Mutagenetix > Incidental Mutation

Incidental Mutation 'R5616:Vmn2r59'

439533

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r59

Ensembl Gene

ENSMUSG00000092032

Gene Name

vomeronasal 2, receptor 59

Synonyms

EG628444

MMRRC Submission

043159-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R5616 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41661216-41708405 bp(-) (GRCm39)

Type of Mutation

splice site (6 bp from exon)

DNA Base Change (assembly)

A to G at 41708191 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000131856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168489]

AlphaFold

E9PUT5

Predicted Effect

probably null
Transcript: ENSMUST00000168489

SMART Domains

Protein: ENSMUSP00000131856
Gene: ENSMUSG00000092032

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-44	PFAM
Pfam:NCD3G	514	567	4.3e-23	PFAM
Pfam:7tm_3	600	835	5.4e-53	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.4%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd3	A	T	3: 121,566,009 (GRCm39)	D443E	probably benign	Het
Arhgef5	A	G	6: 43,252,874 (GRCm39)	D1037G	probably benign	Het
B3glct	T	A	5: 149,653,399 (GRCm39)	L148*	probably null	Het
Cacna1e	G	A	1: 154,317,940 (GRCm39)	P1481S	probably damaging	Het
Cacna1h	C	A	17: 25,596,641 (GRCm39)	R117L	probably damaging	Het
Cct6b	A	T	11: 82,632,175 (GRCm39)	V271E	probably damaging	Het
Cenpf	A	G	1: 189,389,483 (GRCm39)	S1450P	probably damaging	Het
Cul1	G	A	6: 47,500,722 (GRCm39)	R707H	probably damaging	Het
Dcakd	A	G	11: 102,885,831 (GRCm39)	L191P	possibly damaging	Het
Dhx32	T	C	7: 133,322,957 (GRCm39)	*612W	probably null	Het
Disp1	T	A	1: 182,869,913 (GRCm39)	T836S	probably benign	Het
Dppa4	T	A	16: 48,111,393 (GRCm39)	V126D	probably damaging	Het
Fam98b	A	G	2: 117,098,267 (GRCm39)	T246A	possibly damaging	Het
Fkbp4	A	T	6: 128,410,517 (GRCm39)	V273E	probably damaging	Het
Gpr31b	A	G	17: 13,270,831 (GRCm39)	Y113H	probably damaging	Het
Gpr33	T	C	12: 52,070,377 (GRCm39)	I221V	probably damaging	Het
Klhl1	A	G	14: 96,755,729 (GRCm39)	F9L	probably benign	Het
Larp4b	T	A	13: 9,208,695 (GRCm39)	N449K	probably damaging	Het
Loxhd1	T	C	18: 77,492,647 (GRCm39)	S1363P	probably damaging	Het
Lrrc8a	T	C	2: 30,145,366 (GRCm39)	V60A	probably benign	Het
Matn3	A	G	12: 8,998,195 (GRCm39)	H51R	probably benign	Het
Mplkipl1	C	T	19: 61,164,364 (GRCm39)	G24R	unknown	Het
Mtrf1	T	C	14: 79,638,885 (GRCm39)	C6R	possibly damaging	Het
Myh6	T	C	14: 55,194,038 (GRCm39)	S784G	probably benign	Het
Nfkb2	G	T	19: 46,296,006 (GRCm39)	E170D	probably benign	Het
Nipal3	T	C	4: 135,179,715 (GRCm39)	Y401C	probably benign	Het
Pcdhb20	A	G	18: 37,637,585 (GRCm39)	E37G	probably damaging	Het
Pelp1	G	A	11: 70,285,688 (GRCm39)	P727S	possibly damaging	Het
Prkca	T	C	11: 107,869,169 (GRCm39)	D472G	possibly damaging	Het
Ptprg	A	G	14: 12,122,120 (GRCm38)	H328R	probably benign	Het
Rtl1	C	T	12: 109,559,173 (GRCm39)	E889K	unknown	Het
Rubcn	T	C	16: 32,647,293 (GRCm39)	D715G	possibly damaging	Het
Snurf	C	T	7: 59,649,133 (GRCm39)	E15K	possibly damaging	Het
Sptbn5	G	A	2: 119,876,965 (GRCm39)		probably benign	Het
Srp72	T	G	5: 77,135,781 (GRCm39)	L237R	probably damaging	Het
Syne3	T	C	12: 104,921,937 (GRCm39)	E406G	probably damaging	Het
Tgm2	A	G	2: 157,970,640 (GRCm39)	S340P	probably damaging	Het
Trim41	GCCTAGGCGCCCA	G	11: 48,698,192 (GRCm39)		probably benign	Het
Tsc22d1	T	C	14: 76,653,657 (GRCm39)		probably benign	Het
Tsc22d2	G	T	3: 58,324,583 (GRCm39)		probably benign	Het
Vmn1r42	T	C	6: 89,822,084 (GRCm39)	I162V	possibly damaging	Het
Wnt3	T	C	11: 103,703,596 (GRCm39)		probably null	Het

Other mutations in Vmn2r59

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Vmn2r59	APN	7	41,661,488 (GRCm39)	missense	possibly damaging	0.91
IGL01432:Vmn2r59	APN	7	41,661,983 (GRCm39)	missense	possibly damaging	0.82
IGL02119:Vmn2r59	APN	7	41,695,593 (GRCm39)	missense	probably benign	0.36
IGL02216:Vmn2r59	APN	7	41,661,817 (GRCm39)	missense	probably damaging	1.00
IGL02327:Vmn2r59	APN	7	41,661,655 (GRCm39)	missense	probably benign
IGL03346:Vmn2r59	APN	7	41,693,253 (GRCm39)	missense	probably benign	0.00
IGL03411:Vmn2r59	APN	7	41,708,340 (GRCm39)	missense	probably benign	0.43
IGL03412:Vmn2r59	APN	7	41,661,862 (GRCm39)	missense	probably benign
PIT4366001:Vmn2r59	UTSW	7	41,695,205 (GRCm39)	missense	possibly damaging	0.91
R0068:Vmn2r59	UTSW	7	41,695,725 (GRCm39)	missense	probably damaging	0.99
R0094:Vmn2r59	UTSW	7	41,661,722 (GRCm39)	missense	probably benign	0.07
R0179:Vmn2r59	UTSW	7	41,696,432 (GRCm39)	nonsense	probably null
R0370:Vmn2r59	UTSW	7	41,662,150 (GRCm39)	missense	probably benign	0.23
R0412:Vmn2r59	UTSW	7	41,695,916 (GRCm39)	splice site	probably benign
R0465:Vmn2r59	UTSW	7	41,696,332 (GRCm39)	missense	probably benign
R0487:Vmn2r59	UTSW	7	41,696,528 (GRCm39)	nonsense	probably null
R0576:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R0632:Vmn2r59	UTSW	7	41,708,308 (GRCm39)	missense	probably damaging	1.00
R1356:Vmn2r59	UTSW	7	41,661,218 (GRCm39)	makesense	probably null
R1387:Vmn2r59	UTSW	7	41,695,521 (GRCm39)	missense	probably damaging	1.00
R1388:Vmn2r59	UTSW	7	41,695,133 (GRCm39)	missense	probably benign	0.01
R1435:Vmn2r59	UTSW	7	41,695,629 (GRCm39)	missense	possibly damaging	0.50
R1750:Vmn2r59	UTSW	7	41,695,251 (GRCm39)	missense	possibly damaging	0.50
R2020:Vmn2r59	UTSW	7	41,693,203 (GRCm39)	missense	probably damaging	1.00
R2249:Vmn2r59	UTSW	7	41,708,326 (GRCm39)	missense	probably benign	0.00
R2256:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2257:Vmn2r59	UTSW	7	41,661,669 (GRCm39)	nonsense	probably null
R2441:Vmn2r59	UTSW	7	41,695,570 (GRCm39)	missense	probably benign	0.00
R2511:Vmn2r59	UTSW	7	41,693,190 (GRCm39)	missense	probably damaging	1.00
R2860:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R2861:Vmn2r59	UTSW	7	41,696,427 (GRCm39)	missense	possibly damaging	0.79
R3690:Vmn2r59	UTSW	7	41,661,370 (GRCm39)	missense	possibly damaging	0.77
R3912:Vmn2r59	UTSW	7	41,695,744 (GRCm39)	missense	probably benign	0.00
R4167:Vmn2r59	UTSW	7	41,670,732 (GRCm39)	intron	probably benign
R4357:Vmn2r59	UTSW	7	41,661,644 (GRCm39)	missense	probably damaging	1.00
R4445:Vmn2r59	UTSW	7	41,691,874 (GRCm39)	missense	probably damaging	1.00
R4542:Vmn2r59	UTSW	7	41,695,497 (GRCm39)	missense	possibly damaging	0.93
R4587:Vmn2r59	UTSW	7	41,695,648 (GRCm39)	missense	probably benign	0.00
R4616:Vmn2r59	UTSW	7	41,661,862 (GRCm39)	missense	probably benign
R4653:Vmn2r59	UTSW	7	41,693,228 (GRCm39)	missense	probably benign	0.19
R4703:Vmn2r59	UTSW	7	41,661,686 (GRCm39)	missense	probably benign	0.01
R4895:Vmn2r59	UTSW	7	41,695,218 (GRCm39)	missense	probably damaging	0.98
R4910:Vmn2r59	UTSW	7	41,693,077 (GRCm39)	missense	probably benign
R5045:Vmn2r59	UTSW	7	41,695,496 (GRCm39)	missense	possibly damaging	0.93
R5105:Vmn2r59	UTSW	7	41,696,529 (GRCm39)	missense	probably benign	0.01
R5153:Vmn2r59	UTSW	7	41,691,834 (GRCm39)	critical splice donor site	probably null
R5566:Vmn2r59	UTSW	7	41,696,247 (GRCm39)	missense	possibly damaging	0.92
R5586:Vmn2r59	UTSW	7	41,695,105 (GRCm39)	missense	probably benign	0.12
R5606:Vmn2r59	UTSW	7	41,695,318 (GRCm39)	missense	probably benign	0.27
R5625:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R5696:Vmn2r59	UTSW	7	41,695,468 (GRCm39)	missense	probably benign	0.00
R5982:Vmn2r59	UTSW	7	41,695,491 (GRCm39)	missense	probably benign	0.00
R6106:Vmn2r59	UTSW	7	41,661,749 (GRCm39)	nonsense	probably null
R6196:Vmn2r59	UTSW	7	41,661,679 (GRCm39)	missense	probably benign	0.36
R6228:Vmn2r59	UTSW	7	41,691,835 (GRCm39)	critical splice donor site	probably null
R6590:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6625:Vmn2r59	UTSW	7	41,693,177 (GRCm39)	missense	probably benign	0.02
R6690:Vmn2r59	UTSW	7	41,695,890 (GRCm39)	missense	probably damaging	1.00
R6768:Vmn2r59	UTSW	7	41,661,392 (GRCm39)	missense	probably benign	0.17
R6830:Vmn2r59	UTSW	7	41,693,171 (GRCm39)	missense	probably benign	0.10
R6859:Vmn2r59	UTSW	7	41,693,277 (GRCm39)	missense	probably damaging	1.00
R7034:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7036:Vmn2r59	UTSW	7	41,695,644 (GRCm39)	missense	probably benign	0.03
R7145:Vmn2r59	UTSW	7	41,695,188 (GRCm39)	missense	probably damaging	1.00
R7556:Vmn2r59	UTSW	7	41,695,233 (GRCm39)	missense	probably damaging	1.00
R7733:Vmn2r59	UTSW	7	41,661,443 (GRCm39)	missense	probably benign	0.17
R7770:Vmn2r59	UTSW	7	41,708,336 (GRCm39)	missense	probably damaging	1.00
R7812:Vmn2r59	UTSW	7	41,695,196 (GRCm39)	nonsense	probably null
R7867:Vmn2r59	UTSW	7	41,661,707 (GRCm39)	missense	probably damaging	1.00
R7975:Vmn2r59	UTSW	7	41,693,199 (GRCm39)	missense	probably damaging	1.00
R7999:Vmn2r59	UTSW	7	41,696,256 (GRCm39)	missense	probably damaging	1.00
R8267:Vmn2r59	UTSW	7	41,661,521 (GRCm39)	missense	probably damaging	0.97
R8367:Vmn2r59	UTSW	7	41,661,247 (GRCm39)	missense	probably benign	0.44
R9106:Vmn2r59	UTSW	7	41,695,884 (GRCm39)	missense	probably benign	0.03
R9135:Vmn2r59	UTSW	7	41,693,127 (GRCm39)	missense
R9135:Vmn2r59	UTSW	7	41,693,125 (GRCm39)	missense	probably benign	0.33
R9234:Vmn2r59	UTSW	7	41,661,907 (GRCm39)	missense	possibly damaging	0.67
R9273:Vmn2r59	UTSW	7	41,695,286 (GRCm39)	nonsense	probably null
R9432:Vmn2r59	UTSW	7	41,696,254 (GRCm39)	missense	probably damaging	1.00
R9433:Vmn2r59	UTSW	7	41,695,590 (GRCm39)	missense	probably damaging	0.99
R9616:Vmn2r59	UTSW	7	41,661,299 (GRCm39)	missense	probably damaging	1.00
R9654:Vmn2r59	UTSW	7	41,693,217 (GRCm39)	missense	probably benign	0.10
R9741:Vmn2r59	UTSW	7	41,708,209 (GRCm39)	missense	probably damaging	0.99
X0025:Vmn2r59	UTSW	7	41,695,365 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r59	UTSW	7	41,661,838 (GRCm39)	missense	possibly damaging	0.85
Z1176:Vmn2r59	UTSW	7	41,691,941 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTGGTCCATGCATGACAAAC -3'
(R):5'- CATCTGTCTCTTGGATGGGCAG -3'

Sequencing Primer
(F):5'- CATGCATGACAAACAAACGATTATGG -3'
(R):5'- TCTCTTGGATGGGCAGATATATATG -3'

Posted On

2016-11-08