Mutagenetix > Incidental Mutation

Incidental Mutation 'R5632:Wee1'

439921

Institutional Source

Beutler Lab

Gene Symbol

Wee1

Ensembl Gene

ENSMUSG00000031016

Gene Name

WEE 1 homolog 1 (S. pombe)

Synonyms

Wee1A

MMRRC Submission

043283-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5632 (G1)

Quality Score

165

Status

Not validated

Chromosome

Chromosomal Location

109721266-109742506 bp(+) (GRCm39)

Type of Mutation

frame shift

DNA Base Change (assembly)

TCCCC to TCCC at 109723776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000033326 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033326]

AlphaFold

P47810

Predicted Effect

probably null
Transcript: ENSMUST00000033326

SMART Domains

Protein: ENSMUSP00000033326
Gene: ENSMUSG00000031016

Domain	Start	End	E-Value	Type
low complexity region	62	112	N/A	INTRINSIC
Pfam:Pkinase_Tyr	298	566	1.9e-26	PFAM
Pfam:Pkinase	298	568	1.7e-61	PFAM
low complexity region	620	631	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185931

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000210940

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.4%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption may exhibit embryonic lethality at E7. Mice homozygous for a knock-out allele exhibit lethality between E3.5 and E7.5 with reduced proliferation, increased apoptosis and abnormal G2/M checkpoint function. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd4	T	C	12: 84,664,076 (GRCm39)	T7A	probably benign	Het
Adam33	T	G	2: 130,895,362 (GRCm39)	D619A	probably damaging	Het
Ajm1	T	C	2: 25,469,276 (GRCm39)	T212A	probably benign	Het
Aldh3b3	C	T	19: 4,018,522 (GRCm39)		probably benign	Het
Arhgef15	G	A	11: 68,844,877 (GRCm39)	P240L	probably benign	Het
Arhgef40	C	A	14: 52,231,795 (GRCm39)	T727K	probably damaging	Het
Arrdc1	T	C	2: 24,817,840 (GRCm39)	T43A	probably benign	Het
Bcl2l15	A	G	3: 103,743,378 (GRCm39)	N93S	probably benign	Het
Cd5l	G	T	3: 87,273,414 (GRCm39)	E128*	probably null	Het
Cep97	T	C	16: 55,735,946 (GRCm39)	D284G	probably benign	Het
Ckmt1	T	A	2: 121,191,073 (GRCm39)	S162T	probably damaging	Het
Clock	G	A	5: 76,378,185 (GRCm39)	P572S	probably benign	Het
Cpeb4	A	G	11: 31,839,877 (GRCm39)	D53G	probably damaging	Het
Crocc2	T	A	1: 93,145,575 (GRCm39)	S1485R	probably damaging	Het
Dcaf5	G	A	12: 80,444,526 (GRCm39)	A189V	probably damaging	Het
Duox2	C	T	2: 122,111,936 (GRCm39)	G1355S	probably damaging	Het
Fam120b	C	T	17: 15,623,344 (GRCm39)	P441S	probably benign	Het
Fbxo41	A	G	6: 85,461,486 (GRCm39)	L74P	probably damaging	Het
H2-DMa	A	G	17: 34,356,975 (GRCm39)	T158A	probably benign	Het
Hcar2	T	A	5: 124,002,532 (GRCm39)	T324S	probably benign	Het
Hif3a	T	C	7: 16,784,580 (GRCm39)	I222V	possibly damaging	Het
Ighv1-7	A	G	12: 114,502,501 (GRCm39)		probably benign	Het
Il20	T	C	1: 130,835,165 (GRCm39)	E151G	probably benign	Het
Jarid2	A	G	13: 45,049,766 (GRCm39)	E236G	probably damaging	Het
Knl1	T	C	2: 118,900,833 (GRCm39)	S845P	probably damaging	Het
Lamc2	T	C	1: 153,007,636 (GRCm39)	Y846C	probably damaging	Het
Lrp5	A	G	19: 3,672,512 (GRCm39)	V599A	probably benign	Het
Lrrc8b	T	A	5: 105,628,163 (GRCm39)	S170T	possibly damaging	Het
Mex3d	T	C	10: 80,218,428 (GRCm39)	K263R	probably damaging	Het
Mtor	A	G	4: 148,553,463 (GRCm39)	K784E	possibly damaging	Het
Naip5	C	A	13: 100,367,170 (GRCm39)		probably null	Het
Ncor1	T	C	11: 62,229,060 (GRCm39)	T609A	possibly damaging	Het
Ndufs1	C	T	1: 63,189,218 (GRCm39)	A536T	probably benign	Het
Neto1	A	G	18: 86,516,768 (GRCm39)	I362V	probably benign	Het
Nfatc3	T	C	8: 106,805,689 (GRCm39)	L178P	probably damaging	Het
Npdc1	G	A	2: 25,298,957 (GRCm39)	D284N	probably damaging	Het
Nsd3	C	T	8: 26,169,985 (GRCm39)	T707M	probably benign	Het
Or51f2	A	C	7: 102,527,004 (GRCm39)	S226R	probably benign	Het
Or5w1b	A	T	2: 87,475,573 (GRCm39)	V298E	probably damaging	Het
Or9g19	T	C	2: 85,600,613 (GRCm39)	V156A	probably benign	Het
Pcnx1	T	A	12: 81,964,504 (GRCm39)	S224T	probably damaging	Het
Peak1	T	C	9: 56,165,058 (GRCm39)	T957A	probably damaging	Het
Pex7	A	T	10: 19,764,483 (GRCm39)	D153E	probably damaging	Het
Plscr1	A	G	9: 92,148,477 (GRCm39)	E139G	probably damaging	Het
Psmb8	T	C	17: 34,420,214 (GRCm39)	Y269H	probably benign	Het
Rmc1	T	C	18: 12,304,640 (GRCm39)	F72L	possibly damaging	Het
Secisbp2l	C	T	2: 125,582,657 (GRCm39)	G933D	possibly damaging	Het
Sft2d2	T	C	1: 165,012,657 (GRCm39)	T80A	probably damaging	Het
Slc12a5	A	G	2: 164,829,141 (GRCm39)	I583M	possibly damaging	Het
Slc23a4	T	C	6: 34,933,957 (GRCm39)	M49V	probably benign	Het
Smarcb1	G	A	10: 75,740,252 (GRCm39)	Q309*	probably null	Het
Sphkap	A	T	1: 83,256,006 (GRCm39)	V294E	probably benign	Het
Stoml1	C	A	9: 58,160,653 (GRCm39)	P35Q	probably damaging	Het
Timm10b	G	C	7: 105,290,329 (GRCm39)	R42P	probably damaging	Het
Tnfrsf8	A	T	4: 145,019,203 (GRCm39)	S211T	possibly damaging	Het
Ttn	T	C	2: 76,536,164 (GRCm39)	T26738A	probably damaging	Het
Vps13d	A	T	4: 144,801,452 (GRCm39)	Y474N	probably damaging	Het
Wdr81	A	T	11: 75,336,732 (GRCm39)	F1552L	probably damaging	Het
Wnt7a	A	C	6: 91,371,637 (GRCm39)	Y108*	probably null	Het
Zfp512b	C	T	2: 181,227,461 (GRCm39)	R56K	probably benign	Het

Other mutations in Wee1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00693:Wee1	APN	7	109,734,060 (GRCm39)	splice site	probably null
IGL00981:Wee1	APN	7	109,738,876 (GRCm39)	missense	probably damaging	1.00
IGL01017:Wee1	APN	7	109,725,055 (GRCm39)	missense	possibly damaging	0.93
IGL01357:Wee1	APN	7	109,741,242 (GRCm39)	missense	probably benign	0.39
IGL01838:Wee1	APN	7	109,723,744 (GRCm39)	missense	probably benign	0.01
IGL01970:Wee1	APN	7	109,738,457 (GRCm39)	missense	probably damaging	1.00
IGL02396:Wee1	APN	7	109,741,300 (GRCm39)	missense	probably damaging	1.00
IGL02511:Wee1	APN	7	109,738,483 (GRCm39)	missense	possibly damaging	0.55
IGL02884:Wee1	APN	7	109,725,269 (GRCm39)	missense	probably benign	0.02
IGL03085:Wee1	APN	7	109,723,805 (GRCm39)	missense	probably damaging	1.00
IGL03221:Wee1	APN	7	109,726,024 (GRCm39)	missense	probably damaging	1.00
IGL03383:Wee1	APN	7	109,738,899 (GRCm39)	missense	probably damaging	1.00
R0220:Wee1	UTSW	7	109,723,733 (GRCm39)	missense	probably benign	0.10
R1934:Wee1	UTSW	7	109,721,698 (GRCm39)	missense	probably benign	0.06
R3110:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3112:Wee1	UTSW	7	109,730,043 (GRCm39)	missense	probably damaging	1.00
R3978:Wee1	UTSW	7	109,723,762 (GRCm39)	missense	probably damaging	1.00
R4348:Wee1	UTSW	7	109,730,165 (GRCm39)	missense	probably damaging	1.00
R5434:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5435:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5436:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5449:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5566:Wee1	UTSW	7	109,725,257 (GRCm39)	nonsense	probably null
R5630:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5685:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5694:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5807:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R5941:Wee1	UTSW	7	109,723,776 (GRCm39)	frame shift	probably null
R6044:Wee1	UTSW	7	109,738,513 (GRCm39)	missense	probably benign	0.00
R6163:Wee1	UTSW	7	109,734,858 (GRCm39)	missense	probably damaging	1.00
R6826:Wee1	UTSW	7	109,723,870 (GRCm39)	critical splice donor site	probably null
R7203:Wee1	UTSW	7	109,734,001 (GRCm39)	missense	probably benign	0.00
R7835:Wee1	UTSW	7	109,730,085 (GRCm39)	nonsense	probably null
R8273:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R8953:Wee1	UTSW	7	109,723,691 (GRCm39)	missense	probably benign	0.00
R9077:Wee1	UTSW	7	109,725,963 (GRCm39)	missense	probably damaging	1.00
R9336:Wee1	UTSW	7	109,721,689 (GRCm39)	missense	probably damaging	1.00
R9463:Wee1	UTSW	7	109,721,917 (GRCm39)	missense	probably damaging	1.00
R9673:Wee1	UTSW	7	109,725,210 (GRCm39)	missense	probably damaging	0.98
R9748:Wee1	UTSW	7	109,721,722 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCACTTAGGAAGGCAAGTTGG -3'
(R):5'- GCCATTCAGTAGAAACCTAATTGTG -3'

Sequencing Primer
(F):5'- GTTGGAGGTTATATAGAGAAGACTCC -3'
(R):5'- AACCTAATTGTGTGCCAGGC -3'

Posted On

2016-11-08