Mutagenetix > Incidental Mutation

Incidental Mutation 'R5631:Mrgprf'

439858

Institutional Source

Beutler Lab

Gene Symbol

Mrgprf

Ensembl Gene

ENSMUSG00000031070

Gene Name

MAS-related GPR, member F

Synonyms

MrgF

MMRRC Submission

043282-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R5631 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144854646-144863294 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 144862283 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 282 (I282V)

Ref Sequence

ENSEMBL: ENSMUSP00000033386 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033386] [ENSMUST00000117718]

AlphaFold

Q8VCJ6

Predicted Effect

probably benign
Transcript: ENSMUST00000033386
AA Change: I282V

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000033386
Gene: ENSMUSG00000031070
AA Change: I282V

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	52	189	4.5e-7	PFAM
Pfam:7tm_1	61	291	2.4e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117718

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	C	A	11: 72,067,590 (GRCm39)	V567L	possibly damaging	Het
Abraxas1	T	G	5: 100,965,840 (GRCm39)	Y68S	probably damaging	Het
Adamtsl1	C	T	4: 86,195,160 (GRCm39)	Q543*	probably null	Het
Anapc1	T	C	2: 128,499,137 (GRCm39)	Y845C	possibly damaging	Het
Ap4m1	A	G	5: 138,173,051 (GRCm39)	*98W	probably null	Het
Clock	G	A	5: 76,378,185 (GRCm39)	P572S	probably benign	Het
Cramp1	T	A	17: 25,204,577 (GRCm39)	T275S	possibly damaging	Het
Dock3	T	C	9: 106,832,898 (GRCm39)	S1038G	probably benign	Het
Fancm	T	C	12: 65,160,617 (GRCm39)	V1397A	probably damaging	Het
Fn1	T	C	1: 71,629,355 (GRCm39)	T2203A	probably damaging	Het
Gm57858	G	C	3: 36,101,026 (GRCm39)	Q49E	probably damaging	Het
Heatr5a	T	A	12: 52,002,310 (GRCm39)	I209F	probably benign	Het
Hfm1	A	G	5: 107,052,629 (GRCm39)	S285P	probably damaging	Het
Hpx	A	T	7: 105,244,808 (GRCm39)	C126S	probably damaging	Het
Ipo4	A	G	14: 55,869,526 (GRCm39)	V378A	probably damaging	Het
Ipo4	C	T	14: 55,870,838 (GRCm39)	V265I	probably benign	Het
Kcnv1	T	A	15: 44,972,753 (GRCm39)	T377S	probably damaging	Het
Kmt2a	A	T	9: 44,731,985 (GRCm39)		probably benign	Het
Ldlrad3	T	C	2: 101,900,301 (GRCm39)	D67G	probably damaging	Het
Lrrc71	T	A	3: 87,646,456 (GRCm39)	M535L	probably benign	Het
Mfhas1	G	A	8: 36,055,573 (GRCm39)	R16Q	probably damaging	Het
Mrc1	A	T	2: 14,333,383 (GRCm39)	K1355*	probably null	Het
Mvb12b	G	T	2: 33,717,715 (GRCm39)	P142Q	probably damaging	Het
Naip6	T	C	13: 100,436,646 (GRCm39)	I626V	probably benign	Het
Ncoa5	T	C	2: 164,855,041 (GRCm39)	D27G	possibly damaging	Het
Nrap	T	G	19: 56,342,553 (GRCm39)	E780A	probably benign	Het
Oplah	T	C	15: 76,189,441 (GRCm39)	I228V	probably benign	Het
Or5w1b	A	T	2: 87,475,952 (GRCm39)	S172T	probably benign	Het
Pkdrej	T	C	15: 85,704,638 (GRCm39)	M433V	probably benign	Het
Polr3h	T	A	15: 81,810,113 (GRCm39)		probably benign	Het
Ppfibp1	T	C	6: 146,898,358 (GRCm39)	Y105H	probably damaging	Het
Rplp2	A	C	7: 141,031,172 (GRCm39)		probably benign	Het
Rps6ka4	T	C	19: 6,808,345 (GRCm39)		probably benign	Het
Rspry1	A	T	8: 95,355,706 (GRCm39)	M1L	possibly damaging	Het
Runx1	C	A	16: 92,492,451 (GRCm39)	R64L	possibly damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Slc9a5	C	T	8: 106,076,141 (GRCm39)	H45Y	possibly damaging	Het
Smc4	T	C	3: 68,937,645 (GRCm39)	I890T	probably benign	Het
Smtnl1	T	C	2: 84,649,098 (GRCm39)	E52G	probably benign	Het
Stag3	T	A	5: 138,294,139 (GRCm39)	I319N	probably damaging	Het
Stt3b	G	A	9: 115,083,913 (GRCm39)	T421I	probably benign	Het
Thumpd1	A	G	7: 119,319,825 (GRCm39)	L47P	probably damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Top2b	T	C	14: 16,409,882 (GRCm38)	Y850H	probably damaging	Het
Trim34a	A	T	7: 103,897,946 (GRCm39)	E158V	probably damaging	Het
Trp53i13	C	A	11: 77,400,419 (GRCm39)		probably null	Het
Ugt3a1	T	C	15: 9,361,971 (GRCm39)	V249A	probably damaging	Het
Vmn1r128	G	A	7: 21,083,300 (GRCm39)	M1I	probably null	Het
Yars1	T	G	4: 129,103,542 (GRCm39)	L297R	probably damaging	Het
Yju2b	G	T	8: 84,990,510 (GRCm39)	Q41K	probably damaging	Het
Zkscan3	A	G	13: 21,578,703 (GRCm39)	L176P	probably damaging	Het

Other mutations in Mrgprf

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0973:Mrgprf	UTSW	7	144,861,993 (GRCm39)	missense	probably damaging	0.96
R1709:Mrgprf	UTSW	7	144,861,954 (GRCm39)	missense	probably benign	0.07
R4643:Mrgprf	UTSW	7	144,862,242 (GRCm39)	missense	probably benign	0.39
R5965:Mrgprf	UTSW	7	144,861,168 (GRCm39)	intron	probably benign
R7147:Mrgprf	UTSW	7	144,862,128 (GRCm39)	missense	possibly damaging	0.59
R7291:Mrgprf	UTSW	7	144,861,206 (GRCm39)	missense	unknown
R7755:Mrgprf	UTSW	7	144,862,380 (GRCm39)	missense	probably damaging	1.00
R8826:Mrgprf	UTSW	7	144,862,046 (GRCm39)	missense	probably benign	0.16
R8881:Mrgprf	UTSW	7	144,861,999 (GRCm39)	missense	probably benign
R9112:Mrgprf	UTSW	7	144,861,503 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GTACAGTCTGCCGCAACATG -3'
(R):5'- CACTGCATTTCCATGGTGAC -3'

Sequencing Primer
(F):5'- GTCTGCCGCAACATGGACATC -3'
(R):5'- CATTTCCATGGTGACCGTGTTG -3'

Posted On

2016-11-08