Mutagenetix > Incidental Mutation

Incidental Mutation 'V1024:Vmo1'

44019

Institutional Source

Beutler Lab

Gene Symbol

Vmo1

Ensembl Gene

ENSMUSG00000020830

Gene Name

vitelline membrane outer layer 1 homolog (chicken)

Synonyms

LOC327956

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.089) question?

Stock #

V1024 () of strain 599

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

70404342-70405442 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 70404572 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 143 (F143S)

Ref Sequence

ENSEMBL: ENSMUSP00000021179 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019063] [ENSMUST00000021179]

AlphaFold

Q5SXG7

Predicted Effect

probably benign
Transcript: ENSMUST00000019063

SMART Domains

Protein: ENSMUSP00000019063
Gene: ENSMUSG00000018919

Domain	Start	End	E-Value	Type
Pfam:L6_membrane	1	189	3.8e-72	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000021179
AA Change: F143S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000021179
Gene: ENSMUSG00000020830
AA Change: F143S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:VOMI	35	199	6.2e-64	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000122307

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000141924

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000179986

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.6%
20x: 93.8%

Validation Efficiency

50% (2/4)

Allele List at MGI

Other mutations in this stock

Total: 11 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Dido1	G	T	2: 180,330,807 (GRCm39)	Q214K	probably benign	Het
Itgbl1	A	G	14: 124,210,699 (GRCm39)	N342D	probably damaging	Het
Loxl3	A	T	6: 83,012,719 (GRCm39)	E86V	probably damaging	Het
Phf2	C	T	13: 48,957,841 (GRCm39)	A1058T	possibly damaging	Het
Plxna4	G	A	6: 32,211,509 (GRCm39)	R677W	probably damaging	Het
Pnmt	G	A	11: 98,278,513 (GRCm39)	A160T	probably benign	Het
Rab24	C	T	13: 55,468,561 (GRCm39)	D144N	probably benign	Het
Slc7a1	C	T	5: 148,271,355 (GRCm39)	V535M	probably benign	Het
Stk32b	T	C	5: 37,614,578 (GRCm39)	D310G	probably damaging	Het
Sult3a2	A	T	10: 33,642,474 (GRCm39)	H276Q	probably benign	Het
Zc3h18	TGAGGAGGAGGAGGAG	TGAGGAGGAGGAG	8: 123,110,596 (GRCm39)		probably benign	Het

Other mutations in Vmo1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Vmo1	APN	11	70,404,424 (GRCm39)	missense	probably damaging	1.00
IGL03255:Vmo1	APN	11	70,405,236 (GRCm39)	critical splice donor site	probably null
H8441:Vmo1	UTSW	11	70,404,572 (GRCm39)	missense	probably damaging	1.00
PIT4791001:Vmo1	UTSW	11	70,404,661 (GRCm39)	missense	probably damaging	1.00
R2159:Vmo1	UTSW	11	70,404,608 (GRCm39)	missense	probably benign	0.03
R5913:Vmo1	UTSW	11	70,405,241 (GRCm39)	missense	probably damaging	0.98
R7139:Vmo1	UTSW	11	70,404,674 (GRCm39)	missense	probably benign	0.03
R8200:Vmo1	UTSW	11	70,405,325 (GRCm39)	missense	possibly damaging	0.74
R8960:Vmo1	UTSW	11	70,404,476 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmo1	UTSW	11	70,404,643 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAATCAAGGGGACAGCAACGCC -3'
(R):5'- TGCCCTTCCCTGAGATAAACTCCAC -3'

Sequencing Primer
(F):5'- AGTACTGGCAGAGCGACC -3'
(R):5'- ACTCCACTAAGGTTATAGAGGTGTG -3'

Posted On

2013-05-31