Mutagenetix > Incidental Mutation

Incidental Mutation 'R5586:Gpr85'

438709

Institutional Source

Beutler Lab

Gene Symbol

Gpr85

Ensembl Gene

ENSMUSG00000048216

Gene Name

G protein-coupled receptor 85

Synonyms

MMRRC Submission

043140-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.260) question?

Stock #

R5586 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

13834458-13839942 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 13836001 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 301 (Y301*)

Ref Sequence

ENSEMBL: ENSMUSP00000111155 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060442] [ENSMUST00000115491] [ENSMUST00000115492]

AlphaFold

P60894

Predicted Effect

probably null
Transcript: ENSMUST00000060442
AA Change: Y301*

SMART Domains

Protein: ENSMUSP00000053837
Gene: ENSMUSG00000048216
AA Change: Y301*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	37	338	4.1e-38	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000115491
AA Change: Y301*

SMART Domains

Protein: ENSMUSP00000111154
Gene: ENSMUSG00000048216
AA Change: Y301*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	37	338	4.1e-38	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000115492
AA Change: Y301*

SMART Domains

Protein: ENSMUSP00000111155
Gene: ENSMUSG00000048216
AA Change: Y301*

Domain	Start	End	E-Value	Type
Pfam:7tm_1	37	338	1.6e-32	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127072

Meta Mutation Damage Score

0.9717

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.6%
20x: 96.0%

Validation Efficiency

98% (101/103)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Members of the G protein-coupled receptor (GPCR) family, such as GPR85, have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli, such as neurotransmitters, hormones, or light, induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins (Matsumoto et al., 2000 [PubMed 10833454]).[supplied by OMIM, Aug 2008]
PHENOTYPE: Mice homozygous for a knock-out allele display a significant increase in brain weight and enhanced contextual memory in a fear-conditioning task but no additional behavioral abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930432E11Rik	A	G	7: 29,577,728 (GRCm38)		noncoding transcript	Het
Aaas	A	T	15: 102,346,676 (GRCm38)		probably null	Het
Abcc3	G	A	11: 94,364,421 (GRCm38)	R600W	probably damaging	Het
Abhd13	A	T	8: 9,988,318 (GRCm38)	Q305L	probably benign	Het
Adcy5	A	T	16: 35,157,116 (GRCm38)	I340F	probably damaging	Het
Adgrl3	T	A	5: 81,724,147 (GRCm38)	I964N	probably damaging	Het
Anks1b	T	A	10: 90,077,064 (GRCm38)	H316Q	probably damaging	Het
Ap3d1	A	T	10: 80,719,130 (GRCm38)	F454I	possibly damaging	Het
Apol7c	T	C	15: 77,526,399 (GRCm38)	R116G	possibly damaging	Het
Arhgap5	A	G	12: 52,519,912 (GRCm38)	E1222G	possibly damaging	Het
AW551984	A	G	9: 39,591,263 (GRCm38)	V673A	probably benign	Het
Baiap2l1	A	G	5: 144,282,139 (GRCm38)	S220P	probably damaging	Het
Bcl6	A	G	16: 23,973,176 (GRCm38)	F143L	probably benign	Het
Calb1	A	T	4: 15,900,811 (GRCm38)	T165S	probably benign	Het
Ccdc66	C	A	14: 27,506,711 (GRCm38)	G6C	probably damaging	Het
Ccdc88a	A	G	11: 29,503,484 (GRCm38)	I344V	probably benign	Het
Cdh19	T	A	1: 110,929,857 (GRCm38)	D249V	probably damaging	Het
Ces1c	T	A	8: 93,127,599 (GRCm38)	T103S	probably benign	Het
Cfap54	T	A	10: 92,972,611 (GRCm38)	K1401*	probably null	Het
Copa	T	A	1: 172,105,222 (GRCm38)	N371K	probably damaging	Het
Cyp2b10	A	T	7: 25,917,012 (GRCm38)	Y348F	probably damaging	Het
Dennd5a	T	C	7: 109,905,721 (GRCm38)	R861G	possibly damaging	Het
Dhx8	T	C	11: 101,733,036 (GRCm38)		probably benign	Het
Dido1	C	T	2: 180,659,652 (GRCm38)	W2153*	probably null	Het
Dlgap1	T	A	17: 70,818,161 (GRCm38)	V969D	probably damaging	Het
Dvl3	A	G	16: 20,517,289 (GRCm38)	D32G	probably damaging	Het
Epdr1	T	C	13: 19,594,548 (GRCm38)	D24G	probably benign	Het
Etnk2	T	A	1: 133,379,305 (GRCm38)		probably null	Het
Fam129a	C	T	1: 151,717,556 (GRCm38)	T664I	probably benign	Het
Fhad1	C	T	4: 141,905,131 (GRCm38)	M1232I	probably benign	Het
Gcnt2	A	C	13: 40,860,953 (GRCm38)	E200A	probably damaging	Het
Gm12800	T	A	4: 101,910,120 (GRCm38)	F189I	probably benign	Het
Gm5174	A	G	10: 86,656,545 (GRCm38)		noncoding transcript	Het
Gm6408	T	A	5: 146,484,457 (GRCm38)	F299I	possibly damaging	Het
Gucy2e	G	T	11: 69,226,256 (GRCm38)	P780T	probably damaging	Het
Icam5	A	T	9: 21,034,820 (GRCm38)	N316I	probably damaging	Het
Ifit1bl1	C	T	19: 34,594,277 (GRCm38)	R260Q	probably damaging	Het
Il1r1	A	C	1: 40,225,251 (GRCm38)		probably benign	Het
Kif3c	A	T	12: 3,389,656 (GRCm38)	I86F	probably benign	Het
Klhdc2	A	G	12: 69,307,693 (GRCm38)		probably null	Het
Mast2	A	G	4: 116,435,563 (GRCm38)	L9P	probably damaging	Het
Mcoln1	G	T	8: 3,510,389 (GRCm38)	C316F	probably damaging	Het
Mon1a	A	T	9: 107,898,695 (GRCm38)	D4V	probably damaging	Het
Ms4a18	T	A	19: 11,013,674 (GRCm38)	M19L	probably benign	Het
Nbea	T	C	3: 55,631,971 (GRCm38)	K2790E	probably benign	Het
Noc3l	C	T	19: 38,814,695 (GRCm38)	E167K	possibly damaging	Het
Nol10	G	A	12: 17,416,828 (GRCm38)	E570K	possibly damaging	Het
Nr2e3	T	C	9: 59,949,201 (GRCm38)	R69G	probably damaging	Het
Obscn	G	A	11: 59,001,468 (GRCm38)	R1358*	probably null	Het
Olfr115	A	T	17: 37,610,254 (GRCm38)	F166I	probably damaging	Het
Olfr1472	A	T	19: 13,454,382 (GRCm38)	M45K	probably benign	Het
Olfr1535	C	A	13: 21,555,096 (GRCm38)	V309F	probably damaging	Het
Olfr675	A	G	7: 105,024,221 (GRCm38)	I253T	probably damaging	Het
Pak2	A	T	16: 32,041,519 (GRCm38)	D175E	probably benign	Het
Pccb	C	T	9: 100,985,803 (GRCm38)	V357I	possibly damaging	Het
Pcdhb4	C	T	18: 37,308,981 (GRCm38)	P448L	probably damaging	Het
Pcdhb9	A	G	18: 37,401,114 (GRCm38)	M54V	probably benign	Het
Ppp3cb	A	G	14: 20,520,690 (GRCm38)		probably benign	Het
Ppp4r1	A	G	17: 65,824,568 (GRCm38)	D452G	probably benign	Het
Prmt3	T	A	7: 49,826,751 (GRCm38)	D369E	probably damaging	Het
Psmd12	T	A	11: 107,486,475 (GRCm38)	V120D	probably benign	Het
Ptprb	T	C	10: 116,353,827 (GRCm38)	L1797P	probably damaging	Het
Ptprm	A	G	17: 66,920,196 (GRCm38)	S653P	probably damaging	Het
Pxdn	T	A	12: 30,003,142 (GRCm38)	V926D	probably damaging	Het
Retreg3	T	G	11: 101,106,339 (GRCm38)	Q105P	probably damaging	Het
Sacs	A	T	14: 61,206,441 (GRCm38)	R1979*	probably null	Het
Scn2a	T	A	2: 65,707,295 (GRCm38)	L696*	probably null	Het
Sema3c	A	T	5: 17,711,424 (GRCm38)	N465Y	probably damaging	Het
Slc25a32	A	T	15: 39,099,913 (GRCm38)	V171E	possibly damaging	Het
Slc30a7	C	T	3: 115,990,051 (GRCm38)	V158I	probably benign	Het
Slc44a5	G	A	3: 154,270,165 (GRCm38)		probably benign	Het
Slc4a8	A	G	15: 100,787,164 (GRCm38)	D140G	probably damaging	Het
Slc7a11	G	A	3: 50,443,083 (GRCm38)	S60L	possibly damaging	Het
Spryd3	A	T	15: 102,131,937 (GRCm38)	H59Q	probably benign	Het
Sqstm1	T	C	11: 50,203,022 (GRCm38)	D256G	probably damaging	Het
Sst	A	T	16: 23,889,737 (GRCm38)	S115T	probably damaging	Het
Surf1	T	C	2: 26,915,951 (GRCm38)		probably benign	Het
Synj1	A	T	16: 91,009,977 (GRCm38)		probably benign	Het
Tet1	C	A	10: 62,878,294 (GRCm38)	C574F	probably damaging	Het
Thnsl1	T	A	2: 21,212,390 (GRCm38)	Y318*	probably null	Het
Tmem110	A	G	14: 30,870,819 (GRCm38)	K166E	probably damaging	Het
Tomm70a	A	G	16: 57,122,130 (GRCm38)	E90G	probably damaging	Het
Treml4	A	G	17: 48,264,899 (GRCm38)	D110G	probably damaging	Het
Ttn	A	T	2: 76,811,243 (GRCm38)	L5176Q	possibly damaging	Het
Txnl1	C	T	18: 63,664,325 (GRCm38)	G283D	probably damaging	Het
Uba6	T	C	5: 86,135,047 (GRCm38)	D559G	probably damaging	Het
Usp4	T	C	9: 108,356,462 (GRCm38)	V94A	possibly damaging	Het
Vmn2r25	A	C	6: 123,825,296 (GRCm38)	C549W	probably damaging	Het
Vmn2r59	G	T	7: 42,045,681 (GRCm38)	Q436K	probably benign	Het
Wdr70	A	T	15: 7,884,288 (GRCm38)	Y627N	possibly damaging	Het
Xpr1	T	C	1: 155,312,863 (GRCm38)	I344V	probably benign	Het
Zfp423	T	A	8: 87,859,340 (GRCm38)	Q61L	possibly damaging	Het

Other mutations in Gpr85

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02414:Gpr85	APN	6	13,836,910 (GRCm38)	utr 5 prime	probably benign
R0784:Gpr85	UTSW	6	13,836,749 (GRCm38)	missense	probably benign	0.25
R1356:Gpr85	UTSW	6	13,836,147 (GRCm38)	missense	probably benign	0.42
R2343:Gpr85	UTSW	6	13,836,696 (GRCm38)	missense	probably damaging	1.00
R3934:Gpr85	UTSW	6	13,836,045 (GRCm38)	missense	probably benign	0.02
R3935:Gpr85	UTSW	6	13,836,045 (GRCm38)	missense	probably benign	0.02
R3936:Gpr85	UTSW	6	13,836,045 (GRCm38)	missense	probably benign	0.02
R4925:Gpr85	UTSW	6	13,835,978 (GRCm38)	missense	probably benign	0.26
R5313:Gpr85	UTSW	6	13,836,302 (GRCm38)	missense	probably damaging	1.00
R7043:Gpr85	UTSW	6	13,835,877 (GRCm38)	missense	probably damaging	1.00
R8458:Gpr85	UTSW	6	13,836,849 (GRCm38)	missense	probably benign
R8468:Gpr85	UTSW	6	13,836,296 (GRCm38)	missense	probably damaging	1.00
R8503:Gpr85	UTSW	6	13,836,830 (GRCm38)	missense	probably benign	0.01
R9519:Gpr85	UTSW	6	13,836,999 (GRCm38)	start gained	probably benign

Predicted Primers

PCR Primer
(F):5'- CTGGATTTTCTGCAGTAAAGAAGGG -3'
(R):5'- AACTGGACCTTTCATGGCCC -3'

Sequencing Primer
(F):5'- TCTGCAGTAAAGAAGGGTTGTGC -3'
(R):5'- CTTTCATGGCCCTGGAGCTAG -3'

Posted On

2016-10-26