|Institutional Source||Beutler Lab|
|Gene Name||APOBEC1 complementation factor|
|Synonyms||apobec-1 complementation factor, ACF, 1810073H04Rik|
|Is this an essential gene?||Probably non essential (E-score: 0.070)|
|Stock #||R5636 (G1)|
|Chromosomal Location||31868764-31948573 bp(+) (GRCm38)|
|Type of Mutation||nonsense|
|DNA Base Change (assembly)||C to T at 31944982 bp (GRCm38)|
|Amino Acid Change||Glutamine to Stop codon at position 448 (Q448*)|
|Ref Sequence||ENSEMBL: ENSMUSP00000153542 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000075838] [ENSMUST00000224304]|
AA Change: Q456*
AA Change: Q456*
AA Change: Q448*
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
PHENOTYPE: Embryos homozygous for a targeted deletion of this gene are detectable only until the blastocyst stage (E3.5) and isolated mutant blastocysts fail to proliferate in vitro. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in A1cf||
(F):5'- TGGAGTCCAGGTTAAGTTCAAC -3'
(R):5'- ATTTAATTACAAGGGAGTGGGCTG -3'
(F):5'- TCTGAAGTTCAGAGCTCG -3'
(R):5'- GTTATTGGAGTGATGATCAAACACAG -3'