	probably damaging	Het
Ppme1	A	G	7: 100,344,975 (GRCm38)	L177P	probably damaging	Het
Prkce	A	G	17: 86,619,948 (GRCm38)	D550G	probably damaging	Het
Prrc2c	A	G	1: 162,680,758 (GRCm38)		probably null	Het
Ptpn22	A	G	3: 103,882,139 (GRCm38)		probably null	Het
Rabif	G	A	1: 134,506,205 (GRCm38)	V86M	probably damaging	Het
Rbm25	T	A	12: 83,668,507 (GRCm38)	M484K	probably benign	Het
Rcn2	G	A	9: 56,057,429 (GRCm38)	A224T	probably benign	Het
Rexo5	T	A	7: 119,834,403 (GRCm38)		probably null	Het
Rnase1	A	T	14: 51,145,762 (GRCm38)	I45N	probably benign	Het
Rnmt	T	A	18: 68,306,115 (GRCm38)	D98E	possibly damaging	Het
Rprd2	A	G	3: 95,785,059 (GRCm38)	F188L	probably damaging	Het
Scube2	G	A	7: 109,810,737 (GRCm38)	T643M	probably damaging	Het
Slc7a15	T	C	12: 8,539,344 (GRCm38)	I68V	probably benign	Het
Slco1a5	A	T	6: 142,242,125 (GRCm38)	V496D	possibly damaging	Het
Slitrk6	T	G	14: 110,751,217 (GRCm38)	S353R	possibly damaging	Het
Smpd5	T	A	15: 76,295,125 (GRCm38)	I53K	possibly damaging	Het
Tas2r108	T	C	6: 40,494,087 (GRCm38)	S166P	probably benign	Het
Tecpr1	C	A	5: 144,214,344 (GRCm38)	V245L	possibly damaging	Het
Tenm3	G	T	8: 48,236,764 (GRCm38)	N1929K	probably damaging	Het
Tigd5	T	G	15: 75,911,025 (GRCm38)	F412C	probably damaging	Het
Timm17a	A	T	1: 135,306,188 (GRCm38)	S74T	possibly damaging	Het
Tle1	A	T	4: 72,139,808 (GRCm38)	L60Q	probably damaging	Het
Tmem132d	T	C	5: 127,796,000 (GRCm38)	E515G	possibly damaging	Het
Tmem65	T	C	15: 58,794,397 (GRCm38)	N115S	probably benign	Het
Trmt5	C	A	12: 73,281,652 (GRCm38)	R259L	possibly damaging	Het
Ttc37	T	C	13: 76,185,200 (GRCm38)	W25R	probably damaging	Het
Vmn2r50	A	T	7: 10,050,089 (GRCm38)	W153R	probably benign	Het
Vwa3a	T	G	7: 120,768,173 (GRCm38)	S184A	probably benign	Het
Zbtb25	A	G	12: 76,349,164 (GRCm38)	L428P	possibly damaging	Het
Zbtb49	C	T	5: 38,200,816 (GRCm38)	D698N	probably damaging	Het
Zbtb8os	T	G	4: 129,340,735 (GRCm38)	D35E	probably damaging	Het
Zfp316	T	C	5: 143,264,491 (GRCm38)	T56A	unknown	Het
Zfp334	A	T	2: 165,380,487 (GRCm38)	C545*	probably null	Het
Zmiz1	C	A	14: 25,644,856 (GRCm38)	S247R	probably damaging	Het
Zscan18	A	G	7: 12,775,381 (GRCm38)		probably benign	Het

Other mutations in Zzef1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Zzef1	APN	11	72,875,126 (GRCm38)	missense	probably benign	0.02
IGL00898:Zzef1	APN	11	72,875,173 (GRCm38)	missense	probably benign	0.00
IGL00970:Zzef1	APN	11	72,915,245 (GRCm38)	missense	probably benign	0.06
IGL01062:Zzef1	APN	11	72,874,969 (GRCm38)	missense	probably benign
IGL01832:Zzef1	APN	11	72,875,066 (GRCm38)	missense	probably damaging	0.99
IGL02005:Zzef1	APN	11	72,888,299 (GRCm38)	missense	probably benign	0.00
IGL02026:Zzef1	APN	11	72,881,338 (GRCm38)	missense	probably benign	0.39
IGL02110:Zzef1	APN	11	72,913,112 (GRCm38)	missense	probably damaging	1.00
IGL02305:Zzef1	APN	11	72,866,597 (GRCm38)	splice site	probably benign
IGL02308:Zzef1	APN	11	72,886,747 (GRCm38)	missense	probably benign	0.04
IGL02315:Zzef1	APN	11	72,875,257 (GRCm38)	nonsense	probably null
IGL02332:Zzef1	APN	11	72,916,509 (GRCm38)	missense	probably benign	0.01
IGL02389:Zzef1	APN	11	72,891,217 (GRCm38)	missense	probably benign
IGL02389:Zzef1	APN	11	72,899,538 (GRCm38)	missense	possibly damaging	0.89
IGL02451:Zzef1	APN	11	72,901,388 (GRCm38)	missense	probably damaging	0.99
IGL02541:Zzef1	APN	11	72,872,649 (GRCm38)	missense	probably damaging	1.00
IGL02950:Zzef1	APN	11	72,917,699 (GRCm38)	splice site	probably benign
IGL02953:Zzef1	APN	11	72,855,398 (GRCm38)	missense	probably benign
IGL03053:Zzef1	APN	11	72,831,539 (GRCm38)	splice site	probably benign
IGL03085:Zzef1	APN	11	72,855,524 (GRCm38)	splice site	probably benign
IGL03152:Zzef1	APN	11	72,923,182 (GRCm38)	critical splice donor site	probably null
IGL03329:Zzef1	APN	11	72,917,273 (GRCm38)	splice site	probably benign
IGL03376:Zzef1	APN	11	72,876,551 (GRCm38)	splice site	probably benign
IGL03394:Zzef1	APN	11	72,886,775 (GRCm38)	splice site	probably null
Dreidel	UTSW	11	72,908,469 (GRCm38)	nonsense	probably null
Hanukkah	UTSW	11	72,893,332 (GRCm38)	missense	probably benign	0.00
Mezuzah	UTSW	11	72,848,733 (GRCm38)	nonsense	probably null
BB005:Zzef1	UTSW	11	72,821,896 (GRCm38)	missense	probably damaging	0.99
BB015:Zzef1	UTSW	11	72,821,896 (GRCm38)	missense	probably damaging	0.99
PIT4508001:Zzef1	UTSW	11	72,895,176 (GRCm38)	missense	probably benign
PIT4581001:Zzef1	UTSW	11	72,899,672 (GRCm38)	missense	probably benign	0.00
PIT4810001:Zzef1	UTSW	11	72,850,745 (GRCm38)	missense	probably damaging	1.00
R0094:Zzef1	UTSW	11	72,817,965 (GRCm38)	missense	probably benign	0.01
R0119:Zzef1	UTSW	11	72,821,851 (GRCm38)	missense	probably benign
R0136:Zzef1	UTSW	11	72,821,851 (GRCm38)	missense	probably benign
R0140:Zzef1	UTSW	11	72,899,551 (GRCm38)	missense	possibly damaging	0.70
R0212:Zzef1	UTSW	11	72,873,910 (GRCm38)	missense	possibly damaging	0.66
R0217:Zzef1	UTSW	11	72,889,068 (GRCm38)	missense	probably damaging	1.00
R0220:Zzef1	UTSW	11	72,865,966 (GRCm38)	missense	probably damaging	1.00
R0304:Zzef1	UTSW	11	72,880,624 (GRCm38)	missense	probably benign	0.10
R0400:Zzef1	UTSW	11	72,895,242 (GRCm38)	missense	probably damaging	1.00
R0422:Zzef1	UTSW	11	72,866,091 (GRCm38)	missense	possibly damaging	0.93
R0471:Zzef1	UTSW	11	72,923,111 (GRCm38)	missense	probably damaging	1.00
R0557:Zzef1	UTSW	11	72,917,730 (GRCm38)	missense	probably damaging	1.00
R0581:Zzef1	UTSW	11	72,851,900 (GRCm38)	missense	probably benign	0.00
R0599:Zzef1	UTSW	11	72,913,178 (GRCm38)	missense	probably damaging	1.00
R0603:Zzef1	UTSW	11	72,818,069 (GRCm38)	missense	probably benign	0.00
R0657:Zzef1	UTSW	11	72,821,851 (GRCm38)	missense	probably benign
R0987:Zzef1	UTSW	11	72,901,333 (GRCm38)	small deletion	probably benign
R1246:Zzef1	UTSW	11	72,874,909 (GRCm38)	missense	probably benign	0.00
R1327:Zzef1	UTSW	11	72,893,414 (GRCm38)	critical splice donor site	probably null
R1438:Zzef1	UTSW	11	72,912,945 (GRCm38)	missense	probably damaging	0.96
R1466:Zzef1	UTSW	11	72,924,679 (GRCm38)	missense	probably damaging	1.00
R1466:Zzef1	UTSW	11	72,924,679 (GRCm38)	missense	probably damaging	1.00
R1485:Zzef1	UTSW	11	72,900,809 (GRCm38)	splice site	probably null
R1556:Zzef1	UTSW	11	72,915,233 (GRCm38)	missense	probably damaging	1.00
R1563:Zzef1	UTSW	11	72,848,733 (GRCm38)	nonsense	probably null
R1584:Zzef1	UTSW	11	72,924,679 (GRCm38)	missense	probably damaging	1.00
R1643:Zzef1	UTSW	11	72,826,202 (GRCm38)	missense	probably damaging	1.00
R1646:Zzef1	UTSW	11	72,864,036 (GRCm38)	critical splice donor site	probably null
R1764:Zzef1	UTSW	11	72,893,332 (GRCm38)	missense	probably benign	0.00
R1777:Zzef1	UTSW	11	72,910,272 (GRCm38)	missense	probably damaging	1.00
R1793:Zzef1	UTSW	11	72,886,709 (GRCm38)	missense	probably damaging	1.00
R1900:Zzef1	UTSW	11	72,848,714 (GRCm38)	missense	probably damaging	0.99
R2096:Zzef1	UTSW	11	72,872,639 (GRCm38)	missense	probably benign	0.02
R2134:Zzef1	UTSW	11	72,880,624 (GRCm38)	missense	probably benign	0.02
R2157:Zzef1	UTSW	11	72,848,634 (GRCm38)	splice site	probably benign
R2183:Zzef1	UTSW	11	72,886,718 (GRCm38)	nonsense	probably null
R2192:Zzef1	UTSW	11	72,910,156 (GRCm38)	splice site	probably null
R2230:Zzef1	UTSW	11	72,884,416 (GRCm38)	missense	probably damaging	0.99
R2259:Zzef1	UTSW	11	72,900,633 (GRCm38)	nonsense	probably null
R2384:Zzef1	UTSW	11	72,858,394 (GRCm38)	missense	probably damaging	0.99
R2426:Zzef1	UTSW	11	72,915,265 (GRCm38)	missense	probably benign	0.01
R2915:Zzef1	UTSW	11	72,910,326 (GRCm38)	splice site	probably null
R3700:Zzef1	UTSW	11	72,886,772 (GRCm38)	missense	probably null	1.00
R3875:Zzef1	UTSW	11	72,889,040 (GRCm38)	missense	probably benign	0.22
R3902:Zzef1	UTSW	11	72,908,500 (GRCm38)	missense	probably damaging	1.00
R3927:Zzef1	UTSW	11	72,858,382 (GRCm38)	missense	probably damaging	1.00
R4086:Zzef1	UTSW	11	72,875,053 (GRCm38)	missense	probably benign	0.02
R4301:Zzef1	UTSW	11	72,889,035 (GRCm38)	missense	probably damaging	0.96
R4359:Zzef1	UTSW	11	72,823,508 (GRCm38)	missense	probably damaging	0.98
R4382:Zzef1	UTSW	11	72,875,112 (GRCm38)	missense	probably benign	0.00
R4453:Zzef1	UTSW	11	72,872,639 (GRCm38)	missense	probably benign	0.02
R4466:Zzef1	UTSW	11	72,924,659 (GRCm38)	missense	probably damaging	1.00
R4471:Zzef1	UTSW	11	72,913,331 (GRCm38)	missense	probably damaging	1.00
R4510:Zzef1	UTSW	11	72,888,170 (GRCm38)	missense	probably benign	0.32
R4511:Zzef1	UTSW	11	72,888,170 (GRCm38)	missense	probably benign	0.32
R4714:Zzef1	UTSW	11	72,837,212 (GRCm38)	missense	probably damaging	1.00
R4799:Zzef1	UTSW	11	72,859,623 (GRCm38)	missense	probably benign	0.12
R4906:Zzef1	UTSW	11	72,901,388 (GRCm38)	missense	probably damaging	1.00
R5075:Zzef1	UTSW	11	72,858,344 (GRCm38)	missense	probably damaging	1.00
R5357:Zzef1	UTSW	11	72,843,333 (GRCm38)	nonsense	probably null
R5598:Zzef1	UTSW	11	72,916,521 (GRCm38)	missense	probably damaging	1.00
R5725:Zzef1	UTSW	11	72,855,482 (GRCm38)	missense	possibly damaging	0.86
R5765:Zzef1	UTSW	11	72,821,937 (GRCm38)	nonsense	probably null
R5928:Zzef1	UTSW	11	72,912,852 (GRCm38)	missense	probably damaging	1.00
R6003:Zzef1	UTSW	11	72,824,065 (GRCm38)	splice site	probably null
R6047:Zzef1	UTSW	11	72,866,095 (GRCm38)	missense	probably damaging	0.99
R6224:Zzef1	UTSW	11	72,855,383 (GRCm38)	missense	probably damaging	0.99
R6225:Zzef1	UTSW	11	72,869,805 (GRCm38)	missense	possibly damaging	0.62
R6287:Zzef1	UTSW	11	72,923,112 (GRCm38)	missense	probably damaging	1.00
R6361:Zzef1	UTSW	11	72,884,349 (GRCm38)	missense	possibly damaging	0.93
R6451:Zzef1	UTSW	11	72,923,156 (GRCm38)	missense	possibly damaging	0.88
R6467:Zzef1	UTSW	11	72,911,264 (GRCm38)	critical splice donor site	probably null
R6484:Zzef1	UTSW	11	72,895,271 (GRCm38)	missense	probably damaging	1.00
R6493:Zzef1	UTSW	11	72,913,303 (GRCm38)	missense	probably benign	0.06
R6520:Zzef1	UTSW	11	72,826,065 (GRCm38)	missense	probably damaging	1.00
R6527:Zzef1	UTSW	11	72,874,990 (GRCm38)	missense	probably benign	0.00
R6540:Zzef1	UTSW	11	72,913,229 (GRCm38)	missense	probably damaging	1.00
R6608:Zzef1	UTSW	11	72,912,826 (GRCm38)	missense	probably damaging	1.00
R6795:Zzef1	UTSW	11	72,850,659 (GRCm38)	missense	probably benign	0.00
R6927:Zzef1	UTSW	11	72,913,157 (GRCm38)	missense	probably damaging	1.00
R6987:Zzef1	UTSW	11	72,855,514 (GRCm38)	missense	possibly damaging	0.89
R7048:Zzef1	UTSW	11	72,866,699 (GRCm38)	nonsense	probably null
R7076:Zzef1	UTSW	11	72,899,559 (GRCm38)	missense	probably benign	0.00
R7099:Zzef1	UTSW	11	72,872,649 (GRCm38)	missense	possibly damaging	0.92
R7132:Zzef1	UTSW	11	72,917,871 (GRCm38)	critical splice donor site	probably null
R7175:Zzef1	UTSW	11	72,851,901 (GRCm38)	missense	possibly damaging	0.49
R7284:Zzef1	UTSW	11	72,886,690 (GRCm38)	missense	probably damaging	0.99
R7300:Zzef1	UTSW	11	72,875,004 (GRCm38)	missense	probably benign	0.02
R7486:Zzef1	UTSW	11	72,864,786 (GRCm38)	missense	possibly damaging	0.85
R7503:Zzef1	UTSW	11	72,826,067 (GRCm38)	missense	probably damaging	1.00
R7679:Zzef1	UTSW	11	72,893,278 (GRCm38)	missense	probably benign
R7874:Zzef1	UTSW	11	72,859,653 (GRCm38)	missense	probably benign	0.01
R7898:Zzef1	UTSW	11	72,796,547 (GRCm38)	missense	probably damaging	1.00
R7928:Zzef1	UTSW	11	72,821,896 (GRCm38)	missense	probably damaging	0.99
R8021:Zzef1	UTSW	11	72,823,416 (GRCm38)	missense	probably damaging	0.99
R8145:Zzef1	UTSW	11	72,908,469 (GRCm38)	nonsense	probably null
R8255:Zzef1	UTSW	11	72,875,129 (GRCm38)	missense	probably benign	0.00
R8303:Zzef1	UTSW	11	72,917,189 (GRCm38)	missense	probably damaging	1.00
R8492:Zzef1	UTSW	11	72,886,746 (GRCm38)	missense	probably damaging	0.97
R8492:Zzef1	UTSW	11	72,872,604 (GRCm38)	missense	probably damaging	1.00
R8498:Zzef1	UTSW	11	72,853,322 (GRCm38)	missense	probably damaging	1.00
R8547:Zzef1	UTSW	11	72,844,441 (GRCm38)	missense	probably damaging	1.00
R8874:Zzef1	UTSW	11	72,863,989 (GRCm38)	missense	probably benign	0.00
R8885:Zzef1	UTSW	11	72,796,576 (GRCm38)	missense	probably benign	0.00
R8972:Zzef1	UTSW	11	72,900,673 (GRCm38)	missense	probably damaging	1.00
R8979:Zzef1	UTSW	11	72,875,177 (GRCm38)	missense	probably benign	0.00
R9053:Zzef1	UTSW	11	72,922,476 (GRCm38)	missense	probably benign
R9108:Zzef1	UTSW	11	72,899,778 (GRCm38)	missense	probably benign	0.11
R9121:Zzef1	UTSW	11	72,866,120 (GRCm38)	nonsense	probably null
R9253:Zzef1	UTSW	11	72,848,637 (GRCm38)	splice site	probably benign
R9370:Zzef1	UTSW	11	72,853,322 (GRCm38)	missense	probably damaging	1.00
R9408:Zzef1	UTSW	11	72,864,827 (GRCm38)	missense	possibly damaging	0.86
R9467:Zzef1	UTSW	11	72,916,425 (GRCm38)	missense	probably damaging	1.00
R9468:Zzef1	UTSW	11	72,923,183 (GRCm38)	critical splice donor site	probably null
R9563:Zzef1	UTSW	11	72,874,906 (GRCm38)	missense	probably damaging	1.00
R9647:Zzef1	UTSW	11	72,869,825 (GRCm38)	missense	probably benign	0.01
R9667:Zzef1	UTSW	11	72,867,960 (GRCm38)	missense	probably benign
R9742:Zzef1	UTSW	11	72,858,353 (GRCm38)	missense	probably benign
X0028:Zzef1	UTSW	11	72,906,979 (GRCm38)	missense	probably benign	0.29
Z1176:Zzef1	UTSW	11	72,796,528 (GRCm38)	missense	probably damaging	1.00
Z1177:Zzef1	UTSW	11	72,900,631 (GRCm38)	critical splice acceptor site	probably null
Z1177:Zzef1	UTSW	11	72,826,178 (GRCm38)	missense	probably damaging	1.00
Z1177:Zzef1	UTSW	11	72,796,312 (GRCm38)	missense	possibly damaging	0.91
Z1177:Zzef1	UTSW	11	72,915,320 (GRCm38)	missense	probably damaging	1.00
Z1186:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1187:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1188:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1189:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1190:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1191:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign
Z1192:Zzef1	UTSW	11	72,889,182 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACATGTGAGGATGACACTCTAGAG -3'
(R):5'- TTCAGATGCTGTACCTGGGG -3'

Sequencing Primer
(F):5'- ACACTTACCCATGGTGTGATGAC -3'
(R):5'- ATGCTGTACCTGGGGGAAGC -3'

Posted On

2016-10-26