Incidental Mutation 'R5586:Gpr85'
ID 438709
Institutional Source Beutler Lab
Gene Symbol Gpr85
Ensembl Gene ENSMUSG00000048216
Gene Name G protein-coupled receptor 85
MMRRC Submission 043140-MU
Accession Numbers
Essential gene? Possibly non essential (E-score: 0.260) question?
Stock # R5586 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 13834458-13839942 bp(-) (GRCm38)
Type of Mutation nonsense
DNA Base Change (assembly) G to T at 13836001 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Stop codon at position 301 (Y301*)
Ref Sequence ENSEMBL: ENSMUSP00000111155 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000060442] [ENSMUST00000115491] [ENSMUST00000115492]
AlphaFold P60894
Predicted Effect probably null
Transcript: ENSMUST00000060442
AA Change: Y301*
SMART Domains Protein: ENSMUSP00000053837
Gene: ENSMUSG00000048216
AA Change: Y301*

Pfam:7tm_1 37 338 4.1e-38 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000115491
AA Change: Y301*
SMART Domains Protein: ENSMUSP00000111154
Gene: ENSMUSG00000048216
AA Change: Y301*

Pfam:7tm_1 37 338 4.1e-38 PFAM
Predicted Effect probably null
Transcript: ENSMUST00000115492
AA Change: Y301*
SMART Domains Protein: ENSMUSP00000111155
Gene: ENSMUSG00000048216
AA Change: Y301*

Pfam:7tm_1 37 338 1.6e-32 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000127072
Meta Mutation Damage Score 0.9717 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.7%
  • 10x: 98.6%
  • 20x: 96.0%
Validation Efficiency 98% (101/103)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Members of the G protein-coupled receptor (GPCR) family, such as GPR85, have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli, such as neurotransmitters, hormones, or light, induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins (Matsumoto et al., 2000 [PubMed 10833454]).[supplied by OMIM, Aug 2008]
PHENOTYPE: Mice homozygous for a knock-out allele display a significant increase in brain weight and enhanced contextual memory in a fear-conditioning task but no additional behavioral abnormalities. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 92 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930432E11Rik A G 7: 29,577,728 (GRCm38) noncoding transcript Het
Aaas A T 15: 102,346,676 (GRCm38) probably null Het
Abcc3 G A 11: 94,364,421 (GRCm38) R600W probably damaging Het
Abhd13 A T 8: 9,988,318 (GRCm38) Q305L probably benign Het
Adcy5 A T 16: 35,157,116 (GRCm38) I340F probably damaging Het
Adgrl3 T A 5: 81,724,147 (GRCm38) I964N probably damaging Het
Anks1b T A 10: 90,077,064 (GRCm38) H316Q probably damaging Het
Ap3d1 A T 10: 80,719,130 (GRCm38) F454I possibly damaging Het
Apol7c T C 15: 77,526,399 (GRCm38) R116G possibly damaging Het
Arhgap5 A G 12: 52,519,912 (GRCm38) E1222G possibly damaging Het
AW551984 A G 9: 39,591,263 (GRCm38) V673A probably benign Het
Baiap2l1 A G 5: 144,282,139 (GRCm38) S220P probably damaging Het
Bcl6 A G 16: 23,973,176 (GRCm38) F143L probably benign Het
Calb1 A T 4: 15,900,811 (GRCm38) T165S probably benign Het
Ccdc66 C A 14: 27,506,711 (GRCm38) G6C probably damaging Het
Ccdc88a A G 11: 29,503,484 (GRCm38) I344V probably benign Het
Cdh19 T A 1: 110,929,857 (GRCm38) D249V probably damaging Het
Ces1c T A 8: 93,127,599 (GRCm38) T103S probably benign Het
Cfap54 T A 10: 92,972,611 (GRCm38) K1401* probably null Het
Copa T A 1: 172,105,222 (GRCm38) N371K probably damaging Het
Cyp2b10 A T 7: 25,917,012 (GRCm38) Y348F probably damaging Het
Dennd5a T C 7: 109,905,721 (GRCm38) R861G possibly damaging Het
Dhx8 T C 11: 101,733,036 (GRCm38) probably benign Het
Dido1 C T 2: 180,659,652 (GRCm38) W2153* probably null Het
Dlgap1 T A 17: 70,818,161 (GRCm38) V969D probably damaging Het
Dvl3 A G 16: 20,517,289 (GRCm38) D32G probably damaging Het
Epdr1 T C 13: 19,594,548 (GRCm38) D24G probably benign Het
Etnk2 T A 1: 133,379,305 (GRCm38) probably null Het
Fam129a C T 1: 151,717,556 (GRCm38) T664I probably benign Het
Fhad1 C T 4: 141,905,131 (GRCm38) M1232I probably benign Het
Gcnt2 A C 13: 40,860,953 (GRCm38) E200A probably damaging Het
Gm12800 T A 4: 101,910,120 (GRCm38) F189I probably benign Het
Gm5174 A G 10: 86,656,545 (GRCm38) noncoding transcript Het
Gm6408 T A 5: 146,484,457 (GRCm38) F299I possibly damaging Het
Gucy2e G T 11: 69,226,256 (GRCm38) P780T probably damaging Het
Icam5 A T 9: 21,034,820 (GRCm38) N316I probably damaging Het
Ifit1bl1 C T 19: 34,594,277 (GRCm38) R260Q probably damaging Het
Il1r1 A C 1: 40,225,251 (GRCm38) probably benign Het
Kif3c A T 12: 3,389,656 (GRCm38) I86F probably benign Het
Klhdc2 A G 12: 69,307,693 (GRCm38) probably null Het
Mast2 A G 4: 116,435,563 (GRCm38) L9P probably damaging Het
Mcoln1 G T 8: 3,510,389 (GRCm38) C316F probably damaging Het
Mon1a A T 9: 107,898,695 (GRCm38) D4V probably damaging Het
Ms4a18 T A 19: 11,013,674 (GRCm38) M19L probably benign Het
Nbea T C 3: 55,631,971 (GRCm38) K2790E probably benign Het
Noc3l C T 19: 38,814,695 (GRCm38) E167K possibly damaging Het
Nol10 G A 12: 17,416,828 (GRCm38) E570K possibly damaging Het
Nr2e3 T C 9: 59,949,201 (GRCm38) R69G probably damaging Het
Obscn G A 11: 59,001,468 (GRCm38) R1358* probably null Het
Olfr115 A T 17: 37,610,254 (GRCm38) F166I probably damaging Het
Olfr1472 A T 19: 13,454,382 (GRCm38) M45K probably benign Het
Olfr1535 C A 13: 21,555,096 (GRCm38) V309F probably damaging Het
Olfr675 A G 7: 105,024,221 (GRCm38) I253T probably damaging Het
Pak2 A T 16: 32,041,519 (GRCm38) D175E probably benign Het
Pccb C T 9: 100,985,803 (GRCm38) V357I possibly damaging Het
Pcdhb4 C T 18: 37,308,981 (GRCm38) P448L probably damaging Het
Pcdhb9 A G 18: 37,401,114 (GRCm38) M54V probably benign Het
Ppp3cb A G 14: 20,520,690 (GRCm38) probably benign Het
Ppp4r1 A G 17: 65,824,568 (GRCm38) D452G probably benign Het
Prmt3 T A 7: 49,826,751 (GRCm38) D369E probably damaging Het
Psmd12 T A 11: 107,486,475 (GRCm38) V120D probably benign Het
Ptprb T C 10: 116,353,827 (GRCm38) L1797P probably damaging Het
Ptprm A G 17: 66,920,196 (GRCm38) S653P probably damaging Het
Pxdn T A 12: 30,003,142 (GRCm38) V926D probably damaging Het
Retreg3 T G 11: 101,106,339 (GRCm38) Q105P probably damaging Het
Sacs A T 14: 61,206,441 (GRCm38) R1979* probably null Het
Scn2a T A 2: 65,707,295 (GRCm38) L696* probably null Het
Sema3c A T 5: 17,711,424 (GRCm38) N465Y probably damaging Het
Slc25a32 A T 15: 39,099,913 (GRCm38) V171E possibly damaging Het
Slc30a7 C T 3: 115,990,051 (GRCm38) V158I probably benign Het
Slc44a5 G A 3: 154,270,165 (GRCm38) probably benign Het
Slc4a8 A G 15: 100,787,164 (GRCm38) D140G probably damaging Het
Slc7a11 G A 3: 50,443,083 (GRCm38) S60L possibly damaging Het
Spryd3 A T 15: 102,131,937 (GRCm38) H59Q probably benign Het
Sqstm1 T C 11: 50,203,022 (GRCm38) D256G probably damaging Het
Sst A T 16: 23,889,737 (GRCm38) S115T probably damaging Het
Surf1 T C 2: 26,915,951 (GRCm38) probably benign Het
Synj1 A T 16: 91,009,977 (GRCm38) probably benign Het
Tet1 C A 10: 62,878,294 (GRCm38) C574F probably damaging Het
Thnsl1 T A 2: 21,212,390 (GRCm38) Y318* probably null Het
Tmem110 A G 14: 30,870,819 (GRCm38) K166E probably damaging Het
Tomm70a A G 16: 57,122,130 (GRCm38) E90G probably damaging Het
Treml4 A G 17: 48,264,899 (GRCm38) D110G probably damaging Het
Ttn A T 2: 76,811,243 (GRCm38) L5176Q possibly damaging Het
Txnl1 C T 18: 63,664,325 (GRCm38) G283D probably damaging Het
Uba6 T C 5: 86,135,047 (GRCm38) D559G probably damaging Het
Usp4 T C 9: 108,356,462 (GRCm38) V94A possibly damaging Het
Vmn2r25 A C 6: 123,825,296 (GRCm38) C549W probably damaging Het
Vmn2r59 G T 7: 42,045,681 (GRCm38) Q436K probably benign Het
Wdr70 A T 15: 7,884,288 (GRCm38) Y627N possibly damaging Het
Xpr1 T C 1: 155,312,863 (GRCm38) I344V probably benign Het
Zfp423 T A 8: 87,859,340 (GRCm38) Q61L possibly damaging Het
Other mutations in Gpr85
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02414:Gpr85 APN 6 13,836,910 (GRCm38) utr 5 prime probably benign
R0784:Gpr85 UTSW 6 13,836,749 (GRCm38) missense probably benign 0.25
R1356:Gpr85 UTSW 6 13,836,147 (GRCm38) missense probably benign 0.42
R2343:Gpr85 UTSW 6 13,836,696 (GRCm38) missense probably damaging 1.00
R3934:Gpr85 UTSW 6 13,836,045 (GRCm38) missense probably benign 0.02
R3935:Gpr85 UTSW 6 13,836,045 (GRCm38) missense probably benign 0.02
R3936:Gpr85 UTSW 6 13,836,045 (GRCm38) missense probably benign 0.02
R4925:Gpr85 UTSW 6 13,835,978 (GRCm38) missense probably benign 0.26
R5313:Gpr85 UTSW 6 13,836,302 (GRCm38) missense probably damaging 1.00
R7043:Gpr85 UTSW 6 13,835,877 (GRCm38) missense probably damaging 1.00
R8458:Gpr85 UTSW 6 13,836,849 (GRCm38) missense probably benign
R8468:Gpr85 UTSW 6 13,836,296 (GRCm38) missense probably damaging 1.00
R8503:Gpr85 UTSW 6 13,836,830 (GRCm38) missense probably benign 0.01
R9519:Gpr85 UTSW 6 13,836,999 (GRCm38) start gained probably benign
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-10-26