Mutagenetix > Incidental Mutation

Incidental Mutation 'R5632:Bcl2l15'

439906

Institutional Source

Beutler Lab

Gene Symbol

Bcl2l15

Ensembl Gene

ENSMUSG00000044165

Gene Name

BCLl2-like 15

Synonyms

LOC229672, Bfk

MMRRC Submission

043283-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R5632 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

103739917-103761934 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 103743378 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 93 (N93S)

Ref Sequence

ENSEMBL: ENSMUSP00000102435 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062945] [ENSMUST00000106822] [ENSMUST00000199710]

AlphaFold

Q08ED0

Predicted Effect

probably benign
Transcript: ENSMUST00000062945
AA Change: N93S

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000052210
Gene: ENSMUSG00000044165
AA Change: N93S

Domain	Start	End	E-Value	Type
Blast:BCL	60	162	2e-54	BLAST
SCOP:d1k3ka_	61	165	5e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000106822
AA Change: N93S

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000102435
Gene: ENSMUSG00000044165
AA Change: N93S

Domain	Start	End	E-Value	Type
Blast:BCL	60	162	2e-54	BLAST
SCOP:d1maz__	60	162	1e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000199710

SMART Domains

Protein: ENSMUSP00000143463
Gene: ENSMUSG00000105053

Domain	Start	End	E-Value	Type
Pfam:Cnd1	35	212	5e-9	PFAM
Pfam:Adaptin_N	35	450	1.2e-62	PFAM
B2-adapt-app_C	544	656	3.75e-42	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd4	T	C	12: 84,664,076 (GRCm39)	T7A	probably benign	Het
Adam33	T	G	2: 130,895,362 (GRCm39)	D619A	probably damaging	Het
Ajm1	T	C	2: 25,469,276 (GRCm39)	T212A	probably benign	Het
Aldh3b3	C	T	19: 4,018,522 (GRCm39)		probably benign	Het
Arhgef15	G	A	11: 68,844,877 (GRCm39)	P240L	probably benign	Het
Arhgef40	C	A	14: 52,231,795 (GRCm39)	T727K	probably damaging	Het
Arrdc1	T	C	2: 24,817,840 (GRCm39)	T43A	probably benign	Het
Cd5l	G	T	3: 87,273,414 (GRCm39)	E128*	probably null	Het
Cep97	T	C	16: 55,735,946 (GRCm39)	D284G	probably benign	Het
Ckmt1	T	A	2: 121,191,073 (GRCm39)	S162T	probably damaging	Het
Clock	G	A	5: 76,378,185 (GRCm39)	P572S	probably benign	Het
Cpeb4	A	G	11: 31,839,877 (GRCm39)	D53G	probably damaging	Het
Crocc2	T	A	1: 93,145,575 (GRCm39)	S1485R	probably damaging	Het
Dcaf5	G	A	12: 80,444,526 (GRCm39)	A189V	probably damaging	Het
Duox2	C	T	2: 122,111,936 (GRCm39)	G1355S	probably damaging	Het
Fam120b	C	T	17: 15,623,344 (GRCm39)	P441S	probably benign	Het
Fbxo41	A	G	6: 85,461,486 (GRCm39)	L74P	probably damaging	Het
H2-DMa	A	G	17: 34,356,975 (GRCm39)	T158A	probably benign	Het
Hcar2	T	A	5: 124,002,532 (GRCm39)	T324S	probably benign	Het
Hif3a	T	C	7: 16,784,580 (GRCm39)	I222V	possibly damaging	Het
Ighv1-7	A	G	12: 114,502,501 (GRCm39)		probably benign	Het
Il20	T	C	1: 130,835,165 (GRCm39)	E151G	probably benign	Het
Jarid2	A	G	13: 45,049,766 (GRCm39)	E236G	probably damaging	Het
Knl1	T	C	2: 118,900,833 (GRCm39)	S845P	probably damaging	Het
Lamc2	T	C	1: 153,007,636 (GRCm39)	Y846C	probably damaging	Het
Lrp5	A	G	19: 3,672,512 (GRCm39)	V599A	probably benign	Het
Lrrc8b	T	A	5: 105,628,163 (GRCm39)	S170T	possibly damaging	Het
Mex3d	T	C	10: 80,218,428 (GRCm39)	K263R	probably damaging	Het
Mtor	A	G	4: 148,553,463 (GRCm39)	K784E	possibly damaging	Het
Naip5	C	A	13: 100,367,170 (GRCm39)		probably null	Het
Ncor1	T	C	11: 62,229,060 (GRCm39)	T609A	possibly damaging	Het
Ndufs1	C	T	1: 63,189,218 (GRCm39)	A536T	probably benign	Het
Neto1	A	G	18: 86,516,768 (GRCm39)	I362V	probably benign	Het
Nfatc3	T	C	8: 106,805,689 (GRCm39)	L178P	probably damaging	Het
Npdc1	G	A	2: 25,298,957 (GRCm39)	D284N	probably damaging	Het
Nsd3	C	T	8: 26,169,985 (GRCm39)	T707M	probably benign	Het
Or51f2	A	C	7: 102,527,004 (GRCm39)	S226R	probably benign	Het
Or5w1b	A	T	2: 87,475,573 (GRCm39)	V298E	probably damaging	Het
Or9g19	T	C	2: 85,600,613 (GRCm39)	V156A	probably benign	Het
Pcnx1	T	A	12: 81,964,504 (GRCm39)	S224T	probably damaging	Het
Peak1	T	C	9: 56,165,058 (GRCm39)	T957A	probably damaging	Het
Pex7	A	T	10: 19,764,483 (GRCm39)	D153E	probably damaging	Het
Plscr1	A	G	9: 92,148,477 (GRCm39)	E139G	probably damaging	Het
Psmb8	T	C	17: 34,420,214 (GRCm39)	Y269H	probably benign	Het
Rmc1	T	C	18: 12,304,640 (GRCm39)	F72L	possibly damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Sft2d2	T	C	1: 165,012,657 (GRCm39)	T80A	probably damaging	Het
Slc12a5	A	G	2: 164,829,141 (GRCm39)	I583M	possibly damaging	Het
Slc23a4	T	C	6: 34,933,957 (GRCm39)	M49V	probably benign	Het
Smarcb1	G	A	10: 75,740,252 (GRCm39)	Q309*	probably null	Het
Sphkap	A	T	1: 83,256,006 (GRCm39)	V294E	probably benign	Het
Stoml1	C	A	9: 58,160,653 (GRCm39)	P35Q	probably damaging	Het
Timm10b	G	C	7: 105,290,329 (GRCm39)	R42P	probably damaging	Het
Tnfrsf8	A	T	4: 145,019,203 (GRCm39)	S211T	possibly damaging	Het
Ttn	T	C	2: 76,536,164 (GRCm39)	T26738A	probably damaging	Het
Vps13d	A	T	4: 144,801,452 (GRCm39)	Y474N	probably damaging	Het
Wdr81	A	T	11: 75,336,732 (GRCm39)	F1552L	probably damaging	Het
Wee1	TCCCC	TCCC	7: 109,723,776 (GRCm39)		probably null	Het
Wnt7a	A	C	6: 91,371,637 (GRCm39)	Y108*	probably null	Het
Zfp512b	C	T	2: 181,227,461 (GRCm39)	R56K	probably benign	Het

Other mutations in Bcl2l15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01012:Bcl2l15	APN	3	103,740,730 (GRCm39)	missense	probably damaging	1.00
IGL03323:Bcl2l15	APN	3	103,740,719 (GRCm39)	missense	probably benign	0.00
R0655:Bcl2l15	UTSW	3	103,740,285 (GRCm39)	critical splice donor site	probably null
R0788:Bcl2l15	UTSW	3	103,740,794 (GRCm39)	critical splice donor site	probably null
R1721:Bcl2l15	UTSW	3	103,745,914 (GRCm39)	splice site	probably null
R1744:Bcl2l15	UTSW	3	103,745,856 (GRCm39)	missense	probably damaging	1.00
R1867:Bcl2l15	UTSW	3	103,745,914 (GRCm39)	splice site	probably null
R5850:Bcl2l15	UTSW	3	103,743,432 (GRCm39)	missense	possibly damaging	0.79
R7485:Bcl2l15	UTSW	3	103,740,729 (GRCm39)	missense	probably damaging	1.00
R7546:Bcl2l15	UTSW	3	103,740,203 (GRCm39)	missense	probably benign	0.07
R7982:Bcl2l15	UTSW	3	103,740,158 (GRCm39)	missense	probably damaging	1.00
R9455:Bcl2l15	UTSW	3	103,743,369 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CCTGAAAGTATGAACTCTGTAGGTG -3'
(R):5'- TGGATAAACTCGCGGATGGC -3'

Sequencing Primer
(F):5'- atttgtccatctgccctc -3'
(R):5'- GCTGTGTTCCCGTTGATCATGC -3'

Posted On

2016-11-08