Incidental Mutation 'R4844:St8sia1'
ID 439478
Institutional Source Beutler Lab
Gene Symbol St8sia1
Ensembl Gene ENSMUSG00000030283
Gene Name ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms GD3S, GD3 synthase, Siat8, ST8Sia I, alpha-2,8-sialyltransferase, Siat8a, 9330109E03Rik
MMRRC Submission 042457-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.055) question?
Stock # R4844 (G1)
Quality Score 225
Status Validated
Chromosome 6
Chromosomal Location 142767271-142910178 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 142774996 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Serine at position 194 (R194S)
Ref Sequence ENSEMBL: ENSMUSP00000032421 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032421] [ENSMUST00000205149]
AlphaFold Q64687
Predicted Effect possibly damaging
Transcript: ENSMUST00000032421
AA Change: R194S

PolyPhen 2 Score 0.821 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000032421
Gene: ENSMUSG00000030283
AA Change: R194S

transmembrane domain 27 49 N/A INTRINSIC
Pfam:Glyco_transf_29 90 344 8.1e-73 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000100821
Predicted Effect noncoding transcript
Transcript: ENSMUST00000147058
Predicted Effect probably benign
Transcript: ENSMUST00000205149
SMART Domains Protein: ENSMUSP00000145148
Gene: ENSMUSG00000030283

transmembrane domain 27 49 N/A INTRINSIC
transmembrane domain 76 95 N/A INTRINSIC
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.7%
  • 20x: 93.6%
Validation Efficiency 97% (68/70)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2015]
PHENOTYPE: Homozygotes for a targeted allele are behaviorally normal with no signs of aberrant brain histology or demyelination. Homozygotes for a knock-out allele are behaviorally intact with normal nervous tissue morphology and sensitivity to Fas-mediated apoptosis but show impaired repair of damaged nerves. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcc9 T C 6: 142,634,824 (GRCm39) T147A probably benign Het
Acvrl1 T C 15: 101,033,409 (GRCm39) S99P probably damaging Het
Adamts3 T A 5: 89,825,675 (GRCm39) S1055C probably damaging Het
Aftph T C 11: 20,658,667 (GRCm39) probably benign Het
Aldh1a1 A G 19: 20,611,764 (GRCm39) K363E probably benign Het
Astn2 A G 4: 65,562,967 (GRCm39) V886A possibly damaging Het
Atad5 G A 11: 80,005,137 (GRCm39) probably null Het
C9orf72 A G 4: 35,213,565 (GRCm39) V31A possibly damaging Het
Ccnf G T 17: 24,449,331 (GRCm39) Y482* probably null Het
Cfap97 A G 8: 46,622,712 (GRCm39) D34G possibly damaging Het
Chst11 G T 10: 83,026,923 (GRCm39) E117* probably null Het
Cobl A T 11: 12,204,740 (GRCm39) L572Q probably benign Het
Coch G A 12: 51,649,477 (GRCm39) G263S probably damaging Het
Coq4 T C 2: 29,686,026 (GRCm39) I205T possibly damaging Het
Cyp3a13 A T 5: 137,915,813 (GRCm39) I62K probably benign Het
Cyp7a1 A G 4: 6,273,655 (GRCm39) S84P probably damaging Het
Dennd10 GTCT GT 19: 60,823,435 (GRCm39) probably null Het
G6pc3 G A 11: 102,084,057 (GRCm39) probably null Het
Gm10576 T C 4: 100,911,707 (GRCm39) noncoding transcript Het
Gm17511 G A 7: 126,885,454 (GRCm39) noncoding transcript Het
Gnat2 T C 3: 108,002,831 (GRCm39) S80P probably damaging Het
Gpaa1 C T 15: 76,216,508 (GRCm39) probably benign Het
Gpr107 T A 2: 31,078,686 (GRCm39) probably null Het
Hormad1 T C 3: 95,478,242 (GRCm39) Y103H probably damaging Het
Ighv14-1 T G 12: 113,895,622 (GRCm39) Q101P probably damaging Het
Igsf9 A G 1: 172,324,737 (GRCm39) D885G probably benign Het
Il15ra G A 2: 11,723,082 (GRCm39) probably benign Het
Islr2 C T 9: 58,115,517 (GRCm39) probably benign Het
Jak3 A T 8: 72,134,299 (GRCm39) N467I possibly damaging Het
Jarid2 T C 13: 45,067,248 (GRCm39) V1023A probably damaging Het
Kcnn2 A T 18: 45,816,187 (GRCm39) T333S possibly damaging Het
Klk14 G A 7: 43,341,501 (GRCm39) C51Y probably damaging Het
Kmt2c T C 5: 25,520,111 (GRCm39) T2000A probably benign Het
Lpxn A G 19: 12,810,536 (GRCm39) T327A probably damaging Het
Mxra8 A G 4: 155,927,151 (GRCm39) T362A probably benign Het
Myh6 A G 14: 55,184,651 (GRCm39) I1560T possibly damaging Het
Ndufv1 A G 19: 4,062,574 (GRCm39) S17P probably benign Het
Nwd1 A T 8: 73,393,742 (GRCm39) H335L probably damaging Het
Or1a1 A G 11: 74,086,902 (GRCm39) D191G probably damaging Het
Or2f1b T A 6: 42,739,394 (GRCm39) M136K probably damaging Het
Otop2 G A 11: 115,214,201 (GRCm39) probably null Het
Pwwp2b C A 7: 138,835,502 (GRCm39) S314R probably benign Het
Rfx7 C T 9: 72,500,524 (GRCm39) Q95* probably null Het
Serpine2 A T 1: 79,777,241 (GRCm39) L192* probably null Het
Smpdl3b T C 4: 132,465,369 (GRCm39) I322M probably damaging Het
Spata31d1b C T 13: 59,866,169 (GRCm39) R1106C possibly damaging Het
Sprr2k T G 3: 92,336,732 (GRCm39) probably null Het
Sv2a T A 3: 96,095,695 (GRCm39) V337D probably damaging Het
Tars1 G A 15: 11,385,281 (GRCm39) R637W possibly damaging Het
Tcp11l2 G T 10: 84,449,555 (GRCm39) V507L probably benign Het
Tpr T C 1: 150,321,630 (GRCm39) Y2262H possibly damaging Het
Vars1 A G 17: 35,230,588 (GRCm39) E529G probably damaging Het
Vash2 C T 1: 190,710,691 (GRCm39) probably benign Het
Vmn1r194 T C 13: 22,429,223 (GRCm39) V280A probably benign Het
Vti1a A G 19: 55,380,297 (GRCm39) T142A probably damaging Het
Zfp111 C T 7: 23,898,801 (GRCm39) C270Y probably damaging Het
Zfp518a T A 19: 40,903,340 (GRCm39) Y1090N probably damaging Het
Zfp616 A T 11: 73,975,225 (GRCm39) Y498F probably benign Het
Other mutations in St8sia1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02081:St8sia1 APN 6 142,774,953 (GRCm39) missense probably benign 0.00
IGL02138:St8sia1 APN 6 142,909,504 (GRCm39) utr 5 prime probably benign
IGL02419:St8sia1 APN 6 142,774,661 (GRCm39) missense probably damaging 1.00
IGL03407:St8sia1 APN 6 142,859,775 (GRCm39) missense possibly damaging 0.80
PIT4453001:St8sia1 UTSW 6 142,774,978 (GRCm39) nonsense probably null
PIT4498001:St8sia1 UTSW 6 142,859,848 (GRCm39) missense probably damaging 1.00
R0167:St8sia1 UTSW 6 142,859,907 (GRCm39) splice site probably benign
R0690:St8sia1 UTSW 6 142,774,980 (GRCm39) missense probably damaging 1.00
R1727:St8sia1 UTSW 6 142,822,453 (GRCm39) missense probably damaging 0.99
R1743:St8sia1 UTSW 6 142,774,742 (GRCm39) missense probably damaging 1.00
R1937:St8sia1 UTSW 6 142,909,398 (GRCm39) missense probably damaging 1.00
R2923:St8sia1 UTSW 6 142,774,963 (GRCm39) missense probably damaging 1.00
R2983:St8sia1 UTSW 6 142,909,355 (GRCm39) missense probably damaging 0.99
R3824:St8sia1 UTSW 6 142,774,751 (GRCm39) missense probably damaging 1.00
R4803:St8sia1 UTSW 6 142,813,649 (GRCm39) missense probably benign 0.04
R4865:St8sia1 UTSW 6 142,774,796 (GRCm39) missense probably damaging 1.00
R4886:St8sia1 UTSW 6 142,859,860 (GRCm39) missense probably damaging 0.99
R5170:St8sia1 UTSW 6 142,909,434 (GRCm39) missense probably damaging 0.99
R5519:St8sia1 UTSW 6 142,909,287 (GRCm39) missense probably damaging 0.99
R5783:St8sia1 UTSW 6 142,909,340 (GRCm39) missense possibly damaging 0.83
R6713:St8sia1 UTSW 6 142,775,008 (GRCm39) splice site probably null
R7017:St8sia1 UTSW 6 142,813,632 (GRCm39) missense probably damaging 0.98
R7144:St8sia1 UTSW 6 142,822,395 (GRCm39) missense probably damaging 1.00
R7997:St8sia1 UTSW 6 142,909,376 (GRCm39) missense probably damaging 1.00
Z1176:St8sia1 UTSW 6 142,774,825 (GRCm39) missense probably damaging 1.00
Z1177:St8sia1 UTSW 6 142,774,536 (GRCm39) nonsense probably null
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2016-11-02