Mutagenetix > Incidental Mutation

Incidental Mutation 'R5585:Zfp142'

438641

Institutional Source

Beutler Lab

Gene Symbol

Zfp142

Ensembl Gene

ENSMUSG00000026135

Gene Name

zinc finger protein 142

Synonyms

9330177B18Rik

MMRRC Submission

043139-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5585 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

74565127-74588246 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 74578245 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 150 (Q150*)

Ref Sequence

ENSEMBL: ENSMUSP00000138085 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027315] [ENSMUST00000066986] [ENSMUST00000113737] [ENSMUST00000127921] [ENSMUST00000156613]

AlphaFold

G5E869

Predicted Effect

probably benign
Transcript: ENSMUST00000027315
AA Change: A309V

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000027315
Gene: ENSMUSG00000026135
AA Change: A309V

Domain	Start	End	E-Value	Type
ZnF_C2H2	103	127	1.16e1	SMART
ZnF_C2H2	164	186	1.26e-2	SMART
ZnF_C2H2	193	218	7.78e-3	SMART
ZnF_C2H2	223	247	2.29e0	SMART
ZnF_C2H2	250	272	9.96e-1	SMART
low complexity region	317	329	N/A	INTRINSIC
ZnF_C2H2	363	385	9.96e-1	SMART
ZnF_C2H2	391	411	1.26e1	SMART
ZnF_C2H2	419	442	1.47e-3	SMART
ZnF_C2H2	453	475	2.75e-3	SMART
ZnF_C2H2	486	511	3.34e-2	SMART
ZnF_C2H2	516	540	4.81e0	SMART
ZnF_C2H2	543	566	7.05e-1	SMART
ZnF_C2H2	572	595	2.17e-1	SMART
ZnF_C2H2	601	623	1.56e-2	SMART
ZnF_C2H2	629	651	8.94e-3	SMART
ZnF_C2H2	657	679	1.38e-3	SMART
ZnF_C2H2	685	707	9.44e-2	SMART
ZnF_C2H2	712	735	1.26e-2	SMART
ZnF_C2H2	744	767	1.31e0	SMART
ZnF_C2H2	773	796	2.63e0	SMART
low complexity region	903	919	N/A	INTRINSIC
low complexity region	953	969	N/A	INTRINSIC
low complexity region	996	1007	N/A	INTRINSIC
ZnF_C2H2	1039	1059	2.01e1	SMART
ZnF_C2H2	1069	1089	1.91e1	SMART
low complexity region	1147	1160	N/A	INTRINSIC
ZnF_C2H2	1187	1207	2.7e2	SMART
low complexity region	1209	1222	N/A	INTRINSIC
ZnF_C2H2	1265	1285	3.56e1	SMART
ZnF_C2H2	1295	1318	4.98e-1	SMART
ZnF_C2H2	1331	1354	2.49e-1	SMART
ZnF_C2H2	1360	1382	1.03e-2	SMART
ZnF_C2H2	1388	1411	5.72e-1	SMART
ZnF_C2H2	1417	1440	6.75e0	SMART
ZnF_C2H2	1446	1469	9.58e-3	SMART
ZnF_C2H2	1488	1511	1.64e-1	SMART
ZnF_C2H2	1514	1536	1.1e-2	SMART
ZnF_C2H2	1540	1563	4.05e-1	SMART
ZnF_C2H2	1580	1602	4.45e0	SMART
ZnF_C2H2	1608	1630	8.81e-2	SMART
ZnF_C2H2	1636	1658	1.18e-2	SMART
ZnF_C2H2	1664	1686	1.2e-3	SMART
ZnF_C2H2	1692	1715	3.89e-3	SMART
ZnF_C2H2	1721	1743	4.54e-4	SMART
ZnF_C2H2	1749	1771	1.18e-2	SMART
ZnF_C2H2	1777	1799	3.52e-1	SMART
low complexity region	1829	1838	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000066986
AA Change: A108V

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000065149
Gene: ENSMUSG00000026135
AA Change: A108V

Domain	Start	End	E-Value	Type
low complexity region	116	128	N/A	INTRINSIC
ZnF_C2H2	162	184	9.96e-1	SMART
ZnF_C2H2	190	210	1.26e1	SMART
ZnF_C2H2	218	241	1.47e-3	SMART
ZnF_C2H2	252	274	2.75e-3	SMART
ZnF_C2H2	285	310	3.34e-2	SMART
ZnF_C2H2	315	339	4.81e0	SMART
ZnF_C2H2	342	365	7.05e-1	SMART
ZnF_C2H2	371	394	2.17e-1	SMART
ZnF_C2H2	400	422	1.56e-2	SMART
ZnF_C2H2	428	450	8.94e-3	SMART
ZnF_C2H2	456	478	1.38e-3	SMART
ZnF_C2H2	484	506	9.44e-2	SMART
ZnF_C2H2	511	534	1.26e-2	SMART
ZnF_C2H2	543	566	1.31e0	SMART
ZnF_C2H2	572	595	2.63e0	SMART
low complexity region	702	718	N/A	INTRINSIC
low complexity region	752	768	N/A	INTRINSIC
low complexity region	795	806	N/A	INTRINSIC
ZnF_C2H2	838	858	2.01e1	SMART
ZnF_C2H2	868	888	1.91e1	SMART
low complexity region	946	959	N/A	INTRINSIC
ZnF_C2H2	986	1006	2.7e2	SMART
low complexity region	1008	1021	N/A	INTRINSIC
ZnF_C2H2	1064	1084	3.56e1	SMART
ZnF_C2H2	1094	1117	4.98e-1	SMART
ZnF_C2H2	1130	1153	2.49e-1	SMART
ZnF_C2H2	1159	1181	1.03e-2	SMART
ZnF_C2H2	1187	1210	5.72e-1	SMART
ZnF_C2H2	1216	1239	6.75e0	SMART
ZnF_C2H2	1245	1268	9.58e-3	SMART
ZnF_C2H2	1287	1310	1.64e-1	SMART
ZnF_C2H2	1313	1335	1.1e-2	SMART
ZnF_C2H2	1339	1362	4.05e-1	SMART
ZnF_C2H2	1379	1401	4.45e0	SMART
ZnF_C2H2	1407	1429	8.81e-2	SMART
ZnF_C2H2	1435	1457	1.18e-2	SMART
ZnF_C2H2	1463	1485	1.2e-3	SMART
ZnF_C2H2	1491	1514	3.89e-3	SMART
ZnF_C2H2	1520	1542	4.54e-4	SMART
ZnF_C2H2	1548	1570	1.18e-2	SMART
ZnF_C2H2	1576	1598	3.52e-1	SMART
low complexity region	1628	1637	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113737
AA Change: A206V

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000109366
Gene: ENSMUSG00000026135
AA Change: A206V

Domain	Start	End	E-Value	Type
ZnF_C2H2	93	115	9.81e1	SMART
ZnF_C2H2	120	144	2.29e0	SMART
ZnF_C2H2	147	169	9.96e-1	SMART
low complexity region	214	226	N/A	INTRINSIC
ZnF_C2H2	260	282	9.96e-1	SMART
ZnF_C2H2	288	308	1.26e1	SMART
ZnF_C2H2	316	339	1.47e-3	SMART
ZnF_C2H2	350	372	2.75e-3	SMART
ZnF_C2H2	383	408	3.34e-2	SMART
ZnF_C2H2	413	437	4.81e0	SMART
ZnF_C2H2	440	463	7.05e-1	SMART
ZnF_C2H2	469	492	2.17e-1	SMART
ZnF_C2H2	498	520	1.56e-2	SMART
ZnF_C2H2	526	548	8.94e-3	SMART
ZnF_C2H2	554	576	1.38e-3	SMART
ZnF_C2H2	582	604	9.44e-2	SMART
ZnF_C2H2	609	632	1.26e-2	SMART
ZnF_C2H2	641	664	1.31e0	SMART
ZnF_C2H2	670	693	2.63e0	SMART
low complexity region	800	816	N/A	INTRINSIC
low complexity region	850	866	N/A	INTRINSIC
low complexity region	893	904	N/A	INTRINSIC
ZnF_C2H2	936	956	2.01e1	SMART
ZnF_C2H2	966	986	1.91e1	SMART
low complexity region	1044	1057	N/A	INTRINSIC
ZnF_C2H2	1084	1104	2.7e2	SMART
low complexity region	1106	1119	N/A	INTRINSIC
ZnF_C2H2	1162	1182	3.56e1	SMART
ZnF_C2H2	1192	1215	4.98e-1	SMART
ZnF_C2H2	1228	1251	2.49e-1	SMART
ZnF_C2H2	1257	1279	1.03e-2	SMART
ZnF_C2H2	1285	1308	5.72e-1	SMART
ZnF_C2H2	1314	1337	6.75e0	SMART
ZnF_C2H2	1343	1366	9.58e-3	SMART
ZnF_C2H2	1385	1408	1.64e-1	SMART
ZnF_C2H2	1411	1433	1.1e-2	SMART
ZnF_C2H2	1437	1460	4.05e-1	SMART
ZnF_C2H2	1477	1499	4.45e0	SMART
ZnF_C2H2	1505	1527	8.81e-2	SMART
ZnF_C2H2	1533	1555	1.18e-2	SMART
ZnF_C2H2	1561	1583	1.2e-3	SMART
ZnF_C2H2	1589	1612	3.89e-3	SMART
ZnF_C2H2	1618	1640	4.54e-4	SMART
ZnF_C2H2	1646	1668	1.18e-2	SMART
ZnF_C2H2	1674	1696	3.52e-1	SMART
low complexity region	1726	1735	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000127921

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130112

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136865

Predicted Effect

probably null
Transcript: ENSMUST00000156613
AA Change: Q150*

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the Kruppel family of C2H2-type zinc finger proteins. It contains 31 C2H2-type zinc fingers and may be involved in transcriptional regulation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600014C23Rik	A	T	17: 45,733,744	I17N	unknown	Het
4932414N04Rik	A	G	2: 68,741,426	T549A	probably benign	Het
9330159F19Rik	C	A	10: 29,225,275	S548Y	possibly damaging	Het
Aanat	A	G	11: 116,596,973	Y166C	probably damaging	Het
Adra2a	G	T	19: 54,046,239	A9S	probably benign	Het
Ap4m1	A	G	5: 138,172,240	Y17C	probably damaging	Het
Arhgap33	T	G	7: 30,523,835	M891L	probably benign	Het
Calm5	A	G	13: 3,854,372	D22G	possibly damaging	Het
Ccdc15	C	T	9: 37,277,403	R795H	probably benign	Het
Cngb1	T	A	8: 95,263,139	I323F	probably damaging	Het
Cyp26b1	A	G	6: 84,577,189	F74L	probably damaging	Het
Dpagt1	G	A	9: 44,329,142		probably null	Het
Ercc8	C	T	13: 108,175,589	P196S	probably damaging	Het
Gm10985	A	C	3: 53,845,253	Y19S	probably damaging	Het
Hfm1	G	A	5: 106,911,439	S239L	probably benign	Het
Hgf	A	G	5: 16,564,801	D91G	possibly damaging	Het
Lefty2	T	A	1: 180,893,263	V27D	possibly damaging	Het
Lrp2	G	A	2: 69,464,624	T3450I	possibly damaging	Het
Lrrc38	A	G	4: 143,350,391	I75V	probably damaging	Het
Ncor2	C	A	5: 125,067,911	E556*	probably null	Het
Nedd9	A	G	13: 41,316,474	L401P	probably damaging	Het
Nfatc4	T	C	14: 55,826,755	L163P	probably damaging	Het
Nln	T	C	13: 104,025,061	N667S	possibly damaging	Het
Olfr1153	A	G	2: 87,896,675	T159A	possibly damaging	Het
Pnpla8	T	C	12: 44,283,064	I133T	probably benign	Het
Psma1	C	T	7: 114,274,067	G12S	probably damaging	Het
Psmd3	G	A	11: 98,682,881	G51D	possibly damaging	Het
Ptprb	A	G	10: 116,380,854	Q1959R	probably damaging	Het
Rhbdf1	A	G	11: 32,210,222		probably null	Het
Rnf167	T	C	11: 70,649,482	V110A	probably damaging	Het
Rrp9	C	T	9: 106,485,326	S470F	probably benign	Het
Rtn3	G	A	19: 7,458,195	P125L	probably benign	Het
Scube1	C	T	15: 83,676,923	C156Y	probably damaging	Het
Tgm2	A	T	2: 158,131,455	Y245*	probably null	Het
Timeless	T	C	10: 128,240,243	I68T	probably damaging	Het
Ttn	A	G	2: 76,814,710	S12934P	probably damaging	Het
Vwa5b2	T	A	16: 20,594,678	Y214*	probably null	Het
Yars2	T	A	16: 16,304,620	N7K	probably damaging	Het
Zfp995	C	T	17: 21,887,358		probably benign	Het

Other mutations in Zfp142

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00581:Zfp142	APN	1	74566972	missense	probably damaging	1.00
IGL00711:Zfp142	APN	1	74572434	missense	probably damaging	1.00
IGL01391:Zfp142	APN	1	74579540	missense	probably damaging	1.00
IGL01808:Zfp142	APN	1	74576025	missense	probably damaging	1.00
IGL02074:Zfp142	APN	1	74569863	missense	probably damaging	0.97
IGL02134:Zfp142	APN	1	74569863	missense	probably damaging	0.97
IGL02567:Zfp142	APN	1	74578147	missense	possibly damaging	0.84
IGL02567:Zfp142	APN	1	74578149	missense	possibly damaging	0.92
IGL02567:Zfp142	APN	1	74578150	missense	possibly damaging	0.92
IGL02669:Zfp142	APN	1	74571273	missense	probably benign	0.18
IGL02694:Zfp142	APN	1	74570148	missense	probably damaging	0.98
IGL02884:Zfp142	APN	1	74571983	missense	probably damaging	0.99
IGL03000:Zfp142	APN	1	74573618	missense	probably benign	0.01
IGL03080:Zfp142	APN	1	74571209	missense	probably benign	0.18
IGL03238:Zfp142	APN	1	74576278	missense	probably benign	0.37
IGL03277:Zfp142	APN	1	74571034	missense	probably damaging	1.00
PIT4402001:Zfp142	UTSW	1	74579528	missense	probably damaging	1.00
R0124:Zfp142	UTSW	1	74568623	missense	probably damaging	1.00
R0256:Zfp142	UTSW	1	74578158	missense	probably benign
R0267:Zfp142	UTSW	1	74576064	missense	probably benign	0.05
R0306:Zfp142	UTSW	1	74570182	missense	probably damaging	1.00
R0321:Zfp142	UTSW	1	74569714	missense	probably damaging	1.00
R0466:Zfp142	UTSW	1	74585411	missense	possibly damaging	0.95
R0513:Zfp142	UTSW	1	74571555	missense	probably damaging	1.00
R1084:Zfp142	UTSW	1	74571826	missense	probably benign
R1534:Zfp142	UTSW	1	74572088	missense	probably benign	0.00
R1548:Zfp142	UTSW	1	74570104	missense	probably damaging	1.00
R1623:Zfp142	UTSW	1	74571775	missense	possibly damaging	0.56
R1628:Zfp142	UTSW	1	74571888	missense	possibly damaging	0.94
R1710:Zfp142	UTSW	1	74572230	missense	probably damaging	1.00
R1722:Zfp142	UTSW	1	74569776	missense	probably damaging	1.00
R1770:Zfp142	UTSW	1	74579631	missense	probably damaging	0.99
R2042:Zfp142	UTSW	1	74570619	missense	probably benign	0.10
R2112:Zfp142	UTSW	1	74573636	missense	probably damaging	1.00
R2249:Zfp142	UTSW	1	74567032	missense	probably damaging	1.00
R4393:Zfp142	UTSW	1	74572060	missense	probably benign	0.00
R4700:Zfp142	UTSW	1	74570272	missense	probably damaging	1.00
R4750:Zfp142	UTSW	1	74572458	missense	probably damaging	1.00
R4763:Zfp142	UTSW	1	74576512	missense	probably damaging	1.00
R5208:Zfp142	UTSW	1	74570868	missense	probably benign	0.18
R5233:Zfp142	UTSW	1	74585449	missense	probably damaging	1.00
R6192:Zfp142	UTSW	1	74570508	missense	probably damaging	1.00
R6461:Zfp142	UTSW	1	74567185	missense	probably damaging	1.00
R6482:Zfp142	UTSW	1	74570217	splice site	probably null
R7320:Zfp142	UTSW	1	74570008	nonsense	probably null
R7438:Zfp142	UTSW	1	74585520	missense	probably benign
R7528:Zfp142	UTSW	1	74570902	missense	probably benign	0.00
R7600:Zfp142	UTSW	1	74573668	missense	probably damaging	1.00
R8007:Zfp142	UTSW	1	74571496	missense	probably benign	0.01
R8681:Zfp142	UTSW	1	74571588	missense	probably damaging	1.00
R8772:Zfp142	UTSW	1	74571666	missense	possibly damaging	0.87
R9058:Zfp142	UTSW	1	74569796	nonsense	probably null
R9233:Zfp142	UTSW	1	74571129	missense	probably benign
R9281:Zfp142	UTSW	1	74568572	missense	probably damaging	1.00
R9302:Zfp142	UTSW	1	74567143	missense	probably damaging	0.98
R9454:Zfp142	UTSW	1	74569857	missense	probably damaging	1.00
R9569:Zfp142	UTSW	1	74576227	missense	probably damaging	1.00
R9595:Zfp142	UTSW	1	74572303	missense	probably damaging	1.00
R9680:Zfp142	UTSW	1	74571774	missense	probably benign	0.16
Z1177:Zfp142	UTSW	1	74570166	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGCCAGGATCCTCACTATCCAC -3'
(R):5'- TCTGACAGTCAAGAGTCTGAGG -3'

Sequencing Primer
(F):5'- ACTCCTCTCTCCTATGAAGAGC -3'
(R):5'- TCCCTGTCTTGAGTAAGAACAC -3'

Posted On

2016-10-26